Myotis brandtii (Brandt s bat)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19243 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S7NC99|S7NC99_MYOBR Nucleolar protein 3 OS=Myotis brandtii OX=109478 GN=D623_10015045 PE=4 SV=1
MM1 pKa = 7.89 ADD3 pKa = 3.66 GVDD6 pKa = 4.52 HH7 pKa = 7.16 IDD9 pKa = 3.11 IYY11 pKa = 11.64 ADD13 pKa = 3.11 VGEE16 pKa = 4.75 EE17 pKa = 3.93 FNQEE21 pKa = 3.7 AEE23 pKa = 4.41 YY24 pKa = 10.89 GGHH27 pKa = 6.25 DD28 pKa = 5.19 QIDD31 pKa = 4.31 LYY33 pKa = 11.46 DD34 pKa = 4.25 DD35 pKa = 4.56 VISPSANNGDD45 pKa = 3.65 APEE48 pKa = 4.73 DD49 pKa = 3.79 RR50 pKa = 11.84 DD51 pKa = 4.66 YY52 pKa = 11.42 MDD54 pKa = 4.44 TLPPTVGDD62 pKa = 4.07 DD63 pKa = 3.68 VGKK66 pKa = 10.24 GAAPNVVYY74 pKa = 9.46 TYY76 pKa = 8.84 TGKK79 pKa = 10.88 RR80 pKa = 11.84 IALYY84 pKa = 9.95 IGNLTWVSKK93 pKa = 9.27 LTII96 pKa = 3.82
Molecular weight: 10.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.541
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.757
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|S7MQX8|S7MQX8_MYOBR Fibroblast growth factor OS=Myotis brandtii OX=109478 GN=D623_10014353 PE=3 SV=1
MM1 pKa = 7.49 SSSLHH6 pKa = 5.42 TRR8 pKa = 11.84 SSSLHH13 pKa = 5.7 KK14 pKa = 10.35 RR15 pKa = 11.84 SPSLHH20 pKa = 6.3 KK21 pKa = 10.46 KK22 pKa = 10.23 SPSLHH27 pKa = 6.01 KK28 pKa = 10.42 RR29 pKa = 11.84 SPSLHH34 pKa = 6.08 KK35 pKa = 10.41 RR36 pKa = 11.84 SPSLHH41 pKa = 6.4 RR42 pKa = 11.84 SSSSLHH48 pKa = 5.88 KK49 pKa = 10.4 RR50 pKa = 11.84 SPSLHH55 pKa = 6.3 KK56 pKa = 10.46 KK57 pKa = 10.23 SPSLHH62 pKa = 6.01 KK63 pKa = 10.42 RR64 pKa = 11.84 SPSLHH69 pKa = 6.3 KK70 pKa = 10.46 KK71 pKa = 10.23 SPSLHH76 pKa = 6.01 KK77 pKa = 10.42 RR78 pKa = 11.84 SPSLHH83 pKa = 6.3 KK84 pKa = 10.46 KK85 pKa = 10.23 SPSLHH90 pKa = 6.01 KK91 pKa = 10.42 RR92 pKa = 11.84 SPSLHH97 pKa = 6.3 KK98 pKa = 10.46 KK99 pKa = 10.23 SPSLHH104 pKa = 6.01 KK105 pKa = 10.42 RR106 pKa = 11.84 SPSLHH111 pKa = 6.3 KK112 pKa = 10.46 KK113 pKa = 10.23 SPSLHH118 pKa = 6.01 KK119 pKa = 10.42 RR120 pKa = 11.84 SPSLHH125 pKa = 6.3 KK126 pKa = 10.46 KK127 pKa = 10.23 SPSLHH132 pKa = 6.01 KK133 pKa = 10.42 RR134 pKa = 11.84 SPSLHH139 pKa = 6.3 KK140 pKa = 10.46 KK141 pKa = 10.23 SPSLHH146 pKa = 6.01 KK147 pKa = 10.42 RR148 pKa = 11.84 SPSLHH153 pKa = 6.3 KK154 pKa = 10.46 KK155 pKa = 10.23 SPSLHH160 pKa = 6.01 KK161 pKa = 10.42 RR162 pKa = 11.84 SPSLHH167 pKa = 6.3 KK168 pKa = 10.46 KK169 pKa = 10.23 SPSLHH174 pKa = 6.01 KK175 pKa = 10.42 RR176 pKa = 11.84 SPSLHH181 pKa = 6.3 KK182 pKa = 10.46 KK183 pKa = 10.23 SPSLHH188 pKa = 6.01 KK189 pKa = 10.42 RR190 pKa = 11.84 SPSLHH195 pKa = 6.3 KK196 pKa = 10.46 KK197 pKa = 10.23 SPSLHH202 pKa = 6.01 KK203 pKa = 10.42 RR204 pKa = 11.84 SPSLHH209 pKa = 6.08 KK210 pKa = 10.46 RR211 pKa = 11.84 SPSLHH216 pKa = 6.09 KK217 pKa = 10.46 RR218 pKa = 11.84 SSSLHH223 pKa = 5.57 KK224 pKa = 10.34 RR225 pKa = 11.84 SPSLHH230 pKa = 5.39 KK231 pKa = 10.41 RR232 pKa = 11.84 KK233 pKa = 8.65 MRR235 pKa = 11.84 KK236 pKa = 8.74 RR237 pKa = 3.42
Molecular weight: 27.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.412
IPC2_protein 11.652
IPC_protein 13.188
Toseland 13.364
ProMoST 13.846
Dawson 13.364
Bjellqvist 13.349
Wikipedia 13.832
Rodwell 13.32
Grimsley 13.408
Solomon 13.846
Lehninger 13.759
Nozaki 13.364
DTASelect 13.349
Thurlkill 13.364
EMBOSS 13.861
Sillero 13.364
Patrickios 13.027
IPC_peptide 13.861
IPC2_peptide 12.837
IPC2.peptide.svr19 9.447
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19243
0
19243
9174729
29
26686
476.8
53.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.862 ± 0.017
2.219 ± 0.014
4.854 ± 0.013
7.18 ± 0.025
3.674 ± 0.013
6.411 ± 0.022
2.639 ± 0.01
4.394 ± 0.016
5.852 ± 0.023
9.989 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.009
3.621 ± 0.013
6.118 ± 0.028
4.808 ± 0.018
5.618 ± 0.015
8.201 ± 0.024
5.319 ± 0.013
6.131 ± 0.018
1.226 ± 0.007
2.663 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here