Methylocystis bryophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylocystis

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6MTY1|A0A1W6MTY1_9RHIZ Fatty acid hydroxylase OS=Methylocystis bryophila OX=655015 GN=B1812_07540 PE=4 SV=1
MM1 pKa = 7.29NAQHH5 pKa = 7.06IYY7 pKa = 10.75ASNIEE12 pKa = 4.11QLSSEE17 pKa = 4.29DD18 pKa = 2.8GGYY21 pKa = 9.52WVVSFPDD28 pKa = 3.22IPGCLGVGDD37 pKa = 4.41SMAEE41 pKa = 3.98AEE43 pKa = 4.89ADD45 pKa = 3.6AQNALAACLDD55 pKa = 3.55AFKK58 pKa = 10.8AIDD61 pKa = 4.0RR62 pKa = 11.84PAPIPSEE69 pKa = 4.06RR70 pKa = 11.84PEE72 pKa = 3.75

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6N0M1|A0A1W6N0M1_9RHIZ Uncharacterized protein OS=Methylocystis bryophila OX=655015 GN=B1812_03495 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.28TVGGRR28 pKa = 11.84RR29 pKa = 11.84VVAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4267

0

4267

1300618

25

3363

304.8

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.179 ± 0.058

0.98 ± 0.016

5.228 ± 0.024

6.112 ± 0.036

3.995 ± 0.026

8.359 ± 0.046

1.977 ± 0.018

4.717 ± 0.027

3.429 ± 0.033

10.526 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.018

2.577 ± 0.03

5.42 ± 0.032

3.005 ± 0.023

7.458 ± 0.044

5.799 ± 0.031

4.78 ± 0.033

6.889 ± 0.03

1.311 ± 0.017

2.262 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski