Bacillus phage VMY22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Mingyongvirus; Bacillus virus VMY22

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9RRG0|A0A0N9RRG0_9CAUD NAGPA domain-containing protein OS=Bacillus phage VMY22 OX=1734382 GN=VMY22_17 PE=4 SV=1
MM1 pKa = 7.01VQLKK5 pKa = 10.3QYY7 pKa = 10.51DD8 pKa = 4.09FKK10 pKa = 11.72GKK12 pKa = 9.33IAGSVVEE19 pKa = 4.72NKK21 pKa = 10.48AIAYY25 pKa = 9.92LNDD28 pKa = 3.2VEE30 pKa = 4.95FTQEE34 pKa = 3.94QYY36 pKa = 11.62DD37 pKa = 3.87GVYY40 pKa = 8.74NSSMIVPAEE49 pKa = 3.75STIRR53 pKa = 11.84MEE55 pKa = 5.76FPIEE59 pKa = 3.98EE60 pKa = 4.19NKK62 pKa = 10.84GVIQTVSNYY71 pKa = 6.98FTGKK75 pKa = 9.64IEE77 pKa = 4.52SGTITLAVGDD87 pKa = 3.82YY88 pKa = 10.96AVYY91 pKa = 10.5NRR93 pKa = 11.84SGEE96 pKa = 4.38FKK98 pKa = 10.84QSMLDD103 pKa = 3.61VNISEE108 pKa = 4.26MTDD111 pKa = 2.9NKK113 pKa = 10.65IVMTFTATKK122 pKa = 9.11EE123 pKa = 3.87YY124 pKa = 10.77EE125 pKa = 4.3FYY127 pKa = 9.87RR128 pKa = 11.84TLLTVLYY135 pKa = 10.1DD136 pKa = 3.58DD137 pKa = 4.48EE138 pKa = 6.83AINRR142 pKa = 11.84YY143 pKa = 7.43TGVVSYY149 pKa = 10.57LQDD152 pKa = 2.97GNMITEE158 pKa = 4.54TLNSLVFTDD167 pKa = 6.09LISKK171 pKa = 8.13LQSYY175 pKa = 9.49PEE177 pKa = 3.92NSIDD181 pKa = 3.41NSTFSKK187 pKa = 10.63VPYY190 pKa = 9.77EE191 pKa = 4.15GVEE194 pKa = 3.86

Molecular weight: 21.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9SK16|A0A0N9SK16_9CAUD Upper collar protein OS=Bacillus phage VMY22 OX=1734382 GN=VMY22_15 PE=4 SV=1
MM1 pKa = 7.27EE2 pKa = 5.74HH3 pKa = 6.47KK4 pKa = 10.56QMHH7 pKa = 5.2TRR9 pKa = 11.84MGEE12 pKa = 3.83RR13 pKa = 11.84NDD15 pKa = 3.63RR16 pKa = 11.84RR17 pKa = 11.84NHH19 pKa = 5.66HH20 pKa = 7.32PITKK24 pKa = 8.02KK25 pKa = 8.97TKK27 pKa = 10.48GEE29 pKa = 3.98IEE31 pKa = 3.87MKK33 pKa = 8.03MTNEE37 pKa = 4.76DD38 pKa = 4.0YY39 pKa = 11.37LFLLKK44 pKa = 10.12QCKK47 pKa = 9.53DD48 pKa = 3.03KK49 pKa = 11.62YY50 pKa = 10.63LISDD54 pKa = 4.23FGKK57 pKa = 10.1YY58 pKa = 9.92LQVTQLIIVSFEE70 pKa = 4.0TGDD73 pKa = 3.17KK74 pKa = 10.79HH75 pKa = 7.24LLQRR79 pKa = 11.84TIIIYY84 pKa = 7.91EE85 pKa = 4.03LKK87 pKa = 10.56EE88 pKa = 3.83RR89 pKa = 11.84AA90 pKa = 3.48

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5786

54

592

231.4

26.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.528 ± 0.378

0.639 ± 0.142

6.135 ± 0.224

7.57 ± 0.453

4.753 ± 0.319

6.222 ± 0.475

2.212 ± 0.179

6.706 ± 0.313

8.071 ± 0.38

6.706 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.387 ± 0.186

6.568 ± 0.416

3.284 ± 0.231

3.854 ± 0.233

4.2 ± 0.374

5.773 ± 0.302

6.931 ± 0.414

6.326 ± 0.402

1.486 ± 0.235

4.649 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski