Giant panda associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; unclassified Gemycircularvirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A220IGP9|A0A220IGP9_9VIRU Replication-associated protein OS=Giant panda associated gemycircularvirus OX=2016461 PE=3 SV=1
MM1 pKa = 7.41SFAFNSRR8 pKa = 11.84YY9 pKa = 10.22VLLTYY14 pKa = 10.11AQSDD18 pKa = 4.16GLSEE22 pKa = 4.04WDD24 pKa = 3.58VLEE27 pKa = 5.2HH28 pKa = 6.83ISGLGAEE35 pKa = 4.78CIIARR40 pKa = 11.84EE41 pKa = 3.98NHH43 pKa = 6.51ADD45 pKa = 3.66GGTHH49 pKa = 6.67LHH51 pKa = 6.68AFCDD55 pKa = 4.96FGRR58 pKa = 11.84KK59 pKa = 8.2FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84SDD66 pKa = 2.88IFDD69 pKa = 3.12VGGRR73 pKa = 11.84HH74 pKa = 6.6ANITPSRR81 pKa = 11.84GRR83 pKa = 11.84PEE85 pKa = 4.69FGWDD89 pKa = 3.35YY90 pKa = 11.26AVKK93 pKa = 10.74DD94 pKa = 3.75GDD96 pKa = 4.06VVAGGLARR104 pKa = 11.84PGSGGLPEE112 pKa = 5.6APNKK116 pKa = 8.8WGQIVGAEE124 pKa = 4.02NEE126 pKa = 4.12QQFWEE131 pKa = 4.44LVEE134 pKa = 4.34EE135 pKa = 4.79LDD137 pKa = 4.03PKK139 pKa = 11.1ALATQYY145 pKa = 10.76PSLRR149 pKa = 11.84KK150 pKa = 8.97FADD153 pKa = 2.75WRR155 pKa = 11.84FRR157 pKa = 11.84PVAEE161 pKa = 4.77SYY163 pKa = 9.27VHH165 pKa = 7.04PDD167 pKa = 2.85GLEE170 pKa = 3.96FEE172 pKa = 5.12LGMVPEE178 pKa = 4.1LVEE181 pKa = 3.82WRR183 pKa = 11.84EE184 pKa = 3.68QSLGHH189 pKa = 6.8DD190 pKa = 3.76LVIYY194 pKa = 10.59GPTKK198 pKa = 10.11VGKK201 pKa = 5.85TTWARR206 pKa = 11.84SLGNHH211 pKa = 5.76VYY213 pKa = 10.79FMGVMSGEE221 pKa = 3.93VALRR225 pKa = 11.84DD226 pKa = 3.63MHH228 pKa = 6.71DD229 pKa = 3.05ADD231 pKa = 4.02YY232 pKa = 11.52AVFDD236 pKa = 4.5DD237 pKa = 3.87MRR239 pKa = 11.84GGIEE243 pKa = 5.44FFPSWKK249 pKa = 9.8EE250 pKa = 3.7WLGSQAVVTVKK261 pKa = 10.51KK262 pKa = 10.57LYY264 pKa = 10.05RR265 pKa = 11.84DD266 pKa = 3.54PVQVKK271 pKa = 8.69WGKK274 pKa = 9.44PSIWLSNADD283 pKa = 3.52PRR285 pKa = 11.84SQLRR289 pKa = 11.84SSVHH293 pKa = 5.79HH294 pKa = 6.32ANEE297 pKa = 4.28GKK299 pKa = 9.66MSAIEE304 pKa = 4.9NDD306 pKa = 3.67IAWLEE311 pKa = 4.4GNCQFVYY318 pKa = 10.42IEE320 pKa = 4.67DD321 pKa = 4.42SIISHH326 pKa = 6.99ANTDD330 pKa = 3.26

Molecular weight:
37.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A220IGP9|A0A220IGP9_9VIRU Replication-associated protein OS=Giant panda associated gemycircularvirus OX=2016461 PE=3 SV=1
MM1 pKa = 7.71AYY3 pKa = 10.1SRR5 pKa = 11.84YY6 pKa = 9.32RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 8.09TRR11 pKa = 11.84RR12 pKa = 11.84TYY14 pKa = 10.53RR15 pKa = 11.84KK16 pKa = 7.54STRR19 pKa = 11.84GARR22 pKa = 11.84PSARR26 pKa = 11.84RR27 pKa = 11.84SRR29 pKa = 11.84YY30 pKa = 5.71TVKK33 pKa = 9.59TRR35 pKa = 11.84RR36 pKa = 11.84YY37 pKa = 6.47TRR39 pKa = 11.84KK40 pKa = 9.29RR41 pKa = 11.84PMSKK45 pKa = 9.87RR46 pKa = 11.84SVLNATSRR54 pKa = 11.84KK55 pKa = 9.68KK56 pKa = 10.33RR57 pKa = 11.84DD58 pKa = 3.23TMLSYY63 pKa = 11.7SNVTAAAPSGGTTFSTNPSILVGNQQYY90 pKa = 10.67ILPWVCTARR99 pKa = 11.84DD100 pKa = 3.58LSNASGGANIIINRR114 pKa = 11.84SEE116 pKa = 4.0RR117 pKa = 11.84TSTVCYY123 pKa = 9.8MRR125 pKa = 11.84GLAEE129 pKa = 4.64NIRR132 pKa = 11.84IQTTNGMPWMWRR144 pKa = 11.84RR145 pKa = 11.84VCFTLKK151 pKa = 10.54GDD153 pKa = 3.92YY154 pKa = 10.68LFNLSTSTALLFNEE168 pKa = 4.49TAPGGMRR175 pKa = 11.84RR176 pKa = 11.84SVTNWAGIGSLPEE189 pKa = 4.35LIFRR193 pKa = 11.84GTANVDD199 pKa = 2.77WNDD202 pKa = 3.74YY203 pKa = 10.46YY204 pKa = 11.33SAPLDD209 pKa = 3.47TSRR212 pKa = 11.84IKK214 pKa = 11.16VMYY217 pKa = 10.42DD218 pKa = 2.95RR219 pKa = 11.84VRR221 pKa = 11.84TIAAGNEE228 pKa = 4.09EE229 pKa = 4.81GCTRR233 pKa = 11.84TYY235 pKa = 10.33KK236 pKa = 9.75QWHH239 pKa = 6.16SMNKK243 pKa = 9.08NLVYY247 pKa = 10.6DD248 pKa = 3.9DD249 pKa = 4.35EE250 pKa = 4.45EE251 pKa = 6.09RR252 pKa = 11.84GDD254 pKa = 4.04GEE256 pKa = 4.08VGANFSTQGRR266 pKa = 11.84QGMGDD271 pKa = 3.85YY272 pKa = 10.83YY273 pKa = 11.63VVDD276 pKa = 4.44FFLARR281 pKa = 11.84TGSTSSDD288 pKa = 2.94QLSFNPEE295 pKa = 3.35ASLYY299 pKa = 7.89WHH301 pKa = 7.04EE302 pKa = 4.32KK303 pKa = 9.19

Molecular weight:
34.51 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

829

196

330

276.3

31.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.634 ± 0.819

0.965 ± 0.169

5.066 ± 0.965

5.549 ± 0.974

3.619 ± 0.613

8.323 ± 0.685

2.533 ± 0.835

3.981 ± 0.165

3.498 ± 0.434

8.323 ± 1.64

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.136 ± 0.554

4.463 ± 0.813

3.86 ± 0.35

3.257 ± 0.57

7.841 ± 1.632

10.615 ± 2.285

6.152 ± 1.92

5.911 ± 0.828

2.895 ± 0.376

3.378 ± 1.062

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski