Giant panda associated gemycircularvirus
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGP9|A0A220IGP9_9VIRU Replication-associated protein OS=Giant panda associated gemycircularvirus OX=2016461 PE=3 SV=1
MM1 pKa = 7.41 SFAFNSRR8 pKa = 11.84 YY9 pKa = 10.22 VLLTYY14 pKa = 10.11 AQSDD18 pKa = 4.16 GLSEE22 pKa = 4.04 WDD24 pKa = 3.58 VLEE27 pKa = 5.2 HH28 pKa = 6.83 ISGLGAEE35 pKa = 4.78 CIIARR40 pKa = 11.84 EE41 pKa = 3.98 NHH43 pKa = 6.51 ADD45 pKa = 3.66 GGTHH49 pKa = 6.67 LHH51 pKa = 6.68 AFCDD55 pKa = 4.96 FGRR58 pKa = 11.84 KK59 pKa = 8.2 FRR61 pKa = 11.84 SRR63 pKa = 11.84 RR64 pKa = 11.84 SDD66 pKa = 2.88 IFDD69 pKa = 3.12 VGGRR73 pKa = 11.84 HH74 pKa = 6.6 ANITPSRR81 pKa = 11.84 GRR83 pKa = 11.84 PEE85 pKa = 4.69 FGWDD89 pKa = 3.35 YY90 pKa = 11.26 AVKK93 pKa = 10.74 DD94 pKa = 3.75 GDD96 pKa = 4.06 VVAGGLARR104 pKa = 11.84 PGSGGLPEE112 pKa = 5.6 APNKK116 pKa = 8.8 WGQIVGAEE124 pKa = 4.02 NEE126 pKa = 4.12 QQFWEE131 pKa = 4.44 LVEE134 pKa = 4.34 EE135 pKa = 4.79 LDD137 pKa = 4.03 PKK139 pKa = 11.1 ALATQYY145 pKa = 10.76 PSLRR149 pKa = 11.84 KK150 pKa = 8.97 FADD153 pKa = 2.75 WRR155 pKa = 11.84 FRR157 pKa = 11.84 PVAEE161 pKa = 4.77 SYY163 pKa = 9.27 VHH165 pKa = 7.04 PDD167 pKa = 2.85 GLEE170 pKa = 3.96 FEE172 pKa = 5.12 LGMVPEE178 pKa = 4.1 LVEE181 pKa = 3.82 WRR183 pKa = 11.84 EE184 pKa = 3.68 QSLGHH189 pKa = 6.8 DD190 pKa = 3.76 LVIYY194 pKa = 10.59 GPTKK198 pKa = 10.11 VGKK201 pKa = 5.85 TTWARR206 pKa = 11.84 SLGNHH211 pKa = 5.76 VYY213 pKa = 10.79 FMGVMSGEE221 pKa = 3.93 VALRR225 pKa = 11.84 DD226 pKa = 3.63 MHH228 pKa = 6.71 DD229 pKa = 3.05 ADD231 pKa = 4.02 YY232 pKa = 11.52 AVFDD236 pKa = 4.5 DD237 pKa = 3.87 MRR239 pKa = 11.84 GGIEE243 pKa = 5.44 FFPSWKK249 pKa = 9.8 EE250 pKa = 3.7 WLGSQAVVTVKK261 pKa = 10.51 KK262 pKa = 10.57 LYY264 pKa = 10.05 RR265 pKa = 11.84 DD266 pKa = 3.54 PVQVKK271 pKa = 8.69 WGKK274 pKa = 9.44 PSIWLSNADD283 pKa = 3.52 PRR285 pKa = 11.84 SQLRR289 pKa = 11.84 SSVHH293 pKa = 5.79 HH294 pKa = 6.32 ANEE297 pKa = 4.28 GKK299 pKa = 9.66 MSAIEE304 pKa = 4.9 NDD306 pKa = 3.67 IAWLEE311 pKa = 4.4 GNCQFVYY318 pKa = 10.42 IEE320 pKa = 4.67 DD321 pKa = 4.42 SIISHH326 pKa = 6.99 ANTDD330 pKa = 3.26
Molecular weight: 37.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.182
IPC2_protein 5.169
IPC_protein 5.143
Toseland 5.232
ProMoST 5.27
Dawson 5.219
Bjellqvist 5.321
Wikipedia 5.118
Rodwell 5.143
Grimsley 5.219
Solomon 5.207
Lehninger 5.181
Nozaki 5.372
DTASelect 5.537
Thurlkill 5.283
EMBOSS 5.245
Sillero 5.448
Patrickios 4.088
IPC_peptide 5.219
IPC2_peptide 5.448
IPC2.peptide.svr19 5.385
Protein with the highest isoelectric point:
>tr|A0A220IGP9|A0A220IGP9_9VIRU Replication-associated protein OS=Giant panda associated gemycircularvirus OX=2016461 PE=3 SV=1
MM1 pKa = 7.71 AYY3 pKa = 10.1 SRR5 pKa = 11.84 YY6 pKa = 9.32 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 8.09 TRR11 pKa = 11.84 RR12 pKa = 11.84 TYY14 pKa = 10.53 RR15 pKa = 11.84 KK16 pKa = 7.54 STRR19 pKa = 11.84 GARR22 pKa = 11.84 PSARR26 pKa = 11.84 RR27 pKa = 11.84 SRR29 pKa = 11.84 YY30 pKa = 5.71 TVKK33 pKa = 9.59 TRR35 pKa = 11.84 RR36 pKa = 11.84 YY37 pKa = 6.47 TRR39 pKa = 11.84 KK40 pKa = 9.29 RR41 pKa = 11.84 PMSKK45 pKa = 9.87 RR46 pKa = 11.84 SVLNATSRR54 pKa = 11.84 KK55 pKa = 9.68 KK56 pKa = 10.33 RR57 pKa = 11.84 DD58 pKa = 3.23 TMLSYY63 pKa = 11.7 SNVTAAAPSGGTTFSTNPSILVGNQQYY90 pKa = 10.67 ILPWVCTARR99 pKa = 11.84 DD100 pKa = 3.58 LSNASGGANIIINRR114 pKa = 11.84 SEE116 pKa = 4.0 RR117 pKa = 11.84 TSTVCYY123 pKa = 9.8 MRR125 pKa = 11.84 GLAEE129 pKa = 4.64 NIRR132 pKa = 11.84 IQTTNGMPWMWRR144 pKa = 11.84 RR145 pKa = 11.84 VCFTLKK151 pKa = 10.54 GDD153 pKa = 3.92 YY154 pKa = 10.68 LFNLSTSTALLFNEE168 pKa = 4.49 TAPGGMRR175 pKa = 11.84 RR176 pKa = 11.84 SVTNWAGIGSLPEE189 pKa = 4.35 LIFRR193 pKa = 11.84 GTANVDD199 pKa = 2.77 WNDD202 pKa = 3.74 YY203 pKa = 10.46 YY204 pKa = 11.33 SAPLDD209 pKa = 3.47 TSRR212 pKa = 11.84 IKK214 pKa = 11.16 VMYY217 pKa = 10.42 DD218 pKa = 2.95 RR219 pKa = 11.84 VRR221 pKa = 11.84 TIAAGNEE228 pKa = 4.09 EE229 pKa = 4.81 GCTRR233 pKa = 11.84 TYY235 pKa = 10.33 KK236 pKa = 9.75 QWHH239 pKa = 6.16 SMNKK243 pKa = 9.08 NLVYY247 pKa = 10.6 DD248 pKa = 3.9 DD249 pKa = 4.35 EE250 pKa = 4.45 EE251 pKa = 6.09 RR252 pKa = 11.84 GDD254 pKa = 4.04 GEE256 pKa = 4.08 VGANFSTQGRR266 pKa = 11.84 QGMGDD271 pKa = 3.85 YY272 pKa = 10.83 YY273 pKa = 11.63 VVDD276 pKa = 4.44 FFLARR281 pKa = 11.84 TGSTSSDD288 pKa = 2.94 QLSFNPEE295 pKa = 3.35 ASLYY299 pKa = 7.89 WHH301 pKa = 7.04 EE302 pKa = 4.32 KK303 pKa = 9.19
Molecular weight: 34.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.175
ProMoST 10.058
Dawson 10.438
Bjellqvist 10.189
Wikipedia 10.672
Rodwell 10.584
Grimsley 10.526
Solomon 10.482
Lehninger 10.423
Nozaki 10.189
DTASelect 10.189
Thurlkill 10.262
EMBOSS 10.599
Sillero 10.35
Patrickios 9.838
IPC_peptide 10.467
IPC2_peptide 9.238
IPC2.peptide.svr19 8.51
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
829
196
330
276.3
31.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.634 ± 0.819
0.965 ± 0.169
5.066 ± 0.965
5.549 ± 0.974
3.619 ± 0.613
8.323 ± 0.685
2.533 ± 0.835
3.981 ± 0.165
3.498 ± 0.434
8.323 ± 1.64
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.136 ± 0.554
4.463 ± 0.813
3.86 ± 0.35
3.257 ± 0.57
7.841 ± 1.632
10.615 ± 2.285
6.152 ± 1.92
5.911 ± 0.828
2.895 ± 0.376
3.378 ± 1.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here