Litoreibacter ponti
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3849 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T6BNR7|A0A2T6BNR7_9RHOB Uncharacterized protein DUF3576 OS=Litoreibacter ponti OX=1510457 GN=C8N43_2382 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.22 KK3 pKa = 10.39 VLFASTALVAFGGAAVADD21 pKa = 3.9 VALSGRR27 pKa = 11.84 AEE29 pKa = 3.88 MGIAQSSSTNSEE41 pKa = 3.68 VEE43 pKa = 4.0 MQFFTDD49 pKa = 2.95 IDD51 pKa = 3.9 VTFTMTGEE59 pKa = 4.2 TDD61 pKa = 2.91 NGLTFGASVDD71 pKa = 3.96 LDD73 pKa = 3.78 EE74 pKa = 6.9 GGDD77 pKa = 3.73 GSSASDD83 pKa = 4.57 DD84 pKa = 3.71 NTDD87 pKa = 4.59 DD88 pKa = 3.6 GGATIFISGGFGTVTMGDD106 pKa = 3.21 TDD108 pKa = 4.55 GAMDD112 pKa = 4.36 WALKK116 pKa = 10.46 DD117 pKa = 3.53 AGNVGNGGSIADD129 pKa = 5.18 DD130 pKa = 3.79 EE131 pKa = 4.79 TSHH134 pKa = 6.94 PGYY137 pKa = 10.47 LGAYY141 pKa = 8.77 HH142 pKa = 7.52 DD143 pKa = 4.72 GAAGQDD149 pKa = 3.8 GQILRR154 pKa = 11.84 WDD156 pKa = 3.82 YY157 pKa = 11.46 SSGAFGVAISFEE169 pKa = 4.11 QGDD172 pKa = 4.12 RR173 pKa = 11.84 DD174 pKa = 3.61 LGATTGVADD183 pKa = 3.84 EE184 pKa = 4.58 TDD186 pKa = 3.46 GFGLGFKK193 pKa = 10.31 YY194 pKa = 10.64 ALDD197 pKa = 3.88 LSGTTVNFGLGYY209 pKa = 7.92 QTIDD213 pKa = 3.65 GLPIDD218 pKa = 3.91 VDD220 pKa = 3.6 IVGVSVDD227 pKa = 3.25 AAFGNGLSAGIVYY240 pKa = 9.87 TDD242 pKa = 3.41 GDD244 pKa = 3.91 IFGVADD250 pKa = 3.69 SDD252 pKa = 4.4 HH253 pKa = 7.25 IGVGLGYY260 pKa = 7.21 TTGALTLHH268 pKa = 6.74 ANYY271 pKa = 9.92 GQYY274 pKa = 11.24 DD275 pKa = 3.77 FGDD278 pKa = 3.74 GTEE281 pKa = 3.91 ADD283 pKa = 4.13 GYY285 pKa = 9.3 GLSVAYY291 pKa = 9.97 DD292 pKa = 3.73 LGGGAVVHH300 pKa = 6.15 MGYY303 pKa = 11.27 GDD305 pKa = 4.17 GDD307 pKa = 4.03 STATAAAAPSAVTSGDD323 pKa = 3.08 RR324 pKa = 11.84 KK325 pKa = 10.59 SFSLGLGLSFF335 pKa = 5.45
Molecular weight: 33.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.503
IPC_protein 3.554
Toseland 3.312
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.554
Rodwell 3.376
Grimsley 3.21
Solomon 3.567
Lehninger 3.528
Nozaki 3.681
DTASelect 4.012
Thurlkill 3.376
EMBOSS 3.554
Sillero 3.681
Patrickios 0.871
IPC_peptide 3.554
IPC2_peptide 3.656
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|A0A2T6BER2|A0A2T6BER2_9RHOB Allophanate hydrolase OS=Litoreibacter ponti OX=1510457 GN=C8N43_3364 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 KK14 pKa = 8.83 HH15 pKa = 4.64 RR16 pKa = 11.84 HH17 pKa = 3.91 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.37 AGRR29 pKa = 11.84 KK30 pKa = 8.54 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.35 GRR40 pKa = 11.84 AKK42 pKa = 10.69 LSAA45 pKa = 3.92
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.647
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.369
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.132
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3849
0
3849
1189232
26
4227
309.0
33.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.077 ± 0.046
0.911 ± 0.013
6.226 ± 0.044
6.033 ± 0.039
3.9 ± 0.023
8.646 ± 0.046
2.016 ± 0.022
5.22 ± 0.029
3.39 ± 0.034
9.916 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.833 ± 0.021
2.634 ± 0.023
5.033 ± 0.032
3.184 ± 0.019
6.28 ± 0.043
5.261 ± 0.03
5.606 ± 0.046
7.217 ± 0.03
1.374 ± 0.02
2.243 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here