Salinibacterium hongtaonis
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2605 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1T384|A0A2U1T384_9MICO MaoC family dehydratase OS=Salinibacterium hongtaonis OX=2079791 GN=DF220_11145 PE=3 SV=1
MM1 pKa = 7.39 SRR3 pKa = 11.84 LRR5 pKa = 11.84 RR6 pKa = 11.84 SAVALVAIIPLLLSGCFLIGGEE28 pKa = 4.35 SRR30 pKa = 11.84 TTSTPTGEE38 pKa = 4.66 KK39 pKa = 10.13 VDD41 pKa = 4.62 GDD43 pKa = 3.92 LQPFYY48 pKa = 10.87 DD49 pKa = 4.98 QVVEE53 pKa = 4.2 WEE55 pKa = 4.23 SCGDD59 pKa = 3.77 DD60 pKa = 4.03 FQCATAMAPLDD71 pKa = 3.41 WDD73 pKa = 3.7 DD74 pKa = 4.54 PEE76 pKa = 5.85 RR77 pKa = 11.84 EE78 pKa = 4.32 SIEE81 pKa = 3.93 LALIRR86 pKa = 11.84 LTTRR90 pKa = 11.84 GTPMGSLLVNPGGPGGSGVDD110 pKa = 4.19 FVRR113 pKa = 11.84 EE114 pKa = 3.87 SWDD117 pKa = 3.5 YY118 pKa = 11.1 ATTAQLRR125 pKa = 11.84 DD126 pKa = 3.51 NYY128 pKa = 10.86 DD129 pKa = 3.12 LVGFDD134 pKa = 3.8 PRR136 pKa = 11.84 GVGASSAVSCYY147 pKa = 10.5 DD148 pKa = 4.78 DD149 pKa = 4.91 PDD151 pKa = 3.8 TYY153 pKa = 11.37 TDD155 pKa = 3.67 YY156 pKa = 11.29 LYY158 pKa = 10.38 DD159 pKa = 3.75 ITPGTPGSAEE169 pKa = 3.85 WLDD172 pKa = 4.37 AMDD175 pKa = 3.18 ATMAEE180 pKa = 4.69 FGADD184 pKa = 3.1 CLEE187 pKa = 4.23 FTGEE191 pKa = 4.01 LLGFVDD197 pKa = 4.4 TVSAARR203 pKa = 11.84 DD204 pKa = 3.5 LDD206 pKa = 3.97 LLRR209 pKa = 11.84 AILGDD214 pKa = 3.96 DD215 pKa = 3.26 KK216 pKa = 11.65 LNYY219 pKa = 9.89 LGSSYY224 pKa = 10.26 GTFLGATYY232 pKa = 11.2 ADD234 pKa = 5.52 LYY236 pKa = 9.1 PQNTGRR242 pKa = 11.84 LVLDD246 pKa = 4.16 GAIDD250 pKa = 3.72 PASTDD255 pKa = 3.95 FEE257 pKa = 4.62 VTAMQAQGFEE267 pKa = 4.4 SAMRR271 pKa = 11.84 AYY273 pKa = 10.62 LADD276 pKa = 4.14 CLTGANCPFTGTVEE290 pKa = 4.4 SGMKK294 pKa = 10.22 EE295 pKa = 3.47 MGAIFARR302 pKa = 11.84 LEE304 pKa = 4.07 ASPLRR309 pKa = 11.84 AEE311 pKa = 4.91 DD312 pKa = 3.61 GRR314 pKa = 11.84 LLGSQTMFTAVILPLYY330 pKa = 10.52 SADD333 pKa = 2.59 TWQYY337 pKa = 8.61 LTSLITDD344 pKa = 3.92 VKK346 pKa = 10.54 RR347 pKa = 11.84 GSAEE351 pKa = 3.8 IAFSLADD358 pKa = 4.32 LYY360 pKa = 11.34 NGRR363 pKa = 11.84 EE364 pKa = 4.04 PDD366 pKa = 3.24 GTFADD371 pKa = 3.87 NSSEE375 pKa = 3.94 AFTAINCLDD384 pKa = 3.85 YY385 pKa = 10.83 PSTSTRR391 pKa = 11.84 EE392 pKa = 4.01 TLPAEE397 pKa = 4.34 AAEE400 pKa = 4.36 LAALAPVFGPQMSWGGTSCDD420 pKa = 2.99 AWPFEE425 pKa = 4.32 ATRR428 pKa = 11.84 VRR430 pKa = 11.84 GPIAADD436 pKa = 4.19 GSAPILVVGTTNDD449 pKa = 3.6 PATPYY454 pKa = 10.0 AWSVALADD462 pKa = 3.79 QLQNGHH468 pKa = 6.31 LVSYY472 pKa = 10.42 DD473 pKa = 3.83 GEE475 pKa = 4.3 GHH477 pKa = 5.16 TAYY480 pKa = 10.61 NKK482 pKa = 10.43 SNQCIDD488 pKa = 3.58 DD489 pKa = 3.81 TVDD492 pKa = 3.24 AFFIDD497 pKa = 3.93 GTVPATDD504 pKa = 4.05 PLCC507 pKa = 4.35
Molecular weight: 54.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.973
Patrickios 1.163
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A2U1T2F2|A0A2U1T2F2_9MICO Maltose ABC transporter permease OS=Salinibacterium hongtaonis OX=2079791 GN=DF220_09710 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2605
0
2605
856357
24
9317
328.7
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.003 ± 0.083
0.483 ± 0.01
5.855 ± 0.041
5.723 ± 0.049
3.241 ± 0.037
8.752 ± 0.059
1.907 ± 0.022
5.145 ± 0.033
2.257 ± 0.036
9.997 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.927 ± 0.025
2.277 ± 0.029
5.104 ± 0.041
2.842 ± 0.026
6.619 ± 0.062
6.453 ± 0.045
6.142 ± 0.079
8.923 ± 0.042
1.369 ± 0.021
1.981 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here