Pseudogracilibacillus auburnensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pseudogracilibacillus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3VSS2|A0A2V3VSS2_9BACI Uncharacterized protein OS=Pseudogracilibacillus auburnensis OX=1494959 GN=DFR56_11340 PE=4 SV=1
MM1 pKa = 7.29MNKK4 pKa = 9.06KK5 pKa = 9.74HH6 pKa = 5.14KK7 pKa = 9.11TGWLILFMITSIGLLFGCSKK27 pKa = 11.0NEE29 pKa = 3.99QISTNDD35 pKa = 3.48PSDD38 pKa = 3.97DD39 pKa = 3.83SLDD42 pKa = 3.54IEE44 pKa = 5.16AIEE47 pKa = 5.17SNFDD51 pKa = 3.05MAAFEE56 pKa = 4.45EE57 pKa = 5.27KK58 pKa = 10.36YY59 pKa = 9.6PSYY62 pKa = 10.57YY63 pKa = 10.66VEE65 pKa = 4.91FEE67 pKa = 4.01TDD69 pKa = 3.29SGFEE73 pKa = 4.59SIFIMNYY80 pKa = 9.79DD81 pKa = 4.08PYY83 pKa = 11.4DD84 pKa = 4.05FDD86 pKa = 5.48HH87 pKa = 6.93LPEE90 pKa = 4.23YY91 pKa = 10.85QEE93 pKa = 4.01VVQYY97 pKa = 11.14GFVSLTIEE105 pKa = 4.2DD106 pKa = 3.1VVYY109 pKa = 10.66AVDD112 pKa = 3.7ILEE115 pKa = 4.72GDD117 pKa = 3.91DD118 pKa = 4.17FYY120 pKa = 11.71SAEE123 pKa = 4.06FDD125 pKa = 3.81AEE127 pKa = 4.23TGAVLSEE134 pKa = 4.23EE135 pKa = 4.83GDD137 pKa = 3.43WAPDD141 pKa = 3.68GEE143 pKa = 4.52DD144 pKa = 2.81LSKK147 pKa = 10.69FVEE150 pKa = 4.69EE151 pKa = 4.34KK152 pKa = 10.96LEE154 pKa = 3.86MMEE157 pKa = 4.07WIIVKK162 pKa = 10.56SNATVRR168 pKa = 3.54

Molecular weight: 19.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3VSK0|A0A2V3VSK0_9BACI Aminoglycoside 6-adenylyltransferase OS=Pseudogracilibacillus auburnensis OX=1494959 GN=DFR56_114106 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84KK13 pKa = 8.71KK14 pKa = 8.82IHH16 pKa = 5.49GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.35GRR39 pKa = 11.84NVLSAA44 pKa = 3.9

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4444

0

4444

1279137

29

2532

287.8

32.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.556 ± 0.041

0.69 ± 0.01

5.147 ± 0.038

7.57 ± 0.049

4.748 ± 0.037

6.586 ± 0.04

2.229 ± 0.019

8.815 ± 0.044

6.912 ± 0.033

9.608 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.018

4.695 ± 0.029

3.424 ± 0.023

3.559 ± 0.025

3.728 ± 0.027

5.789 ± 0.025

5.668 ± 0.027

6.916 ± 0.03

0.959 ± 0.013

3.532 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski