Mycobacterium phage Filuzino

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3LZZ0|A0A3G3LZZ0_9CAUD Uncharacterized protein OS=Mycobacterium phage Filuzino OX=2484209 GN=77 PE=4 SV=1
MM1 pKa = 7.97PDD3 pKa = 2.9VHH5 pKa = 8.39IGFTGTRR12 pKa = 11.84DD13 pKa = 3.09AMPQVRR19 pKa = 11.84RR20 pKa = 11.84PDD22 pKa = 3.7GDD24 pKa = 3.94DD25 pKa = 3.25PAMNASEE32 pKa = 5.57DD33 pKa = 3.63GLEE36 pKa = 4.36PLGEE40 pKa = 4.45APDD43 pKa = 4.44LGAPHH48 pKa = 6.8YY49 pKa = 10.26PMSAEE54 pKa = 4.24VTFHH58 pKa = 7.62RR59 pKa = 11.84ARR61 pKa = 11.84CTKK64 pKa = 10.5CGDD67 pKa = 3.08IEE69 pKa = 4.31TDD71 pKa = 3.37YY72 pKa = 11.76GDD74 pKa = 4.22FSAYY78 pKa = 10.11SDD80 pKa = 3.39PGGAINAVLAAADD93 pKa = 3.49WFGRR97 pKa = 11.84SAPTGEE103 pKa = 5.08LIDD106 pKa = 4.08FGGGRR111 pKa = 11.84MGQRR115 pKa = 11.84YY116 pKa = 7.81QLVEE120 pKa = 4.46LLCPDD125 pKa = 3.92CQRR128 pKa = 11.84CEE130 pKa = 3.95VCGTAKK136 pKa = 10.4AYY138 pKa = 8.58PINDD142 pKa = 3.59HH143 pKa = 6.44LVCEE147 pKa = 4.23DD148 pKa = 4.1HH149 pKa = 7.29EE150 pKa = 4.31DD151 pKa = 3.94HH152 pKa = 6.79EE153 pKa = 5.25FEE155 pKa = 4.96AAPP158 pKa = 3.95

Molecular weight: 17.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3LZ69|A0A3G3LZ69_9CAUD Uncharacterized protein OS=Mycobacterium phage Filuzino OX=2484209 GN=96 PE=4 SV=1
MM1 pKa = 7.8AGTAVLTPDD10 pKa = 5.37GIDD13 pKa = 3.45TLVTAAEE20 pKa = 4.08AASLCGVTTSTIYY33 pKa = 10.15VWVNRR38 pKa = 11.84GTLAPSGKK46 pKa = 9.45NRR48 pKa = 11.84LGHH51 pKa = 5.16NVYY54 pKa = 10.31RR55 pKa = 11.84VLDD58 pKa = 3.67VAKK61 pKa = 10.59AEE63 pKa = 4.07HH64 pKa = 6.08ATRR67 pKa = 11.84AKK69 pKa = 10.42ARR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 4.63RR74 pKa = 3.93

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

18993

30

1161

180.9

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.693 ± 0.491

1.232 ± 0.171

6.924 ± 0.228

5.644 ± 0.271

2.933 ± 0.186

8.972 ± 0.525

2.264 ± 0.178

4.259 ± 0.196

3.149 ± 0.19

7.387 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.145

3.312 ± 0.173

6.108 ± 0.2

3.306 ± 0.177

6.808 ± 0.329

5.944 ± 0.316

6.818 ± 0.23

7.129 ± 0.27

2.338 ± 0.16

2.548 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski