Butyrivibrio virus Ceridwen
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9SU30|A0A6B9SU30_9CAUD Putative antirepressor OS=Butyrivibrio virus Ceridwen OX=2696359 PE=4 SV=1
MM1 pKa = 7.77 ALTDD5 pKa = 3.76 EE6 pKa = 4.79 NSNGMVMPVAPMGGGNGFGNFGGDD30 pKa = 3.4 GWWILLLFLLIGGNGWGGFGGYY52 pKa = 10.32 GGGNEE57 pKa = 4.85 LYY59 pKa = 10.15 PWMNQSNQINDD70 pKa = 3.63 GFRR73 pKa = 11.84 DD74 pKa = 3.52 QMINSNVTEE83 pKa = 3.9 IRR85 pKa = 11.84 NGVTGLATQMCNGFAGVEE103 pKa = 3.84 QGANARR109 pKa = 11.84 QIANMQQNFANQTAVTGAISDD130 pKa = 4.36 LASQQASCCCEE141 pKa = 3.71 SRR143 pKa = 11.84 LATCQTQNIVQNEE156 pKa = 4.01 GNATRR161 pKa = 11.84 FADD164 pKa = 3.78 ANNTRR169 pKa = 11.84 DD170 pKa = 3.95 IIDD173 pKa = 3.54 SQTRR177 pKa = 11.84 GTQAILDD184 pKa = 4.03 KK185 pKa = 11.25 LCALEE190 pKa = 4.09 LDD192 pKa = 4.27 GVKK195 pKa = 10.65 GQLAQAQRR203 pKa = 11.84 EE204 pKa = 4.28 NIALQNQINMGALRR218 pKa = 11.84 EE219 pKa = 4.27 SQTAQNAFISQGFANEE235 pKa = 3.17 VDD237 pKa = 3.21 ALYY240 pKa = 11.1 NRR242 pKa = 11.84 LSNCPVPSTPVYY254 pKa = 10.48 GRR256 pKa = 11.84 TPIFTCNQTPNCGCGCGMNN275 pKa = 3.83
Molecular weight: 29.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.168
IPC2_protein 4.457
IPC_protein 4.368
Toseland 4.177
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.215
Rodwell 4.19
Grimsley 4.088
Solomon 4.317
Lehninger 4.266
Nozaki 4.431
DTASelect 4.609
Thurlkill 4.202
EMBOSS 4.228
Sillero 4.469
Patrickios 1.138
IPC_peptide 4.317
IPC2_peptide 4.457
IPC2.peptide.svr19 4.384
Protein with the highest isoelectric point:
>tr|A0A6B9SV56|A0A6B9SV56_9CAUD Putative XRE family transcriptional inhibitor OS=Butyrivibrio virus Ceridwen OX=2696359 PE=4 SV=1
MM1 pKa = 7.62 SNPLFNQYY9 pKa = 9.13 GQNPYY14 pKa = 10.77 KK15 pKa = 11.07 GMIEE19 pKa = 4.02 QFNKK23 pKa = 10.0 FAQQIQGNPQEE34 pKa = 4.4 QVQQLLNSGRR44 pKa = 11.84 VTQTQYY50 pKa = 11.32 NAAIQKK56 pKa = 10.28 ANAIRR61 pKa = 11.84 SFFGVV66 pKa = 3.18
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.144
IPC2_protein 9.385
IPC_protein 9.472
Toseland 9.692
ProMoST 9.516
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.204
Rodwell 10.218
Grimsley 10.087
Solomon 10.072
Lehninger 10.028
Nozaki 9.633
DTASelect 9.706
Thurlkill 9.794
EMBOSS 10.101
Sillero 9.882
Patrickios 7.556
IPC_peptide 10.058
IPC2_peptide 8.375
IPC2.peptide.svr19 8.246
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
12092
51
844
165.6
18.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.658 ± 0.414
1.522 ± 0.204
5.938 ± 0.277
8.402 ± 0.399
3.432 ± 0.155
6.781 ± 0.299
1.274 ± 0.124
6.517 ± 0.276
8.138 ± 0.433
7.013 ± 0.272
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.787 ± 0.218
5.45 ± 0.262
2.804 ± 0.199
3.655 ± 0.199
4.325 ± 0.305
5.657 ± 0.318
6.674 ± 0.315
5.946 ± 0.262
1.373 ± 0.091
4.656 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here