Acidovorax sp. HDW3
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2817 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8AFS6|A0A6G8AFS6_9BURK DUF488 family protein OS=Acidovorax sp. HDW3 OX=2714923 GN=G7045_05880 PE=4 SV=1
MM1 pKa = 6.34 STEE4 pKa = 3.89 YY5 pKa = 10.9 VIVVTGSIVSEE16 pKa = 4.18 YY17 pKa = 10.51 YY18 pKa = 10.07 PEE20 pKa = 4.39 LKK22 pKa = 10.31 RR23 pKa = 11.84 ILISVQKK30 pKa = 10.24 RR31 pKa = 11.84 SAPYY35 pKa = 9.72 VIEE38 pKa = 4.37 GMFAEE43 pKa = 4.88 FGEE46 pKa = 4.61 VADD49 pKa = 4.66 GLFSALLDD57 pKa = 3.61 DD58 pKa = 4.39 HH59 pKa = 7.12 LGLFFSLIEE68 pKa = 4.0 VSEE71 pKa = 4.51 TNGDD75 pKa = 4.16 FRR77 pKa = 11.84 WGWEE81 pKa = 4.35 GYY83 pKa = 10.33 GYY85 pKa = 11.07 AEE87 pKa = 5.39 SFLQDD92 pKa = 3.32 VLQLFDD98 pKa = 5.79 LFGLQNLKK106 pKa = 11.1 GEE108 pKa = 4.43 VYY110 pKa = 10.62 GDD112 pKa = 3.44 EE113 pKa = 4.3 EE114 pKa = 4.66 IYY116 pKa = 10.73 RR117 pKa = 11.84 CIVTADD123 pKa = 4.22 SIDD126 pKa = 3.61 CEE128 pKa = 4.39 YY129 pKa = 11.37 VEE131 pKa = 4.71 RR132 pKa = 5.85
Molecular weight: 15.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.851
IPC2_protein 4.05
IPC_protein 3.973
Toseland 3.795
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.808
Rodwell 3.808
Grimsley 3.719
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.19
Thurlkill 3.821
EMBOSS 3.834
Sillero 4.088
Patrickios 0.693
IPC_peptide 3.923
IPC2_peptide 4.075
IPC2.peptide.svr19 3.979
Protein with the highest isoelectric point:
>tr|A0A6G8AK44|A0A6G8AK44_9BURK Nucleotidyltransferase family protein OS=Acidovorax sp. HDW3 OX=2714923 GN=G7045_14295 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.4 TKK25 pKa = 10.4 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2817
0
2817
951781
32
2129
337.9
36.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.626 ± 0.074
0.974 ± 0.014
4.873 ± 0.034
5.106 ± 0.046
3.284 ± 0.032
8.045 ± 0.045
2.393 ± 0.022
4.074 ± 0.039
3.094 ± 0.052
11.559 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.279 ± 0.023
2.461 ± 0.03
5.332 ± 0.036
5.441 ± 0.052
6.676 ± 0.041
5.049 ± 0.031
4.838 ± 0.031
7.092 ± 0.041
1.569 ± 0.022
2.235 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here