Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanococcoides; Methanococcoides burtonii

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2242 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q12U04|Q12U04_METBU RNA-splicing ligase RtcB OS=Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) OX=259564 GN=rtcB PE=3 SV=1
MM1 pKa = 7.05QSKK4 pKa = 10.37SRR6 pKa = 11.84TKK8 pKa = 7.68MHH10 pKa = 6.62RR11 pKa = 11.84RR12 pKa = 11.84YY13 pKa = 10.38ASIGITIAVVIMMVLSGPVSAVTVGITGLDD43 pKa = 3.24GTTPTKK49 pKa = 10.63GDD51 pKa = 3.41SVTFDD56 pKa = 3.14VTATIEE62 pKa = 4.79DD63 pKa = 3.89PDD65 pKa = 4.33KK66 pKa = 11.18YY67 pKa = 11.45VPIDD71 pKa = 3.85NFSLDD76 pKa = 3.18ITGATTTEE84 pKa = 4.52VVFSTDD90 pKa = 2.98GTVLSGSGITVVAVTSPLSAEE111 pKa = 4.04YY112 pKa = 10.61GHH114 pKa = 6.97GYY116 pKa = 10.15GYY118 pKa = 10.94GYY120 pKa = 10.45DD121 pKa = 3.65SNVGYY126 pKa = 10.42GYY128 pKa = 10.86DD129 pKa = 3.11FGYY132 pKa = 10.89GYY134 pKa = 11.07GYY136 pKa = 10.83GYY138 pKa = 11.0GYY140 pKa = 10.57GAGGEE145 pKa = 4.14DD146 pKa = 3.48VEE148 pKa = 4.56YY149 pKa = 10.48KK150 pKa = 9.46YY151 pKa = 9.43TITLDD156 pKa = 3.44TSILNTGAHH165 pKa = 5.61EE166 pKa = 4.65AVLSLNTGNSAKK178 pKa = 10.24PSFDD182 pKa = 3.56SPSASFTIEE191 pKa = 3.87AASSPSSGGSSGGGSGSGGVRR212 pKa = 11.84IVAADD217 pKa = 3.59DD218 pKa = 3.76TEE220 pKa = 4.28EE221 pKa = 5.07PEE223 pKa = 5.2DD224 pKa = 3.94DD225 pKa = 3.94TGSGDD230 pKa = 3.81GTDD233 pKa = 3.73GTTDD237 pKa = 3.51SDD239 pKa = 4.36DD240 pKa = 4.06GTDD243 pKa = 3.48GTTDD247 pKa = 3.3SDD249 pKa = 4.02DD250 pKa = 3.86GTNEE254 pKa = 4.01TTPEE258 pKa = 4.13TTEE261 pKa = 4.29KK262 pKa = 10.7PSGLLPGFEE271 pKa = 4.48AVFAIAGLLAVAYY284 pKa = 9.34IVRR287 pKa = 11.84RR288 pKa = 11.84KK289 pKa = 10.42DD290 pKa = 3.0IEE292 pKa = 3.86

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q12U96|Q12U96_METBU AAA ATPase OS=Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) OX=259564 GN=Mbur_2106 PE=3 SV=1
MM1 pKa = 7.6GKK3 pKa = 7.95TGSINWVKK11 pKa = 10.73VKK13 pKa = 10.17GRR15 pKa = 11.84KK16 pKa = 9.1GKK18 pKa = 9.95IIKK21 pKa = 8.14VQRR24 pKa = 11.84AFGAKK29 pKa = 9.27AHH31 pKa = 6.82PGPAQRR37 pKa = 11.84FSSSGAKK44 pKa = 9.49RR45 pKa = 11.84RR46 pKa = 11.84FLKK49 pKa = 10.45RR50 pKa = 11.84SPKK53 pKa = 10.59SIVNN57 pKa = 3.59

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2242

0

2242

676646

45

2552

301.8

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.797 ± 0.048

1.199 ± 0.022

6.339 ± 0.04

7.318 ± 0.048

4.013 ± 0.038

7.088 ± 0.048

1.935 ± 0.023

8.455 ± 0.057

6.406 ± 0.051

8.948 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.009 ± 0.028

4.593 ± 0.051

3.625 ± 0.027

2.432 ± 0.026

4.108 ± 0.04

6.749 ± 0.045

5.424 ± 0.043

7.318 ± 0.045

0.865 ± 0.018

3.379 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski