Firmicutes bacterium CAG:321

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7HIU3|R7HIU3_9FIRM Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase OS=Firmicutes bacterium CAG:321 OX=1263018 GN=lgt PE=3 SV=1
MM1 pKa = 7.15NLKK4 pKa = 10.16VNQDD8 pKa = 2.93KK9 pKa = 11.29CIGCGQCVSICEE21 pKa = 4.22DD22 pKa = 3.19VFNFNDD28 pKa = 3.26EE29 pKa = 4.02GLAEE33 pKa = 4.38VIEE36 pKa = 4.42TPIKK40 pKa = 10.59EE41 pKa = 4.45EE42 pKa = 3.97YY43 pKa = 10.94LEE45 pKa = 4.65DD46 pKa = 3.33ATNAKK51 pKa = 9.62EE52 pKa = 4.21SCPTEE57 pKa = 4.63AIEE60 pKa = 4.49EE61 pKa = 4.26AA62 pKa = 3.98

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7HJA9|R7HJA9_9FIRM Thioredoxin OS=Firmicutes bacterium CAG:321 OX=1263018 GN=BN608_00670 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84QEE5 pKa = 4.86DD6 pKa = 3.78LSKK9 pKa = 11.27VFDD12 pKa = 3.97RR13 pKa = 11.84TWTANHH19 pKa = 6.57NDD21 pKa = 2.96VAKK24 pKa = 10.5RR25 pKa = 11.84GRR27 pKa = 11.84EE28 pKa = 3.79RR29 pKa = 11.84EE30 pKa = 3.97RR31 pKa = 11.84KK32 pKa = 9.14FMNRR36 pKa = 11.84MFGRR40 pKa = 11.84KK41 pKa = 9.17DD42 pKa = 3.14NTEE45 pKa = 3.69ISHH48 pKa = 5.17TTNNNLTPGMRR59 pKa = 11.84AYY61 pKa = 10.91NLQKK65 pKa = 10.69NINGMGNFEE74 pKa = 4.05RR75 pKa = 11.84QNRR78 pKa = 11.84VNNIVKK84 pKa = 9.74KK85 pKa = 9.82WKK87 pKa = 10.16

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1220

0

1220

353202

30

1292

289.5

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.982 ± 0.063

0.991 ± 0.026

6.412 ± 0.06

7.047 ± 0.076

3.996 ± 0.054

5.364 ± 0.058

1.195 ± 0.027

10.634 ± 0.09

9.959 ± 0.071

8.83 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.032

7.978 ± 0.08

2.423 ± 0.034

1.633 ± 0.033

3.17 ± 0.046

6.335 ± 0.062

5.602 ± 0.071

6.048 ± 0.055

0.523 ± 0.019

5.456 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski