Dragonfly larvae associated circular virus-4
Average proteome isoelectric point is 8.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U2Q0|W5U2Q0_9VIRU Putative capsid protein OS=Dragonfly larvae associated circular virus-4 OX=1454025 PE=4 SV=1
MM1 pKa = 7.41 GLLRR5 pKa = 11.84 VYY7 pKa = 11.04 YY8 pKa = 10.14 SGQQARR14 pKa = 11.84 RR15 pKa = 11.84 LVILAVVWPMVGTTQRR31 pKa = 11.84 SRR33 pKa = 11.84 GWVFTINNYY42 pKa = 9.24 NEE44 pKa = 3.69 WDD46 pKa = 3.76 FVNISKK52 pKa = 10.88 LEE54 pKa = 3.95 EE55 pKa = 3.63 KK56 pKa = 9.9 AQYY59 pKa = 9.9 YY60 pKa = 10.08 IYY62 pKa = 10.5 GKK64 pKa = 10.18 EE65 pKa = 4.0 RR66 pKa = 11.84 GEE68 pKa = 4.16 EE69 pKa = 4.33 GTPHH73 pKa = 6.15 LQGFAYY79 pKa = 9.59 FKK81 pKa = 10.79 QRR83 pKa = 11.84 ISFNGIRR90 pKa = 11.84 DD91 pKa = 3.44 ILTRR95 pKa = 11.84 AHH97 pKa = 6.82 VEE99 pKa = 3.61 IQRR102 pKa = 11.84 GFNAQAIDD110 pKa = 3.65 YY111 pKa = 7.88 CKK113 pKa = 10.67 KK114 pKa = 10.3 EE115 pKa = 4.12 GEE117 pKa = 4.11 FTEE120 pKa = 4.03 WGEE123 pKa = 4.03 PSRR126 pKa = 11.84 GPAGQKK132 pKa = 10.41 DD133 pKa = 3.16 KK134 pKa = 11.08 WKK136 pKa = 10.84 DD137 pKa = 3.43 VLQLARR143 pKa = 11.84 QGKK146 pKa = 6.44 VQEE149 pKa = 3.97 IEE151 pKa = 3.71 EE152 pKa = 4.42 RR153 pKa = 11.84 YY154 pKa = 8.38 PAIFLRR160 pKa = 11.84 YY161 pKa = 8.2 FQKK164 pKa = 10.69 LCGFYY169 pKa = 10.42 RR170 pKa = 11.84 PEE172 pKa = 3.84 HH173 pKa = 6.33 SIILEE178 pKa = 4.05 NFTNEE183 pKa = 3.8 WWWGPTGTGKK193 pKa = 10.34 FKK195 pKa = 10.94 KK196 pKa = 10.75 LNDD199 pKa = 4.79 DD200 pKa = 3.67 YY201 pKa = 11.21 PDD203 pKa = 4.43 PYY205 pKa = 10.81 EE206 pKa = 5.29 KK207 pKa = 10.81 SLDD210 pKa = 3.83 PWWDD214 pKa = 2.9 NYY216 pKa = 8.62 QRR218 pKa = 11.84 EE219 pKa = 4.44 EE220 pKa = 3.77 IVAIEE225 pKa = 4.06 EE226 pKa = 4.0 FEE228 pKa = 4.24 PRR230 pKa = 11.84 CKK232 pKa = 10.28 INSFLLKK239 pKa = 9.96 RR240 pKa = 11.84 WADD243 pKa = 3.87 RR244 pKa = 11.84 YY245 pKa = 10.21 PFRR248 pKa = 11.84 CEE250 pKa = 3.5 VKK252 pKa = 10.51 GAFLSKK258 pKa = 10.34 LRR260 pKa = 11.84 PLKK263 pKa = 10.55 IIVISNYY270 pKa = 9.75 QLDD273 pKa = 3.75 EE274 pKa = 4.42 CFPNSKK280 pKa = 10.59 DD281 pKa = 3.48 LDD283 pKa = 3.79 PLKK286 pKa = 10.81 RR287 pKa = 11.84 RR288 pKa = 11.84 FKK290 pKa = 10.35 EE291 pKa = 3.4 IHH293 pKa = 6.05 FPP295 pKa = 3.97
Molecular weight: 35.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.026
IPC2_protein 8.141
IPC_protein 8.053
Toseland 8.317
ProMoST 8.565
Dawson 8.829
Bjellqvist 8.887
Wikipedia 8.902
Rodwell 8.916
Grimsley 8.375
Solomon 8.96
Lehninger 8.931
Nozaki 8.975
DTASelect 8.741
Thurlkill 8.799
EMBOSS 8.99
Sillero 9.033
Patrickios 4.444
IPC_peptide 8.946
IPC2_peptide 7.644
IPC2.peptide.svr19 7.726
Protein with the highest isoelectric point:
>tr|W5U2Q0|W5U2Q0_9VIRU Putative capsid protein OS=Dragonfly larvae associated circular virus-4 OX=1454025 PE=4 SV=1
MM1 pKa = 6.82 YY2 pKa = 10.24 GKK4 pKa = 10.17 RR5 pKa = 11.84 KK6 pKa = 7.12 YY7 pKa = 10.78 AKK9 pKa = 9.09 YY10 pKa = 10.37 ASAFAFRR17 pKa = 11.84 RR18 pKa = 11.84 NMRR21 pKa = 11.84 TRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 YY26 pKa = 8.61 GAPGDD31 pKa = 3.8 PSMTSIGNISPTGRR45 pKa = 11.84 SIARR49 pKa = 11.84 ANRR52 pKa = 11.84 SNIGRR57 pKa = 11.84 TLTGTTRR64 pKa = 11.84 SRR66 pKa = 11.84 YY67 pKa = 9.71 NSGQGVTQQHH77 pKa = 6.14 DD78 pKa = 3.32 VRR80 pKa = 11.84 LIYY83 pKa = 10.3 AKK85 pKa = 10.52 KK86 pKa = 10.43 RR87 pKa = 11.84 MPNYY91 pKa = 9.66 KK92 pKa = 9.7 KK93 pKa = 10.5 KK94 pKa = 8.51 PWRR97 pKa = 11.84 RR98 pKa = 11.84 FVKK101 pKa = 10.14 KK102 pKa = 8.76 VHH104 pKa = 5.98 AVSEE108 pKa = 4.21 KK109 pKa = 10.99 DD110 pKa = 3.22 LGSRR114 pKa = 11.84 TALFSNTITYY124 pKa = 10.77 NNTVDD129 pKa = 4.94 GADD132 pKa = 3.28 EE133 pKa = 4.42 CLTIGLYY140 pKa = 9.81 PLQSTDD146 pKa = 2.59 PWLNDD151 pKa = 3.15 LNTITSLEE159 pKa = 4.15 NQGNPTAAAGGTVDD173 pKa = 4.08 PTTKK177 pKa = 10.46 FIFQSGVLDD186 pKa = 3.41 LTIRR190 pKa = 11.84 NATTQTGQLNPDD202 pKa = 3.65 QFNAAATQEE211 pKa = 3.7 IDD213 pKa = 3.49 IYY215 pKa = 11.03 EE216 pKa = 4.19 IMCRR220 pKa = 11.84 ADD222 pKa = 3.84 FMQVSGGNKK231 pKa = 9.26 INLSQCFEE239 pKa = 4.37 EE240 pKa = 5.91 GSVITRR246 pKa = 11.84 NIANAPGDD254 pKa = 3.52 IKK256 pKa = 10.61 IANRR260 pKa = 11.84 GATPFDD266 pKa = 3.83 LPAALSRR273 pKa = 11.84 YY274 pKa = 8.13 GMKK277 pKa = 9.94 IIKK280 pKa = 7.49 KK281 pKa = 7.63 TKK283 pKa = 8.73 YY284 pKa = 9.06 FVPGGGTITYY294 pKa = 9.12 QIRR297 pKa = 11.84 DD298 pKa = 3.64 PKK300 pKa = 10.69 RR301 pKa = 11.84 RR302 pKa = 11.84 VSSFRR307 pKa = 11.84 DD308 pKa = 3.38 LNVEE312 pKa = 3.83 GGVNKK317 pKa = 10.2 VGWTKK322 pKa = 11.01 YY323 pKa = 10.64 LYY325 pKa = 10.33 INAKK329 pKa = 9.41 VIPGFLLGTGPASFVQRR346 pKa = 11.84 MQVGVTRR353 pKa = 11.84 KK354 pKa = 9.96 YY355 pKa = 8.0 MYY357 pKa = 10.27 KK358 pKa = 9.92 VEE360 pKa = 4.35 GFNEE364 pKa = 4.13 SRR366 pKa = 11.84 DD367 pKa = 3.74 SYY369 pKa = 10.82 STLNSTISAPAA380 pKa = 3.55
Molecular weight: 42.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.619
IPC_protein 9.897
Toseland 10.175
ProMoST 9.897
Dawson 10.394
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.745
Grimsley 10.467
Solomon 10.409
Lehninger 10.379
Nozaki 10.175
DTASelect 10.072
Thurlkill 10.233
EMBOSS 10.584
Sillero 10.292
Patrickios 10.292
IPC_peptide 10.409
IPC2_peptide 8.829
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
675
295
380
337.5
38.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.222 ± 1.137
1.185 ± 0.319
4.593 ± 0.096
5.333 ± 2.18
5.037 ± 0.879
8.0 ± 0.764
0.889 ± 0.293
6.667 ± 0.071
6.963 ± 0.522
6.37 ± 0.681
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.63 ± 0.596
5.778 ± 0.859
5.037 ± 0.242
4.444 ± 0.401
7.852 ± 0.035
5.481 ± 1.098
6.519 ± 2.172
4.889 ± 0.302
2.074 ± 1.036
5.037 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here