Cellvibrio sp. 79

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Cellvibrionaceae; Cellvibrio; unclassified Cellvibrio

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6FD15|A0A2T6FD15_9GAMM Uncharacterized protein OS=Cellvibrio sp. 79 OX=1954207 GN=B0W54_21465 PE=4 SV=1
HHH2 pKa = 7.74DD3 pKa = 4.36DDD5 pKa = 5.04DDD7 pKa = 4.25AGDDD11 pKa = 3.71CDDD14 pKa = 4.84DDD16 pKa = 4.48DDD18 pKa = 4.17DDD20 pKa = 4.34INNLTDDD27 pKa = 3.65CPLIANSNQLNTDDD41 pKa = 3.01DDD43 pKa = 4.47LGDDD47 pKa = 3.97CDDD50 pKa = 4.44DDD52 pKa = 3.47DDD54 pKa = 4.11DDD56 pKa = 4.14VLNAVDDD63 pKa = 4.01CPLNANPLQSDDD75 pKa = 3.27DDD77 pKa = 3.71KK78 pKa = 11.27DD79 pKa = 4.44IGDDD83 pKa = 3.59CDDD86 pKa = 3.15VEEE89 pKa = 5.12VACAPGKKK97 pKa = 10.37FEEE100 pKa = 5.04VLGSQTVATGLRR112 pKa = 11.84GVLCIGCGVLNPAYYY127 pKa = 9.05ASTINDDD134 pKa = 3.93ATLATPVAVIASVWGRR150 pKa = 11.84VDDD153 pKa = 4.47PTTYYY158 pKa = 9.85GSKKK162 pKa = 9.76VGFLVSLPVGLLDDD176 pKa = 4.89SLISGLKKK184 pKa = 7.8TTYYY188 pKa = 11.32NGVPQQASVASGLLSLQLLNLTGDDD213 pKa = 3.34TKKK216 pKa = 10.75LIYYY220 pKa = 10.53NTTSSFNQVEEE231 pKa = 4.1EEE233 pKa = 4.18KK234 pKa = 10.22AVVGLLSNLNVHHH247 pKa = 6.87LCVAPPPI

Molecular weight: 26.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6FHH4|A0A2T6FHH4_9GAMM TonB-dependent receptor OS=Cellvibrio sp. 79 OX=1954207 GN=B0W54_15430 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 9.95RR12 pKa = 11.84VRR14 pKa = 11.84NHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 9.06ALTQVV45 pKa = 2.92

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4688

0

4688

1645598

31

13137

351.0

38.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.304 ± 0.042

0.932 ± 0.012

5.454 ± 0.033

5.724 ± 0.038

4.126 ± 0.026

7.079 ± 0.048

2.13 ± 0.023

6.288 ± 0.032

4.832 ± 0.035

10.195 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.022

4.745 ± 0.044

4.239 ± 0.025

4.38 ± 0.03

4.912 ± 0.034

6.696 ± 0.05

5.635 ± 0.049

6.679 ± 0.038

1.423 ± 0.012

3.138 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski