Erwinia phage phiEa116

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Kolesnikvirus; unclassified Kolesnikvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1ZV06|H1ZV06_9CAUD Uncharacterized protein OS=Erwinia phage phiEa116 OX=1133366 GN=P11600280 PE=4 SV=1
MM1 pKa = 7.56SLFQQYY7 pKa = 9.93SPKK10 pKa = 10.78DD11 pKa = 3.9VVCSWNGIAITGFAPDD27 pKa = 3.18SFLRR31 pKa = 11.84LQRR34 pKa = 11.84TSALVTPVVGAGGDD48 pKa = 3.5VALTRR53 pKa = 11.84NADD56 pKa = 3.25KK57 pKa = 10.73TGTIEE62 pKa = 4.14IEE64 pKa = 4.15LMQTATSNLRR74 pKa = 11.84LAAIYY79 pKa = 10.55AEE81 pKa = 4.08QQLMEE86 pKa = 4.44LEE88 pKa = 4.59EE89 pKa = 4.8NISSNFVIYY98 pKa = 10.4DD99 pKa = 3.33PSGSVLATGRR109 pKa = 11.84NAWLQEE115 pKa = 4.0LPQVEE120 pKa = 4.88LGADD124 pKa = 3.65QNSKK128 pKa = 7.36TWTFGCEE135 pKa = 3.77NLDD138 pKa = 3.59YY139 pKa = 10.62TVTIPASGLTSVV151 pKa = 4.2

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1ZV35|H1ZV35_9CAUD Uncharacterized protein OS=Erwinia phage phiEa116 OX=1133366 GN=P11600570 PE=4 SV=1
MM1 pKa = 7.32NRR3 pKa = 11.84RR4 pKa = 11.84ITAVSKK10 pKa = 10.68DD11 pKa = 3.55FYY13 pKa = 10.02NRR15 pKa = 11.84RR16 pKa = 11.84MGEE19 pKa = 4.03LVKK22 pKa = 10.62QFPNVFNKK30 pKa = 10.07SLPMPLAIGVHH41 pKa = 5.16KK42 pKa = 9.27TLAEE46 pKa = 3.79QTEE49 pKa = 4.64FSRR52 pKa = 11.84KK53 pKa = 9.04EE54 pKa = 3.65INAMLSVWTRR64 pKa = 11.84RR65 pKa = 11.84HH66 pKa = 5.96EE67 pKa = 4.27YY68 pKa = 8.68VCAAVTHH75 pKa = 6.32GFRR78 pKa = 11.84CRR80 pKa = 11.84PEE82 pKa = 3.53RR83 pKa = 11.84RR84 pKa = 11.84VRR86 pKa = 3.47

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

14666

42

1157

252.9

28.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.271 ± 0.493

0.989 ± 0.138

6.307 ± 0.27

6.048 ± 0.325

3.743 ± 0.179

7.541 ± 0.36

1.705 ± 0.189

5.809 ± 0.136

6.532 ± 0.344

7.875 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.197

5.237 ± 0.264

3.368 ± 0.229

3.627 ± 0.191

4.364 ± 0.25

7.044 ± 0.318

7.003 ± 0.481

6.764 ± 0.259

1.261 ± 0.212

3.921 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski