Amino acid dipepetide frequency for Enterobacteria phage phi80 (Bacteriophage phi-80)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.543AlaAla: 11.543 ± 1.96
1.016AlaCys: 1.016 ± 0.262
6.098AlaAsp: 6.098 ± 0.644
7.55AlaGlu: 7.55 ± 1.185
2.977AlaPhe: 2.977 ± 0.513
7.696AlaGly: 7.696 ± 1.129
1.67AlaHis: 1.67 ± 0.313
5.808AlaIle: 5.808 ± 0.788
4.138AlaLys: 4.138 ± 0.455
6.97AlaLeu: 6.97 ± 0.68
3.92AlaMet: 3.92 ± 0.54
3.267AlaAsn: 3.267 ± 0.492
2.686AlaPro: 2.686 ± 0.472
5.372AlaGln: 5.372 ± 0.688
6.461AlaArg: 6.461 ± 0.995
6.098AlaSer: 6.098 ± 0.596
4.429AlaThr: 4.429 ± 0.66
6.316AlaVal: 6.316 ± 0.692
2.396AlaTrp: 2.396 ± 0.413
2.759AlaTyr: 2.759 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
1.307CysAla: 1.307 ± 0.3
0.218CysCys: 0.218 ± 0.128
1.089CysAsp: 1.089 ± 0.303
0.508CysGlu: 0.508 ± 0.181
0.436CysPhe: 0.436 ± 0.184
1.162CysGly: 1.162 ± 0.332
0.436CysHis: 0.436 ± 0.217
0.436CysIle: 0.436 ± 0.171
0.799CysLys: 0.799 ± 0.24
0.799CysLeu: 0.799 ± 0.217
0.218CysMet: 0.218 ± 0.133
0.508CysAsn: 0.508 ± 0.209
0.581CysPro: 0.581 ± 0.209
0.581CysGln: 0.581 ± 0.202
0.508CysArg: 0.508 ± 0.19
1.016CysSer: 1.016 ± 0.286
0.653CysThr: 0.653 ± 0.171
0.508CysVal: 0.508 ± 0.227
0.218CysTrp: 0.218 ± 0.104
0.29CysTyr: 0.29 ± 0.164
0.0CysXaa: 0.0 ± 0.0
Asp
5.881AspAla: 5.881 ± 0.61
0.653AspCys: 0.653 ± 0.21
3.92AspAsp: 3.92 ± 0.658
3.993AspGlu: 3.993 ± 0.535
2.033AspPhe: 2.033 ± 0.345
5.881AspGly: 5.881 ± 0.733
0.799AspHis: 0.799 ± 0.265
2.323AspIle: 2.323 ± 0.396
2.977AspLys: 2.977 ± 0.454
4.574AspLeu: 4.574 ± 0.559
1.888AspMet: 1.888 ± 0.383
2.759AspAsn: 2.759 ± 0.341
2.251AspPro: 2.251 ± 0.596
1.888AspGln: 1.888 ± 0.259
2.831AspArg: 2.831 ± 0.519
3.775AspSer: 3.775 ± 0.639
3.485AspThr: 3.485 ± 0.502
4.138AspVal: 4.138 ± 0.497
1.452AspTrp: 1.452 ± 0.27
2.251AspTyr: 2.251 ± 0.314
0.0AspXaa: 0.0 ± 0.0
Glu
6.026GluAla: 6.026 ± 1.102
0.944GluCys: 0.944 ± 0.29
2.178GluAsp: 2.178 ± 0.315
5.227GluGlu: 5.227 ± 0.906
1.888GluPhe: 1.888 ± 0.311
3.049GluGly: 3.049 ± 0.34
0.726GluHis: 0.726 ± 0.272
3.557GluIle: 3.557 ± 0.523
4.864GluLys: 4.864 ± 0.811
6.679GluLeu: 6.679 ± 0.71
2.178GluMet: 2.178 ± 0.383
2.178GluAsn: 2.178 ± 0.366
2.323GluPro: 2.323 ± 0.478
4.138GluGln: 4.138 ± 0.769
4.211GluArg: 4.211 ± 0.643
4.211GluSer: 4.211 ± 0.57
3.267GluThr: 3.267 ± 0.436
3.194GluVal: 3.194 ± 0.496
1.307GluTrp: 1.307 ± 0.291
2.033GluTyr: 2.033 ± 0.379
0.0GluXaa: 0.0 ± 0.0
Phe
2.251PheAla: 2.251 ± 0.385
0.581PheCys: 0.581 ± 0.185
2.396PheAsp: 2.396 ± 0.413
1.96PheGlu: 1.96 ± 0.329
1.089PhePhe: 1.089 ± 0.224
2.105PheGly: 2.105 ± 0.39
0.363PheHis: 0.363 ± 0.204
1.96PheIle: 1.96 ± 0.38
1.089PheLys: 1.089 ± 0.254
2.323PheLeu: 2.323 ± 0.369
0.944PheMet: 0.944 ± 0.23
1.234PheAsn: 1.234 ± 0.231
1.67PhePro: 1.67 ± 0.405
0.726PheGln: 0.726 ± 0.251
3.122PheArg: 3.122 ± 0.501
3.049PheSer: 3.049 ± 0.514
3.194PheThr: 3.194 ± 0.484
2.178PheVal: 2.178 ± 0.362
0.508PheTrp: 0.508 ± 0.176
0.871PheTyr: 0.871 ± 0.203
0.0PheXaa: 0.0 ± 0.0
Gly
6.607GlyAla: 6.607 ± 0.871
1.016GlyCys: 1.016 ± 0.251
3.703GlyAsp: 3.703 ± 0.436
3.557GlyGlu: 3.557 ± 0.504
2.468GlyPhe: 2.468 ± 0.419
5.953GlyGly: 5.953 ± 0.896
1.162GlyHis: 1.162 ± 0.285
5.082GlyIle: 5.082 ± 0.644
4.501GlyLys: 4.501 ± 0.602
5.518GlyLeu: 5.518 ± 0.596
2.541GlyMet: 2.541 ± 0.476
3.848GlyAsn: 3.848 ± 0.609
1.089GlyPro: 1.089 ± 0.248
2.614GlyGln: 2.614 ± 0.443
3.703GlyArg: 3.703 ± 0.548
4.719GlySer: 4.719 ± 0.699
4.138GlyThr: 4.138 ± 0.722
5.227GlyVal: 5.227 ± 0.712
1.525GlyTrp: 1.525 ± 0.347
1.888GlyTyr: 1.888 ± 0.359
0.0GlyXaa: 0.0 ± 0.0
His
1.162HisAla: 1.162 ± 0.292
0.145HisCys: 0.145 ± 0.1
0.944HisAsp: 0.944 ± 0.257
0.871HisGlu: 0.871 ± 0.284
0.799HisPhe: 0.799 ± 0.269
1.162HisGly: 1.162 ± 0.272
0.436HisHis: 0.436 ± 0.197
1.307HisIle: 1.307 ± 0.258
0.944HisLys: 0.944 ± 0.273
0.944HisLeu: 0.944 ± 0.308
0.29HisMet: 0.29 ± 0.144
1.016HisAsn: 1.016 ± 0.295
0.726HisPro: 0.726 ± 0.23
0.436HisGln: 0.436 ± 0.198
1.089HisArg: 1.089 ± 0.221
0.726HisSer: 0.726 ± 0.219
0.944HisThr: 0.944 ± 0.24
1.016HisVal: 1.016 ± 0.218
0.29HisTrp: 0.29 ± 0.17
0.508HisTyr: 0.508 ± 0.207
0.0HisXaa: 0.0 ± 0.0
Ile
4.864IleAla: 4.864 ± 0.6
1.089IleCys: 1.089 ± 0.296
4.501IleAsp: 4.501 ± 0.649
3.92IleGlu: 3.92 ± 0.718
1.96IlePhe: 1.96 ± 0.398
2.614IleGly: 2.614 ± 0.396
0.653IleHis: 0.653 ± 0.215
2.977IleIle: 2.977 ± 0.436
3.92IleLys: 3.92 ± 0.617
2.686IleLeu: 2.686 ± 0.426
1.089IleMet: 1.089 ± 0.374
3.703IleAsn: 3.703 ± 0.634
1.96IlePro: 1.96 ± 0.427
2.396IleGln: 2.396 ± 0.631
3.34IleArg: 3.34 ± 0.521
4.574IleSer: 4.574 ± 0.796
3.34IleThr: 3.34 ± 0.587
2.759IleVal: 2.759 ± 0.491
0.944IleTrp: 0.944 ± 0.294
1.089IleTyr: 1.089 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
4.792LysAla: 4.792 ± 0.689
0.726LysCys: 0.726 ± 0.335
3.194LysAsp: 3.194 ± 0.518
4.501LysGlu: 4.501 ± 0.66
1.96LysPhe: 1.96 ± 0.413
2.323LysGly: 2.323 ± 0.447
0.653LysHis: 0.653 ± 0.236
3.049LysIle: 3.049 ± 0.566
3.993LysLys: 3.993 ± 0.53
4.864LysLeu: 4.864 ± 0.528
1.888LysMet: 1.888 ± 0.331
1.888LysAsn: 1.888 ± 0.385
2.904LysPro: 2.904 ± 0.458
3.34LysGln: 3.34 ± 0.585
3.63LysArg: 3.63 ± 0.628
3.412LysSer: 3.412 ± 0.5
3.267LysThr: 3.267 ± 0.498
3.63LysVal: 3.63 ± 0.487
1.016LysTrp: 1.016 ± 0.255
1.815LysTyr: 1.815 ± 0.336
0.0LysXaa: 0.0 ± 0.0
Leu
7.986LeuAla: 7.986 ± 0.903
0.726LeuCys: 0.726 ± 0.253
5.3LeuAsp: 5.3 ± 0.589
3.63LeuGlu: 3.63 ± 0.509
2.614LeuPhe: 2.614 ± 0.479
4.356LeuGly: 4.356 ± 0.716
1.742LeuHis: 1.742 ± 0.41
3.92LeuIle: 3.92 ± 0.459
4.864LeuLys: 4.864 ± 0.653
4.574LeuLeu: 4.574 ± 0.514
1.96LeuMet: 1.96 ± 0.483
2.977LeuAsn: 2.977 ± 0.423
2.977LeuPro: 2.977 ± 0.497
3.34LeuGln: 3.34 ± 0.629
4.574LeuArg: 4.574 ± 0.58
6.534LeuSer: 6.534 ± 0.75
5.953LeuThr: 5.953 ± 0.589
4.646LeuVal: 4.646 ± 0.546
0.653LeuTrp: 0.653 ± 0.216
1.96LeuTyr: 1.96 ± 0.273
0.0LeuXaa: 0.0 ± 0.0
Met
3.485MetAla: 3.485 ± 0.426
0.29MetCys: 0.29 ± 0.147
0.944MetAsp: 0.944 ± 0.236
1.525MetGlu: 1.525 ± 0.384
0.726MetPhe: 0.726 ± 0.24
1.815MetGly: 1.815 ± 0.293
0.436MetHis: 0.436 ± 0.163
1.379MetIle: 1.379 ± 0.304
2.686MetLys: 2.686 ± 0.431
2.105MetLeu: 2.105 ± 0.373
0.944MetMet: 0.944 ± 0.301
1.307MetAsn: 1.307 ± 0.359
1.089MetPro: 1.089 ± 0.309
1.089MetGln: 1.089 ± 0.262
2.105MetArg: 2.105 ± 0.387
2.541MetSer: 2.541 ± 0.408
2.033MetThr: 2.033 ± 0.454
1.815MetVal: 1.815 ± 0.274
0.29MetTrp: 0.29 ± 0.13
0.581MetTyr: 0.581 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
4.574AsnAla: 4.574 ± 0.597
0.436AsnCys: 0.436 ± 0.201
2.323AsnAsp: 2.323 ± 0.427
2.323AsnGlu: 2.323 ± 0.41
1.307AsnPhe: 1.307 ± 0.299
4.937AsnGly: 4.937 ± 0.687
1.162AsnHis: 1.162 ± 0.266
2.541AsnIle: 2.541 ± 0.366
1.96AsnLys: 1.96 ± 0.385
2.614AsnLeu: 2.614 ± 0.37
0.799AsnMet: 0.799 ± 0.286
1.67AsnAsn: 1.67 ± 0.3
2.178AsnPro: 2.178 ± 0.328
1.67AsnGln: 1.67 ± 0.489
2.541AsnArg: 2.541 ± 0.491
2.541AsnSer: 2.541 ± 0.491
2.904AsnThr: 2.904 ± 0.61
2.178AsnVal: 2.178 ± 0.382
0.944AsnTrp: 0.944 ± 0.222
1.525AsnTyr: 1.525 ± 0.309
0.0AsnXaa: 0.0 ± 0.0
Pro
3.92ProAla: 3.92 ± 0.513
0.218ProCys: 0.218 ± 0.123
2.686ProAsp: 2.686 ± 0.447
2.396ProGlu: 2.396 ± 0.55
1.742ProPhe: 1.742 ± 0.381
3.412ProGly: 3.412 ± 0.533
0.653ProHis: 0.653 ± 0.188
1.307ProIle: 1.307 ± 0.267
2.614ProLys: 2.614 ± 0.41
2.904ProLeu: 2.904 ± 0.477
0.653ProMet: 0.653 ± 0.2
1.016ProAsn: 1.016 ± 0.328
1.67ProPro: 1.67 ± 0.366
1.67ProGln: 1.67 ± 0.306
1.307ProArg: 1.307 ± 0.255
2.541ProSer: 2.541 ± 0.445
1.742ProThr: 1.742 ± 0.5
4.066ProVal: 4.066 ± 0.556
0.653ProTrp: 0.653 ± 0.195
0.799ProTyr: 0.799 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
4.501GlnAla: 4.501 ± 1.061
0.799GlnCys: 0.799 ± 0.249
2.251GlnAsp: 2.251 ± 0.431
3.049GlnGlu: 3.049 ± 0.504
1.452GlnPhe: 1.452 ± 0.312
2.251GlnGly: 2.251 ± 0.37
0.871GlnHis: 0.871 ± 0.254
2.323GlnIle: 2.323 ± 0.324
2.541GlnLys: 2.541 ± 0.516
4.211GlnLeu: 4.211 ± 0.803
1.597GlnMet: 1.597 ± 0.425
1.888GlnAsn: 1.888 ± 0.369
1.379GlnPro: 1.379 ± 0.296
3.194GlnGln: 3.194 ± 0.829
3.049GlnArg: 3.049 ± 0.472
2.977GlnSer: 2.977 ± 0.57
2.541GlnThr: 2.541 ± 0.495
3.34GlnVal: 3.34 ± 0.403
0.871GlnTrp: 0.871 ± 0.224
1.597GlnTyr: 1.597 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
5.881ArgAla: 5.881 ± 0.75
0.436ArgCys: 0.436 ± 0.222
3.92ArgAsp: 3.92 ± 0.669
4.864ArgGlu: 4.864 ± 0.782
2.033ArgPhe: 2.033 ± 0.35
2.759ArgGly: 2.759 ± 0.422
0.799ArgHis: 0.799 ± 0.246
4.283ArgIle: 4.283 ± 0.555
3.703ArgLys: 3.703 ± 0.621
4.792ArgLeu: 4.792 ± 0.638
1.96ArgMet: 1.96 ± 0.377
2.904ArgAsn: 2.904 ± 0.504
1.597ArgPro: 1.597 ± 0.322
4.138ArgGln: 4.138 ± 0.877
6.098ArgArg: 6.098 ± 1.081
2.468ArgSer: 2.468 ± 0.497
2.831ArgThr: 2.831 ± 0.535
4.138ArgVal: 4.138 ± 0.579
0.944ArgTrp: 0.944 ± 0.314
1.742ArgTyr: 1.742 ± 0.372
0.0ArgXaa: 0.0 ± 0.0
Ser
7.26SerAla: 7.26 ± 0.91
0.581SerCys: 0.581 ± 0.251
4.864SerAsp: 4.864 ± 0.623
3.703SerGlu: 3.703 ± 0.415
2.686SerPhe: 2.686 ± 0.492
6.897SerGly: 6.897 ± 0.863
0.799SerHis: 0.799 ± 0.274
2.977SerIle: 2.977 ± 0.436
2.904SerLys: 2.904 ± 0.557
5.735SerLeu: 5.735 ± 0.671
2.105SerMet: 2.105 ± 0.352
2.759SerAsn: 2.759 ± 0.6
2.759SerPro: 2.759 ± 0.402
2.904SerGln: 2.904 ± 0.482
3.775SerArg: 3.775 ± 0.503
3.703SerSer: 3.703 ± 0.504
3.993SerThr: 3.993 ± 0.504
4.646SerVal: 4.646 ± 0.644
1.162SerTrp: 1.162 ± 0.25
1.96SerTyr: 1.96 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
6.607ThrAla: 6.607 ± 0.778
0.726ThrCys: 0.726 ± 0.23
3.34ThrAsp: 3.34 ± 0.586
3.557ThrGlu: 3.557 ± 0.563
1.96ThrPhe: 1.96 ± 0.383
5.155ThrGly: 5.155 ± 0.706
1.016ThrHis: 1.016 ± 0.262
2.977ThrIle: 2.977 ± 0.514
2.178ThrLys: 2.178 ± 0.355
5.009ThrLeu: 5.009 ± 0.667
1.525ThrMet: 1.525 ± 0.508
2.396ThrAsn: 2.396 ± 0.478
3.848ThrPro: 3.848 ± 0.545
2.468ThrGln: 2.468 ± 0.589
3.775ThrArg: 3.775 ± 0.511
3.703ThrSer: 3.703 ± 0.539
3.485ThrThr: 3.485 ± 0.637
4.138ThrVal: 4.138 ± 0.618
0.799ThrTrp: 0.799 ± 0.206
1.815ThrTyr: 1.815 ± 0.436
0.0ThrXaa: 0.0 ± 0.0
Val
5.808ValAla: 5.808 ± 0.555
0.871ValCys: 0.871 ± 0.287
3.267ValAsp: 3.267 ± 0.534
4.356ValGlu: 4.356 ± 0.582
2.251ValPhe: 2.251 ± 0.336
3.993ValGly: 3.993 ± 0.584
0.508ValHis: 0.508 ± 0.174
3.848ValIle: 3.848 ± 0.504
4.283ValLys: 4.283 ± 0.731
3.92ValLeu: 3.92 ± 0.509
1.815ValMet: 1.815 ± 0.395
3.485ValAsn: 3.485 ± 0.466
2.396ValPro: 2.396 ± 0.404
2.541ValGln: 2.541 ± 0.385
3.122ValArg: 3.122 ± 0.542
5.735ValSer: 5.735 ± 0.71
5.59ValThr: 5.59 ± 0.762
2.831ValVal: 2.831 ± 0.448
1.089ValTrp: 1.089 ± 0.33
1.888ValTyr: 1.888 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
1.597TrpAla: 1.597 ± 0.284
0.581TrpCys: 0.581 ± 0.214
1.089TrpAsp: 1.089 ± 0.227
1.016TrpGlu: 1.016 ± 0.264
0.436TrpPhe: 0.436 ± 0.183
1.089TrpGly: 1.089 ± 0.223
0.508TrpHis: 0.508 ± 0.18
1.016TrpIle: 1.016 ± 0.249
0.799TrpLys: 0.799 ± 0.237
1.67TrpLeu: 1.67 ± 0.397
0.29TrpMet: 0.29 ± 0.155
0.944TrpAsn: 0.944 ± 0.305
0.436TrpPro: 0.436 ± 0.164
1.016TrpGln: 1.016 ± 0.277
0.726TrpArg: 0.726 ± 0.223
1.089TrpSer: 1.089 ± 0.287
1.089TrpThr: 1.089 ± 0.289
1.379TrpVal: 1.379 ± 0.252
0.145TrpTrp: 0.145 ± 0.101
0.581TrpTyr: 0.581 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.122TyrAla: 3.122 ± 0.454
0.218TyrCys: 0.218 ± 0.109
1.742TyrAsp: 1.742 ± 0.344
1.67TyrGlu: 1.67 ± 0.309
0.799TyrPhe: 0.799 ± 0.199
2.323TyrGly: 2.323 ± 0.391
0.363TyrHis: 0.363 ± 0.18
1.307TyrIle: 1.307 ± 0.279
1.089TyrLys: 1.089 ± 0.229
2.396TyrLeu: 2.396 ± 0.513
0.508TyrMet: 0.508 ± 0.199
1.379TyrAsn: 1.379 ± 0.271
1.452TyrPro: 1.452 ± 0.291
1.162TyrGln: 1.162 ± 0.203
2.323TyrArg: 2.323 ± 0.369
2.759TyrSer: 2.759 ± 0.452
1.597TyrThr: 1.597 ± 0.367
1.452TyrVal: 1.452 ± 0.361
0.29TyrTrp: 0.29 ± 0.121
0.944TyrTyr: 0.944 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13775 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski