Enterococcus phage IME-EFm1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060ANL5|A0A060ANL5_9CAUD Uncharacterized protein OS=Enterococcus phage IME-EFm1 OX=1445858 GN=IME_056 PE=4 SV=1
MM1 pKa = 7.34TFMMMGLLFIGAVIYY16 pKa = 10.23GAGVLIQWLEE26 pKa = 3.85EE27 pKa = 3.75GGYY30 pKa = 10.51

Molecular weight: 3.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060ANM0|A0A060ANM0_9CAUD Uncharacterized protein OS=Enterococcus phage IME-EFm1 OX=1445858 GN=IME_061 PE=4 SV=1
MM1 pKa = 6.43TTFIILAKK9 pKa = 10.38RR10 pKa = 11.84NAQTIQLSYY19 pKa = 10.5LAEE22 pKa = 4.15TMRR25 pKa = 11.84GAIDD29 pKa = 3.98EE30 pKa = 4.45FRR32 pKa = 11.84LTYY35 pKa = 9.75PKK37 pKa = 10.27KK38 pKa = 9.32KK39 pKa = 10.68YY40 pKa = 10.4KK41 pKa = 10.45IIDD44 pKa = 3.67CKK46 pKa = 10.82EE47 pKa = 3.6ATNYY51 pKa = 9.55NRR53 pKa = 4.44

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12808

30

1074

183.0

20.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.645 ± 0.312

0.547 ± 0.103

5.879 ± 0.173

7.706 ± 0.501

3.654 ± 0.164

6.426 ± 0.582

1.546 ± 0.162

6.597 ± 0.227

8.823 ± 0.403

8.698 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.184

6.66 ± 0.3

2.998 ± 0.197

3.795 ± 0.193

3.771 ± 0.201

5.84 ± 0.384

6.566 ± 0.401

6.246 ± 0.285

1.273 ± 0.164

4.653 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski