Polaromonas sp. OV174

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Polaromonas; unclassified Polaromonas

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4300 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1EYI9|A0A1I1EYI9_9BURK Isocitrate dehydrogenase kinase/phosphatase OS=Polaromonas sp. OV174 OX=1855300 GN=aceK PE=3 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.41NIQTEE11 pKa = 4.42MPAPFVFTDD20 pKa = 3.63SAAAKK25 pKa = 9.27VAEE28 pKa = 5.01LIAEE32 pKa = 4.35EE33 pKa = 4.62GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.81EE60 pKa = 4.64ITNEE64 pKa = 4.77DD65 pKa = 4.2DD66 pKa = 3.25TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.46QYY85 pKa = 11.17LIGAEE90 pKa = 3.96IDD92 pKa = 3.77YY93 pKa = 11.19KK94 pKa = 11.39DD95 pKa = 4.47DD96 pKa = 4.08LEE98 pKa = 4.56GAQFVIKK105 pKa = 10.53NPNATSTCGCGSSFSAA121 pKa = 4.79

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1IJ31|A0A1I1IJ31_9BURK Thioredoxin OS=Polaromonas sp. OV174 OX=1855300 GN=SAMN05216344_11528 PE=3 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.63MKK5 pKa = 9.83TKK7 pKa = 10.72SSAKK11 pKa = 9.15KK12 pKa = 9.67RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.62RR24 pKa = 11.84GQAFKK29 pKa = 11.01RR30 pKa = 11.84HH31 pKa = 5.79ILTKK35 pKa = 9.78KK36 pKa = 3.98TTKK39 pKa = 10.23NKK41 pKa = 9.15RR42 pKa = 11.84QLRR45 pKa = 11.84GSTAVHH51 pKa = 5.31EE52 pKa = 4.64TNMGHH57 pKa = 6.22MAQMLPGRR65 pKa = 11.84GII67 pKa = 3.49

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4300

0

4300

1345509

39

2744

312.9

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.445 ± 0.05

0.97 ± 0.012

4.908 ± 0.024

5.175 ± 0.029

3.587 ± 0.024

8.081 ± 0.033

2.179 ± 0.019

4.63 ± 0.027

3.886 ± 0.032

11.054 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.017

2.803 ± 0.021

5.068 ± 0.024

4.309 ± 0.03

6.129 ± 0.032

5.896 ± 0.026

5.083 ± 0.021

7.46 ± 0.032

1.413 ± 0.017

2.283 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski