Arthrobacter psychrolactophilus
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3412 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V5IM65|A0A2V5IM65_9MICC N-acetyltransferase OS=Arthrobacter psychrolactophilus OX=92442 GN=CVS30_16880 PE=4 SV=1
MM1 pKa = 7.65 TDD3 pKa = 3.17 LRR5 pKa = 11.84 SVGMLVAVLTLGLTSCAADD24 pKa = 3.28 SASEE28 pKa = 4.14 VTAVTEE34 pKa = 4.02 EE35 pKa = 3.99 TAAFALLPPAGIPDD49 pKa = 3.77 YY50 pKa = 10.73 QLGGSYY56 pKa = 10.73 VPIADD61 pKa = 3.51 VEE63 pKa = 4.37 IVVRR67 pKa = 11.84 DD68 pKa = 3.82 RR69 pKa = 11.84 LSTPDD74 pKa = 3.35 PGRR77 pKa = 11.84 YY78 pKa = 8.94 SICYY82 pKa = 9.22 INGFQTQPAEE92 pKa = 4.14 LEE94 pKa = 4.03 LWPEE98 pKa = 3.89 NTLLTDD104 pKa = 3.66 EE105 pKa = 5.42 SGLVMDD111 pKa = 5.91 PDD113 pKa = 3.89 WPDD116 pKa = 3.0 EE117 pKa = 4.06 ALLDD121 pKa = 3.64 TSTNEE126 pKa = 3.57 KK127 pKa = 9.96 RR128 pKa = 11.84 VQILAVLKK136 pKa = 10.05 PWIQNCATAGYY147 pKa = 10.12 AAVEE151 pKa = 3.98 FDD153 pKa = 4.33 NLDD156 pKa = 3.29 SYY158 pKa = 11.42 TRR160 pKa = 11.84 TDD162 pKa = 3.37 GALTKK167 pKa = 10.53 ADD169 pKa = 3.96 NLEE172 pKa = 4.15 LATDD176 pKa = 4.23 LVAFTHH182 pKa = 6.44 AKK184 pKa = 9.81 GLAAGQKK191 pKa = 9.43 NAAEE195 pKa = 4.59 DD196 pKa = 3.58 AAEE199 pKa = 4.34 LQSQASFDD207 pKa = 3.77 FAVSEE212 pKa = 4.1 EE213 pKa = 4.01 CAAYY217 pKa = 10.02 DD218 pKa = 3.58 EE219 pKa = 5.15 CSLYY223 pKa = 10.37 TGVYY227 pKa = 8.66 RR228 pKa = 11.84 EE229 pKa = 3.82 QVIDD233 pKa = 4.75 IEE235 pKa = 4.6 YY236 pKa = 9.79 TDD238 pKa = 3.44 QLPRR242 pKa = 11.84 SFASMCADD250 pKa = 3.14 AGVPSSMILRR260 pKa = 11.84 DD261 pKa = 3.66 RR262 pKa = 11.84 DD263 pKa = 3.67 LVTPAEE269 pKa = 4.49 DD270 pKa = 4.48 GYY272 pKa = 11.37 HH273 pKa = 6.62 FEE275 pKa = 4.47 RR276 pKa = 11.84 CTT278 pKa = 3.35
Molecular weight: 30.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.91
IPC_protein 3.91
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.024
Patrickios 1.252
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|A0A2V5IR90|A0A2V5IR90_9MICC Rhamnulokinase OS=Arthrobacter psychrolactophilus OX=92442 GN=CVS30_06960 PE=4 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.44 KK7 pKa = 9.48 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.32 RR11 pKa = 11.84 MSKK14 pKa = 9.94 KK15 pKa = 9.5 KK16 pKa = 9.79 HH17 pKa = 5.45 RR18 pKa = 11.84 KK19 pKa = 6.15 QLRR22 pKa = 11.84 KK23 pKa = 7.92 TRR25 pKa = 11.84 HH26 pKa = 3.43 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3412
0
3412
1124869
32
2033
329.7
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.696 ± 0.058
0.599 ± 0.011
5.361 ± 0.037
5.602 ± 0.039
3.326 ± 0.026
8.582 ± 0.033
2.096 ± 0.018
4.842 ± 0.033
3.074 ± 0.03
10.409 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.188 ± 0.018
2.762 ± 0.022
5.11 ± 0.029
3.206 ± 0.024
5.769 ± 0.036
6.426 ± 0.034
6.155 ± 0.043
8.26 ± 0.036
1.462 ± 0.02
2.076 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here