Bacteroidales bacterium 6E
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3618 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K8R1Y1|A0A0K8R1Y1_9BACT Uncharacterized protein OS=Bacteroidales bacterium 6E OX=1688776 GN=BA6E_12540 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 5.24 ASSRR6 pKa = 11.84 NWAPASSYY14 pKa = 11.47 SEE16 pKa = 4.13 IIKK19 pKa = 10.43 QNTMNKK25 pKa = 9.3 FLLFVAMILSFGHH38 pKa = 6.5 TMAQDD43 pKa = 3.65 HH44 pKa = 6.58 FKK46 pKa = 10.83 VAYY49 pKa = 8.61 TEE51 pKa = 4.14 QPQTYY56 pKa = 8.16 MNIYY60 pKa = 9.33 VFSASVGGNNLGAGDD75 pKa = 4.85 EE76 pKa = 4.17 IAVFDD81 pKa = 3.87 GTICCGVYY89 pKa = 10.07 KK90 pKa = 10.8 VSGAFAGPVNIPAAKK105 pKa = 10.29 AEE107 pKa = 4.11 AAGTGYY113 pKa = 8.19 TAGNPIVVKK122 pKa = 10.45 IWDD125 pKa = 3.46 ASAGIEE131 pKa = 4.01 YY132 pKa = 10.6 SADD135 pKa = 2.66 ISFRR139 pKa = 11.84 ANSPNTVFTNNEE151 pKa = 3.85 SAYY154 pKa = 9.25 MDD156 pKa = 3.59 ISAKK160 pKa = 9.81 VAATIQLTANNKK172 pKa = 9.08 TYY174 pKa = 10.99 DD175 pKa = 3.45 GTTNADD181 pKa = 2.7 VGYY184 pKa = 10.09 SVVDD188 pKa = 4.1 GSLAGDD194 pKa = 3.23 ITVNVANGLFADD206 pKa = 4.1 KK207 pKa = 10.53 HH208 pKa = 6.22 AGSGKK213 pKa = 9.61 PVTADD218 pKa = 3.0 ITVTGADD225 pKa = 3.65 IEE227 pKa = 4.78 NFDD230 pKa = 3.82 LTIIEE235 pKa = 4.56 TTTATISKK243 pKa = 8.55 KK244 pKa = 7.49 TVEE247 pKa = 4.67 VINAVAQDD255 pKa = 3.37 KK256 pKa = 10.66 VYY258 pKa = 11.05 NGNAVAVVSGASLAGVITPDD278 pKa = 3.15 EE279 pKa = 4.5 VIISGGTSGTFAQSNVGNAISVTTAMALTGSGASNYY315 pKa = 7.55 EE316 pKa = 4.39 LKK318 pKa = 10.73 SQPALSASITKK329 pKa = 10.56 APLTVTAEE337 pKa = 4.45 NKK339 pKa = 10.24 SKK341 pKa = 9.65 TYY343 pKa = 10.51 DD344 pKa = 3.42 GQVYY348 pKa = 10.65 SGFTSKK354 pKa = 8.83 ITGFVGGEE362 pKa = 4.13 TQSVISGSVTFTGDD376 pKa = 3.33 AISAINAGSYY386 pKa = 8.31 TVSPVVAGLSATNYY400 pKa = 10.35 SFSTANGTLTVSKK413 pKa = 11.05 APLTVTANDD422 pKa = 3.23 QTKK425 pKa = 10.27 VYY427 pKa = 10.62 GSANPALTIAYY438 pKa = 9.56 SGFVNGEE445 pKa = 4.14 TASVLATAPTASTQINATTNAGTYY469 pKa = 10.31 ADD471 pKa = 5.58 AITISGGVDD480 pKa = 3.03 EE481 pKa = 5.75 NYY483 pKa = 10.48 SFSYY487 pKa = 10.21 VAGDD491 pKa = 3.4 FTVTKK496 pKa = 10.78 APLTVTANDD505 pKa = 3.23 QTKK508 pKa = 10.27 VYY510 pKa = 10.62 GSANPALTIAYY521 pKa = 9.9 SGFEE525 pKa = 4.06 NGEE528 pKa = 4.17 TAQVLATAPTATTQISATTSAGTYY552 pKa = 8.61 TDD554 pKa = 5.41 AITVSGGVDD563 pKa = 3.22 EE564 pKa = 5.56 NYY566 pKa = 10.34 SFSYY570 pKa = 10.17 IAGDD574 pKa = 3.55 FTVTKK579 pKa = 10.79 APLTVVANDD588 pKa = 3.37 QIKK591 pKa = 10.69 VYY593 pKa = 10.75 GSANPVLTFNYY604 pKa = 9.99 SGFVNGEE611 pKa = 4.1 TAPVLATAPSAATLINATTNAGTYY635 pKa = 9.8 VDD637 pKa = 5.29 AISVSGGVDD646 pKa = 3.13 EE647 pKa = 5.63 NYY649 pKa = 10.71 SFTYY653 pKa = 9.86 VAGDD657 pKa = 3.39 FTVTKK662 pKa = 8.81 ATPAIQVTGGSYY674 pKa = 10.92 VFDD677 pKa = 3.85 GTGKK681 pKa = 10.12 SATAFAYY688 pKa = 10.07 GVGGTSDD695 pKa = 3.69 VLSPAVTITYY705 pKa = 8.76 TGADD709 pKa = 3.49 GTIYY713 pKa = 10.55 GPSNIAPSASGSYY726 pKa = 7.17 QVKK729 pKa = 10.61 ADD731 pKa = 3.77 FVGNTNYY738 pKa = 10.65 LAISATASLIIANTVSTITVTGPNSYY764 pKa = 9.26 TYY766 pKa = 10.53 NGQAQGPAAAEE777 pKa = 4.3 VTGSTGAVTYY787 pKa = 9.64 IYY789 pKa = 10.37 TGIAGTNYY797 pKa = 9.64 GPSSTPPMNAGSYY810 pKa = 8.27 QVVATVAADD819 pKa = 3.38 ATYY822 pKa = 10.72 QGASSAAFVFAIQKK836 pKa = 10.61 ASLTITATSQGRR848 pKa = 11.84 VYY850 pKa = 10.99 GIDD853 pKa = 3.52 NPQLTLSFEE862 pKa = 4.4 GFVGGDD868 pKa = 3.63 DD869 pKa = 3.31 EE870 pKa = 4.79 TVIDD874 pKa = 4.61 EE875 pKa = 5.05 LPLASTDD882 pKa = 3.69 AVISSDD888 pKa = 2.8 AGAYY892 pKa = 9.57 IIMVSGGNDD901 pKa = 2.82 NNYY904 pKa = 9.69 QFIYY908 pKa = 10.51 KK909 pKa = 10.04 EE910 pKa = 4.16 GVLTIMKK917 pKa = 9.81 AQATIQLLNLEE928 pKa = 4.15 QAYY931 pKa = 8.8 TGKK934 pKa = 9.4 PLEE937 pKa = 4.32 VSYY940 pKa = 8.99 QTTPAGLLVLFQYY953 pKa = 10.94 DD954 pKa = 4.91 GINILPSEE962 pKa = 4.23 LGEE965 pKa = 4.21 YY966 pKa = 10.32 VVTAVVSDD974 pKa = 3.94 KK975 pKa = 10.9 NHH977 pKa = 6.37 EE978 pKa = 4.22 GSTTGIFRR986 pKa = 11.84 ILEE989 pKa = 4.43 DD990 pKa = 4.06 EE991 pKa = 5.22 DD992 pKa = 5.79 GDD994 pKa = 4.69 GIPDD998 pKa = 4.01 IYY1000 pKa = 11.46 DD1001 pKa = 3.8 LDD1003 pKa = 4.08 SDD1005 pKa = 4.03 NDD1007 pKa = 4.04 GVPDD1011 pKa = 3.87 YY1012 pKa = 11.17 LDD1014 pKa = 4.32 KK1015 pKa = 11.24 FPEE1018 pKa = 5.08 DD1019 pKa = 3.68 SNEE1022 pKa = 3.93 WADD1025 pKa = 4.0 SDD1027 pKa = 4.76 GDD1029 pKa = 4.44 GIGDD1033 pKa = 4.38 NADD1036 pKa = 3.52 TDD1038 pKa = 4.17 DD1039 pKa = 5.88 DD1040 pKa = 4.65 NDD1042 pKa = 4.17 GVDD1045 pKa = 4.48 DD1046 pKa = 5.02 VDD1048 pKa = 5.1 DD1049 pKa = 5.24 AFPNDD1054 pKa = 3.59 PDD1056 pKa = 3.27 EE1057 pKa = 4.83 WEE1059 pKa = 5.11 DD1060 pKa = 4.01 SDD1062 pKa = 5.78 GDD1064 pKa = 4.46 GIGNNADD1071 pKa = 3.71 SDD1073 pKa = 4.19 DD1074 pKa = 5.15 DD1075 pKa = 4.94 GDD1077 pKa = 4.27 GVADD1081 pKa = 4.34 EE1082 pKa = 5.82 DD1083 pKa = 5.45 DD1084 pKa = 4.81 AFPLNPDD1091 pKa = 2.87 EE1092 pKa = 4.8 WLDD1095 pKa = 3.79 SDD1097 pKa = 5.0 GDD1099 pKa = 4.46 GIGDD1103 pKa = 4.38 NADD1106 pKa = 3.52 TDD1108 pKa = 4.29 DD1109 pKa = 5.89 DD1110 pKa = 4.67 NDD1112 pKa = 4.07 GTPDD1116 pKa = 3.51 EE1117 pKa = 4.33 QDD1119 pKa = 3.69 AFPFDD1124 pKa = 4.55 PNEE1127 pKa = 4.47 DD1128 pKa = 3.65 TDD1130 pKa = 4.15 SDD1132 pKa = 4.2 GDD1134 pKa = 4.29 GVGDD1138 pKa = 4.15 NSDD1141 pKa = 4.89 LFPLDD1146 pKa = 4.36 PEE1148 pKa = 4.25 QSDD1151 pKa = 3.84 DD1152 pKa = 3.81 QVAPEE1157 pKa = 4.61 LDD1159 pKa = 3.81 VTQLTPLTLVCGIDD1173 pKa = 4.2 FEE1175 pKa = 4.61 EE1176 pKa = 5.57 QLIAWLDD1183 pKa = 3.64 SQAGVVATDD1192 pKa = 2.99 NHH1194 pKa = 6.34 QLDD1197 pKa = 4.27 AWSHH1201 pKa = 6.03 DD1202 pKa = 3.64 LDD1204 pKa = 3.94 IEE1206 pKa = 4.66 EE1207 pKa = 5.11 ILSDD1211 pKa = 4.18 LCNEE1215 pKa = 4.37 SKK1217 pKa = 9.94 TIPVEE1222 pKa = 4.23 FTVTDD1227 pKa = 3.53 YY1228 pKa = 11.58 SGNSSSFTATLVVVVNAAPEE1248 pKa = 4.38 VISPLEE1254 pKa = 4.08 DD1255 pKa = 2.74 VVMAVNATHH1264 pKa = 7.62 RR1265 pKa = 11.84 IFASPLPGEE1274 pKa = 4.5 MFGDD1278 pKa = 4.19 DD1279 pKa = 3.22 QDD1281 pKa = 3.49 QALEE1285 pKa = 3.97 VRR1287 pKa = 11.84 YY1288 pKa = 8.63 FALGSDD1294 pKa = 4.39 TLPSWAKK1301 pKa = 10.07 VVNDD1305 pKa = 3.77 TLVFSPAIADD1315 pKa = 3.61 TGCYY1319 pKa = 9.25 QLVVEE1324 pKa = 6.1 ARR1326 pKa = 11.84 DD1327 pKa = 2.97 IWQASVTDD1335 pKa = 3.93 TFQVCIRR1342 pKa = 11.84 ATLVNSDD1349 pKa = 4.06 PLTMNGHH1356 pKa = 7.01 GIIVYY1361 pKa = 7.95 PNPASDD1367 pKa = 4.24 FVRR1370 pKa = 11.84 IKK1372 pKa = 10.34 PGKK1375 pKa = 8.82 QPEE1378 pKa = 4.48 TQVEE1382 pKa = 4.51 VTLMNLSGQAVIHH1395 pKa = 5.55 RR1396 pKa = 11.84 QFWSDD1401 pKa = 3.46 EE1402 pKa = 3.91 PLEE1405 pKa = 5.49 IDD1407 pKa = 4.23 LTGQKK1412 pKa = 9.31 TGMYY1416 pKa = 9.59 VIRR1419 pKa = 11.84 ITFNNQTMYY1428 pKa = 10.75 NKK1430 pKa = 10.55 LMIEE1434 pKa = 4.11 RR1435 pKa = 11.84 RR1436 pKa = 3.69
Molecular weight: 149.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.668
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.834
Patrickios 0.68
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A0K8QX80|A0A0K8QX80_9BACT Uncharacterized protein OS=Bacteroidales bacterium 6E OX=1688776 GN=BA6E_1093 PE=4 SV=1
MM1 pKa = 7.27 YY2 pKa = 10.26 ISLPLYY8 pKa = 9.01 NDD10 pKa = 3.03 NFIFCFSALINFNTASKK27 pKa = 10.53 SSSKK31 pKa = 10.76 KK32 pKa = 10.23 FDD34 pKa = 3.34 ISPLGCTRR42 pKa = 11.84 KK43 pKa = 10.02 NDD45 pKa = 3.68 VLIGASFFLDD55 pKa = 4.54 DD56 pKa = 3.65 ILSIEE61 pKa = 4.2 EE62 pKa = 4.37 CNEE65 pKa = 3.34 GCHH68 pKa = 6.58 PFFCPRR74 pKa = 11.84 ITPVVHH80 pKa = 6.42 HH81 pKa = 6.92 RR82 pKa = 11.84 LHH84 pKa = 6.26 GLVFVVACRR93 pKa = 11.84 QPRR96 pKa = 11.84 KK97 pKa = 9.82 RR98 pKa = 11.84 GGGVMRR104 pKa = 11.84 RR105 pKa = 11.84 CYY107 pKa = 10.6 KK108 pKa = 10.21 RR109 pKa = 11.84 VSRR112 pKa = 11.84 SLARR116 pKa = 11.84 PGSKK120 pKa = 10.08 RR121 pKa = 11.84 IPTGLASLSDD131 pKa = 3.55 PTRR134 pKa = 11.84 CVGYY138 pKa = 10.38 KK139 pKa = 9.88 RR140 pKa = 11.84 VIPTGLVFILIQRR153 pKa = 11.84 IVLATNVSPARR164 pKa = 11.84 WRR166 pKa = 11.84 VRR168 pKa = 11.84 DD169 pKa = 3.75 RR170 pKa = 11.84 NEE172 pKa = 4.36 SQWDD176 pKa = 3.43 WLRR179 pKa = 11.84 SLIQRR184 pKa = 11.84 VALVTNEE191 pKa = 3.34 PPARR195 pKa = 11.84 HH196 pKa = 6.41 RR197 pKa = 11.84 YY198 pKa = 9.17 LSGRR202 pKa = 11.84 PGSKK206 pKa = 9.3 TNPDD210 pKa = 3.28 GVQVGSLRR218 pKa = 3.62
Molecular weight: 24.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 9.604
IPC_protein 10.452
Toseland 10.628
ProMoST 10.526
Dawson 10.73
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.891
Grimsley 10.789
Solomon 10.862
Lehninger 10.818
Nozaki 10.657
DTASelect 10.452
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.599
IPC_peptide 10.862
IPC2_peptide 9.823
IPC2.peptide.svr19 8.585
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3610
8
3618
1318582
10
5663
364.5
41.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.755 ± 0.035
0.797 ± 0.019
5.509 ± 0.034
6.435 ± 0.04
4.934 ± 0.027
7.216 ± 0.043
2.014 ± 0.021
7.263 ± 0.034
5.866 ± 0.038
9.216 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.696 ± 0.022
5.113 ± 0.033
4.168 ± 0.023
3.412 ± 0.021
4.749 ± 0.036
6.413 ± 0.033
5.563 ± 0.059
6.603 ± 0.036
1.37 ± 0.017
3.907 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here