Odonata-associated circular virus-1
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UGI4|A0A0B4UGI4_9VIRU Putative capsid protein OS=Odonata-associated circular virus-1 OX=1592109 PE=4 SV=1
MM1 pKa = 7.38 SAEE4 pKa = 4.01 QPKK7 pKa = 10.4 PSDD10 pKa = 3.81 KK11 pKa = 10.79 ASHH14 pKa = 6.58 WLLTLNNPTADD25 pKa = 3.52 DD26 pKa = 4.06 RR27 pKa = 11.84 EE28 pKa = 4.31 RR29 pKa = 11.84 CKK31 pKa = 10.78 NLPKK35 pKa = 9.44 WVKK38 pKa = 9.77 RR39 pKa = 11.84 FKK41 pKa = 10.77 FQDD44 pKa = 3.68 EE45 pKa = 4.23 IGEE48 pKa = 4.38 SGTLHH53 pKa = 5.11 IQGYY57 pKa = 8.48 IHH59 pKa = 6.94 TEE61 pKa = 4.1 TVRR64 pKa = 11.84 LSALKK69 pKa = 9.34 KK70 pKa = 8.18 WFPRR74 pKa = 11.84 GHH76 pKa = 6.48 FEE78 pKa = 4.11 VCRR81 pKa = 11.84 NPKK84 pKa = 9.82 AAEE87 pKa = 3.91 NYY89 pKa = 9.43 AGKK92 pKa = 10.51 EE93 pKa = 4.06 EE94 pKa = 4.26 TSVPGTQHH102 pKa = 6.84 DD103 pKa = 4.08 FKK105 pKa = 11.13 NTVEE109 pKa = 4.28 QVTMMDD115 pKa = 3.52 NLIRR119 pKa = 11.84 LCDD122 pKa = 3.86 IQPKK126 pKa = 10.27 IIDD129 pKa = 3.32 HH130 pKa = 7.5 LIRR133 pKa = 11.84 QNAVSTDD140 pKa = 3.1 AKK142 pKa = 10.62 QVLTGKK148 pKa = 9.51 QLYY151 pKa = 8.51 QEE153 pKa = 4.89 EE154 pKa = 4.88 YY155 pKa = 8.01 WGCVNVILEE164 pKa = 4.59 EE165 pKa = 4.24 NPDD168 pKa = 4.27 LISTYY173 pKa = 9.64 TMPQYY178 pKa = 10.84 CKK180 pKa = 10.26 AWVNCRR186 pKa = 11.84 PAILNAHH193 pKa = 6.63 RR194 pKa = 11.84 VRR196 pKa = 11.84 QYY198 pKa = 10.82 EE199 pKa = 3.97 NEE201 pKa = 4.12 RR202 pKa = 11.84 TAGQPDD208 pKa = 3.98 KK209 pKa = 11.71 NEE211 pKa = 4.46 IITPEE216 pKa = 4.11 DD217 pKa = 3.43 LTTNEE222 pKa = 4.79 CLICSKK228 pKa = 10.36 EE229 pKa = 4.02 KK230 pKa = 11.03 CEE232 pKa = 4.8 CEE234 pKa = 3.81 AA235 pKa = 4.99
Molecular weight: 27.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.676
IPC2_protein 5.741
IPC_protein 5.753
Toseland 6.008
ProMoST 6.084
Dawson 5.995
Bjellqvist 6.008
Wikipedia 5.982
Rodwell 5.982
Grimsley 6.097
Solomon 5.995
Lehninger 5.982
Nozaki 6.249
DTASelect 6.427
Thurlkill 6.427
EMBOSS 6.389
Sillero 6.351
Patrickios 4.024
IPC_peptide 6.008
IPC2_peptide 6.338
IPC2.peptide.svr19 6.324
Protein with the highest isoelectric point:
>tr|A0A0B4UGI4|A0A0B4UGI4_9VIRU Putative capsid protein OS=Odonata-associated circular virus-1 OX=1592109 PE=4 SV=1
MM1 pKa = 7.09 VKK3 pKa = 10.21 LVRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 VLRR12 pKa = 11.84 KK13 pKa = 9.33 RR14 pKa = 11.84 KK15 pKa = 9.07 SVPKK19 pKa = 9.47 RR20 pKa = 11.84 RR21 pKa = 11.84 MMRR24 pKa = 11.84 RR25 pKa = 11.84 GVGRR29 pKa = 11.84 ASVKK33 pKa = 9.56 RR34 pKa = 11.84 TSDD37 pKa = 3.29 YY38 pKa = 11.17 AKK40 pKa = 10.2 CVEE43 pKa = 4.04 IQEE46 pKa = 4.58 TKK48 pKa = 7.78 MTAVNDD54 pKa = 3.72 ATLDD58 pKa = 3.74 SVGGVINFCLQDD70 pKa = 3.24 FQRR73 pKa = 11.84 PQEE76 pKa = 3.76 IAHH79 pKa = 6.53 AYY81 pKa = 8.94 KK82 pKa = 10.43 YY83 pKa = 10.69 YY84 pKa = 10.56 RR85 pKa = 11.84 AAKK88 pKa = 10.24 CEE90 pKa = 3.96 ITFIPYY96 pKa = 10.09 FNIAQTANAAATQLPQLYY114 pKa = 7.46 MTVDD118 pKa = 3.28 RR119 pKa = 11.84 LSNRR123 pKa = 11.84 WIAPTEE129 pKa = 4.0 SEE131 pKa = 4.09 MLSRR135 pKa = 11.84 GVSPRR140 pKa = 11.84 LFTKK144 pKa = 10.43 KK145 pKa = 8.99 MRR147 pKa = 11.84 LSFKK151 pKa = 10.64 PNLLQGISLEE161 pKa = 4.44 TQQSVNYY168 pKa = 9.51 PSGSPPGGNPAGISNLGYY186 pKa = 10.09 QKK188 pKa = 10.96 AIAVFNKK195 pKa = 9.98 GLPTQQSYY203 pKa = 10.72 GYY205 pKa = 9.73 SNNSTTGALQAGQVLAPLGVNPYY228 pKa = 10.24 DD229 pKa = 3.6 IRR231 pKa = 11.84 YY232 pKa = 8.84 HH233 pKa = 5.83 GAAFVPAIEE242 pKa = 4.22 VLAANTPVAVGDD254 pKa = 3.81 IQVKK258 pKa = 8.22 ITWEE262 pKa = 4.19 SKK264 pKa = 9.99 RR265 pKa = 11.84 PRR267 pKa = 11.84 ALMTNSPLNEE277 pKa = 4.99 LIPYY281 pKa = 9.52 VSSCCQGNTNAVLNTQPTSYY301 pKa = 10.55 PSGAIGAAMTVLL313 pKa = 3.74
Molecular weight: 34.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.28
IPC2_protein 9.575
IPC_protein 9.838
Toseland 10.043
ProMoST 9.823
Dawson 10.292
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.599
Grimsley 10.379
Solomon 10.321
Lehninger 10.277
Nozaki 10.087
DTASelect 9.999
Thurlkill 10.131
EMBOSS 10.467
Sillero 10.204
Patrickios 9.926
IPC_peptide 10.306
IPC2_peptide 8.931
IPC2.peptide.svr19 8.507
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
548
235
313
274.0
30.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.212 ± 1.448
2.737 ± 0.975
3.467 ± 1.053
5.839 ± 2.262
2.372 ± 0.157
5.474 ± 1.056
1.642 ± 0.858
5.474 ± 0.31
6.204 ± 0.935
7.482 ± 0.159
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.43
6.204 ± 0.115
6.204 ± 0.432
5.657 ± 0.08
6.204 ± 0.705
5.839 ± 1.291
6.934 ± 0.192
6.569 ± 0.94
1.277 ± 0.546
3.832 ± 0.548
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here