Amino acid dipepetide frequency for Lates calcarifer (Barramundi) (Holocentrus calcarifer)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.891AlaAla: 5.891 ± 0.023	1.279AlaCys: 1.279 ± 0.007	3.141AlaAsp: 3.141 ± 0.01	4.498AlaGlu: 4.498 ± 0.016	2.434AlaPhe: 2.434 ± 0.009	4.17AlaGly: 4.17 ± 0.014	1.443AlaHis: 1.443 ± 0.007	2.91AlaIle: 2.91 ± 0.01	3.4AlaLys: 3.4 ± 0.013	6.521AlaLeu: 6.521 ± 0.017	1.637AlaMet: 1.637 ± 0.008	2.225AlaAsn: 2.225 ± 0.008	3.202AlaPro: 3.202 ± 0.013	2.859AlaGln: 2.859 ± 0.011	3.087AlaArg: 3.087 ± 0.009	5.05AlaSer: 5.05 ± 0.014	3.434AlaThr: 3.434 ± 0.01	4.926AlaVal: 4.926 ± 0.014	0.667AlaTrp: 0.667 ± 0.005	1.633AlaTyr: 1.633 ± 0.007	0.0AlaXaa: 0.0 ± 0.0
Cys	1.162CysAla: 1.162 ± 0.007	0.662CysCys: 0.662 ± 0.006	1.146CysAsp: 1.146 ± 0.009	1.255CysGlu: 1.255 ± 0.009	0.985CysPhe: 0.985 ± 0.005	1.505CysGly: 1.505 ± 0.009	0.671CysHis: 0.671 ± 0.005	1.091CysIle: 1.091 ± 0.008	1.143CysLys: 1.143 ± 0.007	2.283CysLeu: 2.283 ± 0.009	0.501CysMet: 0.501 ± 0.003	0.849CysAsn: 0.849 ± 0.007	1.257CysPro: 1.257 ± 0.009	1.062CysGln: 1.062 ± 0.007	1.274CysArg: 1.274 ± 0.008	2.1CysSer: 2.1 ± 0.011	1.217CysThr: 1.217 ± 0.006	1.702CysVal: 1.702 ± 0.009	0.307CysTrp: 0.307 ± 0.003	0.662CysTyr: 0.662 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	2.91AspAla: 2.91 ± 0.011	1.175AspCys: 1.175 ± 0.008	3.075AspAsp: 3.075 ± 0.014	3.684AspGlu: 3.684 ± 0.012	2.216AspPhe: 2.216 ± 0.009	3.646AspGly: 3.646 ± 0.015	1.224AspHis: 1.224 ± 0.007	2.908AspIle: 2.908 ± 0.011	2.824AspLys: 2.824 ± 0.009	5.079AspLeu: 5.079 ± 0.015	1.337AspMet: 1.337 ± 0.006	2.082AspAsn: 2.082 ± 0.01	2.861AspPro: 2.861 ± 0.01	1.973AspGln: 1.973 ± 0.008	2.764AspArg: 2.764 ± 0.012	4.318AspSer: 4.318 ± 0.014	2.688AspThr: 2.688 ± 0.008	3.315AspVal: 3.315 ± 0.012	0.696AspTrp: 0.696 ± 0.005	1.642AspTyr: 1.642 ± 0.008	0.0AspXaa: 0.0 ± 0.0
Glu	4.654GluAla: 4.654 ± 0.017	1.28GluCys: 1.28 ± 0.009	4.273GluAsp: 4.273 ± 0.012	7.416GluGlu: 7.416 ± 0.034	2.06GluPhe: 2.06 ± 0.009	4.136GluGly: 4.136 ± 0.014	1.444GluHis: 1.444 ± 0.008	3.019GluIle: 3.019 ± 0.009	4.615GluLys: 4.615 ± 0.021	6.265GluLeu: 6.265 ± 0.02	1.77GluMet: 1.77 ± 0.008	2.753GluAsn: 2.753 ± 0.009	2.621GluPro: 2.621 ± 0.012	3.047GluGln: 3.047 ± 0.013	4.2GluArg: 4.2 ± 0.019	4.082GluSer: 4.082 ± 0.011	3.383GluThr: 3.383 ± 0.013	4.402GluVal: 4.402 ± 0.015	0.729GluTrp: 0.729 ± 0.005	1.72GluTyr: 1.72 ± 0.007	0.0GluXaa: 0.0 ± 0.0
Phe	2.022PheAla: 2.022 ± 0.009	1.005PheCys: 1.005 ± 0.005	1.899PheAsp: 1.899 ± 0.007	1.96PheGlu: 1.96 ± 0.009	1.795PhePhe: 1.795 ± 0.009	2.262PheGly: 2.262 ± 0.011	1.061PheHis: 1.061 ± 0.005	2.157PheIle: 2.157 ± 0.009	1.917PheLys: 1.917 ± 0.007	4.101PheLeu: 4.101 ± 0.016	0.893PheMet: 0.893 ± 0.007	1.585PheAsn: 1.585 ± 0.007	1.83PhePro: 1.83 ± 0.008	1.666PheGln: 1.666 ± 0.006	1.903PheArg: 1.903 ± 0.009	3.45PheSer: 3.45 ± 0.012	2.445PheThr: 2.445 ± 0.01	2.382PheVal: 2.382 ± 0.009	0.494PheTrp: 0.494 ± 0.004	1.321PheTyr: 1.321 ± 0.007	0.0PheXaa: 0.0 ± 0.0
Gly	3.903GlyAla: 3.903 ± 0.015	1.213GlyCys: 1.213 ± 0.007	3.239GlyAsp: 3.239 ± 0.012	4.098GlyGlu: 4.098 ± 0.016	2.453GlyPhe: 2.453 ± 0.01	5.221GlyGly: 5.221 ± 0.024	1.629GlyHis: 1.629 ± 0.009	2.746GlyIle: 2.746 ± 0.01	3.642GlyLys: 3.642 ± 0.011	5.455GlyLeu: 5.455 ± 0.016	1.536GlyMet: 1.536 ± 0.007	2.51GlyAsn: 2.51 ± 0.011	3.097GlyPro: 3.097 ± 0.027	2.749GlyGln: 2.749 ± 0.011	3.527GlyArg: 3.527 ± 0.012	5.355GlySer: 5.355 ± 0.017	3.417GlyThr: 3.417 ± 0.014	4.036GlyVal: 4.036 ± 0.011	0.785GlyTrp: 0.785 ± 0.005	1.926GlyTyr: 1.926 ± 0.01	0.0GlyXaa: 0.0 ± 0.0
His	1.31HisAla: 1.31 ± 0.007	0.798HisCys: 0.798 ± 0.005	1.018HisAsp: 1.018 ± 0.007	1.221HisGlu: 1.221 ± 0.005	1.098HisPhe: 1.098 ± 0.006	1.549HisGly: 1.549 ± 0.007	1.037HisHis: 1.037 ± 0.008	1.421HisIle: 1.421 ± 0.006	1.288HisLys: 1.288 ± 0.007	2.712HisLeu: 2.712 ± 0.01	0.658HisMet: 0.658 ± 0.005	1.068HisAsn: 1.068 ± 0.007	1.538HisPro: 1.538 ± 0.008	1.217HisGln: 1.217 ± 0.007	1.602HisArg: 1.602 ± 0.007	2.356HisSer: 2.356 ± 0.011	1.698HisThr: 1.698 ± 0.009	1.474HisVal: 1.474 ± 0.007	0.343HisTrp: 0.343 ± 0.003	0.899HisTyr: 0.899 ± 0.005	0.0HisXaa: 0.0 ± 0.0
Ile	2.699IleAla: 2.699 ± 0.011	1.125IleCys: 1.125 ± 0.007	2.309IleAsp: 2.309 ± 0.008	2.553IleGlu: 2.553 ± 0.01	2.032IlePhe: 2.032 ± 0.011	2.425IleGly: 2.425 ± 0.009	1.366IleHis: 1.366 ± 0.007	2.726IleIle: 2.726 ± 0.013	2.708IleLys: 2.708 ± 0.01	4.648IleLeu: 4.648 ± 0.013	1.189IleMet: 1.189 ± 0.006	2.135IleAsn: 2.135 ± 0.008	2.503IlePro: 2.503 ± 0.01	2.31IleGln: 2.31 ± 0.01	2.601IleArg: 2.601 ± 0.009	3.91IleSer: 3.91 ± 0.012	2.963IleThr: 2.963 ± 0.012	2.783IleVal: 2.783 ± 0.01	0.506IleTrp: 0.506 ± 0.004	1.564IleTyr: 1.564 ± 0.008	0.0IleXaa: 0.0 ± 0.0
Lys	3.752LysAla: 3.752 ± 0.012	1.038LysCys: 1.038 ± 0.006	3.275LysAsp: 3.275 ± 0.011	4.651LysGlu: 4.651 ± 0.022	1.734LysPhe: 1.734 ± 0.008	3.191LysGly: 3.191 ± 0.015	1.413LysHis: 1.413 ± 0.006	2.644LysIle: 2.644 ± 0.01	4.333LysLys: 4.333 ± 0.021	5.158LysLeu: 5.158 ± 0.018	1.502LysMet: 1.502 ± 0.008	2.361LysAsn: 2.361 ± 0.009	2.749LysPro: 2.749 ± 0.011	2.565LysGln: 2.565 ± 0.012	3.264LysArg: 3.264 ± 0.012	3.66LysSer: 3.66 ± 0.011	3.241LysThr: 3.241 ± 0.011	3.624LysVal: 3.624 ± 0.012	0.598LysTrp: 0.598 ± 0.004	1.6LysTyr: 1.6 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	6.164LeuAla: 6.164 ± 0.018	2.322LeuCys: 2.322 ± 0.01	4.993LeuAsp: 4.993 ± 0.014	6.496LeuGlu: 6.496 ± 0.025	3.71LeuPhe: 3.71 ± 0.015	5.17LeuGly: 5.17 ± 0.014	2.756LeuHis: 2.756 ± 0.01	4.173LeuIle: 4.173 ± 0.014	5.669LeuLys: 5.669 ± 0.015	10.278LeuLeu: 10.278 ± 0.028	2.217LeuMet: 2.217 ± 0.008	3.763LeuAsn: 3.763 ± 0.011	5.096LeuPro: 5.096 ± 0.016	5.446LeuGln: 5.446 ± 0.019	5.67LeuArg: 5.67 ± 0.015	8.237LeuSer: 8.237 ± 0.02	5.445LeuThr: 5.445 ± 0.013	5.658LeuVal: 5.658 ± 0.015	1.103LeuTrp: 1.103 ± 0.007	2.804LeuTyr: 2.804 ± 0.009	0.0LeuXaa: 0.0 ± 0.0
Met	1.916MetAla: 1.916 ± 0.007	0.528MetCys: 0.528 ± 0.004	1.47MetAsp: 1.47 ± 0.007	2.018MetGlu: 2.018 ± 0.009	0.941MetPhe: 0.941 ± 0.006	1.442MetGly: 1.442 ± 0.007	0.507MetHis: 0.507 ± 0.004	0.962MetIle: 0.962 ± 0.005	1.51MetLys: 1.51 ± 0.007	2.206MetLeu: 2.206 ± 0.009	0.736MetMet: 0.736 ± 0.005	0.97MetAsn: 0.97 ± 0.006	1.07MetPro: 1.07 ± 0.008	1.0MetGln: 1.0 ± 0.005	1.217MetArg: 1.217 ± 0.006	1.837MetSer: 1.837 ± 0.008	1.328MetThr: 1.328 ± 0.006	1.67MetVal: 1.67 ± 0.008	0.28MetTrp: 0.28 ± 0.003	0.707MetTyr: 0.707 ± 0.004	0.0MetXaa: 0.0 ± 0.0
Asn	2.174AsnAla: 2.174 ± 0.008	0.928AsnCys: 0.928 ± 0.007	1.773AsnAsp: 1.773 ± 0.009	2.112AsnGlu: 2.112 ± 0.01	1.505AsnPhe: 1.505 ± 0.007	2.737AsnGly: 2.737 ± 0.013	1.035AsnHis: 1.035 ± 0.006	2.323AsnIle: 2.323 ± 0.009	2.279AsnLys: 2.279 ± 0.009	3.824AsnLeu: 3.824 ± 0.012	1.091AsnMet: 1.091 ± 0.006	1.861AsnAsn: 1.861 ± 0.008	2.263AsnPro: 2.263 ± 0.009	1.767AsnGln: 1.767 ± 0.007	2.025AsnArg: 2.025 ± 0.007	3.165AsnSer: 3.165 ± 0.01	2.369AsnThr: 2.369 ± 0.009	2.363AsnVal: 2.363 ± 0.009	0.471AsnTrp: 0.471 ± 0.004	1.23AsnTyr: 1.23 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	3.767ProAla: 3.767 ± 0.013	1.034ProCys: 1.034 ± 0.007	2.881ProAsp: 2.881 ± 0.011	3.638ProGlu: 3.638 ± 0.012	1.75ProPhe: 1.75 ± 0.009	3.873ProGly: 3.873 ± 0.025	1.456ProHis: 1.456 ± 0.007	1.902ProIle: 1.902 ± 0.008	2.43ProLys: 2.43 ± 0.014	4.704ProLeu: 4.704 ± 0.014	1.021ProMet: 1.021 ± 0.006	1.891ProAsn: 1.891 ± 0.008	4.926ProPro: 4.926 ± 0.03	2.503ProGln: 2.503 ± 0.012	2.547ProArg: 2.547 ± 0.01	5.02ProSer: 5.02 ± 0.018	2.954ProThr: 2.954 ± 0.014	3.673ProVal: 3.673 ± 0.012	0.553ProTrp: 0.553 ± 0.004	1.427ProTyr: 1.427 ± 0.007	0.0ProXaa: 0.0 ± 0.0
Gln	3.142GlnAla: 3.142 ± 0.012	1.03GlnCys: 1.03 ± 0.007	2.297GlnAsp: 2.297 ± 0.008	3.436GlnGlu: 3.436 ± 0.014	1.454GlnPhe: 1.454 ± 0.007	2.731GlnGly: 2.731 ± 0.011	1.325GlnHis: 1.325 ± 0.008	2.049GlnIle: 2.049 ± 0.009	2.515GlnLys: 2.515 ± 0.011	4.549GlnLeu: 4.549 ± 0.014	1.157GlnMet: 1.157 ± 0.006	1.746GlnAsn: 1.746 ± 0.007	2.403GlnPro: 2.403 ± 0.013	3.147GlnGln: 3.147 ± 0.025	3.031GlnArg: 3.031 ± 0.012	3.281GlnSer: 3.281 ± 0.01	2.54GlnThr: 2.54 ± 0.009	2.878GlnVal: 2.878 ± 0.01	0.579GlnTrp: 0.579 ± 0.004	1.348GlnTyr: 1.348 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.358ArgAla: 3.358 ± 0.011	1.255ArgCys: 1.255 ± 0.008	2.968ArgAsp: 2.968 ± 0.01	3.849ArgGlu: 3.849 ± 0.014	1.995ArgPhe: 1.995 ± 0.007	3.409ArgGly: 3.409 ± 0.014	1.528ArgHis: 1.528 ± 0.008	2.522ArgIle: 2.522 ± 0.009	3.469ArgLys: 3.469 ± 0.012	5.32ArgLeu: 5.32 ± 0.013	1.323ArgMet: 1.323 ± 0.006	2.164ArgAsn: 2.164 ± 0.008	2.768ArgPro: 2.768 ± 0.011	2.675ArgGln: 2.675 ± 0.01	4.036ArgArg: 4.036 ± 0.016	4.158ArgSer: 4.158 ± 0.014	2.89ArgThr: 2.89 ± 0.01	3.347ArgVal: 3.347 ± 0.011	0.703ArgTrp: 0.703 ± 0.005	1.629ArgTyr: 1.629 ± 0.008	0.0ArgXaa: 0.0 ± 0.0
Ser	5.211SerAla: 5.211 ± 0.013	1.925SerCys: 1.925 ± 0.011	4.213SerAsp: 4.213 ± 0.014	4.645SerGlu: 4.645 ± 0.014	3.13SerPhe: 3.13 ± 0.01	5.338SerGly: 5.338 ± 0.018	2.159SerHis: 2.159 ± 0.01	3.432SerIle: 3.432 ± 0.01	3.896SerLys: 3.896 ± 0.011	8.142SerLeu: 8.142 ± 0.019	1.778SerMet: 1.778 ± 0.007	2.933SerAsn: 2.933 ± 0.009	5.359SerPro: 5.359 ± 0.022	3.683SerGln: 3.683 ± 0.012	4.204SerArg: 4.204 ± 0.012	9.237SerSer: 9.237 ± 0.029	4.618SerThr: 4.618 ± 0.015	5.403SerVal: 5.403 ± 0.015	0.976SerTrp: 0.976 ± 0.006	2.198SerTyr: 2.198 ± 0.009	0.0SerXaa: 0.0 ± 0.0
Thr	3.959ThrAla: 3.959 ± 0.014	1.391ThrCys: 1.391 ± 0.011	2.98ThrAsp: 2.98 ± 0.013	3.738ThrGlu: 3.738 ± 0.011	2.213ThrPhe: 2.213 ± 0.009	3.763ThrGly: 3.763 ± 0.014	1.495ThrHis: 1.495 ± 0.008	2.606ThrIle: 2.606 ± 0.009	2.723ThrLys: 2.723 ± 0.011	5.517ThrLeu: 5.517 ± 0.013	1.27ThrMet: 1.27 ± 0.006	1.988ThrAsn: 1.988 ± 0.007	3.466ThrPro: 3.466 ± 0.015	2.371ThrGln: 2.371 ± 0.009	2.474ThrArg: 2.474 ± 0.009	4.605ThrSer: 4.605 ± 0.015	3.468ThrThr: 3.468 ± 0.033	4.381ThrVal: 4.381 ± 0.013	0.692ThrTrp: 0.692 ± 0.005	1.565ThrTyr: 1.565 ± 0.007	0.0ThrXaa: 0.0 ± 0.0
Val	4.219ValAla: 4.219 ± 0.013	1.824ValCys: 1.824 ± 0.01	3.297ValAsp: 3.297 ± 0.011	4.102ValGlu: 4.102 ± 0.012	2.844ValPhe: 2.844 ± 0.012	3.578ValGly: 3.578 ± 0.011	1.62ValHis: 1.62 ± 0.007	3.212ValIle: 3.212 ± 0.011	3.688ValLys: 3.688 ± 0.012	6.357ValLeu: 6.357 ± 0.017	1.605ValMet: 1.605 ± 0.007	2.583ValAsn: 2.583 ± 0.009	3.163ValPro: 3.163 ± 0.01	2.769ValGln: 2.769 ± 0.009	3.418ValArg: 3.418 ± 0.011	5.325ValSer: 5.325 ± 0.013	4.079ValThr: 4.079 ± 0.013	4.57ValVal: 4.57 ± 0.014	0.812ValTrp: 0.812 ± 0.005	1.941ValTyr: 1.941 ± 0.009	0.0ValXaa: 0.0 ± 0.0
Trp	0.69TrpAla: 0.69 ± 0.005	0.269TrpCys: 0.269 ± 0.003	0.667TrpAsp: 0.667 ± 0.005	0.774TrpGlu: 0.774 ± 0.005	0.476TrpPhe: 0.476 ± 0.004	0.635TrpGly: 0.635 ± 0.005	0.282TrpHis: 0.282 ± 0.003	0.599TrpIle: 0.599 ± 0.005	0.723TrpLys: 0.723 ± 0.004	1.208TrpLeu: 1.208 ± 0.006	0.367TrpMet: 0.367 ± 0.003	0.541TrpAsn: 0.541 ± 0.004	0.449TrpPro: 0.449 ± 0.004	0.516TrpGln: 0.516 ± 0.004	0.753TrpArg: 0.753 ± 0.005	0.958TrpSer: 0.958 ± 0.007	0.723TrpThr: 0.723 ± 0.005	0.716TrpVal: 0.716 ± 0.005	0.217TrpTrp: 0.217 ± 0.003	0.366TrpTyr: 0.366 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.503TyrAla: 1.503 ± 0.007	0.751TyrCys: 0.751 ± 0.005	1.499TyrAsp: 1.499 ± 0.007	1.726TyrGlu: 1.726 ± 0.008	1.321TyrPhe: 1.321 ± 0.007	1.757TyrGly: 1.757 ± 0.008	0.855TyrHis: 0.855 ± 0.006	1.652TyrIle: 1.652 ± 0.006	1.581TyrLys: 1.581 ± 0.009	2.842TyrLeu: 2.842 ± 0.01	0.728TyrMet: 0.728 ± 0.005	1.288TyrAsn: 1.288 ± 0.006	1.329TyrPro: 1.329 ± 0.007	1.312TyrGln: 1.312 ± 0.008	1.768TyrArg: 1.768 ± 0.008	2.377TyrSer: 2.377 ± 0.008	1.765TyrThr: 1.765 ± 0.007	1.704TyrVal: 1.704 ± 0.007	0.419TyrTrp: 0.419 ± 0.005	1.079TyrTyr: 1.079 ± 0.006	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 57547 proteins (37319313 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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