Amino acid dipepetide frequency for Pseudomonas phage Stalingrad

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.647AlaAla: 10.647 ± 1.219
0.64AlaCys: 0.64 ± 0.169
6.484AlaAsp: 6.484 ± 0.855
4.963AlaGlu: 4.963 ± 0.677
3.122AlaPhe: 3.122 ± 0.437
6.724AlaGly: 6.724 ± 0.968
1.681AlaHis: 1.681 ± 0.383
4.483AlaIle: 4.483 ± 0.611
6.484AlaLys: 6.484 ± 0.53
8.726AlaLeu: 8.726 ± 1.265
3.122AlaMet: 3.122 ± 0.606
3.442AlaAsn: 3.442 ± 0.494
3.522AlaPro: 3.522 ± 0.552
4.003AlaGln: 4.003 ± 0.54
6.164AlaArg: 6.164 ± 0.748
5.844AlaSer: 5.844 ± 0.604
5.764AlaThr: 5.764 ± 0.853
6.804AlaVal: 6.804 ± 0.676
1.441AlaTrp: 1.441 ± 0.5
2.882AlaTyr: 2.882 ± 0.655
0.0AlaXaa: 0.0 ± 0.0
Cys
1.041CysAla: 1.041 ± 0.275
0.0CysCys: 0.0 ± 0.0
0.801CysAsp: 0.801 ± 0.348
0.801CysGlu: 0.801 ± 0.275
0.64CysPhe: 0.64 ± 0.204
0.64CysGly: 0.64 ± 0.249
0.4CysHis: 0.4 ± 0.207
0.32CysIle: 0.32 ± 0.137
0.48CysLys: 0.48 ± 0.211
0.4CysLeu: 0.4 ± 0.153
0.24CysMet: 0.24 ± 0.207
0.32CysAsn: 0.32 ± 0.165
0.4CysPro: 0.4 ± 0.195
0.48CysGln: 0.48 ± 0.196
0.72CysArg: 0.72 ± 0.301
0.32CysSer: 0.32 ± 0.158
0.16CysThr: 0.16 ± 0.129
0.881CysVal: 0.881 ± 0.254
0.16CysTrp: 0.16 ± 0.109
0.08CysTyr: 0.08 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
6.324AspAla: 6.324 ± 0.973
0.801AspCys: 0.801 ± 0.254
4.803AspAsp: 4.803 ± 0.698
3.122AspGlu: 3.122 ± 0.517
3.202AspPhe: 3.202 ± 0.508
6.004AspGly: 6.004 ± 0.673
1.521AspHis: 1.521 ± 0.433
4.003AspIle: 4.003 ± 0.418
2.642AspLys: 2.642 ± 0.425
4.803AspLeu: 4.803 ± 0.68
1.841AspMet: 1.841 ± 0.303
2.402AspAsn: 2.402 ± 0.353
3.042AspPro: 3.042 ± 0.678
2.482AspGln: 2.482 ± 0.383
4.483AspArg: 4.483 ± 0.508
3.282AspSer: 3.282 ± 0.474
2.642AspThr: 2.642 ± 0.505
3.522AspVal: 3.522 ± 0.405
1.601AspTrp: 1.601 ± 0.377
1.921AspTyr: 1.921 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
7.925GluAla: 7.925 ± 1.004
0.64GluCys: 0.64 ± 0.211
4.323GluAsp: 4.323 ± 0.779
4.643GluGlu: 4.643 ± 0.67
2.402GluPhe: 2.402 ± 0.379
5.203GluGly: 5.203 ± 0.84
2.001GluHis: 2.001 ± 0.557
2.402GluIle: 2.402 ± 0.337
2.802GluLys: 2.802 ± 0.42
5.443GluLeu: 5.443 ± 0.733
2.001GluMet: 2.001 ± 0.419
2.402GluAsn: 2.402 ± 0.486
2.642GluPro: 2.642 ± 0.491
3.682GluGln: 3.682 ± 0.594
3.762GluArg: 3.762 ± 0.45
4.003GluSer: 4.003 ± 0.586
4.643GluThr: 4.643 ± 0.63
5.043GluVal: 5.043 ± 0.759
0.801GluTrp: 0.801 ± 0.256
2.001GluTyr: 2.001 ± 0.418
0.0GluXaa: 0.0 ± 0.0
Phe
2.482PheAla: 2.482 ± 0.479
0.56PheCys: 0.56 ± 0.205
2.962PheAsp: 2.962 ± 0.385
2.161PheGlu: 2.161 ± 0.495
1.121PhePhe: 1.121 ± 0.311
2.722PheGly: 2.722 ± 0.44
0.801PheHis: 0.801 ± 0.247
1.761PheIle: 1.761 ± 0.511
2.321PheLys: 2.321 ± 0.406
3.762PheLeu: 3.762 ± 0.483
0.72PheMet: 0.72 ± 0.164
2.001PheAsn: 2.001 ± 0.461
1.361PhePro: 1.361 ± 0.393
1.201PheGln: 1.201 ± 0.267
2.321PheArg: 2.321 ± 0.374
2.081PheSer: 2.081 ± 0.391
2.482PheThr: 2.482 ± 0.498
2.081PheVal: 2.081 ± 0.354
0.4PheTrp: 0.4 ± 0.227
0.72PheTyr: 0.72 ± 0.352
0.0PheXaa: 0.0 ± 0.0
Gly
7.125GlyAla: 7.125 ± 1.223
0.961GlyCys: 0.961 ± 0.252
5.043GlyAsp: 5.043 ± 0.71
6.084GlyGlu: 6.084 ± 0.76
3.362GlyPhe: 3.362 ± 0.478
6.244GlyGly: 6.244 ± 0.722
1.841GlyHis: 1.841 ± 0.389
4.643GlyIle: 4.643 ± 0.648
5.363GlyLys: 5.363 ± 0.976
6.404GlyLeu: 6.404 ± 0.608
1.521GlyMet: 1.521 ± 0.392
2.642GlyAsn: 2.642 ± 0.386
2.321GlyPro: 2.321 ± 0.503
3.522GlyGln: 3.522 ± 0.494
4.563GlyArg: 4.563 ± 0.59
4.803GlySer: 4.803 ± 0.51
4.963GlyThr: 4.963 ± 0.655
4.963GlyVal: 4.963 ± 0.61
1.841GlyTrp: 1.841 ± 0.331
2.241GlyTyr: 2.241 ± 0.44
0.0GlyXaa: 0.0 ± 0.0
His
1.281HisAla: 1.281 ± 0.279
0.4HisCys: 0.4 ± 0.192
1.681HisAsp: 1.681 ± 0.395
1.841HisGlu: 1.841 ± 0.338
1.121HisPhe: 1.121 ± 0.275
1.601HisGly: 1.601 ± 0.304
0.32HisHis: 0.32 ± 0.156
1.121HisIle: 1.121 ± 0.335
1.601HisLys: 1.601 ± 0.407
2.482HisLeu: 2.482 ± 0.491
0.801HisMet: 0.801 ± 0.199
0.64HisAsn: 0.64 ± 0.221
0.64HisPro: 0.64 ± 0.248
0.72HisGln: 0.72 ± 0.292
1.041HisArg: 1.041 ± 0.329
1.041HisSer: 1.041 ± 0.274
1.281HisThr: 1.281 ± 0.26
1.601HisVal: 1.601 ± 0.383
0.4HisTrp: 0.4 ± 0.197
0.56HisTyr: 0.56 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.163IleAla: 4.163 ± 0.465
0.64IleCys: 0.64 ± 0.232
3.202IleAsp: 3.202 ± 0.449
3.442IleGlu: 3.442 ± 0.447
0.56IlePhe: 0.56 ± 0.258
4.243IleGly: 4.243 ± 0.646
0.72IleHis: 0.72 ± 0.257
1.681IleIle: 1.681 ± 0.299
2.962IleLys: 2.962 ± 0.521
4.963IleLeu: 4.963 ± 0.678
1.281IleMet: 1.281 ± 0.305
1.361IleAsn: 1.361 ± 0.382
2.081IlePro: 2.081 ± 0.349
2.241IleGln: 2.241 ± 0.406
3.682IleArg: 3.682 ± 0.616
2.001IleSer: 2.001 ± 0.442
3.522IleThr: 3.522 ± 0.605
3.442IleVal: 3.442 ± 0.546
0.64IleTrp: 0.64 ± 0.179
1.441IleTyr: 1.441 ± 0.386
0.0IleXaa: 0.0 ± 0.0
Lys
7.125LysAla: 7.125 ± 0.982
0.48LysCys: 0.48 ± 0.184
3.842LysAsp: 3.842 ± 0.529
4.483LysGlu: 4.483 ± 0.867
2.562LysPhe: 2.562 ± 0.397
4.163LysGly: 4.163 ± 0.619
1.201LysHis: 1.201 ± 0.473
1.921LysIle: 1.921 ± 0.318
3.122LysLys: 3.122 ± 0.665
4.083LysLeu: 4.083 ± 0.663
1.601LysMet: 1.601 ± 0.308
1.841LysAsn: 1.841 ± 0.466
2.562LysPro: 2.562 ± 0.367
2.402LysGln: 2.402 ± 0.432
4.003LysArg: 4.003 ± 0.652
3.362LysSer: 3.362 ± 0.455
2.562LysThr: 2.562 ± 0.472
4.323LysVal: 4.323 ± 0.658
0.961LysTrp: 0.961 ± 0.238
1.521LysTyr: 1.521 ± 0.303
0.0LysXaa: 0.0 ± 0.0
Leu
7.125LeuAla: 7.125 ± 0.843
0.32LeuCys: 0.32 ± 0.204
4.243LeuAsp: 4.243 ± 0.554
6.564LeuGlu: 6.564 ± 0.781
2.722LeuPhe: 2.722 ± 0.446
5.363LeuGly: 5.363 ± 0.675
2.081LeuHis: 2.081 ± 0.322
4.403LeuIle: 4.403 ± 0.56
6.084LeuLys: 6.084 ± 0.647
5.123LeuLeu: 5.123 ± 0.621
2.802LeuMet: 2.802 ± 0.453
3.362LeuAsn: 3.362 ± 0.491
2.882LeuPro: 2.882 ± 0.435
3.762LeuGln: 3.762 ± 0.515
4.243LeuArg: 4.243 ± 0.428
5.043LeuSer: 5.043 ± 0.706
5.443LeuThr: 5.443 ± 0.627
4.963LeuVal: 4.963 ± 0.665
0.961LeuTrp: 0.961 ± 0.31
2.001LeuTyr: 2.001 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
3.522MetAla: 3.522 ± 0.531
0.16MetCys: 0.16 ± 0.122
1.841MetAsp: 1.841 ± 0.398
1.921MetGlu: 1.921 ± 0.327
0.64MetPhe: 0.64 ± 0.24
2.161MetGly: 2.161 ± 0.419
0.72MetHis: 0.72 ± 0.198
1.121MetIle: 1.121 ± 0.222
1.841MetLys: 1.841 ± 0.352
1.841MetLeu: 1.841 ± 0.407
0.24MetMet: 0.24 ± 0.175
0.48MetAsn: 0.48 ± 0.194
1.041MetPro: 1.041 ± 0.293
1.041MetGln: 1.041 ± 0.355
1.841MetArg: 1.841 ± 0.398
2.722MetSer: 2.722 ± 0.384
1.841MetThr: 1.841 ± 0.492
2.081MetVal: 2.081 ± 0.302
0.48MetTrp: 0.48 ± 0.2
0.801MetTyr: 0.801 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
4.323AsnAla: 4.323 ± 0.564
0.32AsnCys: 0.32 ± 0.201
1.921AsnAsp: 1.921 ± 0.327
2.081AsnGlu: 2.081 ± 0.396
1.281AsnPhe: 1.281 ± 0.263
4.643AsnGly: 4.643 ± 0.741
0.72AsnHis: 0.72 ± 0.298
2.161AsnIle: 2.161 ± 0.388
1.521AsnLys: 1.521 ± 0.365
2.802AsnLeu: 2.802 ± 0.439
0.64AsnMet: 0.64 ± 0.217
0.961AsnAsn: 0.961 ± 0.28
2.482AsnPro: 2.482 ± 0.405
1.281AsnGln: 1.281 ± 0.313
1.841AsnArg: 1.841 ± 0.305
1.281AsnSer: 1.281 ± 0.344
2.241AsnThr: 2.241 ± 0.43
3.522AsnVal: 3.522 ± 0.822
0.32AsnTrp: 0.32 ± 0.148
1.601AsnTyr: 1.601 ± 0.502
0.0AsnXaa: 0.0 ± 0.0
Pro
3.282ProAla: 3.282 ± 0.724
0.56ProCys: 0.56 ± 0.233
2.722ProAsp: 2.722 ± 0.475
4.163ProGlu: 4.163 ± 0.563
1.361ProPhe: 1.361 ± 0.301
2.321ProGly: 2.321 ± 0.344
1.201ProHis: 1.201 ± 0.351
1.761ProIle: 1.761 ± 0.294
2.402ProLys: 2.402 ± 0.509
2.241ProLeu: 2.241 ± 0.388
0.72ProMet: 0.72 ± 0.219
2.161ProAsn: 2.161 ± 0.269
0.881ProPro: 0.881 ± 0.345
1.761ProGln: 1.761 ± 0.336
2.081ProArg: 2.081 ± 0.39
1.921ProSer: 1.921 ± 0.355
2.161ProThr: 2.161 ± 0.448
3.282ProVal: 3.282 ± 0.538
0.801ProTrp: 0.801 ± 0.295
0.881ProTyr: 0.881 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
4.403GlnAla: 4.403 ± 0.761
0.32GlnCys: 0.32 ± 0.127
2.482GlnAsp: 2.482 ± 0.542
3.282GlnGlu: 3.282 ± 0.584
2.241GlnPhe: 2.241 ± 0.403
3.682GlnGly: 3.682 ± 0.553
0.801GlnHis: 0.801 ± 0.228
1.841GlnIle: 1.841 ± 0.401
2.402GlnLys: 2.402 ± 0.407
4.243GlnLeu: 4.243 ± 0.558
1.921GlnMet: 1.921 ± 0.422
1.441GlnAsn: 1.441 ± 0.313
1.361GlnPro: 1.361 ± 0.322
2.962GlnGln: 2.962 ± 0.879
2.081GlnArg: 2.081 ± 0.469
2.161GlnSer: 2.161 ± 0.374
1.441GlnThr: 1.441 ± 0.301
2.882GlnVal: 2.882 ± 0.433
0.56GlnTrp: 0.56 ± 0.217
1.201GlnTyr: 1.201 ± 0.356
0.0GlnXaa: 0.0 ± 0.0
Arg
4.803ArgAla: 4.803 ± 0.448
1.041ArgCys: 1.041 ± 0.321
4.403ArgAsp: 4.403 ± 0.594
3.042ArgGlu: 3.042 ± 0.445
2.081ArgPhe: 2.081 ± 0.39
4.723ArgGly: 4.723 ± 0.537
0.72ArgHis: 0.72 ± 0.238
2.722ArgIle: 2.722 ± 0.379
3.442ArgLys: 3.442 ± 0.518
5.043ArgLeu: 5.043 ± 0.608
1.921ArgMet: 1.921 ± 0.533
4.083ArgAsn: 4.083 ± 0.72
2.241ArgPro: 2.241 ± 0.506
2.722ArgGln: 2.722 ± 0.55
3.042ArgArg: 3.042 ± 0.547
3.762ArgSer: 3.762 ± 0.59
2.402ArgThr: 2.402 ± 0.392
4.163ArgVal: 4.163 ± 0.588
1.041ArgTrp: 1.041 ± 0.339
2.081ArgTyr: 2.081 ± 0.456
0.0ArgXaa: 0.0 ± 0.0
Ser
5.924SerAla: 5.924 ± 0.788
0.32SerCys: 0.32 ± 0.135
4.723SerAsp: 4.723 ± 0.53
2.642SerGlu: 2.642 ± 0.428
2.241SerPhe: 2.241 ± 0.385
5.363SerGly: 5.363 ± 0.764
1.441SerHis: 1.441 ± 0.36
3.362SerIle: 3.362 ± 0.501
2.802SerLys: 2.802 ± 0.548
3.602SerLeu: 3.602 ± 0.39
1.521SerMet: 1.521 ± 0.401
1.761SerAsn: 1.761 ± 0.341
2.882SerPro: 2.882 ± 0.43
1.921SerGln: 1.921 ± 0.372
2.882SerArg: 2.882 ± 0.386
3.282SerSer: 3.282 ± 0.599
2.402SerThr: 2.402 ± 0.67
3.682SerVal: 3.682 ± 0.498
0.881SerTrp: 0.881 ± 0.238
2.321SerTyr: 2.321 ± 0.588
0.0SerXaa: 0.0 ± 0.0
Thr
4.883ThrAla: 4.883 ± 0.671
0.4ThrCys: 0.4 ± 0.208
3.122ThrAsp: 3.122 ± 0.432
5.123ThrGlu: 5.123 ± 0.592
1.841ThrPhe: 1.841 ± 0.368
6.084ThrGly: 6.084 ± 0.756
0.961ThrHis: 0.961 ± 0.26
3.362ThrIle: 3.362 ± 0.454
2.802ThrLys: 2.802 ± 0.346
4.643ThrLeu: 4.643 ± 0.583
1.441ThrMet: 1.441 ± 0.272
1.601ThrAsn: 1.601 ± 0.285
2.642ThrPro: 2.642 ± 0.417
2.402ThrGln: 2.402 ± 0.458
2.642ThrArg: 2.642 ± 0.366
2.722ThrSer: 2.722 ± 0.52
3.202ThrThr: 3.202 ± 0.686
4.083ThrVal: 4.083 ± 0.772
1.041ThrTrp: 1.041 ± 0.452
1.681ThrTyr: 1.681 ± 0.472
0.0ThrXaa: 0.0 ± 0.0
Val
6.004ValAla: 6.004 ± 0.802
0.4ValCys: 0.4 ± 0.201
3.362ValAsp: 3.362 ± 0.464
5.604ValGlu: 5.604 ± 0.848
2.081ValPhe: 2.081 ± 0.382
4.723ValGly: 4.723 ± 0.587
2.081ValHis: 2.081 ± 0.345
3.202ValIle: 3.202 ± 0.618
4.963ValLys: 4.963 ± 0.646
5.283ValLeu: 5.283 ± 0.753
2.081ValMet: 2.081 ± 0.527
3.442ValAsn: 3.442 ± 0.632
2.321ValPro: 2.321 ± 0.454
2.402ValGln: 2.402 ± 0.324
5.123ValArg: 5.123 ± 0.586
3.682ValSer: 3.682 ± 0.441
4.883ValThr: 4.883 ± 0.584
5.043ValVal: 5.043 ± 0.808
0.881ValTrp: 0.881 ± 0.235
2.402ValTyr: 2.402 ± 0.397
0.0ValXaa: 0.0 ± 0.0
Trp
1.361TrpAla: 1.361 ± 0.328
0.08TrpCys: 0.08 ± 0.086
0.881TrpAsp: 0.881 ± 0.295
0.801TrpGlu: 0.801 ± 0.32
0.48TrpPhe: 0.48 ± 0.209
0.961TrpGly: 0.961 ± 0.215
0.56TrpHis: 0.56 ± 0.221
0.48TrpIle: 0.48 ± 0.132
0.801TrpLys: 0.801 ± 0.235
1.761TrpLeu: 1.761 ± 0.376
0.56TrpMet: 0.56 ± 0.163
0.56TrpAsn: 0.56 ± 0.196
0.32TrpPro: 0.32 ± 0.131
1.121TrpGln: 1.121 ± 0.31
0.881TrpArg: 0.881 ± 0.335
1.361TrpSer: 1.361 ± 0.382
1.281TrpThr: 1.281 ± 0.348
1.441TrpVal: 1.441 ± 0.474
0.16TrpTrp: 0.16 ± 0.106
0.32TrpTyr: 0.32 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.202TyrAla: 3.202 ± 0.462
0.24TyrCys: 0.24 ± 0.172
1.601TyrAsp: 1.601 ± 0.254
2.001TyrGlu: 2.001 ± 0.488
0.961TyrPhe: 0.961 ± 0.302
2.882TyrGly: 2.882 ± 0.479
0.4TyrHis: 0.4 ± 0.158
1.521TyrIle: 1.521 ± 0.296
1.281TyrLys: 1.281 ± 0.247
1.841TyrLeu: 1.841 ± 0.324
1.281TyrMet: 1.281 ± 0.339
1.041TyrAsn: 1.041 ± 0.286
1.041TyrPro: 1.041 ± 0.298
1.601TyrGln: 1.601 ± 0.28
2.081TyrArg: 2.081 ± 0.371
1.281TyrSer: 1.281 ± 0.375
1.361TyrThr: 1.361 ± 0.35
2.241TyrVal: 2.241 ± 0.531
0.801TyrTrp: 0.801 ± 0.296
0.48TyrTyr: 0.48 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12493 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski