Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4271 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q15X31|RRAB_PSEA6 Regulator of ribonuclease activity B OS=Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) OX=342610 GN=rraB PE=3 SV=1
MM1 pKa = 7.32 KK2 pKa = 10.35 LKK4 pKa = 10.88 YY5 pKa = 9.28 PALLALLSASAVQAGEE21 pKa = 4.08 LVISGVFDD29 pKa = 3.98 GPLPGGLPKK38 pKa = 10.7 GIEE41 pKa = 4.03 LFVTQDD47 pKa = 2.88 IADD50 pKa = 4.47 LSTCGVGSANNGGGTDD66 pKa = 3.44 NQEE69 pKa = 3.78 FTFPARR75 pKa = 11.84 AATAGSYY82 pKa = 9.91 IYY84 pKa = 10.25 LASEE88 pKa = 4.53 SPQFAAFFGFEE99 pKa = 3.84 PTYY102 pKa = 10.5 TNSAANINGDD112 pKa = 3.61 DD113 pKa = 4.74 AIEE116 pKa = 4.18 LFCDD120 pKa = 4.02 GAVVDD125 pKa = 4.5 VFGDD129 pKa = 3.53 INVDD133 pKa = 3.45 GNGEE137 pKa = 3.76 PWEE140 pKa = 4.14 YY141 pKa = 11.13 LDD143 pKa = 3.08 GWAYY147 pKa = 11.15 RR148 pKa = 11.84 NIDD151 pKa = 3.59 TGPDD155 pKa = 3.39 GNSFLLGNWTFSGPNAFDD173 pKa = 4.03 GEE175 pKa = 4.97 SNNATAAAPFPLKK188 pKa = 10.83 AFADD192 pKa = 4.78 EE193 pKa = 4.43 IDD195 pKa = 4.07 GGDD198 pKa = 3.34 TGGGSGGATDD208 pKa = 4.79 PSAPCFNCPALDD220 pKa = 4.82 PIADD224 pKa = 3.43 ASTFDD229 pKa = 3.66 SEE231 pKa = 4.61 SYY233 pKa = 9.54 YY234 pKa = 11.11 AAVSAEE240 pKa = 3.85 INANSPAATIKK251 pKa = 10.72 SAITSAITQGQKK263 pKa = 10.33 NLTYY267 pKa = 10.55 SEE269 pKa = 4.1 VWSALTHH276 pKa = 6.13 TDD278 pKa = 3.66 EE279 pKa = 6.2 DD280 pKa = 4.64 PNNPDD285 pKa = 5.86 NVILWYY291 pKa = 10.46 KK292 pKa = 8.02 GTSIAKK298 pKa = 9.52 SSNGSGSQSSDD309 pKa = 2.93 PDD311 pKa = 2.92 NWNRR315 pKa = 11.84 EE316 pKa = 4.06 HH317 pKa = 6.88 SWAKK321 pKa = 10.09 SHH323 pKa = 6.31 GFPNEE328 pKa = 3.86 SQEE331 pKa = 4.13 AYY333 pKa = 9.83 TDD335 pKa = 3.31 IHH337 pKa = 6.53 HH338 pKa = 7.15 LRR340 pKa = 11.84 PTDD343 pKa = 3.14 ISVNSSRR350 pKa = 11.84 GNLDD354 pKa = 3.51 FDD356 pKa = 4.23 NSDD359 pKa = 3.55 NALSEE364 pKa = 4.43 APSNRR369 pKa = 11.84 VDD371 pKa = 4.12 DD372 pKa = 5.33 DD373 pKa = 3.96 SFEE376 pKa = 4.31 PRR378 pKa = 11.84 DD379 pKa = 3.65 AVKK382 pKa = 10.97 GDD384 pKa = 3.46 VARR387 pKa = 11.84 MMFYY391 pKa = 9.97 MDD393 pKa = 2.94 TRR395 pKa = 11.84 YY396 pKa = 10.6 EE397 pKa = 4.2 GFGDD401 pKa = 3.82 STPDD405 pKa = 3.14 LVLVDD410 pKa = 4.65 RR411 pKa = 11.84 LTSTGAPEE419 pKa = 4.55 LGRR422 pKa = 11.84 LCRR425 pKa = 11.84 LVEE428 pKa = 3.79 WHH430 pKa = 6.58 NADD433 pKa = 3.77 PVDD436 pKa = 3.59 ATEE439 pKa = 3.82 QTRR442 pKa = 11.84 NDD444 pKa = 3.78 TIYY447 pKa = 10.54 EE448 pKa = 4.13 YY449 pKa = 10.72 QGNRR453 pKa = 11.84 NPFIDD458 pKa = 3.68 HH459 pKa = 7.07 PEE461 pKa = 3.97 WVDD464 pKa = 3.49 ILYY467 pKa = 7.51 QTPACGTDD475 pKa = 3.09 GGDD478 pKa = 3.77 TGGEE482 pKa = 4.07 DD483 pKa = 5.01 DD484 pKa = 5.38 GDD486 pKa = 3.93 TGGDD490 pKa = 3.32 TGGSASAGDD499 pKa = 3.73 IVISGVIDD507 pKa = 4.12 GPLSGGTPKK516 pKa = 10.62 AIEE519 pKa = 3.98 FFVRR523 pKa = 11.84 NDD525 pKa = 2.97 IADD528 pKa = 4.13 LSTCGFGSANNGGGSDD544 pKa = 3.51 GQEE547 pKa = 3.85 YY548 pKa = 8.62 TFSGSAAAGDD558 pKa = 4.43 FIYY561 pKa = 10.4 IASEE565 pKa = 3.98 GSNFDD570 pKa = 3.38 AYY572 pKa = 10.91 FGFSPTDD579 pKa = 3.16 TAGAAGINGDD589 pKa = 3.82 DD590 pKa = 4.97 AIEE593 pKa = 4.21 LFCDD597 pKa = 3.57 GDD599 pKa = 4.04 VVDD602 pKa = 5.34 VFGDD606 pKa = 3.37 INTDD610 pKa = 3.16 GTGEE614 pKa = 4.08 PWDD617 pKa = 4.75 HH618 pKa = 6.26 VDD620 pKa = 2.78 GWAYY624 pKa = 10.17 RR625 pKa = 11.84 VASTGPDD632 pKa = 2.96 GSTFTLSNWVFSGANALDD650 pKa = 4.07 GEE652 pKa = 4.64 ATNATAASQFPIGSYY667 pKa = 7.52 TSKK670 pKa = 10.76 EE671 pKa = 3.86 VLLISGVFDD680 pKa = 4.25 GPLSGGTPKK689 pKa = 10.6 AIEE692 pKa = 4.7 FYY694 pKa = 10.99 AVTDD698 pKa = 3.69 IQDD701 pKa = 3.42 LSVFGFGSANNGGGTDD717 pKa = 3.58 GQEE720 pKa = 4.09 FAFSGSVSAGEE731 pKa = 4.05 YY732 pKa = 10.15 FYY734 pKa = 11.56 VATEE738 pKa = 3.94 TSGFNAYY745 pKa = 10.3 FGFDD749 pKa = 3.25 PTFISGAAAINGDD762 pKa = 3.69 DD763 pKa = 4.52 AIEE766 pKa = 4.31 LFHH769 pKa = 7.16 EE770 pKa = 5.06 GEE772 pKa = 4.45 VVDD775 pKa = 4.5 VFGDD779 pKa = 3.58 INVDD783 pKa = 3.36 GTGQPWDD790 pKa = 4.16 YY791 pKa = 11.39 LDD793 pKa = 3.06 GWAYY797 pKa = 10.18 RR798 pKa = 11.84 VADD801 pKa = 4.23 SGPDD805 pKa = 3.04 GTTFVLANWTFSGTNVLDD823 pKa = 4.21 GSSNNATVSTPFPLGSFAGSGNNDD847 pKa = 3.14 GGDD850 pKa = 3.52 NGGGEE855 pKa = 4.35 TPLLGMCADD864 pKa = 4.23 PATLISAIQGSTDD877 pKa = 2.7 ISPAVGEE884 pKa = 3.94 THH886 pKa = 6.86 IIEE889 pKa = 5.31 GIVTASFPNLSGYY902 pKa = 9.38 FVQEE906 pKa = 3.87 EE907 pKa = 4.86 DD908 pKa = 3.53 SDD910 pKa = 3.95 QDD912 pKa = 3.4 ADD914 pKa = 3.76 SATSEE919 pKa = 4.42 GVFVYY924 pKa = 10.37 SPSLALPEE932 pKa = 4.24 AGKK935 pKa = 9.9 VVRR938 pKa = 11.84 VLGDD942 pKa = 3.1 VAEE945 pKa = 4.89 AFNKK949 pKa = 7.83 TQLVVSEE956 pKa = 4.75 INPDD960 pKa = 3.59 CGTGSVTATALTLPFDD976 pKa = 4.38 SVDD979 pKa = 3.36 SMEE982 pKa = 4.72 ALEE985 pKa = 4.5 GMLVASSGEE994 pKa = 3.96 LTVTDD999 pKa = 4.4 TYY1001 pKa = 11.8 SLGRR1005 pKa = 11.84 YY1006 pKa = 9.83 GEE1008 pKa = 4.31 VLLSNGRR1015 pKa = 11.84 LFIPTNLYY1023 pKa = 8.75 TAGSPEE1029 pKa = 4.73 AIALAAQNEE1038 pKa = 4.46 LNQITLDD1045 pKa = 4.43 DD1046 pKa = 4.51 GVNGQNPDD1054 pKa = 3.39 VVVYY1058 pKa = 8.7 PAGGLSASNPLRR1070 pKa = 11.84 GGDD1073 pKa = 3.44 TVTALTGVMDD1083 pKa = 4.83 FSFSLYY1089 pKa = 10.21 RR1090 pKa = 11.84 IIPVEE1095 pKa = 3.86 QPTIVASNPRR1105 pKa = 11.84 TDD1107 pKa = 4.89 APDD1110 pKa = 3.99 LEE1112 pKa = 4.89 LGNLKK1117 pKa = 10.16 VASLNVLNYY1126 pKa = 10.12 FNTLDD1131 pKa = 3.81 VSPNVCGPSNLEE1143 pKa = 3.7 CRR1145 pKa = 11.84 GADD1148 pKa = 3.55 SEE1150 pKa = 5.05 IEE1152 pKa = 4.28 LEE1154 pKa = 4.12 RR1155 pKa = 11.84 QRR1157 pKa = 11.84 AKK1159 pKa = 10.12 TLAALLAMDD1168 pKa = 4.99 ADD1170 pKa = 3.77 IVGLMEE1176 pKa = 4.16 VEE1178 pKa = 4.22 NNGFGANSAVGDD1190 pKa = 4.15 LVSGLNDD1197 pKa = 2.99 VLGANTYY1204 pKa = 8.85 ATVDD1208 pKa = 3.18 AGSAIGTDD1216 pKa = 4.2 AITVALIYY1224 pKa = 10.36 KK1225 pKa = 8.28 PAVVSVVGQPAILDD1239 pKa = 3.79 SSNSISDD1246 pKa = 3.56 DD1247 pKa = 4.06 DD1248 pKa = 4.92 GPLFLDD1254 pKa = 4.02 TKK1256 pKa = 10.83 NRR1258 pKa = 11.84 PALNQKK1264 pKa = 9.66 FALVANNEE1272 pKa = 4.11 EE1273 pKa = 4.23 LVVSVNHH1280 pKa = 6.38 FKK1282 pKa = 11.2 SKK1284 pKa = 10.55 GSSCGAGDD1292 pKa = 5.25 DD1293 pKa = 4.7 DD1294 pKa = 4.38 TTTGQGNCNLTRR1306 pKa = 11.84 TRR1308 pKa = 11.84 AAEE1311 pKa = 3.93 ALTTFLSQQFSDD1323 pKa = 3.9 TPTLIIGDD1331 pKa = 4.12 LNSYY1335 pKa = 10.95 AMEE1338 pKa = 4.17 EE1339 pKa = 4.48 PILKK1343 pKa = 9.73 ILQQGYY1349 pKa = 7.98 TDD1351 pKa = 4.4 LANKK1355 pKa = 10.27 FGGDD1359 pKa = 3.12 EE1360 pKa = 4.27 AYY1362 pKa = 10.29 SYY1364 pKa = 11.73 SFGGEE1369 pKa = 3.82 FGYY1372 pKa = 10.74 LDD1374 pKa = 3.95 HH1375 pKa = 7.3 ALASATLVDD1384 pKa = 4.24 QIVDD1388 pKa = 3.5 TTEE1391 pKa = 3.09 WHH1393 pKa = 6.54 INADD1397 pKa = 3.63 EE1398 pKa = 5.23 PIVFDD1403 pKa = 4.06 YY1404 pKa = 11.16 NVEE1407 pKa = 4.33 FKK1409 pKa = 10.85 SDD1411 pKa = 3.4 QQLIDD1416 pKa = 3.76 YY1417 pKa = 7.18 YY1418 pKa = 11.58 APDD1421 pKa = 4.21 AYY1423 pKa = 10.77 RR1424 pKa = 11.84 MSDD1427 pKa = 3.35 HH1428 pKa = 7.17 DD1429 pKa = 4.07 PVVISLLLEE1438 pKa = 4.22 SEE1440 pKa = 4.54 VQVVEE1445 pKa = 4.0 GDD1447 pKa = 3.35 YY1448 pKa = 11.53 DD1449 pKa = 4.23 GDD1451 pKa = 4.05 GDD1453 pKa = 4.35 VDD1455 pKa = 3.75 MMDD1458 pKa = 2.87 IRR1460 pKa = 11.84 ALTRR1464 pKa = 11.84 AIQLRR1469 pKa = 11.84 QTIDD1473 pKa = 2.93 NSFDD1477 pKa = 3.65 FNEE1480 pKa = 4.84 DD1481 pKa = 3.21 GQVSYY1486 pKa = 11.41 TDD1488 pKa = 3.24 VRR1490 pKa = 11.84 LLQRR1494 pKa = 11.84 MCTRR1498 pKa = 11.84 TRR1500 pKa = 11.84 CAII1503 pKa = 3.63
Molecular weight: 158.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.694
IPC_protein 3.745
Toseland 3.516
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.567
Grimsley 3.414
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.872
Patrickios 1.659
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|Q15MT0|Q15MT0_PSEA6 Flavodoxin/nitric oxide synthase OS=Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) OX=342610 GN=Patl_4309 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 NGRR28 pKa = 11.84 KK29 pKa = 9.48 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.34 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTAA44 pKa = 4.19
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4271
0
4271
1485885
33
4689
347.9
38.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.853 ± 0.035
0.981 ± 0.012
5.848 ± 0.032
5.786 ± 0.03
4.287 ± 0.025
6.82 ± 0.034
2.304 ± 0.023
6.189 ± 0.028
5.049 ± 0.037
10.114 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.021
4.499 ± 0.027
3.939 ± 0.02
4.775 ± 0.03
4.393 ± 0.029
6.912 ± 0.032
5.478 ± 0.05
6.913 ± 0.032
1.243 ± 0.015
3.108 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here