Proteome-pI 2.0 - Proteome Isoelectric Point Database

Hydrogenivirga sp. 128-5-R1-1

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Hydrogenivirga; unclassified Hydrogenivirga

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A8UVS0|A8UVS0_9AQUI Uncharacterized protein OS=Hydrogenivirga sp. 128-5-R1-1 OX=392423 GN=HG1285_00540 PE=4 SV=1

MM1 pKa = 8.89VMFDD5 pKa = 3.01WLKK8 pKa = 9.43RR9 pKa = 11.84WFWFDD14 pKa = 4.3PDD16 pKa = 5.24DD17 pKa = 6.71DD18 pKa = 5.79DD19 pKa = 6.8DD20 pKa = 5.55SLYY23 pKa = 11.07KK24 pKa = 10.57DD25 pKa = 4.37DD26 pKa = 4.7TDD28 pKa = 3.86SGNDD32 pKa = 3.31FVTDD36 pKa = 3.87PKK38 pKa = 11.45YY39 pKa = 11.1CFLEE43 pKa = 4.01GNIYY47 pKa = 10.52HH48 pKa = 7.27DD49 pKa = 4.0ICDD52 pKa = 3.89DD53 pKa = 3.73HH54 pKa = 8.16HH55 pKa = 6.99DD56 pKa = 4.33HH57 pKa = 7.08LWDD60 pKa = 5.38DD61 pKa = 4.2SDD63 pKa = 4.34SHH65 pKa = 6.88DD66 pKa = 3.74TTEE69 pKa = 4.8DD70 pKa = 2.53WSYY73 pKa = 11.8WDD75 pKa = 4.32DD76 pKa = 4.01TTDD79 pKa = 4.32DD80 pKa = 3.26WHH82 pKa = 6.3TTNDD86 pKa = 3.04WDD88 pKa = 3.6TWDD91 pKa = 5.03DD92 pKa = 5.12FSSSWDD98 pKa = 3.74DD99 pKa = 3.33DD100 pKa = 3.48WW101 pKa = 6.91

Molecular weight: 12.51 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A8UV95|A8UV95_9AQUI Transcriptional regulator (DegT/DnrJ/Eryc1 family) protein OS=Hydrogenivirga sp. 128-5-R1-1 OX=392423 GN=HG1285_16121 PE=3 SV=1

MM1 pKa = 7.76PEE3 pKa = 3.81ALKK6 pKa = 10.29PHH8 pKa = 6.65ISIKK12 pKa = 9.73KK13 pKa = 8.7KK14 pKa = 9.39KK15 pKa = 9.43RR16 pKa = 11.84KK17 pKa = 9.7SGFLARR23 pKa = 11.84MRR25 pKa = 11.84TRR27 pKa = 11.84SGRR30 pKa = 11.84KK31 pKa = 7.32IIKK34 pKa = 9.73RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84QKK39 pKa = 10.36GRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84LAPP46 pKa = 3.79

Molecular weight: 5.52 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3805	0	3805	957714	10	1535	251.7	28.59

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.984 ± 0.039	0.793 ± 0.017	4.975 ± 0.025	8.864 ± 0.057	4.902 ± 0.034	6.863 ± 0.037	1.554 ± 0.02	7.478 ± 0.056	8.408 ± 0.058	10.39 ± 0.052

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.052 ± 0.018	3.933 ± 0.034	3.848 ± 0.025	2.233 ± 0.022	5.317 ± 0.046	5.472 ± 0.034	4.353 ± 0.03	7.751 ± 0.038	0.923 ± 0.016	3.908 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski