Amino acid dipepetide frequency for Cucumis sativus (Cucumber)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.092AlaAla: 6.092 ± 0.041
1.187AlaCys: 1.187 ± 0.013
2.932AlaAsp: 2.932 ± 0.018
3.905AlaGlu: 3.905 ± 0.023
2.818AlaPhe: 2.818 ± 0.018
3.88AlaGly: 3.88 ± 0.025
1.272AlaHis: 1.272 ± 0.013
3.882AlaIle: 3.882 ± 0.025
3.704AlaLys: 3.704 ± 0.02
6.456AlaLeu: 6.456 ± 0.037
1.757AlaMet: 1.757 ± 0.016
2.55AlaAsn: 2.55 ± 0.019
2.673AlaPro: 2.673 ± 0.021
1.941AlaGln: 1.941 ± 0.016
3.203AlaArg: 3.203 ± 0.02
5.807AlaSer: 5.807 ± 0.028
3.549AlaThr: 3.549 ± 0.02
4.665AlaVal: 4.665 ± 0.026
0.722AlaTrp: 0.722 ± 0.009
1.727AlaTyr: 1.727 ± 0.016
0.0AlaXaa: 0.0 ± 0.0
Cys
0.945CysAla: 0.945 ± 0.011
0.53CysCys: 0.53 ± 0.01
0.844CysAsp: 0.844 ± 0.009
0.9CysGlu: 0.9 ± 0.011
0.924CysPhe: 0.924 ± 0.012
1.41CysGly: 1.41 ± 0.015
0.457CysHis: 0.457 ± 0.008
1.011CysIle: 1.011 ± 0.012
1.133CysLys: 1.133 ± 0.012
1.849CysLeu: 1.849 ± 0.017
0.422CysMet: 0.422 ± 0.007
0.868CysAsn: 0.868 ± 0.011
0.899CysPro: 0.899 ± 0.011
0.569CysGln: 0.569 ± 0.009
1.06CysArg: 1.06 ± 0.012
1.912CysSer: 1.912 ± 0.018
0.817CysThr: 0.817 ± 0.009
1.018CysVal: 1.018 ± 0.012
0.238CysTrp: 0.238 ± 0.005
0.546CysTyr: 0.546 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
3.254AspAla: 3.254 ± 0.018
0.961AspCys: 0.961 ± 0.011
3.57AspAsp: 3.57 ± 0.027
3.991AspGlu: 3.991 ± 0.031
2.393AspPhe: 2.393 ± 0.015
3.812AspGly: 3.812 ± 0.027
1.259AspHis: 1.259 ± 0.012
2.974AspIle: 2.974 ± 0.019
2.544AspLys: 2.544 ± 0.018
5.013AspLeu: 5.013 ± 0.028
1.246AspMet: 1.246 ± 0.012
2.073AspAsn: 2.073 ± 0.016
2.545AspPro: 2.545 ± 0.02
1.745AspGln: 1.745 ± 0.018
2.43AspArg: 2.43 ± 0.023
4.375AspSer: 4.375 ± 0.03
2.094AspThr: 2.094 ± 0.016
3.527AspVal: 3.527 ± 0.023
0.706AspTrp: 0.706 ± 0.011
1.519AspTyr: 1.519 ± 0.014
0.0AspXaa: 0.0 ± 0.0
Glu
4.549GluAla: 4.549 ± 0.028
0.88GluCys: 0.88 ± 0.011
3.889GluAsp: 3.889 ± 0.027
6.696GluGlu: 6.696 ± 0.084
2.442GluPhe: 2.442 ± 0.018
3.665GluGly: 3.665 ± 0.024
1.189GluHis: 1.189 ± 0.011
3.842GluIle: 3.842 ± 0.022
4.835GluLys: 4.835 ± 0.034
5.856GluLeu: 5.856 ± 0.032
1.844GluMet: 1.844 ± 0.018
3.198GluAsn: 3.198 ± 0.023
2.028GluPro: 2.028 ± 0.017
2.045GluGln: 2.045 ± 0.016
3.422GluArg: 3.422 ± 0.023
4.543GluSer: 4.543 ± 0.03
3.059GluThr: 3.059 ± 0.02
4.12GluVal: 4.12 ± 0.022
0.724GluTrp: 0.724 ± 0.009
1.573GluTyr: 1.573 ± 0.014
0.0GluXaa: 0.0 ± 0.0
Phe
2.487PheAla: 2.487 ± 0.019
0.955PheCys: 0.955 ± 0.011
2.443PheAsp: 2.443 ± 0.019
2.397PheGlu: 2.397 ± 0.017
2.314PhePhe: 2.314 ± 0.021
3.163PheGly: 3.163 ± 0.022
1.236PheHis: 1.236 ± 0.011
2.292PheIle: 2.292 ± 0.017
2.209PheLys: 2.209 ± 0.02
4.642PheLeu: 4.642 ± 0.024
0.969PheMet: 0.969 ± 0.01
1.937PheAsn: 1.937 ± 0.016
2.29PhePro: 2.29 ± 0.019
1.639PheGln: 1.639 ± 0.015
2.169PheArg: 2.169 ± 0.017
4.483PheSer: 4.483 ± 0.027
2.093PheThr: 2.093 ± 0.016
2.852PheVal: 2.852 ± 0.02
0.587PheTrp: 0.587 ± 0.008
1.317PheTyr: 1.317 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
3.702GlyAla: 3.702 ± 0.024
1.315GlyCys: 1.315 ± 0.014
3.369GlyAsp: 3.369 ± 0.024
3.685GlyGlu: 3.685 ± 0.025
3.268GlyPhe: 3.268 ± 0.024
5.602GlyGly: 5.602 ± 0.073
1.468GlyHis: 1.468 ± 0.016
3.686GlyIle: 3.686 ± 0.024
4.09GlyLys: 4.09 ± 0.021
5.819GlyLeu: 5.819 ± 0.028
1.493GlyMet: 1.493 ± 0.013
3.147GlyAsn: 3.147 ± 0.023
2.299GlyPro: 2.299 ± 0.019
1.944GlyGln: 1.944 ± 0.016
3.687GlyArg: 3.687 ± 0.026
5.944GlySer: 5.944 ± 0.033
3.063GlyThr: 3.063 ± 0.02
4.159GlyVal: 4.159 ± 0.024
0.9GlyTrp: 0.9 ± 0.011
2.018GlyTyr: 2.018 ± 0.017
0.0GlyXaa: 0.0 ± 0.0
His
1.283HisAla: 1.283 ± 0.013
0.529HisCys: 0.529 ± 0.009
1.119HisAsp: 1.119 ± 0.012
1.245HisGlu: 1.245 ± 0.013
1.17HisPhe: 1.17 ± 0.011
1.712HisGly: 1.712 ± 0.02
1.046HisHis: 1.046 ± 0.017
1.245HisIle: 1.245 ± 0.011
1.163HisLys: 1.163 ± 0.013
2.512HisLeu: 2.512 ± 0.018
0.538HisMet: 0.538 ± 0.008
1.046HisAsn: 1.046 ± 0.013
1.412HisPro: 1.412 ± 0.013
1.033HisGln: 1.033 ± 0.012
1.401HisArg: 1.401 ± 0.015
2.158HisSer: 2.158 ± 0.021
0.971HisThr: 0.971 ± 0.013
1.445HisVal: 1.445 ± 0.014
0.299HisTrp: 0.299 ± 0.007
0.72HisTyr: 0.72 ± 0.009
0.0HisXaa: 0.0 ± 0.0
Ile
3.577IleAla: 3.577 ± 0.025
1.121IleCys: 1.121 ± 0.012
2.953IleAsp: 2.953 ± 0.02
3.316IleGlu: 3.316 ± 0.02
2.493IlePhe: 2.493 ± 0.02
3.55IleGly: 3.55 ± 0.024
1.373IleHis: 1.373 ± 0.012
2.924IleIle: 2.924 ± 0.019
2.979IleLys: 2.979 ± 0.017
5.463IleLeu: 5.463 ± 0.026
1.173IleMet: 1.173 ± 0.012
2.296IleAsn: 2.296 ± 0.016
3.055IlePro: 3.055 ± 0.023
2.02IleGln: 2.02 ± 0.016
2.722IleArg: 2.722 ± 0.016
5.299IleSer: 5.299 ± 0.026
2.636IleThr: 2.636 ± 0.018
3.552IleVal: 3.552 ± 0.022
0.728IleTrp: 0.728 ± 0.009
1.532IleTyr: 1.532 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
3.933LysAla: 3.933 ± 0.024
0.977LysCys: 0.977 ± 0.012
3.19LysAsp: 3.19 ± 0.025
4.629LysGlu: 4.629 ± 0.034
2.34LysPhe: 2.34 ± 0.018
3.51LysGly: 3.51 ± 0.023
1.316LysHis: 1.316 ± 0.012
3.296LysIle: 3.296 ± 0.019
5.027LysLys: 5.027 ± 0.069
6.056LysLeu: 6.056 ± 0.033
1.561LysMet: 1.561 ± 0.014
2.816LysAsn: 2.816 ± 0.021
2.767LysPro: 2.767 ± 0.023
2.252LysGln: 2.252 ± 0.018
3.606LysArg: 3.606 ± 0.026
4.772LysSer: 4.772 ± 0.029
2.921LysThr: 2.921 ± 0.019
3.803LysVal: 3.803 ± 0.024
0.835LysTrp: 0.835 ± 0.011
1.639LysTyr: 1.639 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
6.286LeuAla: 6.286 ± 0.028
1.848LeuCys: 1.848 ± 0.017
4.963LeuAsp: 4.963 ± 0.032
6.215LeuGlu: 6.215 ± 0.039
4.136LeuPhe: 4.136 ± 0.025
5.683LeuGly: 5.683 ± 0.033
2.691LeuHis: 2.691 ± 0.017
4.854LeuIle: 4.854 ± 0.029
6.251LeuLys: 6.251 ± 0.031
10.144LeuLeu: 10.144 ± 0.048
2.143LeuMet: 2.143 ± 0.017
4.173LeuAsn: 4.173 ± 0.021
5.275LeuPro: 5.275 ± 0.025
4.257LeuGln: 4.257 ± 0.026
5.373LeuArg: 5.373 ± 0.029
8.796LeuSer: 8.796 ± 0.044
4.433LeuThr: 4.433 ± 0.025
6.092LeuVal: 6.092 ± 0.028
1.135LeuTrp: 1.135 ± 0.011
2.462LeuTyr: 2.462 ± 0.018
0.0LeuXaa: 0.0 ± 0.0
Met
2.222MetAla: 2.222 ± 0.014
0.304MetCys: 0.304 ± 0.006
1.422MetAsp: 1.422 ± 0.012
2.093MetGlu: 2.093 ± 0.019
0.831MetPhe: 0.831 ± 0.011
1.671MetGly: 1.671 ± 0.015
0.488MetHis: 0.488 ± 0.007
1.23MetIle: 1.23 ± 0.013
1.752MetLys: 1.752 ± 0.018
2.061MetLeu: 2.061 ± 0.015
0.667MetMet: 0.667 ± 0.01
1.06MetAsn: 1.06 ± 0.011
0.991MetPro: 0.991 ± 0.01
0.845MetGln: 0.845 ± 0.011
1.168MetArg: 1.168 ± 0.012
1.721MetSer: 1.721 ± 0.015
1.037MetThr: 1.037 ± 0.011
1.651MetVal: 1.651 ± 0.014
0.256MetTrp: 0.256 ± 0.005
0.579MetTyr: 0.579 ± 0.008
0.0MetXaa: 0.0 ± 0.0
Asn
2.634AsnAla: 2.634 ± 0.016
0.887AsnCys: 0.887 ± 0.012
2.19AsnAsp: 2.19 ± 0.019
2.647AsnGlu: 2.647 ± 0.019
2.183AsnPhe: 2.183 ± 0.017
3.406AsnGly: 3.406 ± 0.023
1.215AsnHis: 1.215 ± 0.012
2.541AsnIle: 2.541 ± 0.018
2.42AsnLys: 2.42 ± 0.017
4.741AsnLeu: 4.741 ± 0.033
1.081AsnMet: 1.081 ± 0.012
2.661AsnAsn: 2.661 ± 0.028
2.471AsnPro: 2.471 ± 0.018
1.783AsnGln: 1.783 ± 0.017
2.126AsnArg: 2.126 ± 0.016
4.315AsnSer: 4.315 ± 0.023
1.93AsnThr: 1.93 ± 0.015
2.837AsnVal: 2.837 ± 0.016
0.603AsnTrp: 0.603 ± 0.009
1.362AsnTyr: 1.362 ± 0.011
0.0AsnXaa: 0.0 ± 0.0
Pro
2.772ProAla: 2.772 ± 0.019
0.77ProCys: 0.77 ± 0.011
2.386ProAsp: 2.386 ± 0.017
2.886ProGlu: 2.886 ± 0.019
2.21ProPhe: 2.21 ± 0.017
2.488ProGly: 2.488 ± 0.02
1.168ProHis: 1.168 ± 0.012
2.574ProIle: 2.574 ± 0.021
2.744ProLys: 2.744 ± 0.019
4.475ProLeu: 4.475 ± 0.026
0.972ProMet: 0.972 ± 0.011
2.469ProAsn: 2.469 ± 0.017
4.383ProPro: 4.383 ± 0.077
1.775ProGln: 1.775 ± 0.018
2.3ProArg: 2.3 ± 0.017
5.51ProSer: 5.51 ± 0.034
2.806ProThr: 2.806 ± 0.023
2.927ProVal: 2.927 ± 0.022
0.595ProTrp: 0.595 ± 0.009
1.27ProTyr: 1.27 ± 0.015
0.0ProXaa: 0.0 ± 0.0
Gln
2.198GlnAla: 2.198 ± 0.019
0.561GlnCys: 0.561 ± 0.01
1.579GlnAsp: 1.579 ± 0.013
2.22GlnGlu: 2.22 ± 0.018
1.46GlnPhe: 1.46 ± 0.012
1.909GlnGly: 1.909 ± 0.014
0.887GlnHis: 0.887 ± 0.013
2.11GlnIle: 2.11 ± 0.016
2.315GlnLys: 2.315 ± 0.02
3.606GlnLeu: 3.606 ± 0.025
0.97GlnMet: 0.97 ± 0.011
1.828GlnAsn: 1.828 ± 0.017
1.734GlnPro: 1.734 ± 0.018
1.878GlnGln: 1.878 ± 0.032
2.085GlnArg: 2.085 ± 0.016
2.938GlnSer: 2.938 ± 0.022
1.75GlnThr: 1.75 ± 0.014
2.214GlnVal: 2.214 ± 0.015
0.469GlnTrp: 0.469 ± 0.007
0.92GlnTyr: 0.92 ± 0.01
0.0GlnXaa: 0.0 ± 0.0
Arg
3.118ArgAla: 3.118 ± 0.02
0.96ArgCys: 0.96 ± 0.011
2.608ArgAsp: 2.608 ± 0.021
3.308ArgGlu: 3.308 ± 0.023
2.327ArgPhe: 2.327 ± 0.015
3.145ArgGly: 3.145 ± 0.023
1.286ArgHis: 1.286 ± 0.014
2.921ArgIle: 2.921 ± 0.02
3.897ArgLys: 3.897 ± 0.024
5.008ArgLeu: 5.008 ± 0.026
1.345ArgMet: 1.345 ± 0.013
2.57ArgAsn: 2.57 ± 0.019
2.305ArgPro: 2.305 ± 0.02
1.817ArgGln: 1.817 ± 0.015
4.203ArgArg: 4.203 ± 0.03
4.476ArgSer: 4.476 ± 0.03
2.504ArgThr: 2.504 ± 0.017
3.195ArgVal: 3.195 ± 0.022
0.742ArgTrp: 0.742 ± 0.01
1.407ArgTyr: 1.407 ± 0.013
0.0ArgXaa: 0.0 ± 0.0
Ser
5.228SerAla: 5.228 ± 0.026
1.721SerCys: 1.721 ± 0.015
4.472SerAsp: 4.472 ± 0.023
4.795SerGlu: 4.795 ± 0.025
4.375SerPhe: 4.375 ± 0.026
5.829SerGly: 5.829 ± 0.035
2.172SerHis: 2.172 ± 0.019
4.922SerIle: 4.922 ± 0.027
5.115SerLys: 5.115 ± 0.028
8.971SerLeu: 8.971 ± 0.042
2.149SerMet: 2.149 ± 0.016
4.426SerAsn: 4.426 ± 0.027
4.924SerPro: 4.924 ± 0.042
3.064SerGln: 3.064 ± 0.022
4.564SerArg: 4.564 ± 0.029
12.475SerSer: 12.475 ± 0.115
4.983SerThr: 4.983 ± 0.026
5.242SerVal: 5.242 ± 0.028
1.173SerTrp: 1.173 ± 0.012
2.339SerTyr: 2.339 ± 0.019
0.0SerXaa: 0.0 ± 0.0
Thr
3.397ThrAla: 3.397 ± 0.022
0.892ThrCys: 0.892 ± 0.01
2.22ThrAsp: 2.22 ± 0.017
2.714ThrGlu: 2.714 ± 0.019
2.165ThrPhe: 2.165 ± 0.016
3.106ThrGly: 3.106 ± 0.018
1.085ThrHis: 1.085 ± 0.01
2.85ThrIle: 2.85 ± 0.018
2.728ThrLys: 2.728 ± 0.017
4.594ThrLeu: 4.594 ± 0.024
1.178ThrMet: 1.178 ± 0.012
2.262ThrAsn: 2.262 ± 0.017
2.677ThrPro: 2.677 ± 0.023
1.518ThrGln: 1.518 ± 0.015
2.286ThrArg: 2.286 ± 0.018
4.675ThrSer: 4.675 ± 0.027
3.127ThrThr: 3.127 ± 0.022
3.273ThrVal: 3.273 ± 0.022
0.611ThrTrp: 0.611 ± 0.008
1.323ThrTyr: 1.323 ± 0.015
0.0ThrXaa: 0.0 ± 0.0
Val
4.603ValAla: 4.603 ± 0.027
1.11ValCys: 1.11 ± 0.012
3.719ValAsp: 3.719 ± 0.021
4.448ValGlu: 4.448 ± 0.029
2.76ValPhe: 2.76 ± 0.019
4.215ValGly: 4.215 ± 0.023
1.467ValHis: 1.467 ± 0.013
3.408ValIle: 3.408 ± 0.019
3.854ValLys: 3.854 ± 0.02
6.074ValLeu: 6.074 ± 0.029
1.51ValMet: 1.51 ± 0.014
2.592ValAsn: 2.592 ± 0.017
3.027ValPro: 3.027 ± 0.021
2.19ValGln: 2.19 ± 0.017
3.031ValArg: 3.031 ± 0.022
5.431ValSer: 5.431 ± 0.025
3.029ValThr: 3.029 ± 0.021
4.95ValVal: 4.95 ± 0.031
0.758ValTrp: 0.758 ± 0.01
1.851ValTyr: 1.851 ± 0.014
0.0ValXaa: 0.0 ± 0.0
Trp
0.749TrpAla: 0.749 ± 0.009
0.227TrpCys: 0.227 ± 0.005
0.663TrpAsp: 0.663 ± 0.009
0.757TrpGlu: 0.757 ± 0.008
0.558TrpPhe: 0.558 ± 0.009
0.749TrpGly: 0.749 ± 0.01
0.277TrpHis: 0.277 ± 0.006
0.745TrpIle: 0.745 ± 0.009
0.956TrpLys: 0.956 ± 0.011
1.204TrpLeu: 1.204 ± 0.013
0.349TrpMet: 0.349 ± 0.006
0.733TrpAsn: 0.733 ± 0.012
0.509TrpPro: 0.509 ± 0.008
0.418TrpGln: 0.418 ± 0.007
0.839TrpArg: 0.839 ± 0.01
0.982TrpSer: 0.982 ± 0.011
0.63TrpThr: 0.63 ± 0.008
0.797TrpVal: 0.797 ± 0.009
0.238TrpTrp: 0.238 ± 0.006
0.331TrpTyr: 0.331 ± 0.006
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.697TyrAla: 1.697 ± 0.015
0.616TyrCys: 0.616 ± 0.009
1.5TyrAsp: 1.5 ± 0.014
1.552TyrGlu: 1.552 ± 0.013
1.311TyrPhe: 1.311 ± 0.013
2.152TyrGly: 2.152 ± 0.019
0.695TyrHis: 0.695 ± 0.011
1.459TyrIle: 1.459 ± 0.014
1.529TyrLys: 1.529 ± 0.019
2.666TyrLeu: 2.666 ± 0.02
0.68TyrMet: 0.68 ± 0.008
1.329TyrAsn: 1.329 ± 0.014
1.215TyrPro: 1.215 ± 0.014
0.894TyrGln: 0.894 ± 0.011
1.464TyrArg: 1.464 ± 0.014
2.327TyrSer: 2.327 ± 0.016
1.249TyrThr: 1.249 ± 0.015
1.71TyrVal: 1.71 ± 0.014
0.39TyrTrp: 0.39 ± 0.008
0.956TyrTyr: 0.956 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.012XaaXaa: 0.012 ± 0.006
Statistics based on 23744 proteins (8735437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski