Daejeonella lutea
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3561 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T5A1N8|A0A1T5A1N8_9SPHI TonB dependent receptor OS=Daejeonella lutea OX=572036 GN=SAMN05661099_0211 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.47 EE3 pKa = 3.46 NDD5 pKa = 3.95 FYY7 pKa = 11.66 LSTEE11 pKa = 3.57 IKK13 pKa = 10.34 YY14 pKa = 10.71 NCLDD18 pKa = 3.68 VVKK21 pKa = 9.83 MWGTDD26 pKa = 3.77 FEE28 pKa = 5.18 LKK30 pKa = 10.52 VYY32 pKa = 10.86 SLTDD36 pKa = 2.9 TDD38 pKa = 4.64 YY39 pKa = 11.43 EE40 pKa = 4.23 VAEE43 pKa = 4.33 NCIEE47 pKa = 4.15 LYY49 pKa = 10.74 GDD51 pKa = 3.95 SRR53 pKa = 11.84 PDD55 pKa = 3.35 LYY57 pKa = 11.36 AFEE60 pKa = 4.85 VSLDD64 pKa = 3.78 DD65 pKa = 4.09 EE66 pKa = 4.75 LSYY69 pKa = 11.28 DD70 pKa = 3.84 YY71 pKa = 11.03 MNDD74 pKa = 3.51 AITWYY79 pKa = 9.83 ASHH82 pKa = 7.02 IGKK85 pKa = 9.24 PDD87 pKa = 3.43 MEE89 pKa = 4.75 ILRR92 pKa = 11.84 TDD94 pKa = 3.12 PRR96 pKa = 11.84 EE97 pKa = 4.13 DD98 pKa = 3.49 FLEE101 pKa = 4.93 GSLPKK106 pKa = 10.66 VGII109 pKa = 4.09
Molecular weight: 12.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.719
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.63
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 1.036
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.936
Protein with the highest isoelectric point:
>tr|A0A1T5ARF0|A0A1T5ARF0_9SPHI 2'-5' RNA ligase OS=Daejeonella lutea OX=572036 GN=SAMN05661099_0951 PE=4 SV=1
MM1 pKa = 7.63 RR2 pKa = 11.84 FSFIVLRR9 pKa = 11.84 KK10 pKa = 8.63 NRR12 pKa = 11.84 KK13 pKa = 7.79 FLRR16 pKa = 11.84 LLFASASLFVRR27 pKa = 11.84 FRR29 pKa = 11.84 FAFCSLCFRR38 pKa = 11.84 VCSEE42 pKa = 3.83 TASYY46 pKa = 11.01 LLRR49 pKa = 11.84 DD50 pKa = 3.78 RR51 pKa = 11.84 FGKK54 pKa = 10.31 ASCIFRR60 pKa = 11.84 LFFGRR65 pKa = 11.84 KK66 pKa = 6.61 FNRR69 pKa = 11.84 VSTDD73 pKa = 3.43 FVYY76 pKa = 10.96 SSVPVLRR83 pKa = 11.84 GNAGYY88 pKa = 7.92 RR89 pKa = 11.84 TASEE93 pKa = 4.48 GDD95 pKa = 3.5 LKK97 pKa = 11.02 NCRR100 pKa = 11.84 IRR102 pKa = 11.84 LGVGNNLISITYY114 pKa = 9.9 DD115 pKa = 2.96 KK116 pKa = 10.79 RR117 pKa = 11.84 SKK119 pKa = 10.86 NDD121 pKa = 3.18 QILVSRR127 pKa = 11.84 KK128 pKa = 7.32 TLSQSYY134 pKa = 10.41 LSDD137 pKa = 3.8 NFRR140 pKa = 11.84 QDD142 pKa = 2.61 IKK144 pKa = 10.95 CQEE147 pKa = 4.07 KK148 pKa = 10.85 LPTGVLLKK156 pKa = 10.82 AILYY160 pKa = 8.71 LII162 pKa = 4.6
Molecular weight: 18.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.736
IPC_protein 10.452
Toseland 10.643
ProMoST 10.321
Dawson 10.76
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 11.038
Grimsley 10.804
Solomon 10.847
Lehninger 10.818
Nozaki 10.657
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.038
Sillero 10.687
Patrickios 10.745
IPC_peptide 10.847
IPC2_peptide 9.633
IPC2.peptide.svr19 8.482
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3561
0
3561
1196146
38
3472
335.9
37.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.285 ± 0.038
0.762 ± 0.015
5.416 ± 0.025
5.85 ± 0.044
4.957 ± 0.03
7.038 ± 0.041
1.779 ± 0.019
7.438 ± 0.035
6.909 ± 0.036
9.426 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.362 ± 0.02
5.43 ± 0.039
3.896 ± 0.029
3.376 ± 0.023
4.253 ± 0.026
6.888 ± 0.032
5.607 ± 0.05
6.437 ± 0.03
1.097 ± 0.015
3.795 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here