Brachybacterium nesterenkovii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X6X8E5|A0A1X6X8E5_9MICO Cytidine deaminase OS=Brachybacterium nesterenkovii OX=47847 GN=FM110_12780 PE=4 SV=1
MM1 pKa = 7.7PGAAYY6 pKa = 7.85PATATGAIPAQPGAMPVHH24 pKa = 6.81PGGFGVPGAMPTMTGAIPAQQTGQLPTATGAIPVQPTAQLPTATGAIPAQSAGPAPSALAPHH86 pKa = 6.59EE87 pKa = 5.14LVAQPWPAVPGTTHH101 pKa = 7.02GSGAPRR107 pKa = 11.84SRR109 pKa = 11.84AGLVAAIAGLCALLLVLLAGLTAAGIYY136 pKa = 9.33VAASLQSMEE145 pKa = 4.2GTIEE149 pKa = 3.87QAPAPGTPGGDD160 pKa = 3.31EE161 pKa = 4.16PQTVPGTVLDD171 pKa = 3.94SSGAPVDD178 pKa = 4.2GLGTPDD184 pKa = 3.65APAIMGEE191 pKa = 4.04NSLRR195 pKa = 11.84WPTEE199 pKa = 3.78DD200 pKa = 4.79GGTLTLEE207 pKa = 4.39IEE209 pKa = 4.56SVTWNADD216 pKa = 3.08EE217 pKa = 5.64DD218 pKa = 4.2IKK220 pKa = 11.44ASDD223 pKa = 3.91PSAPAPEE230 pKa = 5.83AGMHH234 pKa = 5.84YY235 pKa = 11.09ALITIRR241 pKa = 11.84GTYY244 pKa = 9.52EE245 pKa = 3.71GEE247 pKa = 4.07KK248 pKa = 10.43YY249 pKa = 10.57AVLGDD254 pKa = 4.97DD255 pKa = 3.56IDD257 pKa = 4.48LAIEE261 pKa = 3.98TDD263 pKa = 3.01AGVYY267 pKa = 10.39YY268 pKa = 10.66DD269 pKa = 5.07DD270 pKa = 4.23GQIRR274 pKa = 11.84APEE277 pKa = 4.1PLWRR281 pKa = 11.84MGGITDD287 pKa = 4.27GEE289 pKa = 4.57SASGQMVIAVPEE301 pKa = 4.22AEE303 pKa = 4.27VGSALVSVAPWGGDD317 pKa = 3.24YY318 pKa = 10.95LYY320 pKa = 10.94VAEE323 pKa = 4.55SS324 pKa = 3.19

Molecular weight: 32.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X6X943|A0A1X6X943_9MICO Uncharacterized protein OS=Brachybacterium nesterenkovii OX=47847 GN=FM110_13165 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TTLTTTHH9 pKa = 7.02RR10 pKa = 11.84AITMRR15 pKa = 11.84TTLTVTLGTVAIGAALAIALGTVTVGTLRR44 pKa = 11.84AVAIGALLAIALRR57 pKa = 11.84AVSIGPALSVTPRR70 pKa = 11.84SITVGPTLPTTLGAVTVGPTLPIALRR96 pKa = 11.84PIAIGATLAVTPRR109 pKa = 11.84PITVRR114 pKa = 11.84TTLALALGTVTVGTLRR130 pKa = 11.84AVAIGALLAIALRR143 pKa = 11.84PIPIRR148 pKa = 11.84TTLALALGAITVGPTLPVTLRR169 pKa = 11.84PITIRR174 pKa = 11.84TTLPIPLRR182 pKa = 11.84ATAVGPTLPVTLRR195 pKa = 11.84TITVRR200 pKa = 11.84TTLPIPLGTVTVGTLRR216 pKa = 11.84AVAIGATLAVTPRR229 pKa = 11.84PITVRR234 pKa = 11.84TALALALRR242 pKa = 11.84TITVGPTLPVTLRR255 pKa = 11.84PITVGPTLTITLGAITVRR273 pKa = 11.84TTLPIPLGAITVRR286 pKa = 11.84TTLPITLRR294 pKa = 11.84TIPVRR299 pKa = 11.84PTPTIVSRR307 pKa = 11.84RR308 pKa = 11.84AAAGAHH314 pKa = 6.37AARR317 pKa = 11.84ALVARR322 pKa = 11.84SVVVSVFHH330 pKa = 6.56DD331 pKa = 3.57QPLFASADD339 pKa = 3.47VRR341 pKa = 11.84AGAVVPPRR349 pKa = 3.73

Molecular weight: 35.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2675

0

2675

870157

37

2070

325.3

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.304 ± 0.073

0.651 ± 0.013

6.405 ± 0.043

6.029 ± 0.044

2.563 ± 0.031

9.242 ± 0.046

2.113 ± 0.02

4.168 ± 0.031

1.64 ± 0.037

10.16 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.005 ± 0.022

1.445 ± 0.02

5.842 ± 0.036

2.671 ± 0.025

8.118 ± 0.058

5.402 ± 0.035

5.896 ± 0.036

8.343 ± 0.043

1.34 ± 0.018

1.663 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski