Sewage-associated circular DNA virus-21

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI44|A0A0B4UI44_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-21 OX=1592088 PE=3 SV=1
MM1 pKa = 7.83SSRR4 pKa = 11.84AWCFTLNNYY13 pKa = 9.94SEE15 pKa = 5.67DD16 pKa = 3.77DD17 pKa = 3.69LFEE20 pKa = 5.67ISGWDD25 pKa = 3.33CKK27 pKa = 10.6YY28 pKa = 10.38VVYY31 pKa = 10.14GVEE34 pKa = 4.13VAPEE38 pKa = 3.73TGTRR42 pKa = 11.84HH43 pKa = 4.76LQGYY47 pKa = 10.0CMFLGVQRR55 pKa = 11.84LSAVKK60 pKa = 10.24KK61 pKa = 10.43LLEE64 pKa = 4.09TAHH67 pKa = 6.32WEE69 pKa = 4.0IARR72 pKa = 11.84GSPLQNRR79 pKa = 11.84TYY81 pKa = 9.29CTKK84 pKa = 10.32EE85 pKa = 3.43GDD87 pKa = 3.78FVEE90 pKa = 5.21VGTLPLPRR98 pKa = 11.84GEE100 pKa = 4.29SEE102 pKa = 3.9KK103 pKa = 10.75EE104 pKa = 3.26RR105 pKa = 11.84WAEE108 pKa = 3.55AFEE111 pKa = 4.2AAKK114 pKa = 10.27HH115 pKa = 5.48GRR117 pKa = 11.84LDD119 pKa = 5.85DD120 pKa = 3.77IPEE123 pKa = 4.91DD124 pKa = 3.22IRR126 pKa = 11.84VRR128 pKa = 11.84YY129 pKa = 8.19YY130 pKa = 10.35RR131 pKa = 11.84TMKK134 pKa = 10.17EE135 pKa = 3.44IKK137 pKa = 8.78KK138 pKa = 9.97DD139 pKa = 3.59HH140 pKa = 5.62MVKK143 pKa = 10.6PEE145 pKa = 4.01DD146 pKa = 3.73QQDD149 pKa = 3.66VTGEE153 pKa = 3.9WFYY156 pKa = 11.85GEE158 pKa = 4.23PGVGKK163 pKa = 9.87SHH165 pKa = 7.95LARR168 pKa = 11.84EE169 pKa = 4.36QYY171 pKa = 10.04PGAYY175 pKa = 10.26LKK177 pKa = 9.28MQNKK181 pKa = 7.88WWDD184 pKa = 3.65GYY186 pKa = 10.21QDD188 pKa = 3.6EE189 pKa = 5.5KK190 pKa = 11.67YY191 pKa = 11.06VILDD195 pKa = 3.9DD196 pKa = 4.75FDD198 pKa = 5.07CKK200 pKa = 10.88EE201 pKa = 4.46LGHH204 pKa = 6.56HH205 pKa = 6.25LKK207 pKa = 9.94IWGDD211 pKa = 3.33KK212 pKa = 10.54YY213 pKa = 11.64SFLAEE218 pKa = 4.23TKK220 pKa = 10.47GGAICIRR227 pKa = 11.84PEE229 pKa = 3.84KK230 pKa = 10.78LIVTSNYY237 pKa = 9.74KK238 pKa = 10.2PEE240 pKa = 3.98QLFSDD245 pKa = 3.84EE246 pKa = 3.99MLIAVKK252 pKa = 10.35RR253 pKa = 11.84RR254 pKa = 11.84FKK256 pKa = 9.56VTHH259 pKa = 5.07VLSWRR264 pKa = 11.84AVTNPPVV271 pKa = 3.05

Molecular weight: 31.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI44|A0A0B4UI44_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-21 OX=1592088 PE=3 SV=1
MM1 pKa = 7.36VKK3 pKa = 10.03RR4 pKa = 11.84GYY6 pKa = 10.45GRR8 pKa = 11.84WRR10 pKa = 11.84AAAIGAGAAVVARR23 pKa = 11.84GARR26 pKa = 11.84YY27 pKa = 8.51AVKK30 pKa = 10.24RR31 pKa = 11.84VRR33 pKa = 11.84RR34 pKa = 11.84YY35 pKa = 7.56MKK37 pKa = 10.16SRR39 pKa = 11.84PTVGRR44 pKa = 11.84KK45 pKa = 8.99RR46 pKa = 11.84KK47 pKa = 9.25MNMSKK52 pKa = 10.3PRR54 pKa = 11.84IAKK57 pKa = 9.61KK58 pKa = 10.39SRR60 pKa = 11.84GFGRR64 pKa = 11.84KK65 pKa = 8.64RR66 pKa = 11.84FGGLPNYY73 pKa = 10.11LNQGPGNEE81 pKa = 3.94LYY83 pKa = 9.69QWKK86 pKa = 8.17RR87 pKa = 11.84TARR90 pKa = 11.84IQRR93 pKa = 11.84PTLKK97 pKa = 10.39KK98 pKa = 9.9FLYY101 pKa = 6.78PTTISRR107 pKa = 11.84QDD109 pKa = 3.12RR110 pKa = 11.84FQGLTNFDD118 pKa = 3.92TNGGYY123 pKa = 10.71ARR125 pKa = 11.84LSYY128 pKa = 9.14HH129 pKa = 5.46TSIGEE134 pKa = 3.85NDD136 pKa = 3.96PRR138 pKa = 11.84WLPMAIFDD146 pKa = 4.13LTSFNNDD153 pKa = 2.74GAVPPYY159 pKa = 11.11SNVGYY164 pKa = 10.85KK165 pKa = 10.48LGWQNSGFGADD176 pKa = 3.69YY177 pKa = 10.7ARR179 pKa = 11.84SSIRR183 pKa = 11.84GQVPDD188 pKa = 4.65GDD190 pKa = 3.91ATTNTWTITSGTDD203 pKa = 2.76NGIYY207 pKa = 10.67NNISKK212 pKa = 10.56VYY214 pKa = 10.01LKK216 pKa = 10.38WIDD219 pKa = 3.09IRR221 pKa = 11.84LNLYY225 pKa = 8.66GARR228 pKa = 11.84KK229 pKa = 8.55RR230 pKa = 11.84TTKK233 pKa = 10.58FEE235 pKa = 4.01AQLVQFSNDD244 pKa = 2.93FGNLHH249 pKa = 6.54MGPLDD254 pKa = 3.85NPAGKK259 pKa = 10.22NNISYY264 pKa = 8.92MEE266 pKa = 4.38RR267 pKa = 11.84PLLFNNLQISTHH279 pKa = 6.16KK280 pKa = 8.94PEE282 pKa = 4.36KK283 pKa = 9.95MRR285 pKa = 11.84VLKK288 pKa = 10.05KK289 pKa = 9.79WKK291 pKa = 7.97WTVGPTTSIDD301 pKa = 3.71LNTTTGKK308 pKa = 9.56IQEE311 pKa = 4.16AKK313 pKa = 10.39IFLKK317 pKa = 10.13MNKK320 pKa = 9.64AINLDD325 pKa = 3.64YY326 pKa = 11.24SVGNNAVHH334 pKa = 6.61VDD336 pKa = 3.41VGDD339 pKa = 3.92GADD342 pKa = 3.63YY343 pKa = 11.29DD344 pKa = 3.86VDD346 pKa = 3.49TTTRR350 pKa = 11.84HH351 pKa = 6.63RR352 pKa = 11.84DD353 pKa = 3.44PKK355 pKa = 10.13PPSRR359 pKa = 11.84VYY361 pKa = 11.1LLLKK365 pKa = 10.12AFCPALTSLPSAALWDD381 pKa = 3.61KK382 pKa = 10.19TLAEE386 pKa = 4.56GSISNPFGGTALNNADD402 pKa = 3.92EE403 pKa = 4.56PSFDD407 pKa = 3.31FMIRR411 pKa = 11.84RR412 pKa = 11.84KK413 pKa = 10.04YY414 pKa = 10.69YY415 pKa = 9.92CDD417 pKa = 3.36KK418 pKa = 11.37

Molecular weight: 47.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

689

271

418

344.5

39.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.676 ± 0.674

1.161 ± 0.622

5.951 ± 0.19

4.79 ± 2.619

3.919 ± 0.135

8.273 ± 0.527

1.887 ± 0.629

4.499 ± 0.26

7.837 ± 0.166

7.547 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.064

5.37 ± 1.864

5.08 ± 0.385

2.903 ± 0.247

7.257 ± 0.799

5.225 ± 0.471

6.241 ± 0.853

5.515 ± 0.883

2.612 ± 0.418

4.935 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski