Xinzhou dimarhabdovirus virus 1
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KND6|A0A1L3KND6_9VIRU Putative glycoprotein OS=Xinzhou dimarhabdovirus virus 1 OX=1923768 PE=4 SV=1
MM1 pKa = 7.29 LTLISTVLFLLSSKK15 pKa = 10.26 FVLGGLIIPLCRR27 pKa = 11.84 SGPLWHH33 pKa = 7.31 KK34 pKa = 10.72 INPTDD39 pKa = 3.96 MICPRR44 pKa = 11.84 YY45 pKa = 9.74 EE46 pKa = 3.97 SGPPEE51 pKa = 4.71 GEE53 pKa = 3.9 MNMTDD58 pKa = 2.98 IQTFDD63 pKa = 3.18 IPAISGKK70 pKa = 8.38 QLGYY74 pKa = 9.88 LCHH77 pKa = 5.88 GVIYY81 pKa = 10.14 RR82 pKa = 11.84 VICIKK87 pKa = 10.78 GVFGGEE93 pKa = 4.33 TINKK97 pKa = 9.2 VILPTKK103 pKa = 9.21 ITPNEE108 pKa = 3.92 CEE110 pKa = 4.3 VAVKK114 pKa = 10.26 EE115 pKa = 4.13 YY116 pKa = 9.89 LTTAEE121 pKa = 4.53 DD122 pKa = 3.43 HH123 pKa = 6.71 RR124 pKa = 11.84 SGYY127 pKa = 10.26 FPGKK131 pKa = 8.0 YY132 pKa = 8.9 CVYY135 pKa = 9.97 EE136 pKa = 3.82 ILSTHH141 pKa = 6.0 TEE143 pKa = 3.81 FSDD146 pKa = 3.33 KK147 pKa = 11.01 KK148 pKa = 10.11 FILVSDD154 pKa = 3.73 HH155 pKa = 6.05 TVLYY159 pKa = 10.53 DD160 pKa = 3.79 PYY162 pKa = 10.59 ADD164 pKa = 3.39 QWIDD168 pKa = 3.34 TLFLGGRR175 pKa = 11.84 CDD177 pKa = 3.5 TRR179 pKa = 11.84 SCRR182 pKa = 11.84 TIKK185 pKa = 10.86 DD186 pKa = 3.55 SVVWISNVVKK196 pKa = 9.29 PACPKK201 pKa = 9.99 AVSLLVHH208 pKa = 6.02 VAYY211 pKa = 10.09 EE212 pKa = 4.36 GKK214 pKa = 10.35 KK215 pKa = 9.48 PLTIHH220 pKa = 6.8 GPTIPTSKK228 pKa = 11.05 LEE230 pKa = 4.24 GACVTKK236 pKa = 10.65 FCGTKK241 pKa = 10.29 GLRR244 pKa = 11.84 LSSGFFIIPPASWIKK259 pKa = 10.42 VFKK262 pKa = 9.99 TDD264 pKa = 3.34 CTDD267 pKa = 3.06 GVSIRR272 pKa = 11.84 GITWEE277 pKa = 4.44 SVVQDD282 pKa = 3.03 TVMYY286 pKa = 10.84 NEE288 pKa = 4.14 ISVIRR293 pKa = 11.84 LHH295 pKa = 6.58 CLNTIATMSALGRR308 pKa = 11.84 ASSVQLGIFQPWTPGIHH325 pKa = 5.33 PVYY328 pKa = 10.28 RR329 pKa = 11.84 VGVDD333 pKa = 3.04 NHH335 pKa = 7.18 LEE337 pKa = 4.2 TKK339 pKa = 8.31 MCGYY343 pKa = 10.24 ILGEE347 pKa = 4.43 GIDD350 pKa = 3.9 MNEE353 pKa = 4.54 DD354 pKa = 3.02 GAIGKK359 pKa = 8.75 DD360 pKa = 3.49 HH361 pKa = 7.27 LGHH364 pKa = 6.94 LVFWQDD370 pKa = 2.46 WVSPRR375 pKa = 11.84 KK376 pKa = 9.72 LSCEE380 pKa = 3.71 AYY382 pKa = 9.8 GPNGIYY388 pKa = 9.78 RR389 pKa = 11.84 DD390 pKa = 4.06 EE391 pKa = 4.47 KK392 pKa = 10.89 CQLHH396 pKa = 5.66 YY397 pKa = 10.4 PWIEE401 pKa = 3.62 LRR403 pKa = 11.84 QDD405 pKa = 2.61 IMHH408 pKa = 7.02 RR409 pKa = 11.84 SILVQQTLQEE419 pKa = 4.4 APHH422 pKa = 5.71 VTEE425 pKa = 3.51 QHH427 pKa = 5.51 YY428 pKa = 10.81 SQTEE432 pKa = 4.21 LEE434 pKa = 4.41 SDD436 pKa = 3.61 FQLFDD441 pKa = 4.81 KK442 pKa = 11.05 DD443 pKa = 3.69 FTDD446 pKa = 3.58 TKK448 pKa = 10.08 DD449 pKa = 2.93 IEE451 pKa = 4.22 MAIFSWTSSVRR462 pKa = 11.84 LGLAIGGVVLVIIVGLVLLVKK483 pKa = 10.51 LGLIAYY489 pKa = 8.59 IWDD492 pKa = 3.38 KK493 pKa = 10.56 CCRR496 pKa = 11.84 RR497 pKa = 11.84 KK498 pKa = 8.0 QHH500 pKa = 5.54 RR501 pKa = 11.84 QEE503 pKa = 4.71 SIHH506 pKa = 6.88 LEE508 pKa = 4.06 PLNPIYY514 pKa = 10.88 SS515 pKa = 3.6
Molecular weight: 57.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.922
IPC2_protein 5.995
IPC_protein 6.071
Toseland 6.338
ProMoST 6.465
Dawson 6.415
Bjellqvist 6.415
Wikipedia 6.44
Rodwell 6.415
Grimsley 6.453
Solomon 6.415
Lehninger 6.415
Nozaki 6.707
DTASelect 6.868
Thurlkill 6.898
EMBOSS 6.868
Sillero 6.81
Patrickios 3.694
IPC_peptide 6.44
IPC2_peptide 6.737
IPC2.peptide.svr19 6.694
Protein with the highest isoelectric point:
>tr|A0A1L3KND6|A0A1L3KND6_9VIRU Putative glycoprotein OS=Xinzhou dimarhabdovirus virus 1 OX=1923768 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.42 RR3 pKa = 11.84 ILSKK7 pKa = 10.64 SWWTSKK13 pKa = 9.9 SHH15 pKa = 4.26 SHH17 pKa = 6.07 EE18 pKa = 4.19 MGEE21 pKa = 4.5 SSGSHH26 pKa = 6.97 PEE28 pKa = 4.12 SQSVPVSWDD37 pKa = 2.84 QGVALTEE44 pKa = 4.35 PPSAPLSSSGPFCLDD59 pKa = 3.58 LEE61 pKa = 4.71 VEE63 pKa = 4.72 AYY65 pKa = 10.82 LHH67 pKa = 5.23 VQCNRR72 pKa = 11.84 GVSVKK77 pKa = 9.58 TLSQHH82 pKa = 5.55 ISQYY86 pKa = 9.8 RR87 pKa = 11.84 DD88 pKa = 2.95 AYY90 pKa = 10.27 QGEE93 pKa = 4.28 LGLYY97 pKa = 8.48 GVNSIVLCLALSGVNKK113 pKa = 10.27 CQLTEE118 pKa = 3.72 SSAYY122 pKa = 8.43 HH123 pKa = 5.9 FKK125 pKa = 9.73 YY126 pKa = 9.04 TSEE129 pKa = 3.85 IATVCRR135 pKa = 11.84 FFTQSDD141 pKa = 3.44 KK142 pKa = 11.27 TILFRR147 pKa = 11.84 YY148 pKa = 8.49 GRR150 pKa = 11.84 VVSKK154 pKa = 10.32 SAWSSLGIGHH164 pKa = 6.2 KK165 pKa = 8.5 TNWVFSVSLKK175 pKa = 7.64 PTRR178 pKa = 11.84 VSGLDD183 pKa = 3.7 YY184 pKa = 10.82 EE185 pKa = 4.47 KK186 pKa = 10.64 HH187 pKa = 5.49 APKK190 pKa = 10.6 AFAEE194 pKa = 4.35 LFPLMEE200 pKa = 5.5 LNFSRR205 pKa = 11.84 DD206 pKa = 3.21 PEE208 pKa = 4.39 TNQIILVV215 pKa = 3.82
Molecular weight: 23.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.821
IPC2_protein 6.912
IPC_protein 7.059
Toseland 6.912
ProMoST 7.483
Dawson 7.644
Bjellqvist 7.688
Wikipedia 7.585
Rodwell 7.644
Grimsley 7.015
Solomon 7.746
Lehninger 7.761
Nozaki 7.995
DTASelect 7.819
Thurlkill 7.849
EMBOSS 7.922
Sillero 8.097
Patrickios 4.024
IPC_peptide 7.732
IPC2_peptide 7.337
IPC2.peptide.svr19 7.288
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2904
215
2174
968.0
110.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.616 ± 0.418
1.86 ± 0.639
4.511 ± 0.488
6.267 ± 0.391
4.408 ± 0.352
5.82 ± 0.723
2.927 ± 0.345
7.025 ± 1.031
5.131 ± 0.249
10.572 ± 0.497
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.376 ± 0.357
4.787 ± 1.241
4.545 ± 0.261
3.099 ± 0.338
4.959 ± 0.593
9.539 ± 1.595
5.992 ± 0.326
6.198 ± 0.843
2.135 ± 0.054
4.236 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here