Amino acid dipepetide frequency for Shigella phage vB_SflS-ISF001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.117AlaAla: 8.117 ± 1.007
1.15AlaCys: 1.15 ± 0.321
4.262AlaAsp: 4.262 ± 0.408
5.344AlaGlu: 5.344 ± 0.656
2.029AlaPhe: 2.029 ± 0.367
5.615AlaGly: 5.615 ± 0.641
1.15AlaHis: 1.15 ± 0.232
6.156AlaIle: 6.156 ± 0.715
5.885AlaLys: 5.885 ± 1.15
6.088AlaLeu: 6.088 ± 0.718
2.706AlaMet: 2.706 ± 0.436
3.112AlaAsn: 3.112 ± 0.416
1.894AlaPro: 1.894 ± 0.409
3.45AlaGln: 3.45 ± 0.588
4.059AlaArg: 4.059 ± 0.449
5.276AlaSer: 5.276 ± 0.907
4.194AlaThr: 4.194 ± 0.594
5.276AlaVal: 5.276 ± 0.6
1.285AlaTrp: 1.285 ± 0.337
2.3AlaTyr: 2.3 ± 0.391
0.0AlaXaa: 0.0 ± 0.0
Cys
0.609CysAla: 0.609 ± 0.207
0.203CysCys: 0.203 ± 0.116
1.218CysAsp: 1.218 ± 0.306
1.082CysGlu: 1.082 ± 0.27
0.541CysPhe: 0.541 ± 0.21
1.15CysGly: 1.15 ± 0.324
0.271CysHis: 0.271 ± 0.137
1.082CysIle: 1.082 ± 0.264
0.879CysLys: 0.879 ± 0.349
0.879CysLeu: 0.879 ± 0.246
0.406CysMet: 0.406 ± 0.17
0.812CysAsn: 0.812 ± 0.261
0.474CysPro: 0.474 ± 0.19
0.474CysGln: 0.474 ± 0.169
0.676CysArg: 0.676 ± 0.234
0.744CysSer: 0.744 ± 0.193
0.744CysThr: 0.744 ± 0.255
1.015CysVal: 1.015 ± 0.294
0.203CysTrp: 0.203 ± 0.118
0.474CysTyr: 0.474 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
5.885AspAla: 5.885 ± 0.806
0.541AspCys: 0.541 ± 0.174
3.856AspAsp: 3.856 ± 0.651
4.532AspGlu: 4.532 ± 0.45
2.706AspPhe: 2.706 ± 0.505
6.02AspGly: 6.02 ± 0.835
1.218AspHis: 1.218 ± 0.292
3.112AspIle: 3.112 ± 0.351
3.856AspLys: 3.856 ± 0.435
3.653AspLeu: 3.653 ± 0.517
1.691AspMet: 1.691 ± 0.3
3.247AspAsn: 3.247 ± 0.606
2.029AspPro: 2.029 ± 0.302
2.029AspGln: 2.029 ± 0.421
2.3AspArg: 2.3 ± 0.45
4.397AspSer: 4.397 ± 0.485
2.638AspThr: 2.638 ± 0.403
4.735AspVal: 4.735 ± 0.578
0.676AspTrp: 0.676 ± 0.194
2.165AspTyr: 2.165 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
5.006GluAla: 5.006 ± 0.582
0.947GluCys: 0.947 ± 0.27
3.653GluAsp: 3.653 ± 0.471
4.397GluGlu: 4.397 ± 0.622
2.976GluPhe: 2.976 ± 0.52
3.044GluGly: 3.044 ± 0.42
0.947GluHis: 0.947 ± 0.25
6.359GluIle: 6.359 ± 0.647
5.073GluLys: 5.073 ± 0.79
4.532GluLeu: 4.532 ± 0.543
2.435GluMet: 2.435 ± 0.398
2.976GluAsn: 2.976 ± 0.451
1.285GluPro: 1.285 ± 0.419
2.503GluGln: 2.503 ± 0.42
3.382GluArg: 3.382 ± 0.617
3.382GluSer: 3.382 ± 0.447
3.653GluThr: 3.653 ± 0.561
5.547GluVal: 5.547 ± 0.826
0.947GluTrp: 0.947 ± 0.223
2.368GluTyr: 2.368 ± 0.491
0.0GluXaa: 0.0 ± 0.0
Phe
2.638PheAla: 2.638 ± 0.392
0.812PheCys: 0.812 ± 0.247
2.571PheAsp: 2.571 ± 0.407
2.841PheGlu: 2.841 ± 0.421
1.556PhePhe: 1.556 ± 0.394
3.247PheGly: 3.247 ± 0.435
0.676PheHis: 0.676 ± 0.28
2.773PheIle: 2.773 ± 0.382
2.503PheLys: 2.503 ± 0.441
1.759PheLeu: 1.759 ± 0.326
1.488PheMet: 1.488 ± 0.272
2.097PheAsn: 2.097 ± 0.374
1.826PhePro: 1.826 ± 0.402
1.285PheGln: 1.285 ± 0.355
1.623PheArg: 1.623 ± 0.295
2.097PheSer: 2.097 ± 0.408
2.638PheThr: 2.638 ± 0.409
2.368PheVal: 2.368 ± 0.337
0.676PheTrp: 0.676 ± 0.226
1.15PheTyr: 1.15 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
4.532GlyAla: 4.532 ± 0.791
1.421GlyCys: 1.421 ± 0.311
4.194GlyAsp: 4.194 ± 0.538
4.126GlyGlu: 4.126 ± 0.46
2.503GlyPhe: 2.503 ± 0.421
5.412GlyGly: 5.412 ± 0.67
1.082GlyHis: 1.082 ± 0.353
4.465GlyIle: 4.465 ± 0.547
5.885GlyLys: 5.885 ± 0.606
4.6GlyLeu: 4.6 ± 0.445
2.368GlyMet: 2.368 ± 0.508
3.72GlyAsn: 3.72 ± 0.651
0.879GlyPro: 0.879 ± 0.23
2.3GlyGln: 2.3 ± 0.388
3.112GlyArg: 3.112 ± 0.4
5.885GlySer: 5.885 ± 0.572
3.923GlyThr: 3.923 ± 0.471
6.494GlyVal: 6.494 ± 0.682
1.285GlyTrp: 1.285 ± 0.314
3.247GlyTyr: 3.247 ± 0.467
0.0GlyXaa: 0.0 ± 0.0
His
0.744HisAla: 0.744 ± 0.236
0.271HisCys: 0.271 ± 0.141
1.218HisAsp: 1.218 ± 0.273
1.421HisGlu: 1.421 ± 0.296
0.879HisPhe: 0.879 ± 0.203
1.353HisGly: 1.353 ± 0.366
0.676HisHis: 0.676 ± 0.224
1.421HisIle: 1.421 ± 0.37
1.421HisLys: 1.421 ± 0.381
0.947HisLeu: 0.947 ± 0.341
0.338HisMet: 0.338 ± 0.142
0.676HisAsn: 0.676 ± 0.241
0.474HisPro: 0.474 ± 0.193
0.541HisGln: 0.541 ± 0.179
1.218HisArg: 1.218 ± 0.352
0.879HisSer: 0.879 ± 0.253
1.082HisThr: 1.082 ± 0.325
1.082HisVal: 1.082 ± 0.285
0.271HisTrp: 0.271 ± 0.172
0.812HisTyr: 0.812 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
5.75IleAla: 5.75 ± 0.635
0.676IleCys: 0.676 ± 0.225
5.006IleAsp: 5.006 ± 0.562
5.276IleGlu: 5.276 ± 0.573
2.435IlePhe: 2.435 ± 0.474
4.803IleGly: 4.803 ± 0.605
0.879IleHis: 0.879 ± 0.253
4.6IleIle: 4.6 ± 0.676
4.803IleLys: 4.803 ± 0.565
3.585IleLeu: 3.585 ± 0.487
1.894IleMet: 1.894 ± 0.41
3.991IleAsn: 3.991 ± 0.583
2.706IlePro: 2.706 ± 0.484
2.435IleGln: 2.435 ± 0.383
3.044IleArg: 3.044 ± 0.348
5.141IleSer: 5.141 ± 0.575
5.615IleThr: 5.615 ± 0.631
4.668IleVal: 4.668 ± 0.603
0.947IleTrp: 0.947 ± 0.258
2.232IleTyr: 2.232 ± 0.342
0.0IleXaa: 0.0 ± 0.0
Lys
6.291LysAla: 6.291 ± 1.018
0.879LysCys: 0.879 ± 0.31
3.653LysAsp: 3.653 ± 0.546
5.073LysGlu: 5.073 ± 0.481
2.706LysPhe: 2.706 ± 0.537
4.194LysGly: 4.194 ± 0.582
1.556LysHis: 1.556 ± 0.388
4.803LysIle: 4.803 ± 0.621
4.397LysLys: 4.397 ± 0.592
5.141LysLeu: 5.141 ± 0.579
2.773LysMet: 2.773 ± 0.432
3.044LysAsn: 3.044 ± 0.467
3.179LysPro: 3.179 ± 0.518
2.909LysGln: 2.909 ± 0.575
3.45LysArg: 3.45 ± 0.582
4.126LysSer: 4.126 ± 0.52
3.179LysThr: 3.179 ± 0.384
5.073LysVal: 5.073 ± 0.626
0.609LysTrp: 0.609 ± 0.214
1.894LysTyr: 1.894 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
5.344LeuAla: 5.344 ± 0.815
0.879LeuCys: 0.879 ± 0.235
3.991LeuAsp: 3.991 ± 0.462
3.382LeuGlu: 3.382 ± 0.509
1.691LeuPhe: 1.691 ± 0.325
3.518LeuGly: 3.518 ± 0.494
1.353LeuHis: 1.353 ± 0.361
4.735LeuIle: 4.735 ± 0.619
5.209LeuLys: 5.209 ± 0.713
5.006LeuLeu: 5.006 ± 0.548
1.691LeuMet: 1.691 ± 0.259
2.976LeuAsn: 2.976 ± 0.444
3.179LeuPro: 3.179 ± 0.462
2.029LeuGln: 2.029 ± 0.303
3.382LeuArg: 3.382 ± 0.423
5.073LeuSer: 5.073 ± 0.522
4.803LeuThr: 4.803 ± 0.513
4.059LeuVal: 4.059 ± 0.524
1.082LeuTrp: 1.082 ± 0.317
2.097LeuTyr: 2.097 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
2.165MetAla: 2.165 ± 0.368
0.406MetCys: 0.406 ± 0.15
1.285MetAsp: 1.285 ± 0.251
2.029MetGlu: 2.029 ± 0.352
1.353MetPhe: 1.353 ± 0.321
1.082MetGly: 1.082 ± 0.284
0.609MetHis: 0.609 ± 0.232
2.909MetIle: 2.909 ± 0.41
2.571MetLys: 2.571 ± 0.415
2.165MetLeu: 2.165 ± 0.416
1.691MetMet: 1.691 ± 0.329
1.353MetAsn: 1.353 ± 0.326
1.082MetPro: 1.082 ± 0.242
1.015MetGln: 1.015 ± 0.267
1.894MetArg: 1.894 ± 0.33
2.909MetSer: 2.909 ± 0.491
1.488MetThr: 1.488 ± 0.321
2.029MetVal: 2.029 ± 0.397
0.203MetTrp: 0.203 ± 0.104
0.744MetTyr: 0.744 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
3.72AsnAla: 3.72 ± 0.535
0.474AsnCys: 0.474 ± 0.173
3.45AsnAsp: 3.45 ± 0.514
2.909AsnGlu: 2.909 ± 0.448
2.232AsnPhe: 2.232 ± 0.367
4.329AsnGly: 4.329 ± 0.691
0.541AsnHis: 0.541 ± 0.207
3.585AsnIle: 3.585 ± 0.401
2.841AsnLys: 2.841 ± 0.445
3.315AsnLeu: 3.315 ± 0.452
0.744AsnMet: 0.744 ± 0.201
2.503AsnAsn: 2.503 ± 0.463
1.894AsnPro: 1.894 ± 0.368
2.232AsnGln: 2.232 ± 0.46
2.097AsnArg: 2.097 ± 0.341
3.179AsnSer: 3.179 ± 0.471
2.3AsnThr: 2.3 ± 0.379
3.856AsnVal: 3.856 ± 0.586
0.947AsnTrp: 0.947 ± 0.272
1.691AsnTyr: 1.691 ± 0.338
0.0AsnXaa: 0.0 ± 0.0
Pro
2.773ProAla: 2.773 ± 0.482
0.541ProCys: 0.541 ± 0.184
2.232ProAsp: 2.232 ± 0.467
3.044ProGlu: 3.044 ± 0.498
1.556ProPhe: 1.556 ± 0.344
2.909ProGly: 2.909 ± 0.392
0.744ProHis: 0.744 ± 0.196
2.232ProIle: 2.232 ± 0.375
1.759ProLys: 1.759 ± 0.415
1.759ProLeu: 1.759 ± 0.252
0.879ProMet: 0.879 ± 0.234
2.232ProAsn: 2.232 ± 0.354
1.421ProPro: 1.421 ± 0.439
1.15ProGln: 1.15 ± 0.338
1.285ProArg: 1.285 ± 0.283
1.759ProSer: 1.759 ± 0.382
1.759ProThr: 1.759 ± 0.282
3.382ProVal: 3.382 ± 0.417
0.609ProTrp: 0.609 ± 0.214
1.894ProTyr: 1.894 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
3.247GlnAla: 3.247 ± 0.437
0.271GlnCys: 0.271 ± 0.133
1.826GlnAsp: 1.826 ± 0.366
2.3GlnGlu: 2.3 ± 0.343
1.353GlnPhe: 1.353 ± 0.286
2.435GlnGly: 2.435 ± 0.387
0.541GlnHis: 0.541 ± 0.163
2.976GlnIle: 2.976 ± 0.427
1.962GlnLys: 1.962 ± 0.391
3.45GlnLeu: 3.45 ± 0.545
1.421GlnMet: 1.421 ± 0.293
1.691GlnAsn: 1.691 ± 0.34
1.353GlnPro: 1.353 ± 0.377
1.691GlnGln: 1.691 ± 0.45
1.894GlnArg: 1.894 ± 0.435
2.638GlnSer: 2.638 ± 0.527
1.353GlnThr: 1.353 ± 0.301
2.571GlnVal: 2.571 ± 0.381
0.812GlnTrp: 0.812 ± 0.231
1.488GlnTyr: 1.488 ± 0.318
0.0GlnXaa: 0.0 ± 0.0
Arg
3.585ArgAla: 3.585 ± 0.518
0.744ArgCys: 0.744 ± 0.343
2.841ArgAsp: 2.841 ± 0.477
3.382ArgGlu: 3.382 ± 0.468
2.3ArgPhe: 2.3 ± 0.413
3.382ArgGly: 3.382 ± 0.434
0.879ArgHis: 0.879 ± 0.22
3.044ArgIle: 3.044 ± 0.414
3.518ArgLys: 3.518 ± 0.412
3.315ArgLeu: 3.315 ± 0.442
1.285ArgMet: 1.285 ± 0.285
2.232ArgAsn: 2.232 ± 0.496
2.232ArgPro: 2.232 ± 0.414
1.218ArgGln: 1.218 ± 0.358
2.841ArgArg: 2.841 ± 0.53
2.976ArgSer: 2.976 ± 0.384
1.759ArgThr: 1.759 ± 0.389
4.126ArgVal: 4.126 ± 0.471
0.744ArgTrp: 0.744 ± 0.239
2.029ArgTyr: 2.029 ± 0.375
0.0ArgXaa: 0.0 ± 0.0
Ser
5.75SerAla: 5.75 ± 0.632
0.879SerCys: 0.879 ± 0.26
4.329SerAsp: 4.329 ± 0.605
4.059SerGlu: 4.059 ± 0.525
2.773SerPhe: 2.773 ± 0.354
6.156SerGly: 6.156 ± 0.602
1.285SerHis: 1.285 ± 0.302
4.329SerIle: 4.329 ± 0.509
3.653SerLys: 3.653 ± 0.522
5.682SerLeu: 5.682 ± 0.652
1.759SerMet: 1.759 ± 0.3
2.435SerAsn: 2.435 ± 0.516
1.962SerPro: 1.962 ± 0.354
2.909SerGln: 2.909 ± 0.415
3.044SerArg: 3.044 ± 0.374
4.262SerSer: 4.262 ± 0.502
3.788SerThr: 3.788 ± 0.524
4.803SerVal: 4.803 ± 0.577
0.879SerTrp: 0.879 ± 0.272
2.029SerTyr: 2.029 ± 0.392
0.0SerXaa: 0.0 ± 0.0
Thr
4.194ThrAla: 4.194 ± 0.577
1.218ThrCys: 1.218 ± 0.285
3.247ThrAsp: 3.247 ± 0.46
3.247ThrGlu: 3.247 ± 0.495
2.435ThrPhe: 2.435 ± 0.418
5.073ThrGly: 5.073 ± 0.512
1.082ThrHis: 1.082 ± 0.37
3.991ThrIle: 3.991 ± 0.411
3.247ThrLys: 3.247 ± 0.454
3.247ThrLeu: 3.247 ± 0.367
1.556ThrMet: 1.556 ± 0.346
2.3ThrAsn: 2.3 ± 0.412
3.382ThrPro: 3.382 ± 0.567
2.435ThrGln: 2.435 ± 0.424
2.097ThrArg: 2.097 ± 0.4
3.72ThrSer: 3.72 ± 0.492
2.976ThrThr: 2.976 ± 0.431
3.653ThrVal: 3.653 ± 0.58
0.676ThrTrp: 0.676 ± 0.216
2.097ThrTyr: 2.097 ± 0.399
0.0ThrXaa: 0.0 ± 0.0
Val
4.87ValAla: 4.87 ± 0.579
0.744ValCys: 0.744 ± 0.218
4.735ValAsp: 4.735 ± 0.636
3.991ValGlu: 3.991 ± 0.571
3.112ValPhe: 3.112 ± 0.492
4.126ValGly: 4.126 ± 0.659
1.556ValHis: 1.556 ± 0.324
4.532ValIle: 4.532 ± 0.535
6.359ValLys: 6.359 ± 0.683
3.72ValLeu: 3.72 ± 0.501
2.029ValMet: 2.029 ± 0.345
4.465ValAsn: 4.465 ± 0.504
2.976ValPro: 2.976 ± 0.471
2.638ValGln: 2.638 ± 0.49
4.126ValArg: 4.126 ± 0.508
5.276ValSer: 5.276 ± 0.659
4.6ValThr: 4.6 ± 0.566
5.615ValVal: 5.615 ± 0.707
1.285ValTrp: 1.285 ± 0.238
2.503ValTyr: 2.503 ± 0.404
0.0ValXaa: 0.0 ± 0.0
Trp
1.15TrpAla: 1.15 ± 0.318
0.406TrpCys: 0.406 ± 0.199
1.015TrpAsp: 1.015 ± 0.274
0.812TrpGlu: 0.812 ± 0.277
0.338TrpPhe: 0.338 ± 0.22
0.947TrpGly: 0.947 ± 0.214
0.271TrpHis: 0.271 ± 0.124
0.676TrpIle: 0.676 ± 0.201
0.812TrpLys: 0.812 ± 0.233
0.947TrpLeu: 0.947 ± 0.265
0.474TrpMet: 0.474 ± 0.174
0.744TrpAsn: 0.744 ± 0.218
0.406TrpPro: 0.406 ± 0.197
0.406TrpGln: 0.406 ± 0.136
1.015TrpArg: 1.015 ± 0.245
1.285TrpSer: 1.285 ± 0.28
1.15TrpThr: 1.15 ± 0.346
0.879TrpVal: 0.879 ± 0.212
0.203TrpTrp: 0.203 ± 0.102
0.609TrpTyr: 0.609 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.976TyrAla: 2.976 ± 0.458
0.474TyrCys: 0.474 ± 0.188
2.706TyrAsp: 2.706 ± 0.414
2.165TyrGlu: 2.165 ± 0.29
1.15TyrPhe: 1.15 ± 0.334
2.638TyrGly: 2.638 ± 0.386
0.406TyrHis: 0.406 ± 0.169
2.706TyrIle: 2.706 ± 0.509
2.3TyrLys: 2.3 ± 0.387
1.691TyrLeu: 1.691 ± 0.328
1.285TyrMet: 1.285 ± 0.303
2.232TyrAsn: 2.232 ± 0.366
1.285TyrPro: 1.285 ± 0.282
1.623TyrGln: 1.623 ± 0.308
1.894TyrArg: 1.894 ± 0.302
1.826TyrSer: 1.826 ± 0.366
2.232TyrThr: 2.232 ± 0.421
2.029TyrVal: 2.029 ± 0.525
0.203TyrTrp: 0.203 ± 0.13
1.623TyrTyr: 1.623 ± 0.302
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (14784 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski