Acinetobacter phage Loki

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lokivirus; Acinetobacter virus Loki

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1KM07|A0A0P1KM07_9CAUD Putative head-tail joining protein OS=Acinetobacter phage Loki OX=1970374 GN=LOKI_08 PE=4 SV=1
MM1 pKa = 7.22GHH3 pKa = 5.85YY4 pKa = 10.06KK5 pKa = 8.87ITVSHH10 pKa = 5.2TVEE13 pKa = 4.1NEE15 pKa = 3.89AEE17 pKa = 4.17EE18 pKa = 4.32TAVDD22 pKa = 3.72YY23 pKa = 10.74QVIMVGYY30 pKa = 9.09CGNDD34 pKa = 3.2GGQSDD39 pKa = 4.07IEE41 pKa = 4.72GEE43 pKa = 4.07TEE45 pKa = 4.07YY46 pKa = 11.38YY47 pKa = 9.92IDD49 pKa = 4.79DD50 pKa = 4.03EE51 pKa = 4.28QVEE54 pKa = 4.68DD55 pKa = 5.06FDD57 pKa = 4.39TLPAEE62 pKa = 4.69VKK64 pKa = 10.61ALLWDD69 pKa = 4.01MEE71 pKa = 4.26LHH73 pKa = 7.29DD74 pKa = 4.43YY75 pKa = 11.53AKK77 pKa = 10.69GDD79 pKa = 3.41YY80 pKa = 9.31HH81 pKa = 7.05VKK83 pKa = 10.47HH84 pKa = 6.52EE85 pKa = 4.43EE86 pKa = 3.78PQPYY90 pKa = 10.09NEE92 pKa = 3.99WDD94 pKa = 3.12

Molecular weight: 10.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1KJW2|A0A0P1KJW2_9CAUD Putative head-tail joining protein OS=Acinetobacter phage Loki OX=1970374 GN=LOKI_09 PE=4 SV=1
MM1 pKa = 7.62SKK3 pKa = 9.59IQLIDD8 pKa = 3.41DD9 pKa = 3.66VRR11 pKa = 11.84RR12 pKa = 11.84IWKK15 pKa = 9.33FISVWLIIVAGFVQSIFIVAPDD37 pKa = 5.15AILTTWNLLPQEE49 pKa = 4.94LKK51 pKa = 10.27DD52 pKa = 4.19TIPPQYY58 pKa = 10.29VQFITVIILVFAIVGRR74 pKa = 11.84AIKK77 pKa = 10.0QKK79 pKa = 10.56RR80 pKa = 3.3

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13233

38

1161

259.5

28.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.446 ± 0.825

0.952 ± 0.146

5.925 ± 0.256

6.008 ± 0.294

3.99 ± 0.198

6.93 ± 0.358

1.36 ± 0.163

6.098 ± 0.266

6.355 ± 0.375

7.927 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.15

5.758 ± 0.241

3.862 ± 0.283

4.255 ± 0.385

4.141 ± 0.194

5.909 ± 0.227

6.446 ± 0.337

6.786 ± 0.253

1.504 ± 0.149

3.884 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski