Amino acid dipepetide frequency for Streptomyces phage WheeHeim

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.241AlaAla: 12.241 ± 2.012
0.54AlaCys: 0.54 ± 0.302
4.86AlaAsp: 4.86 ± 0.934
5.041AlaGlu: 5.041 ± 1.18
1.98AlaPhe: 1.98 ± 0.735
8.461AlaGly: 8.461 ± 1.472
1.8AlaHis: 1.8 ± 0.471
4.86AlaIle: 4.86 ± 0.872
7.201AlaLys: 7.201 ± 1.267
7.021AlaLeu: 7.021 ± 1.17
3.6AlaMet: 3.6 ± 0.834
5.041AlaAsn: 5.041 ± 0.762
4.86AlaPro: 4.86 ± 1.318
4.32AlaGln: 4.32 ± 0.768
3.24AlaArg: 3.24 ± 0.75
5.041AlaSer: 5.041 ± 0.899
7.381AlaThr: 7.381 ± 1.533
5.581AlaVal: 5.581 ± 1.236
1.62AlaTrp: 1.62 ± 0.458
3.24AlaTyr: 3.24 ± 0.686
0.0AlaXaa: 0.0 ± 0.0
Cys
0.36CysAla: 0.36 ± 0.267
0.0CysCys: 0.0 ± 0.0
0.36CysAsp: 0.36 ± 0.259
0.18CysGlu: 0.18 ± 0.171
0.0CysPhe: 0.0 ± 0.0
0.9CysGly: 0.9 ± 0.383
0.54CysHis: 0.54 ± 0.303
0.0CysIle: 0.0 ± 0.0
0.36CysLys: 0.36 ± 0.209
0.36CysLeu: 0.36 ± 0.219
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.36CysPro: 0.36 ± 0.255
0.18CysGln: 0.18 ± 0.198
0.72CysArg: 0.72 ± 0.383
0.0CysSer: 0.0 ± 0.0
0.36CysThr: 0.36 ± 0.23
0.72CysVal: 0.72 ± 0.288
0.0CysTrp: 0.0 ± 0.0
0.18CysTyr: 0.18 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
5.941AspAla: 5.941 ± 0.965
0.18AspCys: 0.18 ± 0.134
3.42AspAsp: 3.42 ± 0.911
3.6AspGlu: 3.6 ± 1.27
3.24AspPhe: 3.24 ± 0.625
4.68AspGly: 4.68 ± 0.709
0.72AspHis: 0.72 ± 0.324
2.88AspIle: 2.88 ± 0.581
3.42AspLys: 3.42 ± 0.957
3.06AspLeu: 3.06 ± 0.754
1.44AspMet: 1.44 ± 0.403
2.34AspAsn: 2.34 ± 0.647
4.14AspPro: 4.14 ± 0.829
3.24AspGln: 3.24 ± 0.859
1.98AspArg: 1.98 ± 0.596
2.88AspSer: 2.88 ± 0.694
3.6AspThr: 3.6 ± 0.71
4.14AspVal: 4.14 ± 0.773
0.72AspTrp: 0.72 ± 0.324
0.72AspTyr: 0.72 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
3.06GluAla: 3.06 ± 0.934
0.36GluCys: 0.36 ± 0.227
3.06GluAsp: 3.06 ± 0.784
2.34GluGlu: 2.34 ± 0.666
3.78GluPhe: 3.78 ± 0.791
2.7GluGly: 2.7 ± 0.552
0.72GluHis: 0.72 ± 0.379
3.42GluIle: 3.42 ± 0.913
3.6GluLys: 3.6 ± 0.814
3.24GluLeu: 3.24 ± 0.782
1.62GluMet: 1.62 ± 0.498
3.42GluAsn: 3.42 ± 0.732
2.34GluPro: 2.34 ± 0.602
0.9GluGln: 0.9 ± 0.352
1.98GluArg: 1.98 ± 0.678
4.68GluSer: 4.68 ± 1.057
2.16GluThr: 2.16 ± 0.579
3.78GluVal: 3.78 ± 0.833
1.26GluTrp: 1.26 ± 0.439
1.8GluTyr: 1.8 ± 0.685
0.0GluXaa: 0.0 ± 0.0
Phe
3.06PheAla: 3.06 ± 0.599
0.0PheCys: 0.0 ± 0.0
1.98PheAsp: 1.98 ± 0.688
2.16PheGlu: 2.16 ± 0.614
1.08PhePhe: 1.08 ± 0.408
4.32PheGly: 4.32 ± 0.731
0.9PheHis: 0.9 ± 0.363
1.26PheIle: 1.26 ± 0.422
0.9PheLys: 0.9 ± 0.352
2.7PheLeu: 2.7 ± 0.713
0.36PheMet: 0.36 ± 0.188
2.34PheAsn: 2.34 ± 0.616
1.62PhePro: 1.62 ± 0.523
1.62PheGln: 1.62 ± 0.621
1.44PheArg: 1.44 ± 0.669
2.34PheSer: 2.34 ± 0.585
2.34PheThr: 2.34 ± 0.736
2.7PheVal: 2.7 ± 0.702
0.72PheTrp: 0.72 ± 0.332
0.9PheTyr: 0.9 ± 0.402
0.0PheXaa: 0.0 ± 0.0
Gly
7.201GlyAla: 7.201 ± 1.212
0.36GlyCys: 0.36 ± 0.268
5.761GlyAsp: 5.761 ± 1.32
4.68GlyGlu: 4.68 ± 0.726
4.14GlyPhe: 4.14 ± 0.808
9.541GlyGly: 9.541 ± 1.505
0.72GlyHis: 0.72 ± 0.381
4.5GlyIle: 4.5 ± 0.89
7.201GlyLys: 7.201 ± 1.861
5.581GlyLeu: 5.581 ± 1.036
1.62GlyMet: 1.62 ± 0.616
7.561GlyAsn: 7.561 ± 1.786
5.401GlyPro: 5.401 ± 0.996
3.06GlyGln: 3.06 ± 0.622
3.96GlyArg: 3.96 ± 0.876
6.481GlySer: 6.481 ± 1.393
6.481GlyThr: 6.481 ± 0.937
4.68GlyVal: 4.68 ± 0.997
2.7GlyTrp: 2.7 ± 0.65
2.52GlyTyr: 2.52 ± 0.625
0.0GlyXaa: 0.0 ± 0.0
His
1.08HisAla: 1.08 ± 0.387
0.0HisCys: 0.0 ± 0.0
1.08HisAsp: 1.08 ± 0.554
0.9HisGlu: 0.9 ± 0.408
0.72HisPhe: 0.72 ± 0.401
1.98HisGly: 1.98 ± 0.8
0.36HisHis: 0.36 ± 0.254
0.72HisIle: 0.72 ± 0.315
0.72HisLys: 0.72 ± 0.348
1.44HisLeu: 1.44 ± 0.523
0.18HisMet: 0.18 ± 0.176
0.36HisAsn: 0.36 ± 0.254
1.62HisPro: 1.62 ± 0.54
0.54HisGln: 0.54 ± 0.283
1.08HisArg: 1.08 ± 0.525
0.18HisSer: 0.18 ± 0.179
0.72HisThr: 0.72 ± 0.351
1.44HisVal: 1.44 ± 0.512
0.36HisTrp: 0.36 ± 0.222
0.54HisTyr: 0.54 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
4.86IleAla: 4.86 ± 0.936
0.36IleCys: 0.36 ± 0.246
1.98IleAsp: 1.98 ± 0.601
1.62IleGlu: 1.62 ± 0.512
1.08IlePhe: 1.08 ± 0.406
5.401IleGly: 5.401 ± 1.063
1.26IleHis: 1.26 ± 0.377
1.98IleIle: 1.98 ± 0.601
1.98IleLys: 1.98 ± 0.657
4.86IleLeu: 4.86 ± 0.905
1.08IleMet: 1.08 ± 0.352
1.8IleAsn: 1.8 ± 0.683
1.98IlePro: 1.98 ± 0.535
1.44IleGln: 1.44 ± 0.49
4.5IleArg: 4.5 ± 0.837
4.14IleSer: 4.14 ± 0.784
4.32IleThr: 4.32 ± 0.823
3.6IleVal: 3.6 ± 0.728
1.08IleTrp: 1.08 ± 0.414
1.8IleTyr: 1.8 ± 0.65
0.0IleXaa: 0.0 ± 0.0
Lys
4.32LysAla: 4.32 ± 1.527
0.0LysCys: 0.0 ± 0.0
3.42LysAsp: 3.42 ± 0.885
2.88LysGlu: 2.88 ± 0.766
1.26LysPhe: 1.26 ± 0.5
4.32LysGly: 4.32 ± 1.076
0.9LysHis: 0.9 ± 0.344
3.24LysIle: 3.24 ± 0.939
2.88LysLys: 2.88 ± 0.788
3.06LysLeu: 3.06 ± 0.879
3.24LysMet: 3.24 ± 0.716
1.98LysAsn: 1.98 ± 0.493
2.88LysPro: 2.88 ± 0.884
1.8LysGln: 1.8 ± 0.58
2.7LysArg: 2.7 ± 0.858
3.24LysSer: 3.24 ± 0.679
3.78LysThr: 3.78 ± 0.683
4.14LysVal: 4.14 ± 0.886
1.8LysTrp: 1.8 ± 0.62
0.54LysTyr: 0.54 ± 0.33
0.0LysXaa: 0.0 ± 0.0
Leu
7.201LeuAla: 7.201 ± 1.009
0.36LeuCys: 0.36 ± 0.265
2.88LeuAsp: 2.88 ± 0.746
4.14LeuGlu: 4.14 ± 0.928
1.08LeuPhe: 1.08 ± 0.378
7.021LeuGly: 7.021 ± 1.013
1.8LeuHis: 1.8 ± 0.568
5.221LeuIle: 5.221 ± 0.998
3.06LeuLys: 3.06 ± 0.853
4.5LeuLeu: 4.5 ± 0.992
1.98LeuMet: 1.98 ± 0.508
3.42LeuAsn: 3.42 ± 0.626
3.42LeuPro: 3.42 ± 1.02
1.44LeuGln: 1.44 ± 0.609
4.5LeuArg: 4.5 ± 0.831
4.14LeuSer: 4.14 ± 0.764
3.6LeuThr: 3.6 ± 0.852
4.86LeuVal: 4.86 ± 1.136
1.62LeuTrp: 1.62 ± 0.551
1.98LeuTyr: 1.98 ± 0.66
0.0LeuXaa: 0.0 ± 0.0
Met
4.14MetAla: 4.14 ± 0.795
0.18MetCys: 0.18 ± 0.167
0.9MetAsp: 0.9 ± 0.323
0.36MetGlu: 0.36 ± 0.228
1.08MetPhe: 1.08 ± 0.472
2.88MetGly: 2.88 ± 0.744
0.36MetHis: 0.36 ± 0.244
0.9MetIle: 0.9 ± 0.361
1.08MetLys: 1.08 ± 0.465
3.42MetLeu: 3.42 ± 0.823
1.44MetMet: 1.44 ± 0.824
1.62MetAsn: 1.62 ± 0.509
2.52MetPro: 2.52 ± 0.683
0.18MetGln: 0.18 ± 0.179
1.26MetArg: 1.26 ± 0.532
2.34MetSer: 2.34 ± 0.682
1.08MetThr: 1.08 ± 0.412
1.44MetVal: 1.44 ± 0.571
0.36MetTrp: 0.36 ± 0.245
1.44MetTyr: 1.44 ± 0.326
0.0MetXaa: 0.0 ± 0.0
Asn
5.761AsnAla: 5.761 ± 1.052
0.18AsnCys: 0.18 ± 0.134
2.52AsnAsp: 2.52 ± 0.572
1.8AsnGlu: 1.8 ± 0.556
0.9AsnPhe: 0.9 ± 0.35
6.841AsnGly: 6.841 ± 1.144
0.72AsnHis: 0.72 ± 0.343
1.26AsnIle: 1.26 ± 0.504
2.52AsnLys: 2.52 ± 0.729
2.7AsnLeu: 2.7 ± 0.727
1.62AsnMet: 1.62 ± 0.66
1.26AsnAsn: 1.26 ± 0.52
5.221AsnPro: 5.221 ± 1.242
1.26AsnGln: 1.26 ± 0.434
1.8AsnArg: 1.8 ± 0.518
3.78AsnSer: 3.78 ± 0.693
4.14AsnThr: 4.14 ± 1.006
3.24AsnVal: 3.24 ± 0.611
0.72AsnTrp: 0.72 ± 0.295
1.26AsnTyr: 1.26 ± 0.339
0.0AsnXaa: 0.0 ± 0.0
Pro
6.481ProAla: 6.481 ± 1.98
0.36ProCys: 0.36 ± 0.268
5.221ProAsp: 5.221 ± 1.12
3.6ProGlu: 3.6 ± 0.834
2.52ProPhe: 2.52 ± 0.596
4.5ProGly: 4.5 ± 0.995
0.72ProHis: 0.72 ± 0.348
2.34ProIle: 2.34 ± 0.548
3.78ProLys: 3.78 ± 0.853
2.52ProLeu: 2.52 ± 0.67
1.98ProMet: 1.98 ± 0.615
3.24ProAsn: 3.24 ± 0.821
3.78ProPro: 3.78 ± 1.229
1.26ProGln: 1.26 ± 0.428
2.16ProArg: 2.16 ± 0.667
2.34ProSer: 2.34 ± 0.586
4.32ProThr: 4.32 ± 1.059
4.32ProVal: 4.32 ± 1.049
1.26ProTrp: 1.26 ± 0.526
2.16ProTyr: 2.16 ± 0.797
0.0ProXaa: 0.0 ± 0.0
Gln
3.6GlnAla: 3.6 ± 0.632
0.36GlnCys: 0.36 ± 0.326
1.62GlnAsp: 1.62 ± 0.589
1.8GlnGlu: 1.8 ± 0.546
1.26GlnPhe: 1.26 ± 0.715
2.7GlnGly: 2.7 ± 0.759
0.36GlnHis: 0.36 ± 0.35
1.98GlnIle: 1.98 ± 0.941
0.9GlnLys: 0.9 ± 0.375
1.98GlnLeu: 1.98 ± 0.596
1.08GlnMet: 1.08 ± 0.391
1.8GlnAsn: 1.8 ± 0.639
1.44GlnPro: 1.44 ± 0.647
1.8GlnGln: 1.8 ± 0.623
1.8GlnArg: 1.8 ± 0.836
1.62GlnSer: 1.62 ± 0.405
2.52GlnThr: 2.52 ± 0.764
2.16GlnVal: 2.16 ± 0.455
1.08GlnTrp: 1.08 ± 0.52
2.16GlnTyr: 2.16 ± 0.679
0.0GlnXaa: 0.0 ± 0.0
Arg
4.5ArgAla: 4.5 ± 0.805
0.72ArgCys: 0.72 ± 0.35
2.88ArgAsp: 2.88 ± 0.84
3.42ArgGlu: 3.42 ± 0.869
1.26ArgPhe: 1.26 ± 0.532
3.96ArgGly: 3.96 ± 0.71
0.54ArgHis: 0.54 ± 0.345
3.78ArgIle: 3.78 ± 0.913
4.14ArgLys: 4.14 ± 0.986
3.96ArgLeu: 3.96 ± 0.969
1.8ArgMet: 1.8 ± 0.477
2.52ArgAsn: 2.52 ± 0.68
2.34ArgPro: 2.34 ± 0.657
1.8ArgGln: 1.8 ± 0.698
3.06ArgArg: 3.06 ± 0.601
2.16ArgSer: 2.16 ± 0.519
2.34ArgThr: 2.34 ± 0.602
4.32ArgVal: 4.32 ± 1.007
0.36ArgTrp: 0.36 ± 0.247
1.26ArgTyr: 1.26 ± 0.426
0.0ArgXaa: 0.0 ± 0.0
Ser
7.921SerAla: 7.921 ± 0.751
0.72SerCys: 0.72 ± 0.419
3.42SerAsp: 3.42 ± 0.817
2.7SerGlu: 2.7 ± 0.728
1.26SerPhe: 1.26 ± 0.412
7.561SerGly: 7.561 ± 1.494
0.54SerHis: 0.54 ± 0.268
2.88SerIle: 2.88 ± 0.782
1.26SerLys: 1.26 ± 0.492
3.78SerLeu: 3.78 ± 0.867
2.52SerMet: 2.52 ± 0.733
2.88SerAsn: 2.88 ± 0.809
3.24SerPro: 3.24 ± 0.859
2.7SerGln: 2.7 ± 0.838
2.52SerArg: 2.52 ± 0.577
4.14SerSer: 4.14 ± 0.981
5.041SerThr: 5.041 ± 1.142
4.5SerVal: 4.5 ± 0.729
0.54SerTrp: 0.54 ± 0.329
1.98SerTyr: 1.98 ± 0.477
0.0SerXaa: 0.0 ± 0.0
Thr
6.841ThrAla: 6.841 ± 1.344
0.54ThrCys: 0.54 ± 0.265
2.7ThrAsp: 2.7 ± 0.776
2.52ThrGlu: 2.52 ± 0.681
2.34ThrPhe: 2.34 ± 0.492
5.581ThrGly: 5.581 ± 1.151
1.62ThrHis: 1.62 ± 0.507
3.6ThrIle: 3.6 ± 1.054
3.06ThrLys: 3.06 ± 0.654
4.68ThrLeu: 4.68 ± 1.144
0.72ThrMet: 0.72 ± 0.274
2.7ThrAsn: 2.7 ± 0.706
4.86ThrPro: 4.86 ± 1.308
3.24ThrGln: 3.24 ± 0.905
3.96ThrArg: 3.96 ± 1.001
5.221ThrSer: 5.221 ± 0.815
5.401ThrThr: 5.401 ± 0.966
5.401ThrVal: 5.401 ± 1.088
0.72ThrTrp: 0.72 ± 0.314
2.7ThrTyr: 2.7 ± 0.721
0.0ThrXaa: 0.0 ± 0.0
Val
6.121ValAla: 6.121 ± 0.995
0.18ValCys: 0.18 ± 0.171
3.6ValAsp: 3.6 ± 0.742
3.06ValGlu: 3.06 ± 0.652
3.42ValPhe: 3.42 ± 0.876
7.561ValGly: 7.561 ± 1.352
0.9ValHis: 0.9 ± 0.353
4.32ValIle: 4.32 ± 0.924
3.78ValLys: 3.78 ± 0.741
4.68ValLeu: 4.68 ± 1.001
1.62ValMet: 1.62 ± 0.62
2.52ValAsn: 2.52 ± 0.769
4.14ValPro: 4.14 ± 0.9
1.62ValGln: 1.62 ± 0.412
3.96ValArg: 3.96 ± 0.873
4.68ValSer: 4.68 ± 0.819
5.941ValThr: 5.941 ± 0.884
5.761ValVal: 5.761 ± 1.11
1.44ValTrp: 1.44 ± 0.548
1.44ValTyr: 1.44 ± 0.573
0.0ValXaa: 0.0 ± 0.0
Trp
1.26TrpAla: 1.26 ± 0.481
0.18TrpCys: 0.18 ± 0.198
2.88TrpAsp: 2.88 ± 0.668
0.9TrpGlu: 0.9 ± 0.354
1.08TrpPhe: 1.08 ± 0.387
0.72TrpGly: 0.72 ± 0.372
0.18TrpHis: 0.18 ± 0.171
0.72TrpIle: 0.72 ± 0.36
0.18TrpLys: 0.18 ± 0.191
1.26TrpLeu: 1.26 ± 0.34
0.36TrpMet: 0.36 ± 0.268
0.9TrpAsn: 0.9 ± 0.411
0.9TrpPro: 0.9 ± 0.324
0.54TrpGln: 0.54 ± 0.282
1.62TrpArg: 1.62 ± 0.448
1.26TrpSer: 1.26 ± 0.521
0.9TrpThr: 0.9 ± 0.449
1.98TrpVal: 1.98 ± 0.698
0.18TrpTrp: 0.18 ± 0.191
1.08TrpTyr: 1.08 ± 0.48
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.98TyrAla: 1.98 ± 0.482
0.0TyrCys: 0.0 ± 0.0
1.8TyrAsp: 1.8 ± 0.604
2.52TyrGlu: 2.52 ± 0.719
1.26TyrPhe: 1.26 ± 0.415
2.52TyrGly: 2.52 ± 0.644
0.36TyrHis: 0.36 ± 0.259
1.26TyrIle: 1.26 ± 0.507
0.54TyrLys: 0.54 ± 0.249
3.42TyrLeu: 3.42 ± 0.782
0.54TyrMet: 0.54 ± 0.243
1.8TyrAsn: 1.8 ± 0.426
1.8TyrPro: 1.8 ± 0.761
1.08TyrGln: 1.08 ± 0.395
2.88TyrArg: 2.88 ± 0.983
1.44TyrSer: 1.44 ± 0.622
1.98TyrThr: 1.98 ± 0.508
1.98TyrVal: 1.98 ± 0.456
0.54TyrTrp: 0.54 ± 0.402
0.72TyrTyr: 0.72 ± 0.411
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (5556 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski