Thiohalocapsa marina
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3686 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5M8FKG4|A0A5M8FKG4_9GAMM Ribosomal RNA small subunit methyltransferase E OS=Thiohalocapsa marina OX=424902 GN=F2Q65_08725 PE=3 SV=1
MM1 pKa = 7.37 NKK3 pKa = 9.96 PYY5 pKa = 9.52 RR6 pKa = 11.84 TQPNSNRR13 pKa = 11.84 SSKK16 pKa = 10.05 PSAWMSLALGLSISLMSSASFAAPFGYY43 pKa = 10.62 AIDD46 pKa = 4.71 GDD48 pKa = 4.28 SLAPSVYY55 pKa = 10.52 EE56 pKa = 5.16 IDD58 pKa = 3.97 FADD61 pKa = 3.65 NSISEE66 pKa = 4.36 IGTAVTTDD74 pKa = 3.83 LPHH77 pKa = 6.9 QVDD80 pKa = 3.87 EE81 pKa = 4.81 VEE83 pKa = 4.58 ASSFGPGGVTLFAVTDD99 pKa = 4.13 DD100 pKa = 3.85 NSYY103 pKa = 11.36 KK104 pKa = 10.12 PGHH107 pKa = 7.12 DD108 pKa = 3.46 IDD110 pKa = 6.51 DD111 pKa = 4.72 DD112 pKa = 4.46 VDD114 pKa = 3.82 GTILTDD120 pKa = 3.82 ALYY123 pKa = 9.72 TINTTTGAATWIADD137 pKa = 3.99 LSRR140 pKa = 11.84 AIDD143 pKa = 3.87 DD144 pKa = 4.74 PGLAFCSSDD153 pKa = 2.9 QTMYY157 pKa = 11.12 LSSTVTGGGGALCARR172 pKa = 11.84 PEE174 pKa = 4.03 YY175 pKa = 10.97 GRR177 pKa = 11.84 TEE179 pKa = 3.73
Molecular weight: 18.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.846
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.834
Sillero 3.973
Patrickios 1.926
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A5M8FU65|A0A5M8FU65_9GAMM 50S ribosomal protein L13 OS=Thiohalocapsa marina OX=424902 GN=rplM PE=3 SV=1
MM1 pKa = 7.31 KK2 pKa = 9.5 QTFKK6 pKa = 10.9 PSRR9 pKa = 11.84 LKK11 pKa = 10.5 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 6.41 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 CATRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 9.36 VIQARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.12 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTPP44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3686
0
3686
1237040
31
5300
335.6
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.146 ± 0.056
1.001 ± 0.014
6.034 ± 0.027
5.718 ± 0.031
3.342 ± 0.024
8.291 ± 0.057
2.273 ± 0.019
4.539 ± 0.027
2.353 ± 0.03
11.618 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.173 ± 0.021
2.268 ± 0.021
5.507 ± 0.031
4.039 ± 0.029
8.047 ± 0.045
5.028 ± 0.028
4.846 ± 0.037
7.036 ± 0.039
1.418 ± 0.018
2.324 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here