Sinomonas humi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Sinomonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B2ALR5|A0A0B2ALR5_9MICC Tyr recombinase domain-containing protein OS=Sinomonas humi OX=1338436 GN=LK10_05995 PE=4 SV=1
MM1 pKa = 7.49AKK3 pKa = 9.84IAPEE7 pKa = 3.75CRR9 pKa = 11.84YY10 pKa = 10.08SEE12 pKa = 4.05EE13 pKa = 4.52HH14 pKa = 6.0EE15 pKa = 4.41WVATRR20 pKa = 11.84SDD22 pKa = 3.07SMAWVGVSAVATDD35 pKa = 3.57ALGEE39 pKa = 4.1IVYY42 pKa = 10.61LDD44 pKa = 3.72LPEE47 pKa = 5.59AGSEE51 pKa = 4.12VTAGSTCGEE60 pKa = 4.04IEE62 pKa = 4.25STKK65 pKa = 10.64SVSDD69 pKa = 4.02LYY71 pKa = 11.42SPVSGTVVEE80 pKa = 4.83VNQEE84 pKa = 4.38AIDD87 pKa = 4.04NPALVNEE94 pKa = 4.6DD95 pKa = 3.86PYY97 pKa = 11.2GAGWLFTVEE106 pKa = 4.31VTSEE110 pKa = 4.37GPLLSAEE117 pKa = 4.53EE118 pKa = 4.16YY119 pKa = 10.15ASKK122 pKa = 11.09NGGEE126 pKa = 4.13LL127 pKa = 3.41

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B2AFW3|A0A0B2AFW3_9MICC LysM domain-containing protein OS=Sinomonas humi OX=1338436 GN=LK10_18550 PE=4 SV=1
MM1 pKa = 7.53SGRR4 pKa = 11.84ARR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84WPRR13 pKa = 11.84SRR15 pKa = 11.84RR16 pKa = 11.84LRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84GRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84WRR26 pKa = 11.84CRR28 pKa = 11.84SRR30 pKa = 11.84TRR32 pKa = 11.84PRR34 pKa = 11.84GRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84GRR41 pKa = 11.84GTSVTGRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84PRR53 pKa = 11.84IRR55 pKa = 11.84PIPTVRR61 pKa = 11.84RR62 pKa = 3.68

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3444

0

3444

1076008

37

1635

312.4

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.344 ± 0.06

0.633 ± 0.011

5.502 ± 0.032

6.146 ± 0.049

3.153 ± 0.026

9.152 ± 0.037

2.1 ± 0.02

4.156 ± 0.028

2.422 ± 0.029

10.252 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.867 ± 0.017

2.172 ± 0.023

5.47 ± 0.029

3.026 ± 0.027

7.184 ± 0.042

5.846 ± 0.032

5.49 ± 0.032

8.565 ± 0.039

1.452 ± 0.02

2.067 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski