Beihai weivirus-like virus 5
Average proteome isoelectric point is 8.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL21|A0A1L3KL21_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 5 OX=1922753 PE=4 SV=1
MM1 pKa = 7.62 LPLRR5 pKa = 11.84 SGNCDD10 pKa = 2.82 LTLRR14 pKa = 11.84 DD15 pKa = 3.77 VPHH18 pKa = 6.44 YY19 pKa = 10.49 LQAQAGTRR27 pKa = 11.84 KK28 pKa = 9.57 GAWEE32 pKa = 3.81 ITVYY36 pKa = 10.58 EE37 pKa = 4.88 HH38 pKa = 6.81 DD39 pKa = 4.43 AEE41 pKa = 4.53 WFKK44 pKa = 11.23 SWRR47 pKa = 11.84 PNAYY51 pKa = 9.18 SVRR54 pKa = 11.84 FTEE57 pKa = 5.17 DD58 pKa = 3.07 EE59 pKa = 4.06 YY60 pKa = 11.89 SKK62 pKa = 11.07 LSRR65 pKa = 11.84 TMYY68 pKa = 10.01 HH69 pKa = 6.74 GLRR72 pKa = 11.84 NGTKK76 pKa = 9.03 LTSLYY81 pKa = 10.87 AGLEE85 pKa = 4.12 NASQSLWPTGSLDD98 pKa = 3.59 HH99 pKa = 6.72 VAMRR103 pKa = 11.84 HH104 pKa = 4.78 MGFPTVWRR112 pKa = 11.84 YY113 pKa = 7.62 ITNQTPARR121 pKa = 11.84 FEE123 pKa = 4.11 FAHH126 pKa = 6.95 KK127 pKa = 10.55 LGTYY131 pKa = 10.11 LVAPQSLLVPWWMITKK147 pKa = 10.33 KK148 pKa = 8.94 MRR150 pKa = 11.84 RR151 pKa = 11.84 RR152 pKa = 11.84 YY153 pKa = 9.46 AVAAFLVLAGLALKK167 pKa = 10.29 LRR169 pKa = 11.84 RR170 pKa = 11.84 IGRR173 pKa = 11.84 LALPRR178 pKa = 11.84 LTRR181 pKa = 11.84 VTGQSGGQEE190 pKa = 3.91 AKK192 pKa = 10.34 KK193 pKa = 10.41 DD194 pKa = 3.79 DD195 pKa = 4.28 PSGGKK200 pKa = 9.37 PPTPNEE206 pKa = 4.3 GGPKK210 pKa = 10.5 DD211 pKa = 4.47 GDD213 pKa = 3.82 TTPDD217 pKa = 3.2 AGNVKK222 pKa = 10.42 PEE224 pKa = 4.39 GGNNDD229 pKa = 4.62 DD230 pKa = 4.61 PPAPPMTTLRR240 pKa = 11.84 RR241 pKa = 11.84 TDD243 pKa = 3.03 TSFVIGGPPVMDD255 pKa = 4.55 EE256 pKa = 4.05 PALPGDD262 pKa = 4.25 FEE264 pKa = 4.48 NAAEE268 pKa = 4.02 AQEE271 pKa = 4.43 DD272 pKa = 3.91 GRR274 pKa = 11.84 TVVRR278 pKa = 11.84 GDD280 pKa = 3.33 VVAVLGQYY288 pKa = 9.86 FDD290 pKa = 3.62 KK291 pKa = 10.87 DD292 pKa = 3.31 RR293 pKa = 11.84 PINHH297 pKa = 5.95 MPIVGCMVGPCQLKK311 pKa = 9.35 PNVYY315 pKa = 10.56 AKK317 pKa = 9.73 TVFNLWAAIVKK328 pKa = 10.05 RR329 pKa = 11.84 IRR331 pKa = 11.84 EE332 pKa = 4.0 KK333 pKa = 10.82 ALKK336 pKa = 9.34 PKK338 pKa = 10.74 LSGEE342 pKa = 3.95 EE343 pKa = 3.64 KK344 pKa = 9.98 TRR346 pKa = 11.84 IGRR349 pKa = 11.84 LISKK353 pKa = 10.19 CMTPDD358 pKa = 2.94 KK359 pKa = 10.63 RR360 pKa = 11.84 CGVFAKK366 pKa = 10.39 HH367 pKa = 6.69 RR368 pKa = 11.84 IEE370 pKa = 4.1 EE371 pKa = 3.95 WAIAHH376 pKa = 6.94 FDD378 pKa = 4.02 LEE380 pKa = 4.5 EE381 pKa = 4.4 CKK383 pKa = 10.52 SGKK386 pKa = 9.21 WSIEE390 pKa = 3.74 RR391 pKa = 11.84 FRR393 pKa = 11.84 GSLEE397 pKa = 3.48 NLYY400 pKa = 10.87 AKK402 pKa = 8.4 EE403 pKa = 4.19 HH404 pKa = 5.94 PTFSFKK410 pKa = 11.16 ADD412 pKa = 3.24 VKK414 pKa = 9.61 YY415 pKa = 11.13 EE416 pKa = 3.95 CMPEE420 pKa = 4.02 GKK422 pKa = 10.12 APRR425 pKa = 11.84 MLIADD430 pKa = 3.91 GDD432 pKa = 4.11 EE433 pKa = 4.41 GQLMALAVVKK443 pKa = 10.57 CFEE446 pKa = 4.38 EE447 pKa = 4.42 LLFSHH452 pKa = 7.42 FEE454 pKa = 4.18 TKK456 pKa = 10.23 SIKK459 pKa = 10.31 HH460 pKa = 5.14 LAKK463 pKa = 9.81 RR464 pKa = 11.84 DD465 pKa = 3.9 AIDD468 pKa = 3.58 RR469 pKa = 11.84 VLKK472 pKa = 9.98 EE473 pKa = 4.0 LRR475 pKa = 11.84 APGAKK480 pKa = 9.58 AVEE483 pKa = 4.6 GDD485 pKa = 3.29 GSAWDD490 pKa = 3.89 TTCNVLIRR498 pKa = 11.84 GLVEE502 pKa = 4.04 NPVLRR507 pKa = 11.84 HH508 pKa = 5.04 ITTVLCNFGVIPSTWMEE525 pKa = 3.72 EE526 pKa = 3.91 HH527 pKa = 6.49 QRR529 pKa = 11.84 ACEE532 pKa = 3.94 KK533 pKa = 9.79 KK534 pKa = 7.57 TLRR537 pKa = 11.84 LFFSNKK543 pKa = 9.0 FEE545 pKa = 4.26 TMSTSIDD552 pKa = 3.56 AIRR555 pKa = 11.84 RR556 pKa = 11.84 SGHH559 pKa = 6.55 RR560 pKa = 11.84 GTSCLNWWINFVLWVSSVFKK580 pKa = 10.52 EE581 pKa = 4.05 PEE583 pKa = 3.76 RR584 pKa = 11.84 FLDD587 pKa = 3.49 VAVRR591 pKa = 11.84 NGTDD595 pKa = 3.05 LTGRR599 pKa = 11.84 SRR601 pKa = 11.84 WWNGCFEE608 pKa = 5.17 GDD610 pKa = 5.07 DD611 pKa = 4.13 SLCTMRR617 pKa = 11.84 PPMVEE622 pKa = 4.14 GDD624 pKa = 3.87 ALCQVFLAFWKK635 pKa = 10.68 SAGFNMKK642 pKa = 9.66 IVFCKK647 pKa = 9.9 TRR649 pKa = 11.84 ATFVGWHH656 pKa = 5.7 VGCTDD661 pKa = 3.47 GEE663 pKa = 4.59 LNNFRR668 pKa = 11.84 CPEE671 pKa = 4.08 LPRR674 pKa = 11.84 ALANSGVSVSPEE686 pKa = 3.89 AIKK689 pKa = 10.38 AAKK692 pKa = 10.11 DD693 pKa = 3.51 MNRR696 pKa = 11.84 SAVNVLAAASALARR710 pKa = 11.84 ASDD713 pKa = 3.79 FAGILPSVSVKK724 pKa = 10.91 YY725 pKa = 10.01 MDD727 pKa = 3.77 FAEE730 pKa = 4.59 SVSRR734 pKa = 11.84 TDD736 pKa = 3.68 FSDD739 pKa = 3.82 RR740 pKa = 11.84 EE741 pKa = 3.78 MSIRR745 pKa = 11.84 AFGEE749 pKa = 4.07 DD750 pKa = 3.51 GFSANAVRR758 pKa = 11.84 TQIMEE763 pKa = 4.17 RR764 pKa = 11.84 NIGITPDD771 pKa = 3.47 EE772 pKa = 4.5 EE773 pKa = 4.77 LVTMAALGYY782 pKa = 10.41 AATQDD787 pKa = 3.44 EE788 pKa = 5.21 VATFMEE794 pKa = 5.13 YY795 pKa = 10.52 GWSLDD800 pKa = 3.34 PHH802 pKa = 6.74 VLLDD806 pKa = 3.58 YY807 pKa = 10.78 EE808 pKa = 4.49 AFRR811 pKa = 11.84 GSLPPSWRR819 pKa = 11.84 AA820 pKa = 2.98
Molecular weight: 91.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.081
IPC2_protein 7.512
IPC_protein 7.322
Toseland 6.985
ProMoST 7.951
Dawson 8.039
Bjellqvist 8.478
Wikipedia 7.951
Rodwell 8.068
Grimsley 7.044
Solomon 8.083
Lehninger 8.112
Nozaki 8.77
DTASelect 8.185
Thurlkill 8.244
EMBOSS 8.258
Sillero 8.595
Patrickios 4.609
IPC_peptide 8.083
IPC2_peptide 7.468
IPC2.peptide.svr19 7.503
Protein with the highest isoelectric point:
>tr|A0A1L3KL21|A0A1L3KL21_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 5 OX=1922753 PE=4 SV=1
MM1 pKa = 7.55 AKK3 pKa = 9.94 GRR5 pKa = 11.84 SSLKK9 pKa = 10.02 PVTQARR15 pKa = 11.84 RR16 pKa = 11.84 WNRR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 10.55 GGGVTRR27 pKa = 11.84 ATAPRR32 pKa = 11.84 VLAQGACAVPKK43 pKa = 10.3 KK44 pKa = 10.92 AFGAGKK50 pKa = 9.95 NPNGWKK56 pKa = 10.21 KK57 pKa = 11.2 AMTLGLNARR66 pKa = 11.84 LPYY69 pKa = 10.31 HH70 pKa = 6.99 LGLPRR75 pKa = 11.84 PVGPYY80 pKa = 9.52 QVIRR84 pKa = 11.84 TTKK87 pKa = 9.29 VHH89 pKa = 4.94 TTKK92 pKa = 10.42 SGCVIFTPLQRR103 pKa = 11.84 RR104 pKa = 11.84 HH105 pKa = 6.63 DD106 pKa = 4.24 SLPGEE111 pKa = 4.29 PRR113 pKa = 11.84 WYY115 pKa = 9.88 AACGMEE121 pKa = 4.27 AVNTGVSINAANNATMISMPMDD143 pKa = 3.68 VLGSACDD150 pKa = 3.67 VVPAAMSVQVMCPTALQNASGAFTMARR177 pKa = 11.84 VNQQLTFGGDD187 pKa = 3.29 TRR189 pKa = 11.84 PWGQFITQFNSFFRR203 pKa = 11.84 PRR205 pKa = 11.84 LLTGGKK211 pKa = 8.68 LALRR215 pKa = 11.84 GVHH218 pKa = 6.33 ANAYY222 pKa = 7.79 PLDD225 pKa = 3.76 MSEE228 pKa = 4.24 YY229 pKa = 8.35 AHH231 pKa = 6.52 FAGVVPSLGTTTWNAITQSATRR253 pKa = 11.84 LPAVV257 pKa = 3.62
Molecular weight: 27.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.955
IPC_protein 10.979
Toseland 11.111
ProMoST 11.125
Dawson 11.155
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.286
Grimsley 11.199
Solomon 11.418
Lehninger 11.359
Nozaki 11.096
DTASelect 10.965
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.111
Patrickios 10.994
IPC_peptide 11.418
IPC2_peptide 10.277
IPC2.peptide.svr19 8.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1077
257
820
538.5
59.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.935 ± 1.277
2.136 ± 0.096
4.643 ± 1.369
5.2 ± 2.046
4.457 ± 0.485
8.078 ± 0.837
2.043 ± 0.049
3.343 ± 0.512
5.292 ± 0.514
8.078 ± 0.348
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.25 ± 0.325
3.993 ± 0.541
6.128 ± 0.642
2.414 ± 0.947
7.242 ± 0.076
5.757 ± 0.354
6.592 ± 0.999
6.964 ± 0.415
2.414 ± 0.238
2.043 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here