Salmonella phage IME207

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1W257|A0A1B1W257_9CAUD Putative transcriptional regulator OS=Salmonella phage IME207 OX=1873985 PE=4 SV=1
MM1 pKa = 7.06EE2 pKa = 4.67MEE4 pKa = 4.87WINVNDD10 pKa = 4.37EE11 pKa = 4.27LPEE14 pKa = 4.41SKK16 pKa = 10.38DD17 pKa = 3.55DD18 pKa = 3.88SVLVCSVDD26 pKa = 3.1GSKK29 pKa = 11.16YY30 pKa = 10.26NEE32 pKa = 3.93NGFPEE37 pKa = 4.71GGIDD41 pKa = 3.3MVHH44 pKa = 6.01IQDD47 pKa = 3.95YY48 pKa = 10.69FDD50 pKa = 6.61DD51 pKa = 3.76ITAGLDD57 pKa = 3.23EE58 pKa = 5.53KK59 pKa = 11.49GNQLYY64 pKa = 7.93TKK66 pKa = 9.63RR67 pKa = 11.84YY68 pKa = 7.78IEE70 pKa = 3.98MGITHH75 pKa = 7.23WMYY78 pKa = 11.03LPEE81 pKa = 5.76LPFCGEE87 pKa = 4.0KK88 pKa = 10.59

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1W260|A0A1B1W260_9CAUD Uncharacterized protein OS=Salmonella phage IME207 OX=1873985 PE=4 SV=1
MM1 pKa = 7.76GSTNSPSRR9 pKa = 11.84SRR11 pKa = 11.84ATGNTKK17 pKa = 9.72TGGKK21 pKa = 7.78TGAVKK26 pKa = 10.54PNGSTRR32 pKa = 11.84SPSRR36 pKa = 11.84GKK38 pKa = 10.36KK39 pKa = 9.35

Molecular weight: 3.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

14662

37

976

156.0

17.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.403 ± 0.467

1.323 ± 0.169

6.159 ± 0.239

6.452 ± 0.32

3.574 ± 0.16

7.073 ± 0.252

1.882 ± 0.167

6.445 ± 0.19

6.322 ± 0.358

7.045 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.246 ± 0.213

4.958 ± 0.221

3.615 ± 0.227

3.86 ± 0.261

5.422 ± 0.229

6.377 ± 0.245

5.518 ± 0.37

6.561 ± 0.256

1.794 ± 0.127

3.969 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski