Amino acid dipepetide frequency for Flavobacterium phage FCV-10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.136AlaAla: 2.136 ± 0.68
0.551AlaCys: 0.551 ± 0.18
2.618AlaAsp: 2.618 ± 0.377
3.376AlaGlu: 3.376 ± 0.58
2.618AlaPhe: 2.618 ± 0.48
2.962AlaGly: 2.962 ± 0.583
0.344AlaHis: 0.344 ± 0.157
3.858AlaIle: 3.858 ± 0.517
4.96AlaLys: 4.96 ± 0.877
3.789AlaLeu: 3.789 ± 0.858
1.171AlaMet: 1.171 ± 0.345
3.927AlaAsn: 3.927 ± 0.659
1.516AlaPro: 1.516 ± 0.327
1.722AlaGln: 1.722 ± 0.375
1.998AlaArg: 1.998 ± 0.433
4.064AlaSer: 4.064 ± 0.668
3.651AlaThr: 3.651 ± 0.593
3.307AlaVal: 3.307 ± 0.585
0.62AlaTrp: 0.62 ± 0.196
2.136AlaTyr: 2.136 ± 0.435
0.0AlaXaa: 0.0 ± 0.0
Cys
0.413CysAla: 0.413 ± 0.207
0.069CysCys: 0.069 ± 0.058
0.758CysAsp: 0.758 ± 0.209
0.413CysGlu: 0.413 ± 0.165
0.62CysPhe: 0.62 ± 0.217
0.344CysGly: 0.344 ± 0.145
0.207CysHis: 0.207 ± 0.129
0.62CysIle: 0.62 ± 0.209
1.102CysLys: 1.102 ± 0.332
0.896CysLeu: 0.896 ± 0.305
0.207CysMet: 0.207 ± 0.11
0.551CysAsn: 0.551 ± 0.22
0.207CysPro: 0.207 ± 0.116
0.207CysGln: 0.207 ± 0.117
0.413CysArg: 0.413 ± 0.195
0.482CysSer: 0.482 ± 0.171
0.827CysThr: 0.827 ± 0.219
0.62CysVal: 0.62 ± 0.208
0.0CysTrp: 0.0 ± 0.0
0.344CysTyr: 0.344 ± 0.156
0.0CysXaa: 0.0 ± 0.0
Asp
4.064AspAla: 4.064 ± 0.511
0.62AspCys: 0.62 ± 0.239
2.687AspAsp: 2.687 ± 0.511
3.996AspGlu: 3.996 ± 0.485
3.789AspPhe: 3.789 ± 0.485
3.238AspGly: 3.238 ± 0.382
0.62AspHis: 0.62 ± 0.218
3.996AspIle: 3.996 ± 0.411
3.789AspLys: 3.789 ± 0.47
7.233AspLeu: 7.233 ± 0.619
0.827AspMet: 0.827 ± 0.243
4.34AspAsn: 4.34 ± 0.632
1.653AspPro: 1.653 ± 0.343
1.309AspGln: 1.309 ± 0.331
1.378AspArg: 1.378 ± 0.347
3.996AspSer: 3.996 ± 0.649
2.342AspThr: 2.342 ± 0.37
3.789AspVal: 3.789 ± 0.553
0.827AspTrp: 0.827 ± 0.291
3.031AspTyr: 3.031 ± 0.487
0.0AspXaa: 0.0 ± 0.0
Glu
3.307GluAla: 3.307 ± 0.55
0.413GluCys: 0.413 ± 0.193
2.756GluAsp: 2.756 ± 0.412
4.547GluGlu: 4.547 ± 0.817
3.582GluPhe: 3.582 ± 0.497
2.067GluGly: 2.067 ± 0.474
1.102GluHis: 1.102 ± 0.278
6.062GluIle: 6.062 ± 0.588
7.44GluLys: 7.44 ± 1.081
7.922GluLeu: 7.922 ± 0.876
2.687GluMet: 2.687 ± 0.447
5.787GluAsn: 5.787 ± 0.909
1.516GluPro: 1.516 ± 0.353
2.48GluGln: 2.48 ± 0.477
3.169GluArg: 3.169 ± 0.675
4.684GluSer: 4.684 ± 0.592
4.133GluThr: 4.133 ± 0.543
3.238GluVal: 3.238 ± 0.414
0.758GluTrp: 0.758 ± 0.242
2.756GluTyr: 2.756 ± 0.463
0.0GluXaa: 0.0 ± 0.0
Phe
1.791PheAla: 1.791 ± 0.351
0.689PheCys: 0.689 ± 0.245
4.064PheAsp: 4.064 ± 0.539
3.789PheGlu: 3.789 ± 0.446
1.86PhePhe: 1.86 ± 0.35
3.513PheGly: 3.513 ± 0.541
0.413PheHis: 0.413 ± 0.14
3.72PheIle: 3.72 ± 0.526
5.098PheLys: 5.098 ± 0.574
3.651PheLeu: 3.651 ± 0.455
1.171PheMet: 1.171 ± 0.286
3.582PheAsn: 3.582 ± 0.5
1.102PhePro: 1.102 ± 0.24
1.309PheGln: 1.309 ± 0.261
1.447PheArg: 1.447 ± 0.283
2.48PheSer: 2.48 ± 0.48
2.824PheThr: 2.824 ± 0.466
2.756PheVal: 2.756 ± 0.419
0.551PheTrp: 0.551 ± 0.168
2.067PheTyr: 2.067 ± 0.395
0.0PheXaa: 0.0 ± 0.0
Gly
2.962GlyAla: 2.962 ± 0.567
0.344GlyCys: 0.344 ± 0.138
3.72GlyAsp: 3.72 ± 0.563
3.444GlyGlu: 3.444 ± 0.47
3.307GlyPhe: 3.307 ± 0.415
4.409GlyGly: 4.409 ± 0.631
0.207GlyHis: 0.207 ± 0.112
4.478GlyIle: 4.478 ± 0.514
5.029GlyLys: 5.029 ± 0.714
4.616GlyLeu: 4.616 ± 0.542
0.689GlyMet: 0.689 ± 0.192
3.307GlyAsn: 3.307 ± 0.381
0.551GlyPro: 0.551 ± 0.162
1.722GlyGln: 1.722 ± 0.31
1.86GlyArg: 1.86 ± 0.385
4.202GlySer: 4.202 ± 0.598
3.031GlyThr: 3.031 ± 0.531
3.927GlyVal: 3.927 ± 0.614
0.689GlyTrp: 0.689 ± 0.223
2.618GlyTyr: 2.618 ± 0.43
0.0GlyXaa: 0.0 ± 0.0
His
0.276HisAla: 0.276 ± 0.144
0.276HisCys: 0.276 ± 0.16
0.758HisAsp: 0.758 ± 0.209
0.896HisGlu: 0.896 ± 0.283
0.689HisPhe: 0.689 ± 0.194
0.964HisGly: 0.964 ± 0.355
0.069HisHis: 0.069 ± 0.07
1.24HisIle: 1.24 ± 0.335
1.171HisLys: 1.171 ± 0.274
0.758HisLeu: 0.758 ± 0.234
0.138HisMet: 0.138 ± 0.083
1.033HisAsn: 1.033 ± 0.335
0.689HisPro: 0.689 ± 0.183
0.62HisGln: 0.62 ± 0.18
0.827HisArg: 0.827 ± 0.22
0.689HisSer: 0.689 ± 0.207
0.413HisThr: 0.413 ± 0.157
0.482HisVal: 0.482 ± 0.201
0.413HisTrp: 0.413 ± 0.173
0.482HisTyr: 0.482 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
4.684IleAla: 4.684 ± 0.755
0.344IleCys: 0.344 ± 0.152
5.373IleAsp: 5.373 ± 0.562
6.407IleGlu: 6.407 ± 0.785
2.549IlePhe: 2.549 ± 0.398
4.34IleGly: 4.34 ± 0.533
1.171IleHis: 1.171 ± 0.275
6.131IleIle: 6.131 ± 0.712
7.578IleLys: 7.578 ± 0.788
6.407IleLeu: 6.407 ± 0.573
1.584IleMet: 1.584 ± 0.294
5.098IleAsn: 5.098 ± 0.6
2.756IlePro: 2.756 ± 0.494
4.064IleGln: 4.064 ± 0.426
2.549IleArg: 2.549 ± 0.404
5.236IleSer: 5.236 ± 0.679
4.684IleThr: 4.684 ± 0.582
4.34IleVal: 4.34 ± 0.593
0.689IleTrp: 0.689 ± 0.22
2.824IleTyr: 2.824 ± 0.487
0.0IleXaa: 0.0 ± 0.0
Lys
5.167LysAla: 5.167 ± 0.839
0.827LysCys: 0.827 ± 0.259
4.753LysAsp: 4.753 ± 0.509
7.165LysGlu: 7.165 ± 1.13
3.651LysPhe: 3.651 ± 0.447
5.787LysGly: 5.787 ± 0.629
1.447LysHis: 1.447 ± 0.295
6.476LysIle: 6.476 ± 0.603
8.267LysLys: 8.267 ± 0.824
8.749LysLeu: 8.749 ± 0.742
2.687LysMet: 2.687 ± 0.42
6.613LysAsn: 6.613 ± 0.695
3.444LysPro: 3.444 ± 0.523
3.789LysGln: 3.789 ± 0.659
4.34LysArg: 4.34 ± 0.623
6.2LysSer: 6.2 ± 0.597
5.649LysThr: 5.649 ± 0.629
4.409LysVal: 4.409 ± 0.497
1.171LysTrp: 1.171 ± 0.252
4.064LysTyr: 4.064 ± 0.651
0.0LysXaa: 0.0 ± 0.0
Leu
4.753LeuAla: 4.753 ± 0.566
0.689LeuCys: 0.689 ± 0.199
5.511LeuAsp: 5.511 ± 0.691
8.129LeuGlu: 8.129 ± 0.83
4.064LeuPhe: 4.064 ± 0.578
4.547LeuGly: 4.547 ± 0.551
1.033LeuHis: 1.033 ± 0.275
7.716LeuIle: 7.716 ± 0.821
8.68LeuLys: 8.68 ± 0.793
6.613LeuLeu: 6.613 ± 0.663
2.273LeuMet: 2.273 ± 0.44
5.993LeuAsn: 5.993 ± 0.762
3.169LeuPro: 3.169 ± 0.44
2.824LeuGln: 2.824 ± 0.39
3.376LeuArg: 3.376 ± 0.458
5.718LeuSer: 5.718 ± 0.649
5.58LeuThr: 5.58 ± 0.675
5.236LeuVal: 5.236 ± 0.552
0.896LeuTrp: 0.896 ± 0.247
2.756LeuTyr: 2.756 ± 0.453
0.0LeuXaa: 0.0 ± 0.0
Met
1.791MetAla: 1.791 ± 0.372
0.276MetCys: 0.276 ± 0.134
0.964MetAsp: 0.964 ± 0.364
0.964MetGlu: 0.964 ± 0.248
0.758MetPhe: 0.758 ± 0.208
1.102MetGly: 1.102 ± 0.273
0.138MetHis: 0.138 ± 0.103
2.067MetIle: 2.067 ± 0.427
2.342MetLys: 2.342 ± 0.392
1.378MetLeu: 1.378 ± 0.313
0.069MetMet: 0.069 ± 0.067
1.791MetAsn: 1.791 ± 0.429
0.62MetPro: 0.62 ± 0.254
1.447MetGln: 1.447 ± 0.281
1.171MetArg: 1.171 ± 0.349
1.516MetSer: 1.516 ± 0.353
1.033MetThr: 1.033 ± 0.268
0.964MetVal: 0.964 ± 0.293
0.276MetTrp: 0.276 ± 0.158
1.033MetTyr: 1.033 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
3.513AsnAla: 3.513 ± 0.595
0.896AsnCys: 0.896 ± 0.221
3.72AsnAsp: 3.72 ± 0.519
5.993AsnGlu: 5.993 ± 0.646
3.031AsnPhe: 3.031 ± 0.473
4.684AsnGly: 4.684 ± 0.777
1.102AsnHis: 1.102 ± 0.275
5.649AsnIle: 5.649 ± 0.555
6.682AsnLys: 6.682 ± 0.833
6.682AsnLeu: 6.682 ± 0.623
1.102AsnMet: 1.102 ± 0.258
6.407AsnAsn: 6.407 ± 0.744
2.136AsnPro: 2.136 ± 0.383
2.411AsnGln: 2.411 ± 0.443
2.136AsnArg: 2.136 ± 0.321
5.442AsnSer: 5.442 ± 0.598
4.409AsnThr: 4.409 ± 0.496
3.307AsnVal: 3.307 ± 0.533
0.413AsnTrp: 0.413 ± 0.159
3.238AsnTyr: 3.238 ± 0.577
0.0AsnXaa: 0.0 ± 0.0
Pro
1.309ProAla: 1.309 ± 0.353
0.344ProCys: 0.344 ± 0.15
2.411ProAsp: 2.411 ± 0.543
1.722ProGlu: 1.722 ± 0.356
1.653ProPhe: 1.653 ± 0.283
0.62ProGly: 0.62 ± 0.217
0.62ProHis: 0.62 ± 0.154
2.273ProIle: 2.273 ± 0.375
2.893ProLys: 2.893 ± 0.574
2.204ProLeu: 2.204 ± 0.468
0.207ProMet: 0.207 ± 0.175
2.342ProAsn: 2.342 ± 0.342
0.413ProPro: 0.413 ± 0.156
1.171ProGln: 1.171 ± 0.329
0.689ProArg: 0.689 ± 0.215
1.86ProSer: 1.86 ± 0.386
2.342ProThr: 2.342 ± 0.453
2.273ProVal: 2.273 ± 0.388
0.207ProTrp: 0.207 ± 0.119
1.378ProTyr: 1.378 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
1.929GlnAla: 1.929 ± 0.387
0.207GlnCys: 0.207 ± 0.125
1.447GlnAsp: 1.447 ± 0.286
2.756GlnGlu: 2.756 ± 0.465
1.722GlnPhe: 1.722 ± 0.283
2.136GlnGly: 2.136 ± 0.346
0.689GlnHis: 0.689 ± 0.314
2.756GlnIle: 2.756 ± 0.373
3.444GlnLys: 3.444 ± 0.611
3.927GlnLeu: 3.927 ± 0.489
1.033GlnMet: 1.033 ± 0.257
2.136GlnAsn: 2.136 ± 0.411
1.033GlnPro: 1.033 ± 0.346
1.86GlnGln: 1.86 ± 0.449
1.033GlnArg: 1.033 ± 0.243
2.824GlnSer: 2.824 ± 0.494
1.791GlnThr: 1.791 ± 0.287
1.791GlnVal: 1.791 ± 0.349
0.413GlnTrp: 0.413 ± 0.153
1.653GlnTyr: 1.653 ± 0.273
0.0GlnXaa: 0.0 ± 0.0
Arg
1.516ArgAla: 1.516 ± 0.327
0.413ArgCys: 0.413 ± 0.15
2.136ArgAsp: 2.136 ± 0.427
2.342ArgGlu: 2.342 ± 0.499
2.342ArgPhe: 2.342 ± 0.482
1.102ArgGly: 1.102 ± 0.232
0.62ArgHis: 0.62 ± 0.195
3.031ArgIle: 3.031 ± 0.385
3.651ArgLys: 3.651 ± 0.544
3.651ArgLeu: 3.651 ± 0.732
0.689ArgMet: 0.689 ± 0.214
2.136ArgAsn: 2.136 ± 0.406
0.551ArgPro: 0.551 ± 0.199
1.033ArgGln: 1.033 ± 0.277
1.102ArgArg: 1.102 ± 0.282
1.791ArgSer: 1.791 ± 0.327
2.273ArgThr: 2.273 ± 0.369
2.756ArgVal: 2.756 ± 0.356
0.207ArgTrp: 0.207 ± 0.118
1.791ArgTyr: 1.791 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
3.307SerAla: 3.307 ± 0.66
0.344SerCys: 0.344 ± 0.149
3.513SerAsp: 3.513 ± 0.375
4.064SerGlu: 4.064 ± 0.568
3.169SerPhe: 3.169 ± 0.475
4.271SerGly: 4.271 ± 0.534
0.827SerHis: 0.827 ± 0.219
5.649SerIle: 5.649 ± 0.623
7.785SerLys: 7.785 ± 0.727
6.062SerLeu: 6.062 ± 0.653
1.722SerMet: 1.722 ± 0.29
4.478SerAsn: 4.478 ± 0.693
1.309SerPro: 1.309 ± 0.308
2.618SerGln: 2.618 ± 0.421
1.86SerArg: 1.86 ± 0.377
2.756SerSer: 2.756 ± 0.528
3.789SerThr: 3.789 ± 0.543
4.684SerVal: 4.684 ± 0.54
0.758SerTrp: 0.758 ± 0.194
3.1SerTyr: 3.1 ± 0.477
0.0SerXaa: 0.0 ± 0.0
Thr
3.789ThrAla: 3.789 ± 0.793
0.482ThrCys: 0.482 ± 0.231
3.513ThrAsp: 3.513 ± 0.511
3.513ThrGlu: 3.513 ± 0.527
2.893ThrPhe: 2.893 ± 0.356
3.996ThrGly: 3.996 ± 0.517
0.758ThrHis: 0.758 ± 0.241
5.304ThrIle: 5.304 ± 0.548
4.133ThrLys: 4.133 ± 0.683
4.96ThrLeu: 4.96 ± 0.668
1.24ThrMet: 1.24 ± 0.387
4.34ThrAsn: 4.34 ± 0.574
2.756ThrPro: 2.756 ± 0.414
1.722ThrGln: 1.722 ± 0.353
1.86ThrArg: 1.86 ± 0.384
3.238ThrSer: 3.238 ± 0.45
3.1ThrThr: 3.1 ± 0.622
4.064ThrVal: 4.064 ± 0.748
0.896ThrTrp: 0.896 ± 0.229
2.273ThrTyr: 2.273 ± 0.391
0.0ThrXaa: 0.0 ± 0.0
Val
2.687ValAla: 2.687 ± 0.595
0.758ValCys: 0.758 ± 0.283
3.582ValAsp: 3.582 ± 0.459
3.996ValGlu: 3.996 ± 0.455
2.48ValPhe: 2.48 ± 0.359
2.342ValGly: 2.342 ± 0.376
0.689ValHis: 0.689 ± 0.229
4.616ValIle: 4.616 ± 0.611
5.098ValLys: 5.098 ± 0.635
5.098ValLeu: 5.098 ± 0.609
1.033ValMet: 1.033 ± 0.21
5.236ValAsn: 5.236 ± 0.594
1.378ValPro: 1.378 ± 0.326
1.86ValGln: 1.86 ± 0.337
2.48ValArg: 2.48 ± 0.425
4.34ValSer: 4.34 ± 0.582
3.72ValThr: 3.72 ± 0.47
3.238ValVal: 3.238 ± 0.421
0.482ValTrp: 0.482 ± 0.185
3.238ValTyr: 3.238 ± 0.47
0.0ValXaa: 0.0 ± 0.0
Trp
0.827TrpAla: 0.827 ± 0.245
0.138TrpCys: 0.138 ± 0.101
0.551TrpAsp: 0.551 ± 0.194
0.551TrpGlu: 0.551 ± 0.193
0.551TrpPhe: 0.551 ± 0.177
0.827TrpGly: 0.827 ± 0.227
0.138TrpHis: 0.138 ± 0.094
0.896TrpIle: 0.896 ± 0.332
1.171TrpLys: 1.171 ± 0.294
0.62TrpLeu: 0.62 ± 0.201
0.413TrpMet: 0.413 ± 0.189
0.689TrpAsn: 0.689 ± 0.204
0.138TrpPro: 0.138 ± 0.097
0.344TrpGln: 0.344 ± 0.175
0.276TrpArg: 0.276 ± 0.133
0.758TrpSer: 0.758 ± 0.252
0.758TrpThr: 0.758 ± 0.193
0.482TrpVal: 0.482 ± 0.19
0.138TrpTrp: 0.138 ± 0.105
0.62TrpTyr: 0.62 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.102TyrAla: 1.102 ± 0.215
0.62TyrCys: 0.62 ± 0.208
2.687TyrAsp: 2.687 ± 0.419
2.342TyrGlu: 2.342 ± 0.411
2.549TyrPhe: 2.549 ± 0.425
1.86TyrGly: 1.86 ± 0.395
0.689TyrHis: 0.689 ± 0.211
2.273TyrIle: 2.273 ± 0.381
4.409TyrLys: 4.409 ± 0.612
4.34TyrLeu: 4.34 ± 0.591
0.689TyrMet: 0.689 ± 0.207
3.169TyrAsn: 3.169 ± 0.57
1.998TyrPro: 1.998 ± 0.4
2.067TyrGln: 2.067 ± 0.449
1.171TyrArg: 1.171 ± 0.324
3.789TyrSer: 3.789 ± 0.581
2.48TyrThr: 2.48 ± 0.427
2.687TyrVal: 2.687 ± 0.468
0.482TyrTrp: 0.482 ± 0.178
1.722TyrTyr: 1.722 ± 0.424
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14517 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski