Paguma larvata circovirus
Average proteome isoelectric point is 8.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B8GVJ6|A0A3B8GVJ6_9CIRC ATP-dependent helicase Rep OS=Paguma larvata circovirus OX=2294094 PE=3 SV=1
MM1 pKa = 7.82 AEE3 pKa = 4.0 RR4 pKa = 11.84 VPPVRR9 pKa = 11.84 WCFTLNNWTQEE20 pKa = 3.91 EE21 pKa = 4.67 WDD23 pKa = 4.69 SITQVLGPTVKK34 pKa = 10.14 YY35 pKa = 10.17 LCVGKK40 pKa = 10.3 EE41 pKa = 3.77 VGKK44 pKa = 10.79 EE45 pKa = 4.14 GTQHH49 pKa = 5.54 LQGYY53 pKa = 8.04 MEE55 pKa = 4.45 LKK57 pKa = 9.53 KK58 pKa = 10.43 RR59 pKa = 11.84 KK60 pKa = 9.08 RR61 pKa = 11.84 LKK63 pKa = 9.77 QVKK66 pKa = 9.42 DD67 pKa = 3.41 LPGLQRR73 pKa = 11.84 AHH75 pKa = 6.95 LEE77 pKa = 3.9 AARR80 pKa = 11.84 GNSSQAAEE88 pKa = 4.17 YY89 pKa = 9.98 CKK91 pKa = 10.74 KK92 pKa = 10.63 EE93 pKa = 3.54 GDD95 pKa = 3.77 YY96 pKa = 11.6 LEE98 pKa = 5.47 FGTPGNEE105 pKa = 3.62 QGKK108 pKa = 8.03 RR109 pKa = 11.84 TDD111 pKa = 3.53 LNEE114 pKa = 3.64 ACRR117 pKa = 11.84 ILRR120 pKa = 11.84 EE121 pKa = 4.36 SNGNLRR127 pKa = 11.84 AVAEE131 pKa = 4.11 KK132 pKa = 10.77 QEE134 pKa = 4.14 AVFVKK139 pKa = 10.36 YY140 pKa = 10.24 FGGLQRR146 pKa = 11.84 YY147 pKa = 8.51 CYY149 pKa = 9.89 TMNLGGRR156 pKa = 11.84 RR157 pKa = 11.84 DD158 pKa = 3.85 FKK160 pKa = 10.95 TITTVICGPPGCGKK174 pKa = 8.21 TRR176 pKa = 11.84 YY177 pKa = 9.58 VFEE180 pKa = 4.57 KK181 pKa = 10.82 VGGDD185 pKa = 3.5 LGRR188 pKa = 11.84 VYY190 pKa = 10.08 MKK192 pKa = 10.55 PRR194 pKa = 11.84 GLWWDD199 pKa = 4.84 GYY201 pKa = 10.42 CGQEE205 pKa = 4.64 VVCLDD210 pKa = 4.44 DD211 pKa = 3.98 FYY213 pKa = 11.88 GWVTFDD219 pKa = 3.11 EE220 pKa = 4.66 LLRR223 pKa = 11.84 VLDD226 pKa = 4.89 RR227 pKa = 11.84 YY228 pKa = 9.48 PLRR231 pKa = 11.84 VPVKK235 pKa = 8.69 GAYY238 pKa = 9.4 VDD240 pKa = 4.35 FVSRR244 pKa = 11.84 EE245 pKa = 3.86 VYY247 pKa = 8.66 ITSNSTPDD255 pKa = 2.9 QWYY258 pKa = 7.94 TSEE261 pKa = 4.59 NIHH264 pKa = 6.32 GRR266 pKa = 11.84 LEE268 pKa = 3.82 ALYY271 pKa = 10.52 RR272 pKa = 11.84 RR273 pKa = 11.84 IDD275 pKa = 4.26 HH276 pKa = 6.29 YY277 pKa = 10.33 LTWDD281 pKa = 3.66 DD282 pKa = 4.25 RR283 pKa = 11.84 SKK285 pKa = 10.97 QLSPGNPMFPIAYY298 pKa = 9.59
Molecular weight: 34.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.571
IPC2_protein 7.834
IPC_protein 7.688
Toseland 7.249
ProMoST 8.331
Dawson 8.39
Bjellqvist 8.726
Wikipedia 8.317
Rodwell 8.404
Grimsley 7.22
Solomon 8.463
Lehninger 8.478
Nozaki 8.873
DTASelect 8.463
Thurlkill 8.507
EMBOSS 8.58
Sillero 8.814
Patrickios 4.113
IPC_peptide 8.463
IPC2_peptide 7.717
IPC2.peptide.svr19 7.932
Protein with the highest isoelectric point:
>tr|A0A3B8GVJ6|A0A3B8GVJ6_9CIRC ATP-dependent helicase Rep OS=Paguma larvata circovirus OX=2294094 PE=3 SV=1
MM1 pKa = 6.61 YY2 pKa = 9.93 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 FRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 5.17 HH14 pKa = 6.67 HH15 pKa = 5.24 PHH17 pKa = 5.01 HH18 pKa = 6.39 HH19 pKa = 5.98 RR20 pKa = 11.84 RR21 pKa = 11.84 YY22 pKa = 8.92 HH23 pKa = 6.69 IMRR26 pKa = 11.84 HH27 pKa = 4.45 HH28 pKa = 6.07 HH29 pKa = 6.5 FKK31 pKa = 10.76 RR32 pKa = 11.84 QFFHH36 pKa = 7.24 FLFRR40 pKa = 11.84 KK41 pKa = 6.55 QTSFFIQQVPAGYY54 pKa = 9.93 SKK56 pKa = 11.13 EE57 pKa = 4.12 GVKK60 pKa = 10.08 QGQFKK65 pKa = 9.61 PQYY68 pKa = 8.07 SWVRR72 pKa = 11.84 KK73 pKa = 9.56 HH74 pKa = 6.81 FFWTLNEE81 pKa = 4.05 FLRR84 pKa = 11.84 DD85 pKa = 3.85 SQSLGLFQYY94 pKa = 10.78 YY95 pKa = 10.3 RR96 pKa = 11.84 ILKK99 pKa = 10.32 AGITFKK105 pKa = 10.73 PQDD108 pKa = 4.72 PITDD112 pKa = 3.14 RR113 pKa = 11.84 RR114 pKa = 11.84 GYY116 pKa = 10.83 GFTAIDD122 pKa = 3.73 FTSKK126 pKa = 10.61 PEE128 pKa = 4.17 SGSFDD133 pKa = 4.26 PFWFWQTQNDD143 pKa = 5.1 PIPININSKK152 pKa = 4.36 TTKK155 pKa = 10.25 FFTTTKK161 pKa = 8.25 RR162 pKa = 11.84 HH163 pKa = 5.26 HH164 pKa = 6.96 RR165 pKa = 11.84 NVIPKK170 pKa = 10.02 PMLTDD175 pKa = 3.56 EE176 pKa = 4.65 PHH178 pKa = 7.07 PDD180 pKa = 3.12 LHH182 pKa = 7.34 FKK184 pKa = 9.48 EE185 pKa = 4.54 NWWFLNQKK193 pKa = 9.69 AGPWLTTADD202 pKa = 3.37 WLVPYY207 pKa = 10.68 LGLWWFLDD215 pKa = 3.81 LNFMNIVNPPTEE227 pKa = 4.33 DD228 pKa = 3.68 AGDD231 pKa = 3.34 KK232 pKa = 9.76 TLYY235 pKa = 10.25 IKK237 pKa = 10.01 TPMGLNFNFTKK248 pKa = 10.6 FIHH251 pKa = 5.72 VVFRR255 pKa = 11.84 DD256 pKa = 3.52 EE257 pKa = 4.16 CC258 pKa = 5.64
Molecular weight: 31.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.677
IPC_protein 10.072
Toseland 10.496
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.023
Grimsley 10.672
Solomon 10.672
Lehninger 10.643
Nozaki 10.452
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.526
Patrickios 10.716
IPC_peptide 10.672
IPC2_peptide 8.946
IPC2.peptide.svr19 8.743
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
556
258
298
278.0
33.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.417 ± 0.736
1.799 ± 0.951
5.216 ± 0.119
5.036 ± 1.566
7.014 ± 2.849
7.374 ± 1.836
3.777 ± 1.896
3.777 ± 0.851
6.655 ± 0.217
7.734 ± 0.772
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.799 ± 0.094
4.137 ± 0.347
5.755 ± 0.823
4.676 ± 0.244
8.273 ± 0.171
3.417 ± 0.048
6.475 ± 0.6
5.576 ± 1.668
3.237 ± 0.431
4.856 ± 0.922
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here