Amino acid dipepetide frequency for Spiroplasma diminutum CUAS-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.887AlaAla: 2.887 ± 0.129
0.398AlaCys: 0.398 ± 0.034
2.16AlaAsp: 2.16 ± 0.109
2.637AlaGlu: 2.637 ± 0.105
2.328AlaPhe: 2.328 ± 0.088
2.897AlaGly: 2.897 ± 0.112
0.621AlaHis: 0.621 ± 0.045
5.106AlaIle: 5.106 ± 0.128
4.759AlaLys: 4.759 ± 0.144
4.687AlaLeu: 4.687 ± 0.165
1.217AlaMet: 1.217 ± 0.061
3.206AlaAsn: 3.206 ± 0.103
1.142AlaPro: 1.142 ± 0.076
1.598AlaGln: 1.598 ± 0.078
1.66AlaArg: 1.66 ± 0.085
2.939AlaSer: 2.939 ± 0.092
2.832AlaThr: 2.832 ± 0.102
2.716AlaVal: 2.716 ± 0.113
0.446AlaTrp: 0.446 ± 0.042
1.759AlaTyr: 1.759 ± 0.08
0.0AlaXaa: 0.0 ± 0.0
Cys
0.312CysAla: 0.312 ± 0.03
0.096CysCys: 0.096 ± 0.021
0.418CysAsp: 0.418 ± 0.044
0.473CysGlu: 0.473 ± 0.044
0.415CysPhe: 0.415 ± 0.039
0.463CysGly: 0.463 ± 0.037
0.144CysHis: 0.144 ± 0.023
0.586CysIle: 0.586 ± 0.054
0.576CysLys: 0.576 ± 0.045
0.689CysLeu: 0.689 ± 0.052
0.141CysMet: 0.141 ± 0.026
0.353CysAsn: 0.353 ± 0.035
0.192CysPro: 0.192 ± 0.025
0.216CysGln: 0.216 ± 0.029
0.127CysArg: 0.127 ± 0.021
0.487CysSer: 0.487 ± 0.044
0.25CysThr: 0.25 ± 0.03
0.391CysVal: 0.391 ± 0.036
0.082CysTrp: 0.082 ± 0.016
0.261CysTyr: 0.261 ± 0.031
0.0CysXaa: 0.0 ± 0.0
Asp
2.088AspAla: 2.088 ± 0.097
0.377AspCys: 0.377 ± 0.036
2.644AspAsp: 2.644 ± 0.109
4.248AspGlu: 4.248 ± 0.127
3.703AspPhe: 3.703 ± 0.133
2.678AspGly: 2.678 ± 0.1
0.651AspHis: 0.651 ± 0.053
5.747AspIle: 5.747 ± 0.162
5.078AspLys: 5.078 ± 0.134
5.953AspLeu: 5.953 ± 0.142
1.08AspMet: 1.08 ± 0.05
3.487AspAsn: 3.487 ± 0.127
1.581AspPro: 1.581 ± 0.073
1.982AspGln: 1.982 ± 0.08
1.368AspArg: 1.368 ± 0.076
3.415AspSer: 3.415 ± 0.106
1.999AspThr: 1.999 ± 0.074
2.791AspVal: 2.791 ± 0.107
0.573AspTrp: 0.573 ± 0.043
2.568AspTyr: 2.568 ± 0.094
0.0AspXaa: 0.0 ± 0.0
Glu
3.185GluAla: 3.185 ± 0.148
0.411GluCys: 0.411 ± 0.036
3.775GluAsp: 3.775 ± 0.129
6.213GluGlu: 6.213 ± 0.199
3.929GluPhe: 3.929 ± 0.149
2.925GluGly: 2.925 ± 0.111
0.782GluHis: 0.782 ± 0.053
8.85GluIle: 8.85 ± 0.209
8.222GluLys: 8.222 ± 0.209
7.228GluLeu: 7.228 ± 0.157
1.858GluMet: 1.858 ± 0.076
6.583GluAsn: 6.583 ± 0.189
1.471GluPro: 1.471 ± 0.075
2.678GluGln: 2.678 ± 0.113
2.085GluArg: 2.085 ± 0.09
3.833GluSer: 3.833 ± 0.125
3.316GluThr: 3.316 ± 0.094
4.427GluVal: 4.427 ± 0.137
0.665GluTrp: 0.665 ± 0.049
2.86GluTyr: 2.86 ± 0.103
0.0GluXaa: 0.0 ± 0.0
Phe
2.757PheAla: 2.757 ± 0.089
0.422PheCys: 0.422 ± 0.04
3.439PheAsp: 3.439 ± 0.117
4.255PheGlu: 4.255 ± 0.14
2.651PhePhe: 2.651 ± 0.095
2.901PheGly: 2.901 ± 0.11
0.504PheHis: 0.504 ± 0.039
5.616PheIle: 5.616 ± 0.179
5.256PheLys: 5.256 ± 0.14
5.095PheLeu: 5.095 ± 0.135
1.173PheMet: 1.173 ± 0.064
4.012PheAsn: 4.012 ± 0.136
0.998PhePro: 0.998 ± 0.056
1.255PheGln: 1.255 ± 0.064
1.468PheArg: 1.468 ± 0.064
4.218PheSer: 4.218 ± 0.14
2.75PheThr: 2.75 ± 0.118
3.072PheVal: 3.072 ± 0.119
0.535PheTrp: 0.535 ± 0.05
2.092PheTyr: 2.092 ± 0.084
0.0PheXaa: 0.0 ± 0.0
Gly
2.952GlyAla: 2.952 ± 0.134
0.384GlyCys: 0.384 ± 0.036
2.428GlyAsp: 2.428 ± 0.105
3.227GlyGlu: 3.227 ± 0.124
3.134GlyPhe: 3.134 ± 0.101
3.12GlyGly: 3.12 ± 0.151
0.778GlyHis: 0.778 ± 0.061
5.215GlyIle: 5.215 ± 0.159
4.509GlyLys: 4.509 ± 0.13
4.656GlyLeu: 4.656 ± 0.151
1.221GlyMet: 1.221 ± 0.075
2.812GlyAsn: 2.812 ± 0.101
1.135GlyPro: 1.135 ± 0.058
1.564GlyGln: 1.564 ± 0.072
1.588GlyArg: 1.588 ± 0.086
3.264GlySer: 3.264 ± 0.113
2.88GlyThr: 2.88 ± 0.098
3.388GlyVal: 3.388 ± 0.133
0.607GlyTrp: 0.607 ± 0.056
2.122GlyTyr: 2.122 ± 0.096
0.0GlyXaa: 0.0 ± 0.0
His
0.579HisAla: 0.579 ± 0.045
0.117HisCys: 0.117 ± 0.017
0.655HisAsp: 0.655 ± 0.058
0.706HisGlu: 0.706 ± 0.052
0.734HisPhe: 0.734 ± 0.05
0.71HisGly: 0.71 ± 0.058
0.182HisHis: 0.182 ± 0.024
1.152HisIle: 1.152 ± 0.069
0.912HisLys: 0.912 ± 0.058
1.183HisLeu: 1.183 ± 0.064
0.24HisMet: 0.24 ± 0.032
0.792HisAsn: 0.792 ± 0.055
0.453HisPro: 0.453 ± 0.037
0.36HisGln: 0.36 ± 0.032
0.381HisArg: 0.381 ± 0.041
0.751HisSer: 0.751 ± 0.057
0.487HisThr: 0.487 ± 0.043
0.583HisVal: 0.583 ± 0.041
0.137HisTrp: 0.137 ± 0.021
0.501HisTyr: 0.501 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
5.531IleAla: 5.531 ± 0.13
0.922IleCys: 0.922 ± 0.061
6.265IleAsp: 6.265 ± 0.168
7.77IleGlu: 7.77 ± 0.181
5.675IlePhe: 5.675 ± 0.198
5.106IleGly: 5.106 ± 0.171
1.114IleHis: 1.114 ± 0.058
10.184IleIle: 10.184 ± 0.217
9.827IleLys: 9.827 ± 0.271
9.224IleLeu: 9.224 ± 0.194
2.04IleMet: 2.04 ± 0.094
8.164IleAsn: 8.164 ± 0.192
2.661IlePro: 2.661 ± 0.1
2.633IleGln: 2.633 ± 0.099
2.846IleArg: 2.846 ± 0.106
7.859IleSer: 7.859 ± 0.196
5.28IleThr: 5.28 ± 0.161
5.634IleVal: 5.634 ± 0.155
0.85IleTrp: 0.85 ± 0.057
3.899IleTyr: 3.899 ± 0.125
0.0IleXaa: 0.0 ± 0.0
Lys
4.176LysAla: 4.176 ± 0.138
0.405LysCys: 0.405 ± 0.039
5.85LysAsp: 5.85 ± 0.134
9.282LysGlu: 9.282 ± 0.208
4.41LysPhe: 4.41 ± 0.135
3.916LysGly: 3.916 ± 0.132
1.125LysHis: 1.125 ± 0.059
10.023LysIle: 10.023 ± 0.228
9.522LysLys: 9.522 ± 0.239
8.13LysLeu: 8.13 ± 0.169
2.678LysMet: 2.678 ± 0.091
8.668LysAsn: 8.668 ± 0.217
2.284LysPro: 2.284 ± 0.098
3.199LysGln: 3.199 ± 0.117
3.1LysArg: 3.1 ± 0.124
5.658LysSer: 5.658 ± 0.156
5.315LysThr: 5.315 ± 0.143
5.599LysVal: 5.599 ± 0.138
0.861LysTrp: 0.861 ± 0.057
4.324LysTyr: 4.324 ± 0.124
0.0LysXaa: 0.0 ± 0.0
Leu
4.626LeuAla: 4.626 ± 0.136
0.494LeuCys: 0.494 ± 0.037
5.359LeuAsp: 5.359 ± 0.148
7.228LeuGlu: 7.228 ± 0.179
5.208LeuPhe: 5.208 ± 0.179
4.495LeuGly: 4.495 ± 0.132
0.998LeuHis: 0.998 ± 0.057
9.323LeuIle: 9.323 ± 0.23
9.591LeuLys: 9.591 ± 0.201
8.147LeuLeu: 8.147 ± 0.166
1.934LeuMet: 1.934 ± 0.081
7.609LeuAsn: 7.609 ± 0.194
2.28LeuPro: 2.28 ± 0.096
2.441LeuGln: 2.441 ± 0.088
2.753LeuArg: 2.753 ± 0.111
6.402LeuSer: 6.402 ± 0.149
5.102LeuThr: 5.102 ± 0.133
5.058LeuVal: 5.058 ± 0.138
0.868LeuTrp: 0.868 ± 0.059
2.829LeuTyr: 2.829 ± 0.108
0.0LeuXaa: 0.0 ± 0.0
Met
1.334MetAla: 1.334 ± 0.07
0.154MetCys: 0.154 ± 0.025
1.077MetAsp: 1.077 ± 0.058
1.372MetGlu: 1.372 ± 0.069
1.176MetPhe: 1.176 ± 0.06
1.437MetGly: 1.437 ± 0.075
0.267MetHis: 0.267 ± 0.031
2.146MetIle: 2.146 ± 0.092
2.335MetLys: 2.335 ± 0.083
1.978MetLeu: 1.978 ± 0.077
0.473MetMet: 0.473 ± 0.04
1.735MetAsn: 1.735 ± 0.081
0.734MetPro: 0.734 ± 0.053
0.741MetGln: 0.741 ± 0.049
0.549MetArg: 0.549 ± 0.045
1.43MetSer: 1.43 ± 0.066
1.18MetThr: 1.18 ± 0.061
1.159MetVal: 1.159 ± 0.067
0.144MetTrp: 0.144 ± 0.021
0.792MetTyr: 0.792 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
2.877AsnAla: 2.877 ± 0.103
0.449AsnCys: 0.449 ± 0.042
4.307AsnAsp: 4.307 ± 0.126
5.908AsnGlu: 5.908 ± 0.166
4.368AsnPhe: 4.368 ± 0.152
3.631AsnGly: 3.631 ± 0.115
0.871AsnHis: 0.871 ± 0.057
7.749AsnIle: 7.749 ± 0.183
8.168AsnLys: 8.168 ± 0.199
7.197AsnLeu: 7.197 ± 0.17
1.416AsnMet: 1.416 ± 0.073
6.474AsnAsn: 6.474 ± 0.241
2.164AsnPro: 2.164 ± 0.099
2.801AsnGln: 2.801 ± 0.121
1.91AsnArg: 1.91 ± 0.094
5.38AsnSer: 5.38 ± 0.169
3.048AsnThr: 3.048 ± 0.116
3.566AsnVal: 3.566 ± 0.118
0.902AsnTrp: 0.902 ± 0.068
3.6AsnTyr: 3.6 ± 0.127
0.0AsnXaa: 0.0 ± 0.0
Pro
1.039ProAla: 1.039 ± 0.069
0.134ProCys: 0.134 ± 0.02
1.121ProAsp: 1.121 ± 0.067
2.126ProGlu: 2.126 ± 0.086
1.44ProPhe: 1.44 ± 0.072
1.485ProGly: 1.485 ± 0.073
0.305ProHis: 0.305 ± 0.033
2.668ProIle: 2.668 ± 0.107
2.04ProLys: 2.04 ± 0.098
2.17ProLeu: 2.17 ± 0.093
0.521ProMet: 0.521 ± 0.05
1.66ProAsn: 1.66 ± 0.066
0.473ProPro: 0.473 ± 0.046
0.84ProGln: 0.84 ± 0.059
0.607ProArg: 0.607 ± 0.046
1.581ProSer: 1.581 ± 0.081
1.45ProThr: 1.45 ± 0.072
1.581ProVal: 1.581 ± 0.082
0.274ProTrp: 0.274 ± 0.034
0.96ProTyr: 0.96 ± 0.066
0.0ProXaa: 0.0 ± 0.0
Gln
1.44GlnAla: 1.44 ± 0.08
0.113GlnCys: 0.113 ± 0.023
1.543GlnAsp: 1.543 ± 0.064
2.719GlnGlu: 2.719 ± 0.114
1.378GlnPhe: 1.378 ± 0.065
1.354GlnGly: 1.354 ± 0.07
0.329GlnHis: 0.329 ± 0.037
3.477GlnIle: 3.477 ± 0.113
3.065GlnLys: 3.065 ± 0.11
2.771GlnLeu: 2.771 ± 0.084
0.717GlnMet: 0.717 ± 0.048
2.5GlnAsn: 2.5 ± 0.107
0.651GlnPro: 0.651 ± 0.039
1.108GlnGln: 1.108 ± 0.094
1.094GlnArg: 1.094 ± 0.064
1.697GlnSer: 1.697 ± 0.081
1.478GlnThr: 1.478 ± 0.072
1.711GlnVal: 1.711 ± 0.08
0.346GlnTrp: 0.346 ± 0.039
1.121GlnTyr: 1.121 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
1.447ArgAla: 1.447 ± 0.074
0.195ArgCys: 0.195 ± 0.028
1.646ArgAsp: 1.646 ± 0.074
2.318ArgGlu: 2.318 ± 0.101
1.433ArgPhe: 1.433 ± 0.072
1.557ArgGly: 1.557 ± 0.087
0.374ArgHis: 0.374 ± 0.042
2.781ArgIle: 2.781 ± 0.082
2.843ArgLys: 2.843 ± 0.117
2.52ArgLeu: 2.52 ± 0.089
0.809ArgMet: 0.809 ± 0.057
2.208ArgAsn: 2.208 ± 0.096
0.785ArgPro: 0.785 ± 0.052
0.929ArgGln: 0.929 ± 0.051
1.125ArgArg: 1.125 ± 0.069
1.474ArgSer: 1.474 ± 0.064
1.478ArgThr: 1.478 ± 0.076
1.714ArgVal: 1.714 ± 0.075
0.237ArgTrp: 0.237 ± 0.033
1.183ArgTyr: 1.183 ± 0.066
0.0ArgXaa: 0.0 ± 0.0
Ser
2.956SerAla: 2.956 ± 0.105
0.466SerCys: 0.466 ± 0.037
3.1SerAsp: 3.1 ± 0.116
4.056SerGlu: 4.056 ± 0.138
3.583SerPhe: 3.583 ± 0.123
3.508SerGly: 3.508 ± 0.11
0.723SerHis: 0.723 ± 0.048
7.139SerIle: 7.139 ± 0.192
7.015SerLys: 7.015 ± 0.135
6.398SerLeu: 6.398 ± 0.178
1.358SerMet: 1.358 ± 0.071
5.147SerAsn: 5.147 ± 0.135
1.348SerPro: 1.348 ± 0.074
2.009SerGln: 2.009 ± 0.092
1.893SerArg: 1.893 ± 0.074
4.588SerSer: 4.588 ± 0.141
3.679SerThr: 3.679 ± 0.109
3.333SerVal: 3.333 ± 0.115
0.651SerTrp: 0.651 ± 0.053
2.558SerTyr: 2.558 ± 0.129
0.0SerXaa: 0.0 ± 0.0
Thr
2.462ThrAla: 2.462 ± 0.094
0.285ThrCys: 0.285 ± 0.032
2.472ThrAsp: 2.472 ± 0.098
2.942ThrGlu: 2.942 ± 0.095
2.969ThrPhe: 2.969 ± 0.102
3.244ThrGly: 3.244 ± 0.115
0.641ThrHis: 0.641 ± 0.048
5.277ThrIle: 5.277 ± 0.142
4.416ThrLys: 4.416 ± 0.122
4.831ThrLeu: 4.831 ± 0.131
1.063ThrMet: 1.063 ± 0.067
3.744ThrAsn: 3.744 ± 0.126
1.488ThrPro: 1.488 ± 0.076
1.526ThrGln: 1.526 ± 0.075
1.495ThrArg: 1.495 ± 0.073
3.367ThrSer: 3.367 ± 0.109
2.915ThrThr: 2.915 ± 0.123
3.083ThrVal: 3.083 ± 0.113
0.429ThrTrp: 0.429 ± 0.042
1.961ThrTyr: 1.961 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
3.155ValAla: 3.155 ± 0.118
0.49ValCys: 0.49 ± 0.037
3.072ValAsp: 3.072 ± 0.12
4.283ValGlu: 4.283 ± 0.15
2.853ValPhe: 2.853 ± 0.111
3.12ValGly: 3.12 ± 0.126
0.645ValHis: 0.645 ± 0.046
5.682ValIle: 5.682 ± 0.16
5.085ValLys: 5.085 ± 0.16
5.112ValLeu: 5.112 ± 0.13
1.238ValMet: 1.238 ± 0.068
3.758ValAsn: 3.758 ± 0.117
1.492ValPro: 1.492 ± 0.074
1.396ValGln: 1.396 ± 0.073
1.66ValArg: 1.66 ± 0.087
3.724ValSer: 3.724 ± 0.125
2.798ValThr: 2.798 ± 0.112
3.439ValVal: 3.439 ± 0.118
0.511ValTrp: 0.511 ± 0.039
2.15ValTyr: 2.15 ± 0.084
0.0ValXaa: 0.0 ± 0.0
Trp
0.459TrpAla: 0.459 ± 0.046
0.048TrpCys: 0.048 ± 0.014
0.494TrpAsp: 0.494 ± 0.044
0.549TrpGlu: 0.549 ± 0.046
0.641TrpPhe: 0.641 ± 0.049
0.422TrpGly: 0.422 ± 0.038
0.089TrpHis: 0.089 ± 0.017
1.08TrpIle: 1.08 ± 0.067
0.967TrpLys: 0.967 ± 0.062
0.936TrpLeu: 0.936 ± 0.065
0.329TrpMet: 0.329 ± 0.032
0.775TrpAsn: 0.775 ± 0.061
0.161TrpPro: 0.161 ± 0.024
0.24TrpGln: 0.24 ± 0.027
0.261TrpArg: 0.261 ± 0.029
0.6TrpSer: 0.6 ± 0.046
0.552TrpThr: 0.552 ± 0.046
0.569TrpVal: 0.569 ± 0.045
0.178TrpTrp: 0.178 ± 0.025
0.387TrpTyr: 0.387 ± 0.039
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.732TyrAla: 1.732 ± 0.073
0.312TyrCys: 0.312 ± 0.037
2.188TyrAsp: 2.188 ± 0.098
2.987TyrGlu: 2.987 ± 0.1
2.438TyrPhe: 2.438 ± 0.087
2.02TyrGly: 2.02 ± 0.076
0.47TyrHis: 0.47 ± 0.044
3.683TyrIle: 3.683 ± 0.12
4.334TyrLys: 4.334 ± 0.14
3.837TyrLeu: 3.837 ± 0.121
0.703TyrMet: 0.703 ± 0.045
3.1TyrAsn: 3.1 ± 0.105
0.984TyrPro: 0.984 ± 0.055
1.097TyrGln: 1.097 ± 0.065
1.09TyrArg: 1.09 ± 0.051
2.843TyrSer: 2.843 ± 0.125
1.821TyrThr: 1.821 ± 0.078
1.848TyrVal: 1.848 ± 0.076
0.442TyrTrp: 0.442 ± 0.042
1.622TyrTyr: 1.622 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 858 proteins (291642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski