Torque teno sus virus 1b
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0Y3X8|E0Y3X8_9VIRU ORF2 OS=Torque teno sus virus 1b OX=687387 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.51 EE2 pKa = 4.92 EE3 pKa = 3.49 RR4 pKa = 11.84 WLTVAYY10 pKa = 8.84 CAHH13 pKa = 7.1 GLFCDD18 pKa = 3.9 CKK20 pKa = 10.75 DD21 pKa = 3.68 PKK23 pKa = 10.89 KK24 pKa = 10.17 HH25 pKa = 6.64 LEE27 pKa = 3.92 KK28 pKa = 10.88 CLTDD32 pKa = 5.18 AIADD36 pKa = 3.82 AEE38 pKa = 4.42 GDD40 pKa = 3.68 RR41 pKa = 11.84 QEE43 pKa = 5.44 DD44 pKa = 4.1 GGTGGGDD51 pKa = 2.92 ATFDD55 pKa = 3.25 IGIDD59 pKa = 3.51 ALLAAAAQRR68 pKa = 3.68
Molecular weight: 7.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.398
IPC2_protein 4.444
IPC_protein 4.329
Toseland 4.139
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.24
Rodwell 4.164
Grimsley 4.062
Solomon 4.304
Lehninger 4.266
Nozaki 4.431
DTASelect 4.66
Thurlkill 4.19
EMBOSS 4.253
Sillero 4.457
Patrickios 3.592
IPC_peptide 4.304
IPC2_peptide 4.431
IPC2.peptide.svr19 4.392
Protein with the highest isoelectric point:
>tr|E0Y3X8|E0Y3X8_9VIRU ORF2 OS=Torque teno sus virus 1b OX=687387 GN=ORF2 PE=4 SV=1
MM1 pKa = 8.01 PYY3 pKa = 9.82 RR4 pKa = 11.84 RR5 pKa = 11.84 YY6 pKa = 9.5 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 PTRR15 pKa = 11.84 RR16 pKa = 11.84 WRR18 pKa = 11.84 HH19 pKa = 4.47 RR20 pKa = 11.84 RR21 pKa = 11.84 WRR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 7.81 FRR27 pKa = 11.84 YY28 pKa = 9.49 RR29 pKa = 11.84 YY30 pKa = 9.15 RR31 pKa = 11.84 RR32 pKa = 11.84 APRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 TKK40 pKa = 8.79 VRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 10.11 APVIQWNPPSRR58 pKa = 11.84 RR59 pKa = 11.84 TCLIEE64 pKa = 4.59 GFWPLSYY71 pKa = 10.62 GHH73 pKa = 7.13 WFRR76 pKa = 11.84 TCLPFRR82 pKa = 11.84 RR83 pKa = 11.84 KK84 pKa = 10.05 NGLIFTGGGCDD95 pKa = 2.95 WTQWSLQNLYY105 pKa = 10.4 HH106 pKa = 7.36 EE107 pKa = 4.67 KK108 pKa = 10.89 LNWRR112 pKa = 11.84 NIWTASNVGMEE123 pKa = 4.38 FTRR126 pKa = 11.84 FLRR129 pKa = 11.84 GKK131 pKa = 9.95 FYY133 pKa = 10.73 FFRR136 pKa = 11.84 HH137 pKa = 5.36 PWRR140 pKa = 11.84 NYY142 pKa = 8.91 IVTWDD147 pKa = 3.48 QDD149 pKa = 3.94 IPCKK153 pKa = 9.77 PLPYY157 pKa = 10.09 QNLHH161 pKa = 6.55 PLLMLLKK168 pKa = 9.36 KK169 pKa = 8.04 QHH171 pKa = 6.65 KK172 pKa = 9.91 LVLSQQNCNPNRR184 pKa = 11.84 KK185 pKa = 8.66 QKK187 pKa = 10.48 PVTLKK192 pKa = 10.25 FRR194 pKa = 11.84 PPPKK198 pKa = 9.71 LTSQWRR204 pKa = 11.84 LSRR207 pKa = 11.84 EE208 pKa = 3.95 LAKK211 pKa = 10.31 MPLIRR216 pKa = 11.84 LGVSFIDD223 pKa = 4.12 LTEE226 pKa = 4.07 PWLEE230 pKa = 3.71 GWGNAFYY237 pKa = 11.12 SVLGYY242 pKa = 9.89 EE243 pKa = 4.55 AIKK246 pKa = 10.32 EE247 pKa = 3.95 QGHH250 pKa = 5.32 WSNWAQVKK258 pKa = 8.7 YY259 pKa = 10.38 YY260 pKa = 9.75 WIYY263 pKa = 9.93 DD264 pKa = 3.69 TGVGNAVYY272 pKa = 10.73 VVMLKK277 pKa = 10.14 QEE279 pKa = 3.97 VDD281 pKa = 3.76 DD282 pKa = 5.19 NPGKK286 pKa = 9.45 MASTFKK292 pKa = 8.49 TTQGQHH298 pKa = 6.25 PNAIDD303 pKa = 4.13 HH304 pKa = 6.87 IEE306 pKa = 4.66 LINEE310 pKa = 4.42 GWPYY314 pKa = 10.19 WLYY317 pKa = 11.05 FFGKK321 pKa = 9.84 SEE323 pKa = 3.97 QDD325 pKa = 3.06 IKK327 pKa = 11.32 KK328 pKa = 9.07 EE329 pKa = 3.82 AHH331 pKa = 5.24 SQEE334 pKa = 3.81 IARR337 pKa = 11.84 EE338 pKa = 3.91 YY339 pKa = 9.47 ATNPKK344 pKa = 9.84 SKK346 pKa = 9.86 KK347 pKa = 9.88 LKK349 pKa = 9.95 IGIVGWASSNFTTPGSSQNTGGNTAAIQGGYY380 pKa = 7.85 VAWAGGQGKK389 pKa = 9.92 LNLGAGSIGNLYY401 pKa = 8.43 QQGWPSNQNWPNTNRR416 pKa = 11.84 DD417 pKa = 3.24 EE418 pKa = 4.83 TNFDD422 pKa = 2.67 WGLRR426 pKa = 11.84 SLCILRR432 pKa = 11.84 DD433 pKa = 3.61 NMQLGSQEE441 pKa = 5.12 LDD443 pKa = 3.89 DD444 pKa = 4.58 EE445 pKa = 4.72 CTMLSLFGPFVEE457 pKa = 4.54 KK458 pKa = 10.79 ANPIFATTDD467 pKa = 3.16 PKK469 pKa = 11.11 YY470 pKa = 10.66 FKK472 pKa = 10.64 PEE474 pKa = 3.89 LKK476 pKa = 10.28 DD477 pKa = 3.23 YY478 pKa = 10.97 NLIMKK483 pKa = 8.98 YY484 pKa = 10.38 AFKK487 pKa = 10.3 FQWGGHH493 pKa = 3.76 GTEE496 pKa = 4.41 RR497 pKa = 11.84 FKK499 pKa = 9.27 TTIGDD504 pKa = 3.85 PSTIPCPFEE513 pKa = 5.34 PGDD516 pKa = 3.9 RR517 pKa = 11.84 FHH519 pKa = 9.18 SGIQDD524 pKa = 3.36 PSKK527 pKa = 10.94 VQNTVLNPWDD537 pKa = 4.05 YY538 pKa = 11.7 DD539 pKa = 3.39 CDD541 pKa = 4.87 GIVRR545 pKa = 11.84 KK546 pKa = 8.78 DD547 pKa = 3.28 TLKK550 pKa = 10.91 RR551 pKa = 11.84 LLEE554 pKa = 4.48 LPTEE558 pKa = 4.31 TEE560 pKa = 4.42 EE561 pKa = 4.42 EE562 pKa = 4.15 EE563 pKa = 4.2 KK564 pKa = 10.55 AYY566 pKa = 9.87 PLLGQKK572 pKa = 7.42 TEE574 pKa = 4.39 KK575 pKa = 10.65 EE576 pKa = 4.16 PLSDD580 pKa = 3.53 SDD582 pKa = 3.95 EE583 pKa = 4.33 EE584 pKa = 4.55 SVISSTSSGSSQEE597 pKa = 4.44 EE598 pKa = 3.84 EE599 pKa = 4.09 TQRR602 pKa = 11.84 RR603 pKa = 11.84 KK604 pKa = 8.99 HH605 pKa = 5.93 HH606 pKa = 6.35 KK607 pKa = 7.96 PSKK610 pKa = 9.9 RR611 pKa = 11.84 RR612 pKa = 11.84 LLKK615 pKa = 10.16 HH616 pKa = 5.57 LQRR619 pKa = 11.84 VVKK622 pKa = 10.41 RR623 pKa = 11.84 MKK625 pKa = 9.97 TLL627 pKa = 3.31
Molecular weight: 73.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.2
IPC2_protein 9.37
IPC_protein 9.428
Toseland 10.058
ProMoST 9.75
Dawson 10.262
Bjellqvist 9.94
Wikipedia 10.423
Rodwell 10.643
Grimsley 10.321
Solomon 10.277
Lehninger 10.248
Nozaki 10.072
DTASelect 9.926
Thurlkill 10.116
EMBOSS 10.467
Sillero 10.175
Patrickios 10.116
IPC_peptide 10.277
IPC2_peptide 8.653
IPC2.peptide.svr19 8.343
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
695
68
627
347.5
40.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.892 ± 4.87
1.871 ± 1.731
4.892 ± 3.601
5.755 ± 0.689
4.173 ± 0.531
7.626 ± 1.786
2.446 ± 0.214
4.317 ± 0.041
7.194 ± 0.566
8.489 ± 0.144
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.583 ± 0.048
4.748 ± 2.049
6.043 ± 1.973
4.892 ± 0.842
8.633 ± 1.822
5.18 ± 2.236
6.043 ± 0.069
3.453 ± 0.856
3.885 ± 1.042
3.885 ± 1.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here