Vibrio phage pYD38-B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9TRL2|R9TRL2_9CAUD Uncharacterized protein OS=Vibrio phage pYD38-B OX=929835 GN=VPSG_00038 PE=4 SV=1
MM1 pKa = 7.49TLQDD5 pKa = 3.57YY6 pKa = 9.55EE7 pKa = 4.29EE8 pKa = 5.07RR9 pKa = 11.84FLGEE13 pKa = 5.56LIEE16 pKa = 4.21NWPGIDD22 pKa = 2.85WDD24 pKa = 3.9KK25 pKa = 11.32YY26 pKa = 9.06VRR28 pKa = 11.84QCHH31 pKa = 5.28EE32 pKa = 4.57EE33 pKa = 4.08YY34 pKa = 9.87MDD36 pKa = 5.61SIDD39 pKa = 3.63EE40 pKa = 4.14AKK42 pKa = 10.04IAEE45 pKa = 4.38WEE47 pKa = 4.04NSLL50 pKa = 4.94

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TN40|R9TN40_9CAUD Uncharacterized protein OS=Vibrio phage pYD38-B OX=929835 GN=VPSG_00044 PE=4 SV=1
MM1 pKa = 7.67PSANEE6 pKa = 3.25IMAGFKK12 pKa = 10.28SRR14 pKa = 11.84HH15 pKa = 4.69SQPKK19 pKa = 9.96KK20 pKa = 7.37RR21 pKa = 11.84TNWRR25 pKa = 11.84KK26 pKa = 8.01EE27 pKa = 3.78LKK29 pKa = 10.44LEE31 pKa = 3.98RR32 pKa = 11.84EE33 pKa = 4.11RR34 pKa = 11.84VIDD37 pKa = 3.74EE38 pKa = 4.5AIKK41 pKa = 10.48RR42 pKa = 11.84IRR44 pKa = 11.84DD45 pKa = 3.32KK46 pKa = 11.28LRR48 pKa = 11.84AEE50 pKa = 4.17NVPEE54 pKa = 4.07SFKK57 pKa = 10.45RR58 pKa = 11.84GHH60 pKa = 5.84YY61 pKa = 9.91AAITEE66 pKa = 4.19LEE68 pKa = 4.19LMKK71 pKa = 10.8SEE73 pKa = 4.36VKK75 pKa = 10.6

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11041

49

1069

193.7

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.925 ± 0.698

1.386 ± 0.248

5.824 ± 0.227

7.662 ± 0.31

3.722 ± 0.238

6.965 ± 0.364

1.793 ± 0.237

6.05 ± 0.268

7.508 ± 0.54

7.771 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.279 ± 0.187

5.353 ± 0.225

3.079 ± 0.215

3.895 ± 0.293

4.248 ± 0.298

6.929 ± 0.369

5.416 ± 0.281

6.322 ± 0.252

1.431 ± 0.181

3.442 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski