Okra yellow vein disease associated sequence
Average proteome isoelectric point is 5.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7T471|Q7T471_9VIRU Putative C1 protein OS=Okra yellow vein disease associated sequence OX=169693 GN=C1 PE=4 SV=1
MM1 pKa = 7.77 TITLRR6 pKa = 11.84 NTKK9 pKa = 9.41 GVHH12 pKa = 4.93 FRR14 pKa = 11.84 VDD16 pKa = 3.16 VFLKK20 pKa = 10.47 DD21 pKa = 3.52 DD22 pKa = 5.19 HH23 pKa = 7.24 IDD25 pKa = 3.6 CKK27 pKa = 10.88 IEE29 pKa = 3.77 VTATKK34 pKa = 10.27 EE35 pKa = 3.83 SYY37 pKa = 9.89 LRR39 pKa = 11.84 KK40 pKa = 8.41 STFIIPYY47 pKa = 8.72 HH48 pKa = 5.87 FADD51 pKa = 3.56 VFIPFDD57 pKa = 4.02 FNGSEE62 pKa = 4.13 EE63 pKa = 4.54 EE64 pKa = 4.1 IMHH67 pKa = 6.97 GLRR70 pKa = 11.84 NMFKK74 pKa = 9.9 DD75 pKa = 3.47 TTHH78 pKa = 6.42 QMIRR82 pKa = 11.84 TEE84 pKa = 4.0 EE85 pKa = 4.03 LVDD88 pKa = 3.8 VVDD91 pKa = 4.39 MVMAEE96 pKa = 4.1 NVNVLGMEE104 pKa = 5.24 LINHH108 pKa = 5.25 YY109 pKa = 10.51 RR110 pKa = 11.84 IINKK114 pKa = 9.97 SSVV117 pKa = 2.84
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.513
IPC2_protein 5.41
IPC_protein 5.385
Toseland 5.766
ProMoST 5.677
Dawson 5.575
Bjellqvist 5.614
Wikipedia 5.563
Rodwell 5.55
Grimsley 5.931
Solomon 5.575
Lehninger 5.563
Nozaki 5.804
DTASelect 5.995
Thurlkill 5.944
EMBOSS 5.906
Sillero 5.906
Patrickios 4.24
IPC_peptide 5.588
IPC2_peptide 5.919
IPC2.peptide.svr19 5.892
Protein with the highest isoelectric point:
>tr|Q7T471|Q7T471_9VIRU Putative C1 protein OS=Okra yellow vein disease associated sequence OX=169693 GN=C1 PE=4 SV=1
MM1 pKa = 7.77 TITLRR6 pKa = 11.84 NTKK9 pKa = 9.41 GVHH12 pKa = 4.93 FRR14 pKa = 11.84 VDD16 pKa = 3.16 VFLKK20 pKa = 10.47 DD21 pKa = 3.52 DD22 pKa = 5.19 HH23 pKa = 7.24 IDD25 pKa = 3.6 CKK27 pKa = 10.88 IEE29 pKa = 3.77 VTATKK34 pKa = 10.27 EE35 pKa = 3.83 SYY37 pKa = 9.89 LRR39 pKa = 11.84 KK40 pKa = 8.41 STFIIPYY47 pKa = 8.72 HH48 pKa = 5.87 FADD51 pKa = 3.56 VFIPFDD57 pKa = 4.02 FNGSEE62 pKa = 4.13 EE63 pKa = 4.54 EE64 pKa = 4.1 IMHH67 pKa = 6.97 GLRR70 pKa = 11.84 NMFKK74 pKa = 9.9 DD75 pKa = 3.47 TTHH78 pKa = 6.42 QMIRR82 pKa = 11.84 TEE84 pKa = 4.0 EE85 pKa = 4.03 LVDD88 pKa = 3.8 VVDD91 pKa = 4.39 MVMAEE96 pKa = 4.1 NVNVLGMEE104 pKa = 5.24 LINHH108 pKa = 5.25 YY109 pKa = 10.51 RR110 pKa = 11.84 IINKK114 pKa = 9.97 SSVV117 pKa = 2.84
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.513
IPC2_protein 5.41
IPC_protein 5.385
Toseland 5.766
ProMoST 5.677
Dawson 5.575
Bjellqvist 5.614
Wikipedia 5.563
Rodwell 5.55
Grimsley 5.931
Solomon 5.575
Lehninger 5.563
Nozaki 5.804
DTASelect 5.995
Thurlkill 5.944
EMBOSS 5.906
Sillero 5.906
Patrickios 4.24
IPC_peptide 5.588
IPC2_peptide 5.919
IPC2.peptide.svr19 5.892
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
117
117
117
117.0
13.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.564 ± 0.0
0.855 ± 0.0
7.692 ± 0.0
7.692 ± 0.0
6.838 ± 0.0
3.419 ± 0.0
5.128 ± 0.0
9.402 ± 0.0
5.983 ± 0.0
5.983 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.983 ± 0.0
5.983 ± 0.0
1.709 ± 0.0
0.855 ± 0.0
5.128 ± 0.0
4.274 ± 0.0
7.692 ± 0.0
10.256 ± 0.0
0.0 ± 0.0
2.564 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here