Biratnagar virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7EIZ3|A0A1U7EIZ3_9VIRU Non-structural protein 2 OS=Biratnagar virus OX=1955197 PE=4 SV=1
MM1 pKa = 7.18FLIVFLFSTAFAFDD15 pKa = 4.7RR16 pKa = 11.84YY17 pKa = 9.46PLPEE21 pKa = 3.99LVEE24 pKa = 4.18FHH26 pKa = 7.18SEE28 pKa = 3.8LDD30 pKa = 3.42RR31 pKa = 11.84LGMLDD36 pKa = 3.41IEE38 pKa = 5.51LIGKK42 pKa = 9.41LYY44 pKa = 10.61SLQPFSDD51 pKa = 3.85FKK53 pKa = 11.43VYY55 pKa = 9.7EE56 pKa = 4.01ANAWNKK62 pKa = 10.39LIGYY66 pKa = 7.34HH67 pKa = 6.19CPSGQTVYY75 pKa = 10.59PLEE78 pKa = 4.33HH79 pKa = 6.78SFLKK83 pKa = 10.61DD84 pKa = 3.79LYY86 pKa = 10.26TCIPKK91 pKa = 10.29PKK93 pKa = 9.95VLNSQYY99 pKa = 10.84LVYY102 pKa = 10.42RR103 pKa = 11.84IKK105 pKa = 10.65PSSHH109 pKa = 7.26DD110 pKa = 3.69NVTVQCKK117 pKa = 8.39NRR119 pKa = 11.84YY120 pKa = 8.24YY121 pKa = 9.77NVSVAFCQIEE131 pKa = 4.58SYY133 pKa = 10.97RR134 pKa = 11.84FLIDD138 pKa = 3.44NKK140 pKa = 10.04GDD142 pKa = 3.07TWFTSDD148 pKa = 3.24YY149 pKa = 9.85CLSNRR154 pKa = 11.84YY155 pKa = 7.22MTDD158 pKa = 2.96NDD160 pKa = 3.69GKK162 pKa = 10.58EE163 pKa = 4.2LFTVSHH169 pKa = 6.95TSTDD173 pKa = 3.44YY174 pKa = 11.29SVTFEE179 pKa = 4.63FPPPEE184 pKa = 4.49CPFYY188 pKa = 10.48PYY190 pKa = 10.73DD191 pKa = 3.46VYY193 pKa = 11.43YY194 pKa = 10.52EE195 pKa = 4.14KK196 pKa = 10.81SLPATVCIDD205 pKa = 3.45SSPEE209 pKa = 3.73FKK211 pKa = 10.81SCVTGAKK218 pKa = 10.17CEE220 pKa = 3.94VHH222 pKa = 6.6PPYY225 pKa = 10.69EE226 pKa = 4.11LLPVSTHH233 pKa = 6.15SEE235 pKa = 3.72LHH237 pKa = 5.93FKK239 pKa = 11.09VNGTCSVAFMTFTEE253 pKa = 4.55PTTITYY259 pKa = 9.59HH260 pKa = 5.99IKK262 pKa = 10.47SALSTSFIDD271 pKa = 4.33AVLHH275 pKa = 6.47ALLSLLRR282 pKa = 11.84PLIDD286 pKa = 4.22ALLEE290 pKa = 4.03LVTYY294 pKa = 10.43IIDD297 pKa = 3.65TLVTIFTSSEE307 pKa = 3.62FVAIYY312 pKa = 9.91QKK314 pKa = 10.18IYY316 pKa = 10.93HH317 pKa = 7.24LILSVLQNVFDD328 pKa = 4.46YY329 pKa = 10.67IIDD332 pKa = 3.76VAVPKK337 pKa = 10.73IMDD340 pKa = 4.58LFYY343 pKa = 11.16EE344 pKa = 4.51STLKK348 pKa = 10.13TKK350 pKa = 10.32FIILTFIYY358 pKa = 10.6VYY360 pKa = 10.46FKK362 pKa = 9.32YY363 pKa = 9.51TKK365 pKa = 10.22FIYY368 pKa = 10.24ALSVCVVVCLFFSKK382 pKa = 10.89

Molecular weight: 44.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7EIU8|A0A1U7EIU8_9VIRU Uncharacterized protein OS=Biratnagar virus OX=1955197 PE=4 SV=1
MM1 pKa = 6.82VLKK4 pKa = 9.91RR5 pKa = 11.84TGTVVRR11 pKa = 11.84PTTTSRR17 pKa = 11.84RR18 pKa = 11.84AVVAAPVKK26 pKa = 9.97RR27 pKa = 11.84AVNRR31 pKa = 11.84VKK33 pKa = 11.03NFGSNLDD40 pKa = 3.5FSKK43 pKa = 10.61YY44 pKa = 8.17FQYY47 pKa = 10.13ATSVLSNTTFLLIFAFSAFLCYY69 pKa = 10.64NYY71 pKa = 7.56TTKK74 pKa = 10.91GEE76 pKa = 3.99KK77 pKa = 9.37SHH79 pKa = 6.24IVKK82 pKa = 10.26FVGNFVTQFPSFKK95 pKa = 10.55DD96 pKa = 3.63SEE98 pKa = 4.3CSILGFVQFFVPFIPAVVSVRR119 pKa = 11.84PSNRR123 pKa = 11.84VATLVGVALYY133 pKa = 10.39YY134 pKa = 10.71VFVPEE139 pKa = 4.11RR140 pKa = 11.84TVYY143 pKa = 10.13EE144 pKa = 4.18YY145 pKa = 10.75LVHH148 pKa = 6.97GIIMYY153 pKa = 10.24LILRR157 pKa = 11.84TEE159 pKa = 3.8VRR161 pKa = 11.84AYY163 pKa = 10.78RR164 pKa = 11.84LIGFAMLFLSYY175 pKa = 10.55IMQFALPLPSGDD187 pKa = 3.28GHH189 pKa = 6.58NCTSSS194 pKa = 2.75

Molecular weight: 21.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2841

194

2265

947.0

109.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.857 ± 0.508

2.358 ± 0.284

6.617 ± 1.59

4.963 ± 0.657

6.16 ± 1.335

2.957 ± 0.586

2.851 ± 0.357

7.005 ± 0.674

6.688 ± 0.847

9.187 ± 0.561

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.31

4.717 ± 0.662

4.4 ± 0.38

2.64 ± 0.344

4.435 ± 0.836

7.673 ± 0.39

6.512 ± 0.553

7.779 ± 1.477

0.422 ± 0.111

5.561 ± 0.667
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski