Enterococcus phage vB_EfaP_IME199

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Sarlesvirinae; Minhovirus; Enterococcus virus IME199

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MYH8|A0A0S2MYH8_9CAUD DNA-directed DNA polymerase OS=Enterococcus phage vB_EfaP_IME199 OX=1747351 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.23NFKK5 pKa = 8.37ITYY8 pKa = 7.31YY9 pKa = 10.52DD10 pKa = 3.0AWEE13 pKa = 4.46GQTMEE18 pKa = 4.58TYY20 pKa = 10.51IFAQSALEE28 pKa = 3.96AEE30 pKa = 4.82VIFTQEE36 pKa = 3.74EE37 pKa = 4.36DD38 pKa = 3.87SFCQVIDD45 pKa = 3.68VQEE48 pKa = 4.36EE49 pKa = 4.07II50 pKa = 4.53

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MYH6|A0A0S2MYH6_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaP_IME199 OX=1747351 PE=4 SV=1
MM1 pKa = 6.48ITKK4 pKa = 10.14GIAGRR9 pKa = 11.84RR10 pKa = 11.84QGKK13 pKa = 8.92VKK15 pKa = 10.49GVVIHH20 pKa = 6.88NDD22 pKa = 3.06AGSNGATCSFYY33 pKa = 10.65RR34 pKa = 11.84GYY36 pKa = 9.44LTDD39 pKa = 4.5ANAAYY44 pKa = 9.86GFAHH48 pKa = 7.48WYY50 pKa = 8.24VASDD54 pKa = 4.71GILQVEE60 pKa = 4.99DD61 pKa = 3.98EE62 pKa = 5.46SNMAWHH68 pKa = 6.85TANSYY73 pKa = 11.23GNANFIGIEE82 pKa = 4.1ACQSMGNLAQFLEE95 pKa = 4.16NEE97 pKa = 3.67KK98 pKa = 10.65RR99 pKa = 11.84AIKK102 pKa = 10.09LAAEE106 pKa = 3.69ILKK109 pKa = 10.52RR110 pKa = 11.84HH111 pKa = 5.78GLTPNTTTVRR121 pKa = 11.84LHH123 pKa = 5.57NTFSSTACPHH133 pKa = 7.01RR134 pKa = 11.84SQEE137 pKa = 3.87QHH139 pKa = 5.55GAGSKK144 pKa = 7.22TQAYY148 pKa = 8.86FIAEE152 pKa = 4.37IKK154 pKa = 10.51KK155 pKa = 9.86HH156 pKa = 5.49MNGSTPAPSKK166 pKa = 9.53PTTPSQHH173 pKa = 6.68DD174 pKa = 3.84KK175 pKa = 11.13AVSASPAKK183 pKa = 10.3HH184 pKa = 6.06QGNAWGKK191 pKa = 7.85LDD193 pKa = 4.24KK194 pKa = 11.04YY195 pKa = 10.67RR196 pKa = 11.84EE197 pKa = 4.01EE198 pKa = 3.99PAGKK202 pKa = 9.59IRR204 pKa = 11.84VAGWLVPDD212 pKa = 4.95KK213 pKa = 10.62PQGPIGKK220 pKa = 8.29YY221 pKa = 9.59AYY223 pKa = 10.33VIFMQHH229 pKa = 5.07GTNKK233 pKa = 10.09EE234 pKa = 3.93LTRR237 pKa = 11.84VQSAGIKK244 pKa = 10.33RR245 pKa = 11.84PDD247 pKa = 3.14VKK249 pKa = 10.48KK250 pKa = 10.45AYY252 pKa = 9.82SYY254 pKa = 11.15QGGQEE259 pKa = 4.09LGFDD263 pKa = 3.76VTVKK267 pKa = 10.47KK268 pKa = 10.75AQFKK272 pKa = 9.54GKK274 pKa = 9.98KK275 pKa = 8.93VDD277 pKa = 3.93VILRR281 pKa = 11.84RR282 pKa = 11.84ANKK285 pKa = 10.27ANGEE289 pKa = 4.25VAVNDD294 pKa = 3.37VRR296 pKa = 11.84IDD298 pKa = 3.88SIYY301 pKa = 10.17LTLL304 pKa = 4.81

Molecular weight: 33.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

5950

45

779

270.5

30.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.218 ± 0.537

0.655 ± 0.171

5.916 ± 0.203

7.059 ± 0.588

4.639 ± 0.402

6.017 ± 0.573

1.832 ± 0.25

6.437 ± 0.254

7.412 ± 0.451

7.832 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.153

7.496 ± 0.37

3.496 ± 0.363

4.067 ± 0.334

3.445 ± 0.315

5.798 ± 0.314

7.244 ± 0.474

5.697 ± 0.309

1.176 ± 0.13

4.924 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski