Maize associated rhabdovirus
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X9Y2V8|A0A1X9Y2V8_9RHAB Putative P protein OS=Maize associated rhabdovirus OX=2003308 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.23 APKK5 pKa = 10.69 ASDD8 pKa = 3.32 IFSQVPDD15 pKa = 4.2 DD16 pKa = 4.21 VEE18 pKa = 4.94 PDD20 pKa = 3.57 YY21 pKa = 11.36 EE22 pKa = 4.23 IDD24 pKa = 3.45 EE25 pKa = 4.87 DD26 pKa = 4.97 EE27 pKa = 4.86 IFAKK31 pKa = 10.4 PGPTQAEE38 pKa = 4.29 PNNQEE43 pKa = 3.92 DD44 pKa = 4.01 ALDD47 pKa = 3.86 EE48 pKa = 4.38 EE49 pKa = 5.23 EE50 pKa = 5.01 EE51 pKa = 4.41 EE52 pKa = 4.36 QHH54 pKa = 6.61 SDD56 pKa = 3.49 PPPASGNPPDD66 pKa = 4.37 YY67 pKa = 10.62 RR68 pKa = 11.84 FKK70 pKa = 10.57 TISAMTDD77 pKa = 3.73 FVRR80 pKa = 11.84 KK81 pKa = 7.04 TANDD85 pKa = 3.11 KK86 pKa = 11.37 GITVKK91 pKa = 10.77 KK92 pKa = 9.21 EE93 pKa = 3.34 WMNILTRR100 pKa = 11.84 EE101 pKa = 4.03 WHH103 pKa = 6.43 KK104 pKa = 11.53 KK105 pKa = 8.67 NADD108 pKa = 3.33 VYY110 pKa = 10.96 ASHH113 pKa = 7.56 IDD115 pKa = 3.72 FFFLGITGEE124 pKa = 4.31 RR125 pKa = 11.84 NLSVEE130 pKa = 4.64 KK131 pKa = 10.66 DD132 pKa = 3.61 FKK134 pKa = 11.39 DD135 pKa = 2.87 IAKK138 pKa = 10.4 RR139 pKa = 11.84 MNDD142 pKa = 3.14 DD143 pKa = 3.43 VNRR146 pKa = 11.84 VSGVGKK152 pKa = 8.55 QLKK155 pKa = 10.13 DD156 pKa = 3.29 ISDD159 pKa = 3.87 KK160 pKa = 10.5 MSKK163 pKa = 10.73 DD164 pKa = 3.35 LDD166 pKa = 3.73 AKK168 pKa = 10.46 MKK170 pKa = 10.85 LLDD173 pKa = 4.07 AKK175 pKa = 10.71 LEE177 pKa = 4.26 KK178 pKa = 10.05 FNSMMEE184 pKa = 4.17 KK185 pKa = 10.37 NEE187 pKa = 4.02 SFLEE191 pKa = 4.32 SASKK195 pKa = 10.19 QSSVASWAAEE205 pKa = 3.79 ARR207 pKa = 11.84 DD208 pKa = 4.14 EE209 pKa = 4.31 PPKK212 pKa = 10.15 TKK214 pKa = 9.22 KK215 pKa = 8.87 TSPRR219 pKa = 11.84 MKK221 pKa = 9.84 ILRR224 pKa = 11.84 FLSGIGFSQVEE235 pKa = 4.25 AQNVIKK241 pKa = 10.82 VGAHH245 pKa = 6.11 HH246 pKa = 7.49 IITEE250 pKa = 4.03 EE251 pKa = 3.7 MMEE254 pKa = 4.15 NVFTGLADD262 pKa = 4.35 DD263 pKa = 4.31 EE264 pKa = 4.85 LKK266 pKa = 11.0 EE267 pKa = 4.29 EE268 pKa = 4.36 YY269 pKa = 10.4 EE270 pKa = 4.7 QMLCLEE276 pKa = 4.29 GQKK279 pKa = 10.48 LVAEE283 pKa = 4.34 ARR285 pKa = 11.84 EE286 pKa = 4.17 KK287 pKa = 10.96 KK288 pKa = 10.1 NKK290 pKa = 10.14 KK291 pKa = 9.36 KK292 pKa = 10.63 DD293 pKa = 3.2 IYY295 pKa = 11.09 DD296 pKa = 4.02 DD297 pKa = 3.92 YY298 pKa = 12.15
Molecular weight: 33.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.902
IPC2_protein 4.813
IPC_protein 4.749
Toseland 4.609
ProMoST 4.851
Dawson 4.698
Bjellqvist 4.851
Wikipedia 4.571
Rodwell 4.596
Grimsley 4.52
Solomon 4.698
Lehninger 4.66
Nozaki 4.813
DTASelect 4.965
Thurlkill 4.609
EMBOSS 4.584
Sillero 4.876
Patrickios 4.38
IPC_peptide 4.711
IPC2_peptide 4.863
IPC2.peptide.svr19 4.857
Protein with the highest isoelectric point:
>tr|A0A1X9Y2V3|A0A1X9Y2V3_9RHAB Putative G protein OS=Maize associated rhabdovirus OX=2003308 PE=4 SV=1
MM1 pKa = 7.97 RR2 pKa = 11.84 PRR4 pKa = 11.84 SLGFLHH10 pKa = 7.18 LRR12 pKa = 11.84 ILRR15 pKa = 11.84 VKK17 pKa = 8.65 MASGSEE23 pKa = 3.76 QHH25 pKa = 6.59 IDD27 pKa = 4.24 KK28 pKa = 10.91 DD29 pKa = 3.66 LTDD32 pKa = 3.99 AFSNVPEE39 pKa = 4.06 NLAVNTVPDD48 pKa = 3.74 EE49 pKa = 4.33 TFSDD53 pKa = 4.05 DD54 pKa = 3.2 AFKK57 pKa = 10.97 SHH59 pKa = 6.35 SVYY62 pKa = 10.51 EE63 pKa = 4.26 INEE66 pKa = 3.97 LTIKK70 pKa = 10.47 QVVHH74 pKa = 6.17 HH75 pKa = 7.08 AYY77 pKa = 10.02 KK78 pKa = 10.83 LFRR81 pKa = 11.84 NMGQKK86 pKa = 8.24 TCHH89 pKa = 6.5 AIIPYY94 pKa = 10.05 SILAMALRR102 pKa = 11.84 LPTPKK107 pKa = 10.17 KK108 pKa = 10.38 KK109 pKa = 9.47 STTSFLTSHH118 pKa = 6.58 KK119 pKa = 9.86 PSGGTALEE127 pKa = 3.71 QGIASKK133 pKa = 10.64 LYY135 pKa = 9.72 PSLDD139 pKa = 3.37 LSTEE143 pKa = 3.95 EE144 pKa = 4.06 AAKK147 pKa = 10.32 PDD149 pKa = 3.77 AEE151 pKa = 4.52 KK152 pKa = 10.93 KK153 pKa = 10.39 RR154 pKa = 11.84 LTATKK159 pKa = 10.41 VFGSEE164 pKa = 3.44 EE165 pKa = 4.02 DD166 pKa = 3.75 KK167 pKa = 11.01 EE168 pKa = 4.61 FKK170 pKa = 10.38 AVRR173 pKa = 11.84 EE174 pKa = 4.09 LEE176 pKa = 4.13 EE177 pKa = 4.54 EE178 pKa = 3.97 IVFQFGCFLSAFLLKK193 pKa = 10.35 LLCKK197 pKa = 10.37 NPEE200 pKa = 4.15 NIITGWDD207 pKa = 3.36 AMRR210 pKa = 11.84 GRR212 pKa = 11.84 YY213 pKa = 7.63 STFFSEE219 pKa = 4.64 SAPSEE224 pKa = 4.13 VKK226 pKa = 10.38 RR227 pKa = 11.84 PSRR230 pKa = 11.84 EE231 pKa = 3.43 WLTEE235 pKa = 3.74 LKK237 pKa = 10.78 NLFSSDD243 pKa = 3.23 PMISRR248 pKa = 11.84 TWVRR252 pKa = 11.84 TFSEE256 pKa = 4.9 AEE258 pKa = 3.77 DD259 pKa = 3.7 HH260 pKa = 7.0 LPAGEE265 pKa = 4.12 QQVGMLRR272 pKa = 11.84 YY273 pKa = 8.84 LALMPFSYY281 pKa = 10.03 TGLHH285 pKa = 6.43 GYY287 pKa = 10.42 KK288 pKa = 10.4 LFLDD292 pKa = 4.0 VSRR295 pKa = 11.84 ASNLSNKK302 pKa = 8.64 WLLTEE307 pKa = 4.11 MTSPMTSKK315 pKa = 10.76 ALKK318 pKa = 10.15 LIAHH322 pKa = 6.91 ILINFEE328 pKa = 4.02 SRR330 pKa = 11.84 IGEE333 pKa = 4.23 KK334 pKa = 10.19 KK335 pKa = 10.33 KK336 pKa = 10.93 SAFKK340 pKa = 9.39 YY341 pKa = 9.87 ARR343 pKa = 11.84 MMSTSYY349 pKa = 10.77 FQDD352 pKa = 3.44 LQTKK356 pKa = 8.21 NCAGLVYY363 pKa = 10.8 LEE365 pKa = 4.02 VKK367 pKa = 9.89 ILKK370 pKa = 10.09 KK371 pKa = 10.69 FEE373 pKa = 4.34 AFGTNSNPEE382 pKa = 4.1 NIIGIEE388 pKa = 4.03 KK389 pKa = 10.37 VPAEE393 pKa = 4.32 HH394 pKa = 7.58 KK395 pKa = 11.21 AMLSKK400 pKa = 10.73 AADD403 pKa = 4.54 FIVSAAPQRR412 pKa = 11.84 NLGRR416 pKa = 11.84 YY417 pKa = 8.31 SEE419 pKa = 4.41 SMRR422 pKa = 11.84 KK423 pKa = 9.9 AMIPEE428 pKa = 4.66 HH429 pKa = 6.79 KK430 pKa = 9.62 PKK432 pKa = 10.17 QHH434 pKa = 4.92 QQVAKK439 pKa = 10.5 KK440 pKa = 10.25 EE441 pKa = 4.43 DD442 pKa = 3.3 IFGG445 pKa = 3.8
Molecular weight: 50.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.658
IPC2_protein 8.507
IPC_protein 8.39
Toseland 9.268
ProMoST 8.975
Dawson 9.502
Bjellqvist 9.224
Wikipedia 9.633
Rodwell 9.882
Grimsley 9.545
Solomon 9.545
Lehninger 9.516
Nozaki 9.385
DTASelect 9.165
Thurlkill 9.37
EMBOSS 9.692
Sillero 9.472
Patrickios 5.169
IPC_peptide 9.545
IPC2_peptide 7.717
IPC2.peptide.svr19 7.757
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3631
167
2066
605.2
68.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.426 ± 0.682
1.708 ± 0.703
5.095 ± 0.805
6.995 ± 0.875
4.241 ± 0.427
5.288 ± 0.417
2.368 ± 0.128
8.014 ± 0.786
6.968 ± 1.083
9.226 ± 0.824
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.167 ± 0.306
4.351 ± 0.132
4.875 ± 0.054
2.809 ± 0.337
5.095 ± 0.618
8.207 ± 0.566
5.839 ± 0.409
5.095 ± 0.665
1.652 ± 0.374
3.58 ± 0.429
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here