Amino acid dipepetide frequency for Spiroplasma taiwanense CT-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.663AlaAla: 2.663 ± 0.14
0.404AlaCys: 0.404 ± 0.039
2.008AlaAsp: 2.008 ± 0.107
2.412AlaGlu: 2.412 ± 0.109
2.351AlaPhe: 2.351 ± 0.089
2.595AlaGly: 2.595 ± 0.109
0.577AlaHis: 0.577 ± 0.052
5.177AlaIle: 5.177 ± 0.144
4.546AlaLys: 4.546 ± 0.165
4.62AlaLeu: 4.62 ± 0.129
1.062AlaMet: 1.062 ± 0.072
3.06AlaAsn: 3.06 ± 0.095
1.103AlaPro: 1.103 ± 0.064
1.354AlaGln: 1.354 ± 0.068
1.455AlaArg: 1.455 ± 0.074
2.904AlaSer: 2.904 ± 0.106
2.456AlaThr: 2.456 ± 0.116
2.47AlaVal: 2.47 ± 0.102
0.421AlaTrp: 0.421 ± 0.042
1.567AlaTyr: 1.567 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.346CysAla: 0.346 ± 0.033
0.075CysCys: 0.075 ± 0.018
0.356CysAsp: 0.356 ± 0.034
0.458CysGlu: 0.458 ± 0.041
0.465CysPhe: 0.465 ± 0.039
0.509CysGly: 0.509 ± 0.04
0.115CysHis: 0.115 ± 0.02
0.617CysIle: 0.617 ± 0.052
0.617CysLys: 0.617 ± 0.053
0.617CysLeu: 0.617 ± 0.044
0.139CysMet: 0.139 ± 0.024
0.397CysAsn: 0.397 ± 0.042
0.176CysPro: 0.176 ± 0.025
0.193CysGln: 0.193 ± 0.026
0.129CysArg: 0.129 ± 0.018
0.533CysSer: 0.533 ± 0.036
0.288CysThr: 0.288 ± 0.033
0.332CysVal: 0.332 ± 0.033
0.075CysTrp: 0.075 ± 0.014
0.278CysTyr: 0.278 ± 0.031
0.0CysXaa: 0.0 ± 0.0
Asp
1.89AspAla: 1.89 ± 0.09
0.322AspCys: 0.322 ± 0.035
2.466AspAsp: 2.466 ± 0.105
3.993AspGlu: 3.993 ± 0.136
3.911AspPhe: 3.911 ± 0.129
2.436AspGly: 2.436 ± 0.099
0.529AspHis: 0.529 ± 0.038
5.092AspIle: 5.092 ± 0.145
4.542AspLys: 4.542 ± 0.136
5.879AspLeu: 5.879 ± 0.135
0.936AspMet: 0.936 ± 0.062
3.335AspAsn: 3.335 ± 0.105
1.279AspPro: 1.279 ± 0.064
1.669AspGln: 1.669 ± 0.082
1.164AspArg: 1.164 ± 0.063
3.474AspSer: 3.474 ± 0.108
1.842AspThr: 1.842 ± 0.086
2.578AspVal: 2.578 ± 0.098
0.638AspTrp: 0.638 ± 0.044
2.456AspTyr: 2.456 ± 0.109
0.0AspXaa: 0.0 ± 0.0
Glu
3.063GluAla: 3.063 ± 0.135
0.387GluCys: 0.387 ± 0.035
3.145GluAsp: 3.145 ± 0.119
5.859GluGlu: 5.859 ± 0.187
3.884GluPhe: 3.884 ± 0.134
2.629GluGly: 2.629 ± 0.112
0.773GluHis: 0.773 ± 0.058
9.271GluIle: 9.271 ± 0.201
8.779GluLys: 8.779 ± 0.195
7.29GluLeu: 7.29 ± 0.193
1.625GluMet: 1.625 ± 0.08
6.897GluAsn: 6.897 ± 0.172
1.387GluPro: 1.387 ± 0.074
2.551GluGln: 2.551 ± 0.095
1.795GluArg: 1.795 ± 0.081
3.311GluSer: 3.311 ± 0.102
3.26GluThr: 3.26 ± 0.097
3.922GluVal: 3.922 ± 0.127
0.662GluTrp: 0.662 ± 0.041
2.731GluTyr: 2.731 ± 0.101
0.0GluXaa: 0.0 ± 0.0
Phe
2.663PheAla: 2.663 ± 0.102
0.489PheCys: 0.489 ± 0.04
3.304PheAsp: 3.304 ± 0.109
4.349PheGlu: 4.349 ± 0.149
3.175PhePhe: 3.175 ± 0.118
2.989PheGly: 2.989 ± 0.123
0.495PheHis: 0.495 ± 0.046
6.028PheIle: 6.028 ± 0.197
5.692PheLys: 5.692 ± 0.162
6.079PheLeu: 6.079 ± 0.185
1.177PheMet: 1.177 ± 0.065
4.556PheAsn: 4.556 ± 0.127
1.086PhePro: 1.086 ± 0.056
1.547PheGln: 1.547 ± 0.077
1.35PheArg: 1.35 ± 0.071
4.749PheSer: 4.749 ± 0.127
2.802PheThr: 2.802 ± 0.106
2.826PheVal: 2.826 ± 0.098
0.672PheTrp: 0.672 ± 0.056
2.263PheTyr: 2.263 ± 0.102
0.0PheXaa: 0.0 ± 0.0
Gly
2.707GlyAla: 2.707 ± 0.111
0.434GlyCys: 0.434 ± 0.039
2.232GlyAsp: 2.232 ± 0.085
3.053GlyGlu: 3.053 ± 0.111
2.799GlyPhe: 2.799 ± 0.091
2.819GlyGly: 2.819 ± 0.129
0.699GlyHis: 0.699 ± 0.056
5.312GlyIle: 5.312 ± 0.136
4.437GlyLys: 4.437 ± 0.128
4.285GlyLeu: 4.285 ± 0.155
1.167GlyMet: 1.167 ± 0.062
2.67GlyAsn: 2.67 ± 0.105
1.069GlyPro: 1.069 ± 0.067
1.384GlyGln: 1.384 ± 0.066
1.442GlyArg: 1.442 ± 0.074
3.101GlySer: 3.101 ± 0.116
2.646GlyThr: 2.646 ± 0.101
3.158GlyVal: 3.158 ± 0.12
0.573GlyTrp: 0.573 ± 0.046
1.968GlyTyr: 1.968 ± 0.08
0.0GlyXaa: 0.0 ± 0.0
His
0.553HisAla: 0.553 ± 0.043
0.071HisCys: 0.071 ± 0.016
0.59HisAsp: 0.59 ± 0.049
0.651HisGlu: 0.651 ± 0.047
0.709HisPhe: 0.709 ± 0.052
0.723HisGly: 0.723 ± 0.059
0.193HisHis: 0.193 ± 0.024
1.031HisIle: 1.031 ± 0.06
0.991HisLys: 0.991 ± 0.061
1.086HisLeu: 1.086 ± 0.058
0.207HisMet: 0.207 ± 0.028
0.743HisAsn: 0.743 ± 0.051
0.461HisPro: 0.461 ± 0.044
0.319HisGln: 0.319 ± 0.032
0.377HisArg: 0.377 ± 0.042
0.723HisSer: 0.723 ± 0.056
0.465HisThr: 0.465 ± 0.041
0.604HisVal: 0.604 ± 0.047
0.119HisTrp: 0.119 ± 0.021
0.424HisTyr: 0.424 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
5.618IleAla: 5.618 ± 0.142
0.95IleCys: 0.95 ± 0.055
6.228IleAsp: 6.228 ± 0.155
8.389IleGlu: 8.389 ± 0.189
6.517IlePhe: 6.517 ± 0.206
5.075IleGly: 5.075 ± 0.134
1.136IleHis: 1.136 ± 0.055
11.442IleIle: 11.442 ± 0.243
10.598IleLys: 10.598 ± 0.223
10.153IleLeu: 10.153 ± 0.213
2.018IleMet: 2.018 ± 0.091
8.891IleAsn: 8.891 ± 0.189
2.554IlePro: 2.554 ± 0.099
2.792IleGln: 2.792 ± 0.101
2.697IleArg: 2.697 ± 0.093
8.545IleSer: 8.545 ± 0.209
5.041IleThr: 5.041 ± 0.135
5.638IleVal: 5.638 ± 0.138
1.004IleTrp: 1.004 ± 0.061
3.915IleTyr: 3.915 ± 0.134
0.0IleXaa: 0.0 ± 0.0
Lys
3.782LysAla: 3.782 ± 0.139
0.448LysCys: 0.448 ± 0.044
4.99LysAsp: 4.99 ± 0.121
8.993LysGlu: 8.993 ± 0.231
4.98LysPhe: 4.98 ± 0.139
3.542LysGly: 3.542 ± 0.126
1.038LysHis: 1.038 ± 0.055
12.246LysIle: 12.246 ± 0.253
11.483LysLys: 11.483 ± 0.3
8.868LysLeu: 8.868 ± 0.188
2.799LysMet: 2.799 ± 0.097
9.862LysAsn: 9.862 ± 0.188
2.205LysPro: 2.205 ± 0.089
3.291LysGln: 3.291 ± 0.124
2.968LysArg: 2.968 ± 0.124
5.621LysSer: 5.621 ± 0.134
5.34LysThr: 5.34 ± 0.147
5.139LysVal: 5.139 ± 0.152
0.97LysTrp: 0.97 ± 0.057
4.559LysTyr: 4.559 ± 0.131
0.0LysXaa: 0.0 ± 0.0
Leu
4.346LeuAla: 4.346 ± 0.143
0.539LeuCys: 0.539 ± 0.041
4.98LeuAsp: 4.98 ± 0.129
7.361LeuGlu: 7.361 ± 0.197
5.533LeuPhe: 5.533 ± 0.181
4.42LeuGly: 4.42 ± 0.14
0.93LeuHis: 0.93 ± 0.059
10.537LeuIle: 10.537 ± 0.218
10.649LeuLys: 10.649 ± 0.21
8.715LeuLeu: 8.715 ± 0.206
1.883LeuMet: 1.883 ± 0.079
8.576LeuAsn: 8.576 ± 0.188
2.259LeuPro: 2.259 ± 0.09
2.463LeuGln: 2.463 ± 0.082
2.517LeuArg: 2.517 ± 0.103
6.574LeuSer: 6.574 ± 0.16
5.122LeuThr: 5.122 ± 0.135
5.017LeuVal: 5.017 ± 0.123
0.807LeuTrp: 0.807 ± 0.058
2.999LeuTyr: 2.999 ± 0.1
0.0LeuXaa: 0.0 ± 0.0
Met
1.242MetAla: 1.242 ± 0.07
0.156MetCys: 0.156 ± 0.026
0.977MetAsp: 0.977 ± 0.055
1.299MetGlu: 1.299 ± 0.065
1.106MetPhe: 1.106 ± 0.051
1.221MetGly: 1.221 ± 0.076
0.312MetHis: 0.312 ± 0.034
1.981MetIle: 1.981 ± 0.087
2.317MetLys: 2.317 ± 0.086
1.713MetLeu: 1.713 ± 0.079
0.387MetMet: 0.387 ± 0.036
1.693MetAsn: 1.693 ± 0.084
0.628MetPro: 0.628 ± 0.044
0.818MetGln: 0.818 ± 0.062
0.485MetArg: 0.485 ± 0.04
1.35MetSer: 1.35 ± 0.067
1.014MetThr: 1.014 ± 0.071
1.113MetVal: 1.113 ± 0.062
0.183MetTrp: 0.183 ± 0.026
0.685MetTyr: 0.685 ± 0.051
0.0MetXaa: 0.0 ± 0.0
Asn
2.86AsnAla: 2.86 ± 0.112
0.431AsnCys: 0.431 ± 0.034
4.145AsnAsp: 4.145 ± 0.107
5.926AsnGlu: 5.926 ± 0.141
5.414AsnPhe: 5.414 ± 0.156
3.416AsnGly: 3.416 ± 0.125
0.794AsnHis: 0.794 ± 0.054
8.562AsnIle: 8.562 ± 0.177
8.298AsnLys: 8.298 ± 0.192
8.43AsnLeu: 8.43 ± 0.186
1.367AsnMet: 1.367 ± 0.068
6.741AsnAsn: 6.741 ± 0.178
1.995AsnPro: 1.995 ± 0.087
3.084AsnGln: 3.084 ± 0.133
1.9AsnArg: 1.9 ± 0.072
6.13AsnSer: 6.13 ± 0.174
2.897AsnThr: 2.897 ± 0.1
3.599AsnVal: 3.599 ± 0.122
0.946AsnTrp: 0.946 ± 0.069
3.83AsnTyr: 3.83 ± 0.119
0.0AsnXaa: 0.0 ± 0.0
Pro
0.919ProAla: 0.919 ± 0.058
0.132ProCys: 0.132 ± 0.023
1.092ProAsp: 1.092 ± 0.069
1.856ProGlu: 1.856 ± 0.07
1.309ProPhe: 1.309 ± 0.065
1.36ProGly: 1.36 ± 0.069
0.332ProHis: 0.332 ± 0.035
2.636ProIle: 2.636 ± 0.091
2.032ProLys: 2.032 ± 0.096
2.256ProLeu: 2.256 ± 0.085
0.451ProMet: 0.451 ± 0.042
1.598ProAsn: 1.598 ± 0.072
0.41ProPro: 0.41 ± 0.034
0.692ProGln: 0.692 ± 0.041
0.509ProArg: 0.509 ± 0.042
1.499ProSer: 1.499 ± 0.076
1.33ProThr: 1.33 ± 0.073
1.466ProVal: 1.466 ± 0.073
0.282ProTrp: 0.282 ± 0.034
0.913ProTyr: 0.913 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
1.272GlnAla: 1.272 ± 0.063
0.088GlnCys: 0.088 ± 0.02
1.299GlnAsp: 1.299 ± 0.071
2.412GlnGlu: 2.412 ± 0.092
1.493GlnPhe: 1.493 ± 0.076
1.123GlnGly: 1.123 ± 0.059
0.292GlnHis: 0.292 ± 0.028
3.599GlnIle: 3.599 ± 0.117
3.816GlnLys: 3.816 ± 0.116
2.911GlnLeu: 2.911 ± 0.106
0.607GlnMet: 0.607 ± 0.05
2.907GlnAsn: 2.907 ± 0.108
0.55GlnPro: 0.55 ± 0.047
0.974GlnGln: 0.974 ± 0.063
1.065GlnArg: 1.065 ± 0.063
1.581GlnSer: 1.581 ± 0.07
1.523GlnThr: 1.523 ± 0.069
1.411GlnVal: 1.411 ± 0.068
0.312GlnTrp: 0.312 ± 0.04
1.092GlnTyr: 1.092 ± 0.068
0.0GlnXaa: 0.0 ± 0.0
Arg
1.255ArgAla: 1.255 ± 0.068
0.156ArgCys: 0.156 ± 0.023
1.499ArgAsp: 1.499 ± 0.073
2.049ArgGlu: 2.049 ± 0.092
1.32ArgPhe: 1.32 ± 0.069
1.333ArgGly: 1.333 ± 0.07
0.349ArgHis: 0.349 ± 0.034
2.711ArgIle: 2.711 ± 0.097
2.972ArgLys: 2.972 ± 0.115
2.185ArgLeu: 2.185 ± 0.092
0.658ArgMet: 0.658 ± 0.047
2.164ArgAsn: 2.164 ± 0.093
0.699ArgPro: 0.699 ± 0.05
0.93ArgGln: 0.93 ± 0.065
0.977ArgArg: 0.977 ± 0.062
1.387ArgSer: 1.387 ± 0.069
1.323ArgThr: 1.323 ± 0.073
1.452ArgVal: 1.452 ± 0.073
0.237ArgTrp: 0.237 ± 0.033
1.055ArgTyr: 1.055 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
2.89SerAla: 2.89 ± 0.118
0.397SerCys: 0.397 ± 0.038
3.175SerAsp: 3.175 ± 0.126
4.176SerGlu: 4.176 ± 0.131
4.084SerPhe: 4.084 ± 0.132
3.569SerGly: 3.569 ± 0.104
0.719SerHis: 0.719 ± 0.046
7.45SerIle: 7.45 ± 0.188
7.09SerLys: 7.09 ± 0.163
6.608SerLeu: 6.608 ± 0.199
1.187SerMet: 1.187 ± 0.064
5.234SerAsn: 5.234 ± 0.18
1.371SerPro: 1.371 ± 0.076
2.208SerGln: 2.208 ± 0.097
1.798SerArg: 1.798 ± 0.082
4.977SerSer: 4.977 ± 0.183
3.474SerThr: 3.474 ± 0.116
3.331SerVal: 3.331 ± 0.109
0.736SerTrp: 0.736 ± 0.05
2.571SerTyr: 2.571 ± 0.104
0.0SerXaa: 0.0 ± 0.0
Thr
2.208ThrAla: 2.208 ± 0.09
0.275ThrCys: 0.275 ± 0.032
2.375ThrAsp: 2.375 ± 0.088
2.968ThrGlu: 2.968 ± 0.095
2.87ThrPhe: 2.87 ± 0.095
2.917ThrGly: 2.917 ± 0.108
0.583ThrHis: 0.583 ± 0.048
5.044ThrIle: 5.044 ± 0.124
4.41ThrLys: 4.41 ± 0.116
4.736ThrLeu: 4.736 ± 0.144
1.048ThrMet: 1.048 ± 0.063
3.606ThrAsn: 3.606 ± 0.118
1.51ThrPro: 1.51 ± 0.062
1.435ThrGln: 1.435 ± 0.071
1.343ThrArg: 1.343 ± 0.071
3.396ThrSer: 3.396 ± 0.118
2.975ThrThr: 2.975 ± 0.108
2.755ThrVal: 2.755 ± 0.118
0.492ThrTrp: 0.492 ± 0.046
1.805ThrTyr: 1.805 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
2.89ValAla: 2.89 ± 0.115
0.492ValCys: 0.492 ± 0.042
2.972ValAsp: 2.972 ± 0.104
3.738ValGlu: 3.738 ± 0.136
2.839ValPhe: 2.839 ± 0.096
2.938ValGly: 2.938 ± 0.108
0.611ValHis: 0.611 ± 0.044
5.526ValIle: 5.526 ± 0.14
4.844ValLys: 4.844 ± 0.139
4.759ValLeu: 4.759 ± 0.148
1.035ValMet: 1.035 ± 0.06
3.718ValAsn: 3.718 ± 0.124
1.364ValPro: 1.364 ± 0.078
1.269ValGln: 1.269 ± 0.063
1.459ValArg: 1.459 ± 0.065
3.62ValSer: 3.62 ± 0.1
2.714ValThr: 2.714 ± 0.122
2.951ValVal: 2.951 ± 0.121
0.427ValTrp: 0.427 ± 0.037
1.893ValTyr: 1.893 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.455TrpAla: 0.455 ± 0.039
0.058TrpCys: 0.058 ± 0.013
0.485TrpAsp: 0.485 ± 0.044
0.604TrpGlu: 0.604 ± 0.045
0.787TrpPhe: 0.787 ± 0.048
0.455TrpGly: 0.455 ± 0.037
0.092TrpHis: 0.092 ± 0.017
1.13TrpIle: 1.13 ± 0.059
1.058TrpLys: 1.058 ± 0.057
0.97TrpLeu: 0.97 ± 0.061
0.282TrpMet: 0.282 ± 0.03
0.885TrpAsn: 0.885 ± 0.06
0.187TrpPro: 0.187 ± 0.024
0.248TrpGln: 0.248 ± 0.029
0.217TrpArg: 0.217 ± 0.025
0.583TrpSer: 0.583 ± 0.047
0.55TrpThr: 0.55 ± 0.051
0.533TrpVal: 0.533 ± 0.042
0.187TrpTrp: 0.187 ± 0.026
0.424TrpTyr: 0.424 ± 0.04
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.581TyrAla: 1.581 ± 0.071
0.414TyrCys: 0.414 ± 0.038
2.11TyrAsp: 2.11 ± 0.086
2.677TyrGlu: 2.677 ± 0.094
2.744TyrPhe: 2.744 ± 0.094
1.886TyrGly: 1.886 ± 0.077
0.468TyrHis: 0.468 ± 0.036
3.81TyrIle: 3.81 ± 0.128
3.952TyrLys: 3.952 ± 0.122
4.067TyrLeu: 4.067 ± 0.138
0.641TyrMet: 0.641 ± 0.047
3.233TyrAsn: 3.233 ± 0.138
0.784TyrPro: 0.784 ± 0.051
1.109TyrGln: 1.109 ± 0.057
1.065TyrArg: 1.065 ± 0.066
2.989TyrSer: 2.989 ± 0.134
1.689TyrThr: 1.689 ± 0.076
1.764TyrVal: 1.764 ± 0.081
0.444TyrTrp: 0.444 ± 0.041
1.554TyrTyr: 1.554 ± 0.073
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 989 proteins (294780 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski