Amino acid dipepetide frequency for Bifidobacterium reuteri DSM 23975

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.173AlaAla: 14.173 ± 0.201
1.113AlaCys: 1.113 ± 0.04
7.852AlaAsp: 7.852 ± 0.12
6.275AlaGlu: 6.275 ± 0.116
3.454AlaPhe: 3.454 ± 0.072
9.36AlaGly: 9.36 ± 0.136
2.233AlaHis: 2.233 ± 0.061
5.742AlaIle: 5.742 ± 0.094
4.757AlaLys: 4.757 ± 0.11
10.311AlaLeu: 10.311 ± 0.135
3.103AlaMet: 3.103 ± 0.071
3.662AlaAsn: 3.662 ± 0.069
4.126AlaPro: 4.126 ± 0.094
4.396AlaGln: 4.396 ± 0.104
5.898AlaArg: 5.898 ± 0.103
6.876AlaSer: 6.876 ± 0.102
6.058AlaThr: 6.058 ± 0.112
8.799AlaVal: 8.799 ± 0.135
1.377AlaTrp: 1.377 ± 0.046
2.663AlaTyr: 2.663 ± 0.06
0.001AlaXaa: 0.001 ± 0.0
Cys
1.093CysAla: 1.093 ± 0.037
0.134CysCys: 0.134 ± 0.016
0.531CysAsp: 0.531 ± 0.026
0.474CysGlu: 0.474 ± 0.026
0.335CysPhe: 0.335 ± 0.022
0.992CysGly: 0.992 ± 0.037
0.232CysHis: 0.232 ± 0.016
0.451CysIle: 0.451 ± 0.027
0.225CysLys: 0.225 ± 0.017
0.774CysLeu: 0.774 ± 0.034
0.237CysMet: 0.237 ± 0.017
0.232CysAsn: 0.232 ± 0.018
0.477CysPro: 0.477 ± 0.028
0.231CysGln: 0.231 ± 0.018
0.598CysArg: 0.598 ± 0.032
0.556CysSer: 0.556 ± 0.03
0.505CysThr: 0.505 ± 0.023
0.693CysVal: 0.693 ± 0.034
0.133CysTrp: 0.133 ± 0.013
0.221CysTyr: 0.221 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
7.84AspAla: 7.84 ± 0.118
0.558AspCys: 0.558 ± 0.03
4.778AspAsp: 4.778 ± 0.102
4.687AspGlu: 4.687 ± 0.091
2.274AspPhe: 2.274 ± 0.058
6.151AspGly: 6.151 ± 0.113
1.3AspHis: 1.3 ± 0.045
3.32AspIle: 3.32 ± 0.07
2.416AspLys: 2.416 ± 0.068
5.195AspLeu: 5.195 ± 0.082
1.709AspMet: 1.709 ± 0.048
1.917AspAsn: 1.917 ± 0.06
3.508AspPro: 3.508 ± 0.064
2.005AspGln: 2.005 ± 0.059
3.454AspArg: 3.454 ± 0.081
3.73AspSer: 3.73 ± 0.066
3.648AspThr: 3.648 ± 0.072
4.994AspVal: 4.994 ± 0.079
1.067AspTrp: 1.067 ± 0.037
2.049AspTyr: 2.049 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
6.159GluAla: 6.159 ± 0.11
0.458GluCys: 0.458 ± 0.029
3.174GluAsp: 3.174 ± 0.072
3.102GluGlu: 3.102 ± 0.074
1.865GluPhe: 1.865 ± 0.055
3.788GluGly: 3.788 ± 0.083
1.737GluHis: 1.737 ± 0.051
2.647GluIle: 2.647 ± 0.063
1.976GluLys: 1.976 ± 0.062
5.252GluLeu: 5.252 ± 0.105
1.276GluMet: 1.276 ± 0.042
1.776GluAsn: 1.776 ± 0.052
2.703GluPro: 2.703 ± 0.073
2.637GluGln: 2.637 ± 0.054
4.182GluArg: 4.182 ± 0.088
3.439GluSer: 3.439 ± 0.063
3.387GluThr: 3.387 ± 0.078
3.443GluVal: 3.443 ± 0.071
0.69GluTrp: 0.69 ± 0.029
1.808GluTyr: 1.808 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.955PheAla: 3.955 ± 0.074
0.349PheCys: 0.349 ± 0.022
2.678PheAsp: 2.678 ± 0.074
1.743PheGlu: 1.743 ± 0.049
1.177PhePhe: 1.177 ± 0.048
3.277PheGly: 3.277 ± 0.074
0.784PheHis: 0.784 ± 0.033
1.714PheIle: 1.714 ± 0.055
1.137PheLys: 1.137 ± 0.038
2.774PheLeu: 2.774 ± 0.061
0.777PheMet: 0.777 ± 0.035
1.293PheAsn: 1.293 ± 0.041
1.367PhePro: 1.367 ± 0.044
0.954PheGln: 0.954 ± 0.034
1.71PheArg: 1.71 ± 0.05
2.113PheSer: 2.113 ± 0.054
2.365PheThr: 2.365 ± 0.056
2.614PheVal: 2.614 ± 0.069
0.466PheTrp: 0.466 ± 0.028
0.887PheTyr: 0.887 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
7.562GlyAla: 7.562 ± 0.116
0.782GlyCys: 0.782 ± 0.032
4.88GlyAsp: 4.88 ± 0.096
4.569GlyGlu: 4.569 ± 0.093
3.264GlyPhe: 3.264 ± 0.074
6.041GlyGly: 6.041 ± 0.127
1.763GlyHis: 1.763 ± 0.049
4.685GlyIle: 4.685 ± 0.08
3.807GlyLys: 3.807 ± 0.095
7.165GlyLeu: 7.165 ± 0.111
2.182GlyMet: 2.182 ± 0.063
2.76GlyAsn: 2.76 ± 0.068
2.539GlyPro: 2.539 ± 0.07
2.656GlyGln: 2.656 ± 0.07
4.733GlyArg: 4.733 ± 0.086
5.265GlySer: 5.265 ± 0.089
5.229GlyThr: 5.229 ± 0.094
6.385GlyVal: 6.385 ± 0.102
1.283GlyTrp: 1.283 ± 0.046
2.706GlyTyr: 2.706 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
2.331HisAla: 2.331 ± 0.058
0.226HisCys: 0.226 ± 0.018
1.675HisAsp: 1.675 ± 0.05
1.216HisGlu: 1.216 ± 0.042
0.633HisPhe: 0.633 ± 0.029
1.926HisGly: 1.926 ± 0.052
0.598HisHis: 0.598 ± 0.031
1.217HisIle: 1.217 ± 0.036
0.61HisLys: 0.61 ± 0.028
1.617HisLeu: 1.617 ± 0.052
0.595HisMet: 0.595 ± 0.03
0.671HisAsn: 0.671 ± 0.029
1.329HisPro: 1.329 ± 0.04
0.693HisGln: 0.693 ± 0.03
1.43HisArg: 1.43 ± 0.048
1.031HisSer: 1.031 ± 0.036
1.267HisThr: 1.267 ± 0.043
1.845HisVal: 1.845 ± 0.058
0.344HisTrp: 0.344 ± 0.021
0.618HisTyr: 0.618 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.821IleAla: 6.821 ± 0.105
0.584IleCys: 0.584 ± 0.028
4.173IleAsp: 4.173 ± 0.071
3.016IleGlu: 3.016 ± 0.069
1.382IlePhe: 1.382 ± 0.044
4.628IleGly: 4.628 ± 0.085
0.996IleHis: 0.996 ± 0.036
2.992IleIle: 2.992 ± 0.07
1.712IleLys: 1.712 ± 0.05
3.831IleLeu: 3.831 ± 0.086
1.222IleMet: 1.222 ± 0.047
1.841IleAsn: 1.841 ± 0.051
2.805IlePro: 2.805 ± 0.061
1.433IleGln: 1.433 ± 0.044
3.05IleArg: 3.05 ± 0.064
3.082IleSer: 3.082 ± 0.073
3.436IleThr: 3.436 ± 0.075
4.506IleVal: 4.506 ± 0.09
0.618IleTrp: 0.618 ± 0.03
1.213IleTyr: 1.213 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
4.789LysAla: 4.789 ± 0.109
0.195LysCys: 0.195 ± 0.015
2.557LysAsp: 2.557 ± 0.069
2.082LysGlu: 2.082 ± 0.068
0.918LysPhe: 0.918 ± 0.04
2.64LysGly: 2.64 ± 0.071
0.824LysHis: 0.824 ± 0.03
1.723LysIle: 1.723 ± 0.054
1.632LysLys: 1.632 ± 0.055
3.138LysLeu: 3.138 ± 0.072
0.818LysMet: 0.818 ± 0.034
1.382LysAsn: 1.382 ± 0.048
2.305LysPro: 2.305 ± 0.054
1.545LysGln: 1.545 ± 0.053
2.319LysArg: 2.319 ± 0.062
2.245LysSer: 2.245 ± 0.053
2.585LysThr: 2.585 ± 0.061
2.745LysVal: 2.745 ± 0.063
0.422LysTrp: 0.422 ± 0.026
1.113LysTyr: 1.113 ± 0.036
0.001LysXaa: 0.001 ± 0.001
Leu
10.335LeuAla: 10.335 ± 0.137
0.863LeuCys: 0.863 ± 0.037
6.139LeuAsp: 6.139 ± 0.091
4.497LeuGlu: 4.497 ± 0.081
3.155LeuPhe: 3.155 ± 0.075
6.946LeuGly: 6.946 ± 0.104
1.866LeuHis: 1.866 ± 0.053
4.875LeuIle: 4.875 ± 0.098
3.575LeuLys: 3.575 ± 0.062
7.813LeuLeu: 7.813 ± 0.138
2.204LeuMet: 2.204 ± 0.054
3.071LeuAsn: 3.071 ± 0.055
4.471LeuPro: 4.471 ± 0.078
2.408LeuGln: 2.408 ± 0.055
4.936LeuArg: 4.936 ± 0.095
5.498LeuSer: 5.498 ± 0.086
5.64LeuThr: 5.64 ± 0.084
6.38LeuVal: 6.38 ± 0.11
1.045LeuTrp: 1.045 ± 0.043
2.091LeuTyr: 2.091 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.817MetAla: 2.817 ± 0.057
0.226MetCys: 0.226 ± 0.015
1.376MetAsp: 1.376 ± 0.039
1.073MetGlu: 1.073 ± 0.037
0.852MetPhe: 0.852 ± 0.033
1.812MetGly: 1.812 ± 0.046
0.554MetHis: 0.554 ± 0.028
1.285MetIle: 1.285 ± 0.036
0.978MetLys: 0.978 ± 0.039
2.606MetLeu: 2.606 ± 0.06
0.725MetMet: 0.725 ± 0.033
0.952MetAsn: 0.952 ± 0.037
1.47MetPro: 1.47 ± 0.048
0.858MetGln: 0.858 ± 0.033
1.7MetArg: 1.7 ± 0.049
1.779MetSer: 1.779 ± 0.049
1.949MetThr: 1.949 ± 0.042
1.864MetVal: 1.864 ± 0.047
0.294MetTrp: 0.294 ± 0.019
0.502MetTyr: 0.502 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.978AsnAla: 3.978 ± 0.08
0.219AsnCys: 0.219 ± 0.015
2.21AsnAsp: 2.21 ± 0.059
1.843AsnGlu: 1.843 ± 0.052
0.863AsnPhe: 0.863 ± 0.036
3.31AsnGly: 3.31 ± 0.074
0.704AsnHis: 0.704 ± 0.031
1.63AsnIle: 1.63 ± 0.047
1.185AsnLys: 1.185 ± 0.041
2.727AsnLeu: 2.727 ± 0.067
0.798AsnMet: 0.798 ± 0.035
1.201AsnAsn: 1.201 ± 0.057
2.293AsnPro: 2.293 ± 0.06
1.155AsnGln: 1.155 ± 0.044
2.049AsnArg: 2.049 ± 0.05
1.709AsnSer: 1.709 ± 0.052
2.134AsnThr: 2.134 ± 0.058
2.393AsnVal: 2.393 ± 0.054
0.477AsnTrp: 0.477 ± 0.024
0.919AsnTyr: 0.919 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
5.164ProAla: 5.164 ± 0.092
0.312ProCys: 0.312 ± 0.021
3.529ProAsp: 3.529 ± 0.067
3.402ProGlu: 3.402 ± 0.07
1.74ProPhe: 1.74 ± 0.048
3.544ProGly: 3.544 ± 0.071
0.934ProHis: 0.934 ± 0.037
2.241ProIle: 2.241 ± 0.058
1.814ProLys: 1.814 ± 0.053
3.896ProLeu: 3.896 ± 0.076
1.106ProMet: 1.106 ± 0.042
1.619ProAsn: 1.619 ± 0.047
1.3ProPro: 1.3 ± 0.042
1.825ProGln: 1.825 ± 0.08
2.293ProArg: 2.293 ± 0.061
3.056ProSer: 3.056 ± 0.067
2.82ProThr: 2.82 ± 0.063
3.921ProVal: 3.921 ± 0.074
0.715ProTrp: 0.715 ± 0.032
1.454ProTyr: 1.454 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
3.832GlnAla: 3.832 ± 0.107
0.315GlnCys: 0.315 ± 0.019
1.64GlnAsp: 1.64 ± 0.044
1.509GlnGlu: 1.509 ± 0.047
1.137GlnPhe: 1.137 ± 0.041
2.399GlnGly: 2.399 ± 0.06
0.812GlnHis: 0.812 ± 0.03
1.891GlnIle: 1.891 ± 0.048
1.039GlnLys: 1.039 ± 0.036
3.402GlnLeu: 3.402 ± 0.067
0.992GlnMet: 0.992 ± 0.04
1.099GlnAsn: 1.099 ± 0.044
1.886GlnPro: 1.886 ± 0.088
1.71GlnGln: 1.71 ± 0.074
2.276GlnArg: 2.276 ± 0.054
2.347GlnSer: 2.347 ± 0.064
2.0GlnThr: 2.0 ± 0.054
2.456GlnVal: 2.456 ± 0.058
0.632GlnTrp: 0.632 ± 0.032
1.196GlnTyr: 1.196 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
5.211ArgAla: 5.211 ± 0.088
0.514ArgCys: 0.514 ± 0.028
3.516ArgAsp: 3.516 ± 0.086
3.665ArgGlu: 3.665 ± 0.08
2.326ArgPhe: 2.326 ± 0.061
3.696ArgGly: 3.696 ± 0.082
1.588ArgHis: 1.588 ± 0.05
3.689ArgIle: 3.689 ± 0.077
2.418ArgLys: 2.418 ± 0.062
5.559ArgLeu: 5.559 ± 0.104
1.752ArgMet: 1.752 ± 0.049
2.118ArgAsn: 2.118 ± 0.054
2.549ArgPro: 2.549 ± 0.059
2.205ArgGln: 2.205 ± 0.065
4.807ArgArg: 4.807 ± 0.116
3.271ArgSer: 3.271 ± 0.07
3.482ArgThr: 3.482 ± 0.062
4.055ArgVal: 4.055 ± 0.092
0.879ArgTrp: 0.879 ± 0.035
1.935ArgTyr: 1.935 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
6.685SerAla: 6.685 ± 0.107
0.502SerCys: 0.502 ± 0.029
4.069SerAsp: 4.069 ± 0.082
3.108SerGlu: 3.108 ± 0.065
2.164SerPhe: 2.164 ± 0.054
5.709SerGly: 5.709 ± 0.092
1.335SerHis: 1.335 ± 0.036
3.326SerIle: 3.326 ± 0.054
2.18SerLys: 2.18 ± 0.05
5.339SerLeu: 5.339 ± 0.08
1.653SerMet: 1.653 ± 0.042
2.087SerAsn: 2.087 ± 0.052
2.468SerPro: 2.468 ± 0.064
2.113SerGln: 2.113 ± 0.06
3.547SerArg: 3.547 ± 0.07
4.28SerSer: 4.28 ± 0.1
3.739SerThr: 3.739 ± 0.076
4.484SerVal: 4.484 ± 0.077
0.914SerTrp: 0.914 ± 0.038
1.622SerTyr: 1.622 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
6.778ThrAla: 6.778 ± 0.095
0.497ThrCys: 0.497 ± 0.027
4.008ThrAsp: 4.008 ± 0.074
2.904ThrGlu: 2.904 ± 0.058
2.115ThrPhe: 2.115 ± 0.06
5.357ThrGly: 5.357 ± 0.092
1.207ThrHis: 1.207 ± 0.041
3.546ThrIle: 3.546 ± 0.078
2.149ThrLys: 2.149 ± 0.068
5.625ThrLeu: 5.625 ± 0.083
1.535ThrMet: 1.535 ± 0.042
1.938ThrAsn: 1.938 ± 0.054
3.448ThrPro: 3.448 ± 0.079
1.98ThrGln: 1.98 ± 0.047
3.103ThrArg: 3.103 ± 0.073
3.518ThrSer: 3.518 ± 0.076
3.728ThrThr: 3.728 ± 0.085
5.696ThrVal: 5.696 ± 0.091
0.828ThrTrp: 0.828 ± 0.034
1.642ThrTyr: 1.642 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
8.301ValAla: 8.301 ± 0.12
0.79ValCys: 0.79 ± 0.037
5.056ValAsp: 5.056 ± 0.091
4.109ValGlu: 4.109 ± 0.086
2.911ValPhe: 2.911 ± 0.063
5.112ValGly: 5.112 ± 0.087
1.514ValHis: 1.514 ± 0.045
4.451ValIle: 4.451 ± 0.083
2.963ValLys: 2.963 ± 0.072
6.869ValLeu: 6.869 ± 0.128
1.963ValMet: 1.963 ± 0.053
2.692ValAsn: 2.692 ± 0.057
3.807ValPro: 3.807 ± 0.072
2.164ValGln: 2.164 ± 0.058
4.353ValArg: 4.353 ± 0.084
5.065ValSer: 5.065 ± 0.102
5.221ValThr: 5.221 ± 0.093
6.303ValVal: 6.303 ± 0.112
1.024ValTrp: 1.024 ± 0.038
1.88ValTyr: 1.88 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
1.219TrpAla: 1.219 ± 0.043
0.169TrpCys: 0.169 ± 0.015
0.793TrpAsp: 0.793 ± 0.031
0.605TrpGlu: 0.605 ± 0.03
0.589TrpPhe: 0.589 ± 0.03
0.947TrpGly: 0.947 ± 0.04
0.369TrpHis: 0.369 ± 0.023
0.692TrpIle: 0.692 ± 0.031
0.555TrpLys: 0.555 ± 0.025
1.505TrpLeu: 1.505 ± 0.049
0.431TrpMet: 0.431 ± 0.022
0.616TrpAsn: 0.616 ± 0.029
0.538TrpPro: 0.538 ± 0.029
0.608TrpGln: 0.608 ± 0.029
1.0TrpArg: 1.0 ± 0.041
0.877TrpSer: 0.877 ± 0.035
0.781TrpThr: 0.781 ± 0.032
0.928TrpVal: 0.928 ± 0.033
0.242TrpTrp: 0.242 ± 0.02
0.445TrpTyr: 0.445 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.186TyrAla: 3.186 ± 0.076
0.322TyrCys: 0.322 ± 0.025
1.963TyrAsp: 1.963 ± 0.051
1.62TyrGlu: 1.62 ± 0.048
0.98TyrPhe: 0.98 ± 0.042
2.62TyrGly: 2.62 ± 0.072
0.52TyrHis: 0.52 ± 0.024
1.222TyrIle: 1.222 ± 0.04
0.851TyrLys: 0.851 ± 0.034
2.41TyrLeu: 2.41 ± 0.058
0.594TyrMet: 0.594 ± 0.029
0.869TyrAsn: 0.869 ± 0.034
1.296TyrPro: 1.296 ± 0.038
0.996TyrGln: 0.996 ± 0.037
1.797TyrArg: 1.797 ± 0.051
1.589TyrSer: 1.589 ± 0.042
1.625TyrThr: 1.625 ± 0.051
2.045TyrVal: 2.045 ± 0.056
0.456TyrTrp: 0.456 ± 0.027
0.856TyrTyr: 0.856 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2149 proteins (805358 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski