Gordonia phage Petra
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8UK99|A0A2U8UK99_9CAUD Major capsid protein OS=Gordonia phage Petra OX=2182347 GN=6 PE=4 SV=1
MM1 pKa = 8.82 IMINPSNVADD11 pKa = 4.41 FHH13 pKa = 6.56 AAGEE17 pKa = 4.45 GTYY20 pKa = 8.34 WWHH23 pKa = 7.95 DD24 pKa = 3.75 SATDD28 pKa = 3.6 AYY30 pKa = 11.05 LVTDD34 pKa = 4.16 TFDD37 pKa = 3.95 PPTTDD42 pKa = 3.64 SVYY45 pKa = 11.0 LMPWDD50 pKa = 4.86 GPWFAQWGEE59 pKa = 3.78 NWAAAADD66 pKa = 3.82 QLNSVIARR74 pKa = 11.84 QEE76 pKa = 3.94 EE77 pKa = 4.71 EE78 pKa = 4.19 PTDD81 pKa = 3.68 GG82 pKa = 4.78
Molecular weight: 9.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 3.554
IPC_protein 3.49
Toseland 3.3
ProMoST 3.706
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.49
Rodwell 3.338
Grimsley 3.21
Solomon 3.465
Lehninger 3.427
Nozaki 3.656
DTASelect 3.859
Thurlkill 3.389
EMBOSS 3.503
Sillero 3.63
Patrickios 0.604
IPC_peptide 3.465
IPC2_peptide 3.592
IPC2.peptide.svr19 3.638
Protein with the highest isoelectric point:
>tr|A0A2U8UL46|A0A2U8UL46_9CAUD Uncharacterized protein OS=Gordonia phage Petra OX=2182347 GN=43 PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 APSTPHH8 pKa = 5.57 TPMLGWHH15 pKa = 5.67 QPCGDD20 pKa = 3.54 RR21 pKa = 11.84 ATPHH25 pKa = 6.05 RR26 pKa = 11.84 TDD28 pKa = 2.92 THH30 pKa = 6.36 AAPAPRR36 pKa = 11.84 RR37 pKa = 11.84 APTPHH42 pKa = 5.98 TRR44 pKa = 11.84 RR45 pKa = 11.84 PRR47 pKa = 11.84 MAPRR51 pKa = 11.84 KK52 pKa = 6.2 TTTQKK57 pKa = 10.8 HH58 pKa = 6.09 LGWSHH63 pKa = 4.95 QQNARR68 pKa = 11.84 HH69 pKa = 6.13 LKK71 pKa = 8.37 AQHH74 pKa = 6.47 VDD76 pKa = 3.7 GTPCWWCGRR85 pKa = 11.84 PMWRR89 pKa = 11.84 DD90 pKa = 3.09 RR91 pKa = 11.84 TRR93 pKa = 11.84 NYY95 pKa = 10.76 DD96 pKa = 3.91 YY97 pKa = 10.93 NPHH100 pKa = 5.65 STDD103 pKa = 3.42 PASGSLAADD112 pKa = 3.47 HH113 pKa = 5.91 SHH115 pKa = 6.52 PRR117 pKa = 11.84 AHH119 pKa = 7.46 GGRR122 pKa = 11.84 KK123 pKa = 9.13 ADD125 pKa = 4.06 RR126 pKa = 11.84 LLHH129 pKa = 5.08 GQCNKK134 pKa = 9.76 EE135 pKa = 3.85 RR136 pKa = 11.84 GAGNHH141 pKa = 5.29 DD142 pKa = 3.71 HH143 pKa = 6.42 QRR145 pKa = 11.84 PALTGTQPDD154 pKa = 3.92 TAPNNLGPLAITCWPP169 pKa = 3.6
Molecular weight: 18.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.589
IPC_protein 10.701
Toseland 10.906
ProMoST 10.935
Dawson 10.95
Bjellqvist 10.774
Wikipedia 11.257
Rodwell 10.95
Grimsley 10.994
Solomon 11.228
Lehninger 11.169
Nozaki 10.921
DTASelect 10.774
Thurlkill 10.891
EMBOSS 11.345
Sillero 10.921
Patrickios 10.701
IPC_peptide 11.228
IPC2_peptide 10.218
IPC2.peptide.svr19 8.886
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
89
0
89
17012
53
1830
191.1
20.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.527 ± 0.429
0.958 ± 0.148
6.883 ± 0.326
5.602 ± 0.311
2.822 ± 0.149
8.47 ± 0.331
2.375 ± 0.195
4.644 ± 0.161
3.321 ± 0.242
7.601 ± 0.27
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.216 ± 0.185
2.933 ± 0.149
5.937 ± 0.252
3.445 ± 0.148
7.689 ± 0.373
5.073 ± 0.177
6.713 ± 0.241
7.371 ± 0.258
2.063 ± 0.119
2.357 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here