Proteome-pI 2.0 - Proteome Isoelectric Point Database

Methanosphaera sp. rholeuAM6

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; unclassified Methanosphaera

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A328RXT7|A0A328RXT7_9EURY Uncharacterized protein (Fragment) OS=Methanosphaera sp. rholeuAM6 OX=1945580 GN=BZ135_08995 PE=4 SV=1

MM1 pKa = 7.63LLLIVFTITITLSSVNAGLFDD22 pKa = 5.68LDD24 pKa = 4.42STNDD28 pKa = 4.2DD29 pKa = 3.57INVTDD34 pKa = 5.19LEE36 pKa = 4.46INYY39 pKa = 9.27EE40 pKa = 4.25GYY42 pKa = 8.12STYY45 pKa = 10.81EE46 pKa = 4.25VNCKK50 pKa = 8.27LTPKK54 pKa = 10.41KK55 pKa = 10.81DD56 pKa = 3.44FDD58 pKa = 4.04YY59 pKa = 11.82LEE61 pKa = 4.36MFVIFYY67 pKa = 10.77DD68 pKa = 3.74SDD70 pKa = 3.96DD71 pKa = 3.83AVLDD75 pKa = 3.63KK76 pKa = 10.95STLVWNTNQPTKK88 pKa = 10.85DD89 pKa = 3.32QVIKK93 pKa = 11.07VSGTAYY99 pKa = 9.78VQNDD103 pKa = 3.11NEE105 pKa = 4.34KK106 pKa = 9.12PVRR109 pKa = 11.84AEE111 pKa = 3.96VYY113 pKa = 8.82FTDD116 pKa = 4.62GLDD119 pKa = 3.97AEE121 pKa = 4.85PEE123 pKa = 4.08SAIYY127 pKa = 10.15SEE129 pKa = 4.28NVTMGG134 pKa = 3.03

Molecular weight: 15.11 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A328RY80|A0A328RY80_9EURY Uncharacterized protein (Fragment) OS=Methanosphaera sp. rholeuAM6 OX=1945580 GN=BZ135_03755 PE=4 SV=1

MM1 pKa = 7.43SRR3 pKa = 11.84NKK5 pKa = 10.09YY6 pKa = 9.7LPKK9 pKa = 10.37KK10 pKa = 10.31LRR12 pKa = 11.84LAKK15 pKa = 10.19KK16 pKa = 8.58NKK18 pKa = 7.27QNRR21 pKa = 11.84RR22 pKa = 11.84VPIFAMMKK30 pKa = 8.74TSRR33 pKa = 11.84KK34 pKa = 9.59LRR36 pKa = 11.84THH38 pKa = 6.39PKK40 pKa = 7.85ARR42 pKa = 11.84QWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 10.85IKK50 pKa = 10.31VV51 pKa = 3.11

Molecular weight: 6.3 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1750	0	1750	513664	25	3340	293.5	33.05

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.383 ± 0.072	1.156 ± 0.026	6.311 ± 0.051	7.302 ± 0.092	3.797 ± 0.045	5.96 ± 0.072	1.653 ± 0.028	9.491 ± 0.067	7.65 ± 0.085	8.484 ± 0.078

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.325 ± 0.033	6.989 ± 0.115	3.033 ± 0.036	2.698 ± 0.041	3.199 ± 0.046	6.293 ± 0.058	6.6 ± 0.125	6.704 ± 0.067	0.583 ± 0.018	4.371 ± 0.059

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski